Medical Development
Resume:
Curriculum Vitae
Jean-Pierre L. Revelli, Ph.D.
** ********* *****, *** *********, Texas 77384
H. 713-***-****, C. 281-***-****, ********@*****.***
SUMMARY
• 999
• Broad genomics, molecular biology, biochemistry, and physiology experience from creating and analyzing animal model in support of drug development.
• Participate in strategic planning for program advancement.
• Excellent organizational, interpersonal and presentation skills; extensive experience in mentoring peer scientists.
RESEARCH EXPERIENCE
Oct.2011-Present
Adjunct Instructor of Biology
Lone Star College System
Biology Department
The Woodlands, TX 77384
2005- Feb.2011
Sr. Scientist
Department of Metabolism Research
Lexicon Pharmaceuticals, Inc.
The Woodlands, TX 77381
Reporting directly to the V.P. of the Metabolism department, I planned and conducted experiments using various techniques such as measurement of energy expenditure/consumption using indirect calorimetry, evaluation of gross motor activity and circadian rhythms for program progression. Managed metabolism programs from prioritization through the generation of experimental data and presented the results at jointpartner meetings with our pharmaceutical collaborators.
• Directly contributed to metabolism programs in support of drug pharmacology and development, including:
o mechanism of action of novel anti-obesity and diabetic drugs.
o proof of concept, mode of action, lead optimization, and clinical candidate selection.
• Identified and advanced 5 projects to advanced research programs.
• Develop project prioritization by analyzing genotype-phenotype correlation of over 50 novel genes.
• Conducted routine endocrinology assays, body composition analysis using DEXA and QMR techniques, oGTT, insulin ELISA, and mouse and rat compound dosing.
• Created rational het/het or het/het/het phenotypes for testing the hypothesis that partial inhibition of multiple kinases in the same pathway can produce a valued biologic response with potentially less toxicity.
• Established hyperinsulinemic euglycemic clamp in conscious mice.
1999-2005 Sr. Scientific Group Leader
Molecular Genetics Department
Lexicon Genetics, Inc.
The Woodlands, TX 77381
As Scientific Group Leader I participate in building and management of the Molecular Genetics Department, the only revenue generating department in the company. I supervised and managed curation of over hundreds knockout mouse production programs from three scientific team leaders. The efforts of my group helped Lexicon establish one of the largest collections of custom mouse mutations for pharmaceutically relevant drug targets in the world.
• Custom designed mouse mutations in an effort to evaluate the biotherapeutic value of a large array of genes potentially important as drug targets.
• Completed and delivered to alliance partners projects with knockout mice, within tight timelines.
• As a member of the drug discovery research committee, participated in drug target prioritization by mining genomic databases and curating over fifty projects for target validation by knockout mouse phenotype analysis.
• Designed new targeting vector technology and a new positive/negative selection strategy
1995-1999 Senior Postdoctoral Fellow
Department of Biochemistry
Baylor College of Medicine, Houston, TX 77030
Principal investigator: Professor G. Eichele
• Investigated pattern formation in limb development using chicken embryo as model.
• Investigated the ability of BMP-2 to direct limb patterning by creating zone of polarization (ZPA)-free wing buds.
• Investigated the role of nuclear receptor Ear2 in nervous system development, nociception, and forebrain clock function with Ear2-knockout mice.
1993-1995 Postdoctoral Fellow
Department of Medical Biochemistry, CMU
University of Geneva, Switzerland
Postdoctoral advisor: Professor J-P Giacobino
• Investigated the role of beta3 adrenergic receptor (Adrb3) function in energy balance, body fat accumulation, diet, and feeding behavior with Adrb3-knockout mice.
1989-1993 Graduate research assistant
Department of Medical Biochemistry, CMU
University of Geneva, Switzerland
Thesis mentor: Professor J-P Giacobino
Thesis title: “Molecular characterization of Beta3-adrenergic receptor in mouse”
• Cloned a novel beta-adrenergic receptor, Adrb3, from rat brown adipose tissue, where it controls thermogenesis and lipolysis.
• Investigated the involvement of Adbr3 in the pathogenesis of obesity
TECHNICAL SKILLS
Genomic long range PCR, targeting vector technology, mRNA purification, cDNA cloning, RT-PCR, DNA sequence analysis, in situ and whole mount hybridization, digital image analysis, embryonic stem cell technology, embryo manipulation and transplantation, microsurgery techniques, adenovirus gene delivery, post-operative animal care and maintenance. GPCR radioligand binding assays.
Computer technology: familiar with UNIX, Macintosh, IBM PC operating system, Software/Database manipulation including, NCBI sequences analysis software, Ensemble, BLAT, SMART, Procomp, GCG sequence analysis software, Sequencher, Adobe Photoshop and Illustrator.
EDUCATION
1989- 93 Ph.D. in Endocrinology
University of Geneva, Switzerland
Department of Medical Biochemistry
• Molecular characterization of Beta-3 adrenergic receptor of adipose tissue
1987- 89 Master in Molecular and Cellular Endocrinology
Université Pierre and Marie Curie, Paris VI
CNRS, CEREMOD, Meudon-Bellevue, France
• Molecular characterization of mitochondrial transporters in brown adipose tissue
• Fetal glucose utilization studies in rat
1986- 87 Bachelor of Science
Université Pierre and Marie Curie, Paris VI, France
TEACHING EXPERIENCE
1989-95 Teaching assistant,
Medical School, University of Geneva, Switzerland
• Metabolism, Molecular Biology: practical courses.
PEER-REVIEWED PUBLICATIONS
Profound Obesity Secondary to Hyperphagia in Mice Lacking Kinase Suppressor of Ras 2.
Revelli JP, Smith D, Allen J, Jeter-Jones S, Shadoan MK, Desai U, Schneider M, van Sligtenhorst I, Kirkpatrick L, Platt KA, Suwanichkul A, Savelieva K, Gerhardt B, Mitchell J, Syrewicz J, Zambrowicz B, Hamman BD, Vogel P, Powell DR.
Obesity (Silver Spring). 2010 Dec 2. [Epub ahead of print]
High-throughput screening of mouse knockout lines identifies true lean and obese phenotypes.
Brommage R*, Desai U*, Revelli JP*, Donoviel DB, Fontenot GK, Dacosta CM, Smith DD, Kirkpatrick LL, Coker KJ, Donoviel MS, Eberhart DE, Holt KH, Kelly MR, Paradee WJ, Philips AV, Platt KA, Suwanichkul A, Hansen GM, Sands AT, Zambrowicz BP, Powell DR.
Obesity (Silver Spring). 2008 Oct;16(10):2362-7.
*Authors contributed equally to the work.
Abnormal development of the locus coeruleus in Ear2(Nr2f6)-deficient mice impairs the functionality of the forebrain clock and affects nociception.
Warnecke M, Oster H, Revelli JP, Alvarez-Bolado G, Eichele G.
Genes Dev. 2005 Mar 1;19(5):614-25.
BACE (beta-secretase) modulates the processing of APLP2 in vivo.
Pastorino L, Ikin AF, Lamprianou S, Vacaresse N, Revelli JP, Platt K, Paganetti P, Mathews PM, Harroch S, Buxbaum JD.
Mol Cell Neurosci. 2004 Apr;25(4):642-9.
Efficient ectopic gene expression targeting chick mesoderm.
Oberg KC, Pira CU, Revelli JP, Ratz B, Aguilar-Cordova E, Eichele G.
Dev Dyn. 2002 Jul;224(3):291-302.
Expression of chick Tbx-2, Tbx-3, and Tbx-5 genes during early heart development: evidence for BMP2 induction of Tbx2.
Yamada M, Revelli JP, Eichele G, Barron M, Schwartz RJ.
Dev Biol. 2000 Dec 1;228(1):95-105.
Metabolic response to various beta-adrenoceptor agonists in beta3-adrenoceptor knockout mice: evidence for a new beta-adrenergic receptor in brown adipose tissue.
Preitner F, Muzzin P, Revelli JP, Seydoux J, Galitzky J, Berlan M, Lafontan M, Giacobino JP.
Br J Pharmacol. 1998 Aug;124(8):1684-8.
(-)-CGP 12177 causes cardiostimulation and binds to cardiac putative beta 4-adrenoceptors in both wild-type and beta 3-adrenoceptor knockout mice.
Kaumann AJ, Preitner F, Sarsero D, Molenaar P, Revelli JP, Giacobino JP.
Mol Pharmacol. 1998 Apr;53(4):670-5.
Targeted gene disruption reveals a leptin-independent role for the mouse beta3-adrenoceptor in the regulation of body composition.
Revelli JP, Preitner F, Samec S, Muniesa P, Kuehne F, Boss O, Vassalli JD, Dulloo A, Seydoux J, Giacobino JP, Huarte J, Ody C.
J Clin Invest. 1997 Sep 1;100(5):1098-106.
Retinoid signaling is required for the establishment of a ZPA and for the expression of Hoxb-8, a mediator of ZPA formation.
Lu HC, Revelli JP, Goering L, Thaller C, Eichele G.
Development. 1997 May;124(9):1643-51.
Respective degree of expression of beta 1-, beta 2- and beta 3-adrenoceptors in human brown and white adipose tissues.
Deng C, Paoloni-Giacobino A, Kuehne F, Boss O, Revelli JP, Moinat M, Cawthorne MA, Muzzin P, Giacobino JP.
Br J Pharmacol. 1996 Jun;118(4):929-34.
Control of beta 3-adrenergic receptor gene expression in brown adipocytes in culture.
Klaus S, Muzzin P, Revelli JP, Cawthorne MA, Giacobino JP, Ricquier D.
Mol Cell Endocrinol. 1995 Apr 1;109(2):189-95.
Characterization of a new, highly specific, beta 3-adrenergic receptor radioligand, [3H]SB 206606.
Muzzin P, Boss O, Mathis N, Revelli JP, Giacobino JP, Willcocks K, Badman GT, Cantello BC, Hindley RM, Cawthorne MA.
Mol Pharmacol. 1994 Aug;46(2):357-63.
Expression of beta 1- and beta 3-adrenergic-receptor messages and adenylate cyclase beta-adrenergic response in bovine perirenal adipose tissue during its transformation from brown into white fat.
Casteilla L, Muzzin P, Revelli JP, Ricquier D, Giacobino JP.
Biochem J. 1994 Jan 1;297 ( Pt 1):93-7.
Adipose tissues from various anatomical sites are characterized by different patterns of gene expression and regulation.
Cousin B, Casteilla L, Dani C, Muzzin P, Revelli JP, Penicaud L.
Biochem J. 1993 Jun 15;292 ( Pt 3):873-6.
Expression of the beta 3-adrenergic receptor in human white adipose tissue.
Revelli JP, Muzzin P, Paoloni A, Moinat M, Giacobino JP.
J Mol Endocrinol. 1993 Apr;10(2):193-7.
Modulation in vivo of beta-adrenergic-receptor subtypes in rat brown adipose tissue by the thermogenic agonist Ro 16-8714.
Revelli JP, Muzzin P, Giacobino JP.
Biochem J. 1992 Sep 15;286 ( Pt 3):743-6.
Progesterone transcriptionally regulates the beta 2-adrenergic receptor gene in pregnant rat myometrium.
Vivat V, Cohen-Tannoudji J, Revelli JP, Muzzin P, Giacobino JP, Maltier JP, Legrand C.
J Biol Chem. 1992 Apr 25;267(12):7975-8.
Radioligand binding studies of the atypical beta 3-adrenergic receptor in rat brown adipose tissue using [3H]CGP 12177.
Muzzin P, Revelli JP, Fraser CM, Giacobino JP.
FEBS Lett. 1992 Feb 24;298(2-3):162-4.
An adipose tissue-specific beta-adrenergic receptor. Molecular cloning and down-regulation in obesity.
Muzzin P, Revelli JP, Kuhne F, Gocayne JD, McCombie WR, Venter JC, Giacobino JP, Fraser CM.
J Biol Chem. 1991 Dec 15;266(35):24053-8.
Changes in beta 1- and beta 2-adrenergic receptor mRNA levels in brown adipose tissue and heart of hypothyroid rats.
Revelli JP, Pescini R, Muzzin P, Seydoux J, Fitzgerald MG, Fraser CM, Giacobino JP.
Biochem J. 1991 Aug 1;277 ( Pt 3):625-9.
The novel thermogenic beta-adrenergic agonist Ro 16-8714 increases the interscapular brown-fat beta-receptor-adenylate cyclase and the uncoupling-protein mRNA level in obese (fa/fa) Zucker rats.
Muzzin P, Revelli JP, Ricquier D, Meier MK, Assimacopoulos-Jeannet F, Giacobino JP.
Biochem J. 1989 Aug 1;261(3):721-4.
Fetal glucose utilization in response to maternal starvation and acute hyperketonemia.
Leturque A, Hauguel S, Revelli JP, Burnol AF, Kandé J, Girard J.
Am J Physiol. 1989 Jun;256(6 Pt 1):E699-703.
Hyperglycemia and hyperinsulinemia increase glucose utilization in fetal rat tissues.
Leturque A, Revelli JP, Hauguel S, Kande J, Girard J.
Am J Physiol. 1987 Dec;253(6 Pt 1):E616-20.
BOOK CHAPTER
Ricquier D., Bouillaud F., Villaroya F., Champigny O., Hentz E., Rainbault S., Casteilla L., Mory G., and Revelli J-P.
Control of uncoupling protein gene expression in rodents and humans
In: Highlights on Endocrinology, Christiansen C, & Riis B.J. (Eds) (1989)
pp. 367-371, Morhaven Botrykkeri, Copenhagen.
ABSTRACTS
Evidence for positional signaling by BMP-2 in the chick limb bud
Revelli J-P., Thaller C., and Eichele G.
Dev. Biol. (1998), 198, p 184.
A patterning role of BMP-2 in the vertebrate wing
Revelli J-P., Thaller C., and Eichele G.
Baylor Biochemistry Research Conference, 1997
Expression of Beta3-adrenergic receptor in human white adipose tissue
Revelli J-P., Muzzin P., Paoloni A. Moinat M., khune F., and Giacobino J-P.
International Obesity Conference, Geneva 1992/
Int. J. Obesity (1991), 17, Suppl. 1, pS83.
Molecular characterization of an atypical rat Beta-adrenergic receptor
Revelli J-P., Muzzin P., Gocayne J., Fraser C.M., Venter J. C., and Giacobino J-P.
3rd European Congress on Obesity EASO, Nice 1991.
Int. J. Obesity (1991), 15, Suppl. 1, pS73.
Changes in Beta1 and Beta2-adrenergic receptor mRNA levels in brown adipose and heart of hypothyroid rats
Revelli J-P., Pescini R., Muzzin P., Seydoux J., Fitzgerald M.G., Fraser
C.M., and Giacobino J-P.
23th Annual USGEB Meeting, Geneva 1991.
Experimentia (1991) 47, pA24.
FELLOWSHIPS AND AWARDS
1995-1996 Young scientist fellowship, Swiss National Research Foundation
1996-1997 Advanced scientist fellowship, Swiss National Research Foundation
2004 Lexicon Innovation Award
“Implementation of Phusion DNA Polymerase Long Range PCR” Targeting vector engineering
Molecular Genetics Recognition Award
“100th line of KO mice achieving germline transmission”
REFERENCES
David R. Powell, MD
Vice President Metabolism
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
The Woodlands Texas 77381
T. 281-***-****
*******@*********.***
www.lexpharma.com
Robert Brommage PhD
Director Metabolism
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
The Woodlands Texas 77381
T. 281-***-****
*********@*********.***
www.lexpharma.com
Zhi-Ming Ding, MD PhD
Director Cardiology
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
The Woodlands Texas 77381
*****@*********.***
www.lexpharma.com
Melissa Yang, PhD
Principal Clinical Scientist
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
The Woodlands Texas 77381
*****@*********.***
www.lexpharma.com
Christophe Person
VP of Operations and Information Systems
Lexicon Pharmaceuticals Inc.
8800 Technology Forest Place
The Woodlands Texas 77381
T. 281-***-****
*******@*********.***
www.lexpharma.com
Jean-Paul Giacobino, MD
Ph.D. thesis mentor
Centre Medical Universitaire
Departement de Biochimie
1 rue Michel Servet
1211 Genève 4
*********@*****.***
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