SHARADH KUMAR
**** ******** ******,************ ** 19104
Email: ************@*****.***
Phone: 267-***-****
Career Objective:
Biomedical engineering graduate student seeking a full time work position from June,2012.
EDUCATION
Masters in Biomedical Engineering in Drexel University, 2010-2012 (expected)
Relevant: Medical sciences, Molecular Biology, Genetic engineering, Medical Imaging systems, Biochemistry and Biostatistics,Medical device development
GPA-3.4
Bachelors of Technology in Biotechnology, Sastra University, India, 2006-2010
GPA-3.5
Relevant:Genetics,Biochemistry,Statistics,Microbiology,Cell biology,Engineering design
Thesis project internship at Sankara Nethralaya Hospital
WORK EXPERIENCE
Research Assistant, Department of Hematology, Children’s Hospital of Philadelphia, Philadephia, PA October 2011-Present
Working in a Project involving Intracellular Processing of Factor 8 in Platelet studies (mouse bone marrow transplantations and flow cytometric analyssis).
Cell isolation from bone marrow extracts,Cell culture,Platelets isolation,Perform ELISA
Assays,Perform Apoptosis Assays, Maintenance of animal colony,Genotyping
assays,Cell culture room maintenance.
Research Technician, Stress Neuroscience Department, Children’s Hospital of Philadelphia, Philadephia, PA, November 2010-June 2011
MRI Rat brain image analysis in the neuronal brain circuit inhibition by Manganese is monitored by conducting statistical analysis to measure the significance of stress response on incorporating manganese in rat brains.
Aided senior technicians in performing brain perfusions for detection of specific brain regions.
Statistical analysis to test for significance of data in correlation of brain regions in rats accounting for stress.
Research Internship, Microbiology Department, Sankara Nethralaya Hospital, India, January 2010-June 2010
Completed undergraduate thesis project ‘Detection of Isoniazid resistance in M.Tuberculosis’ using PCR and DNA sequence analysis to identify mutations within patient samples.
SKILLS AND TECHNIQUES
Laboratory: Cell culturing, ELISA,FACS, western blotting,DNA extraction, Agarose gel electrophoresis, brain perfusions, PCR, protein assay, immunohistochemistry, immunoelectrophoresis, animal handling, HPLC, hydrogel preparation and layer by layer coating, Kirby Bauer antibody method, UV-Spectrophotometer
Software and Programming: MS Office, SAS,SPSS,C,C++, Matlab and JMP,
HONORS AND AWARDS
Secured top grades in Genetics, Immunology courses in undergraduate level.
Organized National Biotech Entrepreneurship event VENSPIRE IN 2009 in Undergraduate College.