Associate Scientist, Medical and Scientific expertise

TECHNICAL SKILLS:

Lab Techniques:

|RT-qPCR |Mammalian Cell Culture |

|DNA Extraction/Isolation (Wako kit,|Cell based Bioassays |

|Magmax Express-96) |Receptor binding assays, FRETs |

|Enzyme based assays |Western and Southern blots |

|Gel electrophoresis (SDS-PAGE) |Protein Purification Chromatography |

Lab Equipment and Software:

|MagMax Express-96 |Victor Wallac |

|ABI RT-PCR Systems (7300, 7900HT) |Meso Scale Discovery Reader |

|Nanodrop |Envision Multilabel Reader |

|Vi-cell Analyzer |Labware LIMS |

|SoftMax Pro |Nugenesis 7.0 |

Computer Software: Microsoft Excel (statistical analysis),

Word, PowerPoint, Outlook and PLA.

EXPERIENCE:

Associate Scientist I - Biogen Idec, USA- September 2010 -

Present:

. Member of the Bioassay Testing Group in Analytical

Development.

. Provided key support and fast turnaround for sample testing

according to SOPs and internal method protocols.

. Efficiently executed FcRn binding assays (Alphascreen

proprietary technology), FRET, ProteinA binding ELISAs, DNA

Isolation and Quantitative RT-PCR.

. Led the inter-departmental Assay Transfer activities for the

FcRn binding and the DNA Isolation and qPCR assays.

. Produced method qualification reports after qualifying the

qPCR assay for incoming projects.

. Provided training and guidance to colleagues on qPCR assays,

Ligand binding assays.

. Co-developed MS-Excel based paperless e-forms for various

assays in the group.

. Collaborated on an Antibody Dependent Cell Cytotoxicity

(ADCC) assay development.

. Maintained multiple human cell cultures simultaneously during

ADCC development work.

Summer Intern - Biogen Idec, USA- June 2010 - September 2010:

. Supported activities in the Bioassay Development Group in

Analytical Development.

. Optimized the automated DNA extraction protocol and software.

. Performed qualification work for a project on the DNA

extraction and quantitation method.

. Suggested modifications of existing protocols accordingly

throughout the assignment.

Development Specialist I (Co-op) - Shire Pharmaceuticals, USA-

January 2009 - August 2009:

. Worked for a Senior Scientist for the Immunoassay Development

Group in Pharmaceutical Analytical Development.

. Designed and performed experiments to troubleshoot enzyme

assays and transferred the optimized assay to the Testing

group.

. Worked with immunoblots (SDS-PAGE, Western Blots) and

prepared buffers and reagents in the process.

. Evaluated the AlphaLISA technology to measure protein

analytes.

Tutor - Worcester Polytechnic Institute, USA- August 2007 - May

2010:

. Advised students at the university with their course papers,

application essays, lab-reports, power-point presentations.

. Designed and held workshops for students on different writing

concepts.

. Created advertisements for the writing center and spread

awareness on campus of the services offered.

Activities: Writing, Soccer, Cricket, Basketball and Volunteering in

the local community.

EDUCATION:

Worcester Polytechnic Institute, Worcester, MA - USA

Bachelor of Science in Biology and Biotechnology (May,

2010)

-Awarded the International Student scholarship (USD

14,000/year) renewable for 4 years.

Major Qualifying Project:

Tested the effects of phytoestrogen components (Genistein) on

cellular proliferation using a well characterized estrogen

responsive breast epithelial cell line (T47D). Found conflicting

results with different concentrations of Genistein showing

distinct effects.

A young and dynamic biotechnology professional with a keen analytical mind.

Highly skilled at several molecular biology techniques and a technology

enthusiast with an eye for assay efficiencies and optimization. Deeply

rooted work ethics in a multidisciplinary team oriented environment, with

experience to contribute under tight deadlines as a team member and as an

individual.

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