Manager Medical

RAYMOND W. WASHINGTON

**** **** ****, *******, ** 48918 • Tel: 517-***-**** • *********@*******.***

OBJECTIVE

To secure a position in a reputable company/institution where my vast scientific knowledge and experience can contribute to its productivity.

SUMMARY

Highly qualified, versatile Research Scientist with over 15 years experience and professional strengths that include:

• Focused and detail orientated

• Work productively both independently and in teams

• Ability to identify most important concepts, information and to troubleshoot

• Creative, analytical, effectively manage time and multiple projects, set priorities, meet deadlines and supervise

• Work well under pressure

• Very good bench skills

• Learns very quickly

• Very comfortable teaching in the classroom/laboratory

• Able to explain concepts very clearly

• very approachable and concerned with student learning

TECHNICAL SKILLS

Proficient in:

• Hybridoma Production, Antibody Characterization and Purification, Protein Purification, Size Exclusion, Affinity, and Ion Exchange Column Chromatography

• Mammalian Cell Transfection, Mammalian Cell Tissue Culture, Primary Stem Cell Isolation and Culturing, Mammalian Primary Cell Tissue Culture, Suspension CHO Cell Transfection, Selection, Growth Optimization, Sub cloning and Cell Culture, Dictyostelium Cell Culture, Various Culture Media Preparations

• Frozen and Paraffin Embedded Tissue Block Preparation and Sectioning

• Immunohistochemistry, Immunocytochemistry, Flow Cytometry, ELISA Design and implementation, Micro plate Fluorescent and Cell Based Assays

• Light, Confocal, Fluorescent, Electron Scanning and Transmission Microscopy

• Primer Design, PCR, Plasmid Construction and Fusion Protein Expression, Protein (PAGE) and DNA Electrophoresis, Western Blot and IEF.

• Small Animal Handling, Surgery and Genotyping

• Adhesion, Migration, Invasion and Chemotaxis Assays

• Familiar With: siRNA Technology, RT-PCR, qRT-PCR and Cell Signaling

SOFTWARE/SYSTEM SKILLS

Experienced on Mac and PC platforms

• Microsoft Word, Excel, and PowerPoint.

• DNA program: Vector NTI

• Graphing/Statistical Program: Prism

• Graphics/Imaging Programs: Canvas, NIH ImageJ, Photoshop

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EXPERIENCE

Research Associate

Michigan State University/ School of Human Medicine/Department of Surgery East Lansing, MI 48824

• Initial lab set-up and maintenance

• Tissue culture of human pancreatic cancer cell lines

• Isolation, Identification and primary tissue culture of human pancreatic cancer stem cells

• Independent experimental design, completion, analysis and troubleshooting

• Cell proliferation assay of human pancreatic cancer cell lines after drug treatment

• Protein PAGE and western blot

• Training and supervision of undergraduate students

• Collaboration with multiple laboratories

• Lab Manager (functioning on Assistant Professor level)

• Record keeping and weekly reporting to superiors

Cell Line Development Scientist

Millipore Corporation/ Technology and Pre-Development/Expression Technologies Bedford, MA 01730

2010 to 2010

Work involved the optimization and comparison of growth, of CHO Dg44 and CHO K-1, in different culture medium for use in a bioreactor. Included also was transfection, selection and culturing of transfected cells.

Research Fellow

Beth Israel Deaconess Medical Center/Harvard Medical School/Department of Vascular Biology and Angiogenesis Boston, MA 02215

2006 to 2010

Using human tissue and a murine model of human breast cancer to investigate the function of a matricellular protein, previously unreported, expressed in the tumor stoma of breast cancer.

• Immediately established protocol to separate and primary culture pure populations of Cancer Associated Fibroblasts and Breast Cancer Cells

• Definitively determined the Cancer Associated Fibroblasts to be the source matricellular protein expression

• Impacted colleagues research by supplying protocol to obtain pure cultures of tumor cells

• Established collaborations with other labs/departments that needed to utilize skills possessed

Graduate Student (New England Board of Higher Education Fellow)

University of Connecticut/Department of Molecular and Cell Biology/Cell Biology Storrs, CT 06269 1995 to 2006

Investigation of actin-binding proteins and cell motility in the Dictyostelium model system.

• Successfully built functional GFP and RFP fusion proteins allowing for real time visualization of protein localization

• Disproved previously held beliefs of functional redundancy of ABP-120 and alpha-Actinin by dual probe live cell imaging

• Introduced concept that actin filaments at the leading edge of motile cells may be different from cortical actin

Research Associate I

University of Michigan Medical School/Department of Surgery/Urology Ann Arbor, MI 48104

1987 to 1995

Production of hybridomas and monoclonal antibodies raised against fresh human bladder cancer.

• Produced and characterized an antibody subsequently sold to a pharmaceutical company

• Antibody being used as a diagnostic tool Today

PUBLICATIONS

1. Liebert M, Wedemeyer GA, Stein JA, Washington RW, Jr, Flint A, Ren L, Grossman HB:

Identification by monoclonal antibodies of an antigen shed by bladder cancer cells. Cancer Res

49:6720-2726, 1989.

2. Harney JV, Liebert M, Wedemeyer G, Washington R, Stein J, Bauchbaum D, Steplewski Z,

Grossman HB: The expression of epidermal growth factor receptor on human bladder cancer:

Potential use in radioimmunoscintigraphy. J Urol 146:227, 1991.

3. Grossman HB, Washington RW, Carey TE, Liebert M: Alterations in antigen expression in

superficial bladder cancer. J Cell Physiol, supp 161:63-68, 1992.

4. Liebert M, Wedemeyer G, Stein JA, Washington R, Faerber G, Flint A, Grossman HB: Evidence for

urothelial cell activation in interstitial cystitis. J Urol 149: 470-75, 1993.

5. Liebert M, Wedemeyer G, Stein JA, Washington R, Carey TE, Grossman HB: The Monoclonal

Antibody BQ16 identifies the alpha 6 beta 4 integring in bladder cancers. Hybridoma 12:67-80.

1993.

6. Liebert M, Washington R, Wedemeyer G, Carey TE, Grossman HB: Loss of co-localization of alpha

6 beta 4 integrin and collagen VII in bladder cancer. Am J Pathol 144(4):787-95 1994.

7. Liebert M, Washington R, Wedemeyer G, Stein J, Grossman HB: Expression of the VLA-B1

integrin family in bladder cancer. Amer J Pathol 144(5):1016-1022, 1994.

8. Tong X, Yin L, Washington R, Rosenberg DW, Giardina C: The p50-p50 NF-?B complex as a

stimulus-specific repressor of gene activation. Mol Cell Biochem. 265: 171-183, 2004.

9. Washington R, Knecht D: Actin binding domains direct actin-binding proteins to different

cytoskeletal locations. BMC Cell Bio. Feb. 13;9:10, 2008.

10. Yee KO, Connelly CM, Duquette M, Kazerounian S, Washington R, Lawler J: The effect of

thrombospondin-1 on breast cancer metastasis. Breast Cancer Res Treat. Apr 13 2008.

Chapters:

1. Grossman HB, Liebert M, Sakakibara N, Wedemeyer GA, Stein JA, Washington RW: "Evaluation

of a new bladder tumor marker." in: Urologic Clinics of North America. Volume 18. W. Fair, editor;

W.B. Saunders, Inc., Philadelphia, 1991.

EDUCATION

University of Connecticut

Molecular and Cell Biology/Cell Biology/Ph.D. Storrs, CT 06269

1995 to 2006

Thesis Title: The Role of the Actin-Binding Domain in the Intracellular Localization of ABP-120 and alpha-Actinin in Dictyostelium discoideum

Eastern Michigan University/University of Michigan

Biology/MS Ypsilanti, MI 48197

1991 to 1995

Thesis Title: DD23: a Monoclonal Antibody Specific for Breast Cancer

ADDITIONAL INFORMATION

Teaching Experiences:

2003 Biology Instructor for the University of Connecticut Health Center Department of Health Careers Opportunity Programs.

--Cell Biology (Summer of 2003 and 2004)

2002 Biology Instructor for The University of Connecticut Academic Center for Entering Students 3000 X 2000 Program.

--Introductory Biology (Summer of 2002)

Informal teaching activities:

1996-1998 Tutor for The University of Connecticut Counseling Program for Intercollegiate Athletics .

--Biochemistry, Cell Biology, Immunology

1996 Mentor for the National Institutes of Health NCRR High School Student Research Apprentice Program.

--Summers of 1996, 1997, 1998 and 2000

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