SHARADH KUMAR

**** ******** ******,************ ** 19104

Email: y3btha@r.postjobfree.com

Phone: 267-***-****

Career Objective:

Biomedical engineering graduate student seeking a full time work position from June,2012.

EDUCATION

Masters in Biomedical Engineering in Drexel University, 2010-2012 (expected)

Relevant: Medical sciences, Molecular Biology, Genetic engineering, Medical Imaging systems, Biochemistry and Biostatistics,Medical device development

GPA-3.4

Bachelors of Technology in Biotechnology, Sastra University, India, 2006-2010

GPA-3.5

Relevant:Genetics,Biochemistry,Statistics,Microbiology,Cell biology,Engineering design

Thesis project internship at Sankara Nethralaya Hospital

WORK EXPERIENCE

Research Assistant, Department of Hematology, Children’s Hospital of Philadelphia, Philadephia, PA October 2011-Present

Working in a Project involving Intracellular Processing of Factor 8 in Platelet studies (mouse bone marrow transplantations and flow cytometric analyssis).

Cell isolation from bone marrow extracts,Cell culture,Platelets isolation,Perform ELISA

Assays,Perform Apoptosis Assays, Maintenance of animal colony,Genotyping

assays,Cell culture room maintenance.

Research Technician, Stress Neuroscience Department, Children’s Hospital of Philadelphia, Philadephia, PA, November 2010-June 2011

MRI Rat brain image analysis in the neuronal brain circuit inhibition by Manganese is monitored by conducting statistical analysis to measure the significance of stress response on incorporating manganese in rat brains.

Aided senior technicians in performing brain perfusions for detection of specific brain regions.

Statistical analysis to test for significance of data in correlation of brain regions in rats accounting for stress.

Research Internship, Microbiology Department, Sankara Nethralaya Hospital, India, January 2010-June 2010

Completed undergraduate thesis project ‘Detection of Isoniazid resistance in M.Tuberculosis’ using PCR and DNA sequence analysis to identify mutations within patient samples.

SKILLS AND TECHNIQUES

Laboratory: Cell culturing, ELISA,FACS, western blotting,DNA extraction, Agarose gel electrophoresis, brain perfusions, PCR, protein assay, immunohistochemistry, immunoelectrophoresis, animal handling, HPLC, hydrogel preparation and layer by layer coating, Kirby Bauer antibody method, UV-Spectrophotometer

Software and Programming: MS Office, SAS,SPSS,C,C++, Matlab and JMP,

HONORS AND AWARDS

Secured top grades in Genetics, Immunology courses in undergraduate level.

Organized National Biotech Entrepreneurship event VENSPIRE IN 2009 in Undergraduate College.

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