Chemist/ Research Associate/QA/QC/Analytical

KEYURI SHAH

xc5h9e@r.postjobfree.com **C McGreevey Way, Boston, MA 02120 508-***-****

EDUCATION:

Northeastern University, Boston, MA September 2011

Master of Science in Biotechnology

Rajiv Gandhi University of Health and Sciences, Bangalore, India October 2008

Bachelor of Science in Pharmacy

WORK EXPERIENCE:

Northeastern University- Barnett Institute of Chemical and Biological Analysis June 2011 - Present

Intern

• Maintain and review SOPs

• Perform literature search on GLP and GMP practices

• Assist in developing Analytical Validation and Transfer Protocol

• Assist in establishing a GLP quality system for Center for Advanced Regulatory Analysis

• Obtain basic understanding and knowledge of GLP, cGMP, FDA and ICH guidelines

• Following good documentation practices

Northeastern University - Center of Pharmaceutical Sciences September 2010 –May 2011

Graduate Research Assistant

• Grown Arabidopsis thaliana under artificial conditions using aerogardens under different stress conditions

• Examined effects of stress conditions microscopically and physically

• Assisted in analyzing binding sites in plant by performing cell binding assays for acyl ethanolamine using tritiated cocaine

• Assisted in in-vivo experiments to prepare tissue samples for calculating the activity of tritiated cocaine with radio-ligand compounds

• Assisted in running RP- HPLC and semi-automatic cell harvester

• Documented the results

Lincoln Pharmaceuticals Ltd., Gujarat, India December 2008-February 2009

Intern

• Assisted in formulating tablets for anti-psychotic drugs by optimizing all parameters in cGMP environment

• Performed Quality Control tests for tablets mainly dissolution and disintegration tests

• Obtained basic understanding of liquid and capsule formulation

ACADEMIC LABORATORY EXPERIENCE:

Biochemical Toxicology Laboratory Course January 2011-April 2011

• Measured Cytochrome P450 induction by Phenobarbital exposure and subcellular distribution of drug metabolizing activity by differential centrifugation

• Evaluated Acetaminophen induced hepatoxicity in presence of Pyridine and N-Acetyl Cysteine

• Examined effects of Carbon tetrachloride induced lipid peroxidation against α-tocopherol

• Conducted comparative acute toxicity study of Sodium Cyanide and Acrylonitrile

• Performed BUN assay to determine nephrotoxicity induced by Mercuric Chloride

• Performed Colorimetric Assay to determine effect of organophosphate insecticides and antidotes on brain Acetyl cholinesterase activity

Instrumentation: UV-VIS Spectrophotometer, Surgical instruments (scissors, scoop, blunt forceps), centrifuge

Pharmaceutical Science Laboratory Course August 2010-December 2010

• Maintained NIH3T3 cells and transfected the cells with pEGFP

• Determined the protein concentration of the transfected cells with help of BCA assay and ELISA.

• Performed SDS-PAGE to determine the molecular weight of the proteins present in NIH3T3 cells, and specific protein β actin was determined using Western Blot.

• Performed Cytotoxicity Assay to determine the toxicity of a drug on NIH3T3 cells

• Isolated IgG using Affinity Chromatography

• Performed restriction digestion of pEGFP- N1 by Not1 and Xho1 restriction endonucleases along with visualization of the DNA bands by gel electrophoresis and Gel Extraction of Xho1 linearized plasmid DNA

• Extracted and quantified RNA to synthesize cDNA and amplified it using PCR

Instrumentation: UV-VIS Spectrophotometer, HPLC, Autoclave, Fluorescent Microscope, Nano Drop Spectrophotometer, Plate reader, pH meter, Freeze dryer, Coulter Counter Particle Size Analyzer, Zeta Potential Analyzer

Introductory Biotechnology Laboratory Course January 2010 - April 2010

• Estimated the protein concentration in the samples using BCA and Bradford Assay

• Performed SDS-PAGE and Native PAGE for determining the molecular weight of the proteins present in the sample

• Performed the sandwich ELISA for these samples to determine the total concentration of the IgG in the samples

• Learnt the basics of HPLC and Mass Spectrophotometry

Instrumentation: UV-VIS Spectrophotometer, HPLC, pH meter, Plate reader, Mass Spectrophotometer

COMPUTATION KNOWLEDGE:

• Biometrics (SPSS)

• Microsoft applications (Excel, Word, Power point)

RELEVANT COURSEWORK:

Pharmaceutical Laboratory, Biotechnology Laboratory, Biochemical Toxicology Laboratory, Advanced Biochemistry, Molecular cell Biology, Advanced Drug Delivery Systems, Drug Discovery and Design, Biometrics and Statistics, Concepts in Pharmaceutical Science, Pharmacokinetics and Drug Metabolism, Human Physiology and Pathophysiology

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