Medical Supervisor

DONNA THEE

Florida Licensed Clinical Laboratory Supervisor in Molecular Pathology, Microbiology, Serology

B.S., M (ASCP)CM, MB (ASCP)CM, RM (NRCM), ClSp(MB)

**** *. **** **** *****, B29-108 Phone: 954-***-****

Pompano Beach, FL 33069 E-Mail: *********@*****.***

A seasoned laboratory professional offering years of clinical, research and commercial microbiology and molecular laboratory experience.

A scientist dedicated to remaining current by attending national meetings, taking courses in new technologies and obtaining CME and CEU in addition to those required for licensure.

OBJECTIVE

To utilize my knowledge and experience to benefit patient care in an environment with dedicated team members that fosters continued growth.

HISTORY AND ACCOMPLISHMENTS

Memorial Healthcare System – Hollywood, Florida

Medical Technologist, Memorial Regional Hospital Microbiology, 7/06 to 7/12

High volume lab serving 5 hospitals plus clinics in South Broward Hospital District

Perform aerobic, anaerobic and microaerophilic cultures on all specimen sources, utilizing special media and conditions as appropriate

Accession and process specimens

Utilize BacTAlert blood culture and VitekII identification and susceptibility instruments

Perform manual bacterial testing: Kirby Bauer and E-test susceptibilities, Hodge test, API and Rapid ID (NF,ANA,Yeast), agglutination tests: Salmonella & Shigella, EcoliO157, Beta strep, Staph,

Prepare, read and interpret Gram, Acid Fast, Modified and Partial Acid Fast stains

Perform Ova & Parasite identification: processing specimen, reading direct and stained slides

Perform Rapid Influenza A&B, RSV and Clostridium difficile testing

Perform instrument, reagent, media, antibiotic quality control.

Troubleshoot instrument and assay problems

Assure specimen integrity and sample identification on specimens received and shipped

Adhere to policy and procedure, safety and regulatory requirements

Assure correct and timely reporting of results

Interact with physicians, nurses and other departments

Write and submit incident reports as necessary to improve processes

Charge tech 2nd shift

Quest Diagnostics, Inc. – Deerfield Beach, Florida

Medical Technologist II, Molecular Diagnostics Department, 9/03 to 6/06

Roche Amplicor Standard and Ultrasensitive HIV Quantitative RNA RNA Assay

GenProbe Aptima, Roche Cobas and BD Viper SDA assays for CT and GC.

Taqman Real time HCV RNA assay

Digene manual Hybrid Capture HPVassay

Digene HC2 HPV automated assay: set up and validated assay, lead tech

Maintain instruments, perform assay and instrument QC

Troubleshoot instrument and assay problems

University of Cincinnati - Cincinnati, Ohio

Jr. Research Associate, Infectious Diseases, Digestive Diseases 9/89 to 7/03

Technical Supervisor UIMA Retrovirology Reference Laboratory

Technical Supervisor UIMA Molecular Hepatology Laboratory

Genotyping: Open Gene HIV sequencing,Line Probe HCV typing

RNA, DNA, protein extraction; Northern, Southern, Western blots

Restrictive enzyme analysis, cloning bacterial transformation, probe preparation, In-situ hybridization, heteroduplex analysis, SSCP, RFLP, sequence editing, phylogenetic study

Tissue culture, HIV Culture, p24 antigen, PBMC and PMN isolation and cryopreservation

AIDS Clinical Trials Group protocol teams, vice chair technologist committee

Humana Hospital Cypress – Pompano Beach, Florida

Supervisor, Microbiology and Serology, 9/88 to 9/89

Broward General Medical Center – Fort Lauderdale, Florida

Senior Medical Technologist, 10/82 to 3/88

Microbiology Supervisor or Technologist and part time:

North Ridge Medical Center-Oakland Park, Florida

Roche Clinical Laboratory-Coral Gables, Florida

American Hospital-Miami, Florida

Skokie Valley Community Hospital-Skokie, Illinois

University of Cincinnati Medical Center-Cincinnati, Ohio

American Laboratory Associates-Fort Lauderdale, Florida

Broward 80 Medical Laboratory-Fort Lauderdale, Florida

EDUCATION

University of Cincinnati, Cincinnati, Ohio, B.S. Biology

Nova University, Davie, Florida, 26 semester hours graduate study Cell Biology

KNOWLEDGE AND SKILL MAINTENANCE

Attend annual American Society for Microbiology national meeting

Attend Association for Molecular Pathology annual meeting on Genomic Medicine

Attend 1-2 day workshops: Most recently: Trends in Clinical Laboratory Identification of Microbes in 2012:

MALDI ToF MS, June 2012

PROFESSIONAL LICENSURE AND AFFILIATIONS

Florida Licensed Clinical Laboratory Supervisor, Molecular Pathology, Microbiology and Serology (SU10330)

Technologist in Molecular Pathology, American Society for Clinical Pathology-MB(ASCP)CM (000100)

Technologist in Microbiology, American Society for Clinical Pathology-M(ASCP)CM (004179)

Registered Microbiologist, National Registry of Certified Microbiologists, American Academy of Microbiology-

RM(NRCM) (004368486)

Certified Laboratory Specialist in Molecular Biology, National Credentialing Agency for

Laboratory Personnel -CLSpMB (9911328) (now merged with ASCP)

Association for Molecular Pathology (16566)

American Society for Microbiology (4368486)

American Society for Clinical Pathology (04148016)

American Society for Clinical Laboratory Science (222601)

Aids Clinical Trials Group Laboratory Technologist Committee, Vice Chair 1998-2000

American Mensa Limited (1050370)

International Ryukyu Karate-jutsu Research Society,

Nidan (2nd degree black belt) Okinawan Shorin Ryu

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