Maintenance Training

Education

● MS in Biochemistry and Molecular Biology (2010-2011)

Georgetown University

Washington DC

● Bachelor of Technology in Biotechnology (2006-2010) (First class with distinction)

Dr. D. Y. Patil University

Mumbai, India

Professional Experience

1.Georgetown University, School of Medicine (June 2011- present)

Department of Biochemistry and Molecular Biology,

Washington DC

Research Intern

Evaluation of CD133 as a putative melanoma stem cells marker using patient-derived tissue by obtaining positive and negative stem cells population of melanoma stem cells and sorting the cells by using Fluorescence-activated cell sorting (FACS) with CD133 as Stem Cell Specific Marker.

Studied in-vivo effect of sorted population in Nude Immunosuppressed Mice.

Immunohistochemistry of Paraffin fixed tumor tissue using CD133 marker.

2. Disha Life Sciences / Gujarat Cancer Research Institute (January 2010-July 2010)

Ahemdabad, India

Research Intern

Isolated Stem Cells from of Umbilical cord Blood and Wharton’s Jelly using Immunomagnetic sorting.

Extracted Phytochemical from Sygyzium Cumini and studied its effect on stem cells using Reverse Transcriptase PCR

3.SamourPharma (December 2007-January 2008)

Mumbai, India

Research Trainee

Performed Taste Masking of Azithromycin and Erthromycin by using Fluid Bed Processor

Formulated and prepared Placebo Tablets.

Formulated Phosphodiesterase type IV inhibitor(Mouth Spray).

4.Haffkine Institute of Technology (July 2008)

Department of Virology

Mumbai, India

Performed Flow Cytometry for HIV Antibody

Training taken in RT-PCR

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Technical Skills

1. Cytogenetic and Cancer genetics skills:

Immunohistochemistry

Immunocytochemistry

Fluorescence Microscopy,Phase Contrast Microscopy

Immunomagnetic Sorting

Fluorescence activated cell sorting

2. Biochemical and Molecular Biological:

Expression cloning

Primer designing, PCR and Reverse Transcriptase PCR

Isolation of RNA

SDS PAGE and Agarose Gel Electrophoresis

Western blotting and Southern blotting analysis

Transformation

Transduction

3. Cell culture:

Culturing and Maintenance of Human Hematopoietic stem cells.

Culturing and Maintenance of Human Melanoma Stem cells as a suspension cell Culture

Culturing and maintenance of Fibroblast cell line.

Coating of tissue culture plates using Polyhema

Basic Culturing: Feeding, Passaging, freezing,thawing.

4. Animal handling skills:

Injecting Human melanoma cells in Nude immunosuppressed Mice.

Feeding and maintaining the Mice.

Dissecting Tumors from Mice.

5. Computer skills:

Microsoft Word, Excel and PowerPoint, Mac OSX

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