Mechanical Medical

Xu, Edward

H: 908-***-**** M: 860-***-****

**** ******* **. ***** ****, NJ 08502 ********@*****.***

Professional Summary

Experienced research scientist in both industry and academy institute. Certified histotechnologist (ASCP). Extensive background in in-vivo experiment, bone biology, tissue regeneration and histopathology. Expertise includes histological techniques, Immunohistochemistry, animal handling, histopathological evaluation and some basic molecular biology techniques.

Licenses

Histotechnologist (HTL) certified by ASCP (American Society for Clinical Pathology)

Skill Highlights

• Experience with histological procedures: trims wet tissues, decalcification, fixation, embeds tissues, sections/microtome and routine staining.

• Hard tissue (bone) processing, MMA (plastics) embedding, sectioning and special staining.

• Immunohistochemistry.

• In-situ hybridization, In-situ RT-PCR, Isolation of RNA, western blot, cell culture and ELISA.

• Strong background to evaluate tissue grossly and microscopically from in-vivo animal models to human clinical samples.

• In vivo animal study design, animal handling and surgery.

• Bone Histomorphormetry evaluation by OsteoMetrics image analysis system.

• Image quantification via Nikon NIS-Element Basic Research System.

Professional Experience

LifeCell Corporation (2008-2012) Branchburg, NJ

Staff Scientist

● Worked in a multidisciplinary environment exploring mechanisms related to regenerative medicine, primarily through in vivo animal studies (rat, monkey and porcine models).

● Evaluated explant tissue grossly and sampled the tissues from in-vivo animal models to human clinical samples.

● Read histology slides to determine tissue (acellular collagen matrix) regeneration success or failure via observing fibroblast repopulation, revascularization and inflammatory response.

● Organized and summarized data in a logical format, which represented the overall conclusion regarding testing implants and biologic significance.

● Developed special staining or identify biomarker as required.

● Participated quality control procedure by histological analysis (scoring product sample) for product release.

● Compared different products for marketing on evidence based pathological analysis companied by representative images.

● Supported histology lab by providing feedback to correct artifacts and prevent reoccurrence of technical problems in slide preparation and to ensure accuracy and compliance with protocols or GLP regulation or SOPs.

● Explored correlation between image quantification and histopathological evaluation via Nikon NIS-Element Basic Research image analysis system.

Winthrop University Hospital (2006-2008) Mineola, NY

Research Associate

● Designed and carried out in vivo animal (rat) experiment for bone biology study.

● Responsible for animal dosing, necropsy, tissue processing, regular paraffin sectioning, no-decalcified bone sectioning, HE staining and special staining.

● Evaluated bone slides via histomorphormetry measurement system (OsteoMetrics image analysis system).

● Quantified bone biomarkers via radio immunoassay (RIA) and ELISA

● Summarized and interpreted data and communicated data with clinical departments.

● The data generated were successfully used in application for research funding.

● Maintained and operated laboratory equipments such as microtome and microscopy.

Pfizer Inc. (2001-2005) Groton, CT

Scientist

● Conducted in vivo studies to identify anti-osteoporosis agents for drug discovery on ovariectomy (OVX) and orchiectomy (ORX) rat models.

● Created animal model by surgery and dosed animal (oral, s.c. and IP) to test or screen the compounds.

● Responsible for regular paraffin sectioning, non-decalcified bone (large tissue) sectioning, HE staining, special staining and immunostain for bone biomarkers.

● Evaluated regular HE slides and special stain slides such as Trichrome, VonKossa, and Toludine Blue.

● Performed bone histomorphormetry measurement (OsteoMetrics image analysis system).

● Bone scanning by equipments: DEXA, pQCT and micro-CT.

● Conducted data analysis, made presentation and interacted with scientists and managers from other functional lines such as animal care, PK study, regulation group and project management.

● Maintained and operated laboratory equipments such as tissue processor, grinder/polisher, pQCT and micro-CT.

SUNY at Stony Brook (1996-2001) Stony Brook, NY

Research Associate

● Conducted in vivo studies to investigate cellular mechanism of bone response to mechanical stimuli.

● Performed mechanical loading on rat, mouse and turkey model (suspension model) with vibration plate (whole body vibration).

● Responsible for regular paraffin sectioning, non-decalcified bone (large tissue) sectioning and special staining such as TRAP and Trichrome.

● Evaluated bone slides via histomorphormetry measurement system (OsteoMetrics image analysis system) to see how the bone cell response to the certain mechanical loading environment.

● Identified up and down regulated genes involved in mechanical stimuli with Differential Display technology.

● Performed immunostain and in-situ RT-PCR on the paraffin sections for biomarkers.

● Maintained and operated histological laboratory equipments such as sharpener, grinder/polisher, embedding units and microtome.

● The data generated were successfully used in application for research funding.

Beijing Friendship Hospital (1986-1994) Beijing, China

Pathologist

● Engage in diagnosis of general surgical pathology. Check more than 20,000 cases of biopsy and perform 200 cases of autopsy.

● Perform immunohistochemistry and electromicroscopy for diagnosis and studies of cancers.

● Conduct in-vivo studies to identify agents to prevent precancerous lesion in esophagus.

● Evaluate esophagus tissue from rat model microscopically.

● Conduct clinical trial in the area with high incidence of esophagus cancer.

Education

City University of New York New York, US

Master degree in molecular biology

Beijing Medical University Beijing, China

Medical degree in Basic Medical Science

Additional Information

Thesis for M.S. degree: Construction of GFP vectors to study the localization of hnRNP A1 and A2 during cellular senescence.

1. Successfully accomplished the construction of expression vector pGFP that produces the fluorescent-tagged fusion protein.

2. Successfully made the transfection with pGFP vectors.

3. Performed subcloning, transfection, cell culture and westernblot.

Accomplishments

1. Jizu Zhi, G.Xu and C.Rubin. (2008) The lipogenic gene Spot 14 is activated in bone by disuse yet remains unaffected by a mechanical signal anabolic to the skeleton. Calcif. Tissue Int. 82:148-154

2. DG.Zhu, G.Xu, S.Ghandhi and K.Hubbard. (2002) Modulation of the Expression of p16INK4a and p14ARF by hnRNP A1 and A2 RNA Binding Proteins: Implications for Cellular Senescence. JOURNAL of CELLULAR PHYSIOLOGY 193:19-25

3. C.Rubin, G.Xu, and S.Judex. (2001) The anabolic activity of bone tissue, suppressed by disuse, is normalized by brief exposure to extremely low magnitude mechanical stimuli The FASEB JOURNAL 15:2225-2229.

4. C.Rubin, J.Zhi, G.Xu, K.Mcleod and M.Hadjiargyrou. (1998) Expression of novel gene products up regulated by disuse is normalized by an osteogenic mechanical stimulus: evidence for the molecular basis of a low level biomechanical countermeasure for osteoporosis? FIRST BIENNIAL SPACE BIOMEDICAL INVESTIGATORS’ WORKSHOP: 80-83

5. G.Xu and S.F.Huang (1993) Pathological observation of eight cases of carotid body tumor. CHINESE JOURNAL of PATHOLOGY. Vol.22 (1): p62.

6. G.Xu and S.Chen (1990) AIDS-the story of a disease and questions & answers on AIDS. (Translation from English to Chinese, over 180,000 words. Published by Knowledge Pressing House in China).

7. G.Xu (1983) Pathologcal analysis of 106 cases of large intestine cancer among youth. Collection of Beijing Medical University Graduation Thesis: 12-18.

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