Scientist, Emerging Infectious Diseases
Resume:
Curriculum Vitae
Claire Heather Wernly, Ph.D.
*******@*****.***
Education:
**** ****** ** ********** ** Emerging Infectious Diseases
Uniformed Services University of the Health Sciences
F. Edward Hebert School of Medicine
Bethesda, MD.
2002 FAES National Institutes of Health
Basic Principles of Immunology and Hypersensitivity
Bethesda, MD.
2001 Bachelor of Science in Microbiology
Citation in German Studies
University of Maryland
College Park, MD.
2000 Eberhard Karls Universität
Tübingen, Germany
Work Experience:
2010-present
Sanaria, Inc.
Scientist
2003-2010
Graduate Student/Research Assistant
Uniformed Services University of the Health Sciences
2001- 2002
National Institutes of Health/ NIAID
Microbiologist
Awards & Honors:
2003-2010 Uniformed Services University Graduate Studies Stipend
2002 NIAID Staff Recognition Award
2000 3rd Place Winner, U. of MD Technical Writing Competition
1997 University of Maryland, Director's Scholarship for Music
Publications:
1. Lai, C-J., A.P. Goncalvez, R. Men, C. Wernly, O. Donau, R.E. Engle and R.H. Purcell. 2007. Epitope Determinants of a Chimpanzee Dengue Virus Type 4 (DENV-4) - Neutralizing Antibody and Protection against DENV-4 Challenge in Mice and Rhesus Monkeys by Passively Transferred Humanized Antibody.
J. Virol. 81: 127**-*****.
2. Goncalvez, A.P., R. Men, C. Wernly, R.H. Purcell and C-J Lai. 2004. Chimpanzee Fab Fragments and a Derived Humanized Immunoglobulin G1 Antibody That Efficiently Cross-Neutralize Dengue Type 1 and Type 2 Viruses. J. Virol. 78: 129**-*****.
3. Men, R., T. Yamashiro, A.P. Goncalvez, C. Wernly, D.J. Schofield, S.U. Emerson, R.H. Purcell and C-J Lai. 2004. Identification of Chimpanzee Fab Fragments by Repertoire Cloning and Production of a Full-Length Humanized Immunoglobulin G1 Antibody That is Highly Efficient for Neutralization of Dengue Type 4 Virus. J. Virol. 78: 4665-4674.
Abstracts and Thesis:
1. Wernly, C.H. 2010. Evolution of HIV-1 Envelope glycoprotein genes and the neutralizing antibody response in an individual whose plasma contains broadly neutralizing antibodies. Ph.D. Dissertation. Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD.
2. Wernly, C.H., P. Zhang, Z. Zhang, G.V. Quinnan. 2009. Evolution of HIV-1 Envelope Glycoprotein (Env) Gene in an Individual with Broadly Cross-Reactive Neutralizing Antibodies. Poster. Keystone Symposia, Keystone CO.
3. Wernly, C.H., P. Zhang, Z.Zhang, G.V. Quinnan. 2007. Evolution of the HIV-1 envelope against the host immune response from a Long-term Nonprogressor over the course of 21-year sample availability. Poster. Research Week. Uniformed Services University of the Health Sciences, Bethesda, MD.
4. Wernly, C.H., G.V. Quinnan. 2006. Replacement of the carboxy termini of Hepatitis C Virus envelope glycoproteins with alternative transmembrane domain segments from mammalian proteins results in the expression of viral antigen of the cell surface. Poster. Research Week. Uniformed Services of the Health Sciences, Bethesda, MD.
Seminars:
1. “Evolution of the HIV-1 Envelope Glycoprotein Genes and Neutralizing Antibody Response in an Individual with Broadly Cross Neutralizing Antibodies.” 2010. Doctoral Defense Seminar. Uniformed Services University of the Health Sciences, Bethesda, MD.
2. “Evolution of the HIV-1 Envelope Glycoprotein Gene in an Individual with Broadly Cross-Reactive Neutralizing Antibodies.” 2008. Senior Student Seminar. Uniformed Services University of the Health Sciences, Bethesda, MD.
3. “Exploring New Ways to Improve the Immunogenicity of HCV and HIV-1 in order to Induce Potent Neutralizing Antibodies.” 2007. Senior Student Seminar. Uniformed Services University of the Health Sciences, Bethesda, MD.
Research Experience:
Doctoral Research (2003-2010)
Research was performed in the laboratory of Gerald V. Quinnan, Jr., M.D. at the Uniformed Services University, Bethesda, MD.
Studied the evolution of HIV-1 envelope glycoprotein genes and neutralizing antibody response in an individual with broadly cross neutralizing antibodies. Work resulted in a doctoral thesis, a paper “in preparation” and three abstracts for posters. Posters were presented at the annual Research Week conducted at the Uniformed Services University as well as at the Keystone Symposium, Keystone CO.
Laboratory techniques include: BSL-2 and 3 conditions, generation of live HIV-1 viral stocks, manipulation of HIV-1 infected human blood, plasma and cells, isolation of genomic DNA, PCR amplification of proviral DNA, molecular cloning, screening, generation of pseudoviruses for use in neutralization assays, luminescence, gel electrophoresis, western blot, ELISA, PCR, sequencing, mutagenesis and statistical analysis.
Additional side research project focused on the replacement of the carboxy termini of HCV envelope glycoproteins with alternative transmembrane domain segments from mammalian proteins resulting in the expression of viral antigen on the cellular surface.
Assisted in the immunization and bleeding of mice and rabbits for various experiments aimed at determining the efficacy of alphavirus vector vaccine candidates for HIV-1.
National Institutes of Health
Microbiologist (2001-2003)
Research was performed in the laboratory of Ching-Juh Lai, Ph.D. at NIH/NIAID.
Isolated neutralizing Fab fragments from the bone marrow of Chimpanzees infected with Dengue Viruses 1-4. Work resulted in three publications in the Journal of Virology.
Laboratory techniques included: Genomic DNA isolation, generation of cDNA libraries, phage panning, molecular cloning into bacterial and mammalian expression vectors, protein expression in E.coli and CHO cells, screening of bacterial clones, maintenance of bacterial stocks, maintenance of mammalian cell lines, and maintenance of viral stocks, virus neutralization assays, plaque assays, generation and characterization of neutralization escape mutants, microscopy, immunostaining, gel electrophoresis, western blot, ELISA, PCR, and sequencing. Additional responsibilities included basic laboratory maintenance and procurement.
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