Engineering Project

YAOYAO FU

*** ****** ****, *** ******* Drive, Durham, NC, 27708 y4yp8m@r.postjobfree.com 919-***-****

EDUCATION

DUKE UNIVERSITY, Pratt School of Engineering, Durham, NC. Aug 2009-Dec 2010

Master of Science Degree in Biomedical Engineering

FUDAN UNIVERSITY, Shanghai, China Sep 2004-June 2008

Bachelor of Science Degree in Biology

SKILLS

• Techniques: gene cloning, genotyping, gene sequencing, microarray, mammalian cell culture, flow cytometry, RNAi, real-time qPCR, confocal fluorescence imaging, western blot, nuclear magnetic resonance, IHC,ELISAs

• Computer programs: Vector NTI, Genomatix, MatLab, Mathematic, MS word, Powerpoint and Excel.

• Language: mandarin.

COURSE PROJECT EXPERIENCE

Biomedical Optical Diagnostics:

Created a tissue model for the skin and used Monte Carlo theory as an analytical model to investigate the instrument sensitivity required to measure blood glucose levels. The approach is aimed to develop non-invasive diagnostics of diabetes.

Biology by Design:

Co-designed a drug delivery vehicle consisting of a conjugated “lock and key” domain on a DNA box to selectively open the structure only when it contacts its target receptor tissue. This approach can be applied for localized drug delivery and thus limits side effects.

BIOTECH EXPERIENCE

Medical Center, DUKE UNIVERSITY, NC June 2010-Present

Independent research project: Immunomodulatory and antimicrobial drug screen and target identification.

• Screened immune activators and inhibitors using pathogen treated C. elegans and the neurotransmitter libraries.

• Performed RNAi to investigate the molecular mechanisms by which the activators and inhibitors work.

• Conducted pathogen-mediated killing assays to examine whether the drugs enhance the resistance of worms.

• Quantified GFP signals through flow cytometry.

• Quantified gene F35E12.5 expression through real time qPCR.

• Identified drug targets by using mutant animals deficient in dopamine or serotonin receptors in specific types of neurons.

Dept. of Biomedical Engineering, DUKE UNIVERSITY, NC Aug 2009-May 2010

Independent study project: Engineering an Efficient Biopathway for the Bioplastic polymer 3-hydroxybutyrate-co-4-hydroxybutyrate (3HB-co-4HB) (metabolic engineering).

• Sequenced genes responsible for co-polymer synthesis after circular polymerase extension cloning.

• Performed western blot to examine the expression of the genes encoding the enzymes responsible for the co-polymer synthesis

• Optimized the codons to enhance the production of one of the enzymes in order to get higher ratio of 4HB/3HB because 4HB is responsible for the physical property of the bioplastic.

• Gained competence in using NMR Spectroscopy to characterize the synthesized co-polymer.

Dept. of Neuroscience, STONY BROOK UNIVERSITY, NY Aug 2008-May 2009

• Cultured presynaptic ventral hippocampal (vHipp) neurons to investigate whether the expression of α7 nicotinic acetylcholine receptors(nAChR) can be influenced by Neuregulin1 using real-time quantitative PCR.

• Stained α7 nAChRs along axonal surface to see whether its distribution can be influenced by Nrg1 using immunostaining.

Institute of Brain Science, FUDAN UNIVERSITY, Shanghai, China Nov 2006-June 2008 Chun-Tsung Fellowship Award: Mitogen-Activated Protein Kinase (MAPK)/extracellular signal-regulated kinase (ERK) signal pathway in the development of cancer-induced pain.

• Promoted the rat model of cancer pain

• Performed ELISAs to examine the specific marker for activated microglia.

• Investigated the morphological change of microglia during the development of cancer pain by immunohistochemistry and confocal imaging.

• Utilized real-time qPCR to examine p38 MAP kinase gene expression at different time points during cancer pain development.

TEACHING EXPERIENCE

Duke University Dept. of Biomedical Engineering Teaching Assistant Feb 2010-May 2010

• Provided tutoring and grading to students in Genome Science and Technology

Duke University Dept. of Mathematics Teaching Assistant Aug 2009-Dec 2009

• Provided tutoring and grading to students in Introduction to Calculus II and Lab Calculus.

Fudan University, Shanghai, China Volunteer Tutor Sep 2004-July 2007

• Devised alternative strategies for teaching reading and math skills to disadvantaged students

COMMUNITY ACTIVITIES

Planning Committee, Women in Science and Engineering, Duke University Aug 2009-Present

• Assist with conceiving of and setting up events and programs to improve the climate for women in science and engineering.

Representative, The life society, Fudan University Oct 2005-May 2008

• Provided students who have career development concerns with different sources

• Promoted and popularized knowledge of life science disciplines, email update of the life society activities and organized posters.

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