Research Associate Professor

Keith Alvares, Ph.D

**** *. ********

Chicago, IL. 60659

Tel # 773-***-****(Home)

312-***-**** (Work)

Cell # 312-***-****

e-mail: *-*******@************.***

PROFESSIONAL SUMMARY:

A biochemist/molecular biologist with a strong background in gene isolation, and the expression and purification of proteins, from various systems. A strategic thinker with leadership skills.

AREAS OF SPECIALIZATION

• Expert knowledge of the expression and purification of proteins using bacteria, insect and mammalian cells.

• Generated permanent cell line expressing various different proteins

• Expert knowledge of the purification of proteins from biological materials

• Experience in protein-protein interactions

• Construction and screening of genomic and cDNA libraries using antibodies and cDNA probes

• Insitu hybridizations using cDNA’s and ologonucleotide probes

• Skilled in the use of computers for DNA, protein analysis and data bank searches

• Experience in the fields of genotoxic and non-genotoxic carcinogens

• Generated transgenic mice

• Experience in the field of extra cellular matrix and biomineralization

PROFESSIONAL EXPERIENCE

Research Associate Professor, Department of Cell and Molecular Biology, Northwestern University Medical School June2009- present

• Studying the mechanism of bio-mineralization in sea urchin and rat teeth. (J. Biol Chem. (2009) 284, 26149- 16160)

• Studying the effects of acidic proteins on the development of the kidney

Research Assistant Professor, Department of Cell and Molecular Biology, Northwestern University Medical School. June 1996-May2009:

• Studying the mechanism of chain selection in type 1 collagen, and the role played by non-collagenous proteins in tooth mineralization and tissue differentiation. Expressed the two chains of collagen in bacteria, insect and mammalian cells. Purified the expressed proteins and studied their interactions

• Co-Investigator; R01 AR 13921-40 Veis (PI) 3/01/02-2/28/05

NIH/NIAMSD. Structure and Assembly of Collagen Molecules and Fibrils: This project is to study the structure of the collagen molecule and the factors that guide both Intracellular assembly into the heterotrimeric molecule and the extracellular assembly into fibrils.

• Showed for the first time that the alpha 2 subunit of collagen is capable of binding to itself (Biochemistry (1999), 38(17), 5401-5411; Biochemistry (2005) 44(46); 152**-*****)

• Co- Investigator; R01 DE 01374-42 Veis (PI) 1/01/98-04/30/06

NIH/NIDR: Matrix Component Interactions in Bone and Dentin.

This project is focused on the non-collagenous proteins that participate in the induction and stabilization of the mineral phases in bone and dentin

• Expressed Dentine Phosphoprotein (DPP) in bacteria and fibroblasts, purified the expressed protein and showed for the first time that DPP, believed to be a dentine specific structural protein may be involved in kidney and lung embryonic development (Developmental Dynamics (2006) 235; 2980-2990)

• Isolated the gene for Mortalin from se urchin teeth, and showed that it is involved in synctium formation (J. Exp. Zoology Part B. Molecular and Developmental Evolution (2007) In Press

• Co-Investigator; R01 DE01478-01A1 Veis (PI) 05/01/03 – 04/30/07

NIH/NIDCR. Signaling Activities of Amelogenin Gene Splice Products.

The small amelogenin splice product peptides have been shown to have cell signaling activity. We are studying their specific role in epithelial-mesenchymal communication and interactions during tooth development. (J. Biol. Chem. (2000) 275, 412**-*****; J. Bone and. Mineral Research (2005) 20, 341-349)

• Mentored three graduate students and four postdoctoral

Research Assistant Professor, Department of Pathology, Northwestern University Medical School. Feb. 1993 - May 1996.

• Used differential and subtractive hybridization techniques to isolate genes that are differentially regulated by peroxisome proliferators, to gain insights into their role played in formation of hepatocellular carcinomas and showed that peroxisomal proliferators are also capable of turning off non-peroxisomal genes. (Carcinogenesis, (1996) 17(2), 311-316)

• Involved in the isolation of a peroxisome proliferators response element (Proc. Natl. Acad. Sci. (1992) 89, 7541-7545) and created transgenic mice under the control of this element (Cancer Research, (1994) 54, 2303-2306)

• Involved in the isolation of the gamma form of the peroxisomal proliferators activated receptor (PPARJ. Biol. Chem. (1993) 268, 268**-*****)

• Generated permanent cell lines expressing Hydrogen peroxide generating Fatty acyl-CoA oxidase . (Proc. Natl. Acad. Sci. (USA). (1995) 92, 7080-7084) and urate oxidase Biochem. Biophys. Res. Commun. (1994), 200, 178-186

• Mentored three graduate students and six postdoctoral

Researcher Associate, Department of Pathology Northwestern University Medical school, Feb.1988 - April 1993.

• Identified the peroxisome proliferators binding protein as a member of the HSP 70 protein family. (Proc. Natl Acad. Sci. (USA), (1990), 87, 5293-5297).

• Isolated the cDNA for rat liver urate oxidase. (Biochem. Biophys. Res. Commun. (I989) 158, 991-995), Expressed the cDNA in insect cells and showed that it is capable of forming crystals in vivo. (Proc. Natl. Acad Sci. (USA), (1992) 89, 4908-4912).

Mentored four postdoctoral fellows

Post doctoral Fellow, Department of Pathology, Northwestern University Medical School, Nov. 1985 – Feb. 1988.

• Isolated several peroxisomal genes, and expressed them in bacterial, insect and mammalian cells. Studied the pleiotropic effect of peroxisome proliferators

• Isolated a peroxisomal proliferator binding protein, (Proc. Natl. Acad. Sci. (USA), (1987) 84, 5342-5347)

ADDITIONAL EXPERIENCE

• Taught a course on “Toxicant effects mediated by steroid and other receptors” for the Continuing Education Program at the 1996 Society of Toxicology Meeting held at Anaheim CA.

• Presented data at several International conferences

• Reviewer for the Journal of Toxicology and Applied Pharmacology

• Reviewer for the Journal of Connective tissue Research

• Coordinated and managed research and funding efforts of the laboratory

• Developed and implemented regulated laboratory procedures

• Designed, implemented, evaluated, and documented the laboratories experiments

• Mentor to high school students of the Illinois Mathematics and Science Academy

EDUCATION:

Ph. D. Biochemistry, University of Madras, India,

M. Sc. in Biochemistry, M. S. University of Baroda, India.

B. Sc in Chemistry, Bombay University, Bombay India.

PUBLICATIONS

1. K. Alvares and A.S. Balasubramanian: Lysosomal and microsomal -glucuronidase of monkey brain: Differential elution characteristics from Con A-Sepharose and neutral sugar composition. Biochem. Biophys. Acta. (1982), 708, 124-133.

2. K. Alvares and A.S. Balasubramanian: A binding protein for lysosomal enzymes isolated from brain by phosphomannan-Sepharose chromatography. Biochem. Biophys. Res. Commun. (1983) 123, 398-406.

3.* A.S. Balasubramanian, T. Alam, S. Lakshmi, R. Mathur, R. Cherian and K. Alvares: The use of Concanavalin A-Sepharose in the purification and separation of multiple forms of brain hydrolases. J. Biosci. (1983) 5, 61-64.

4. R. Mathur, K. Alvares and A.S. Balasubramanian: Two forms of acid -mannosidase in monkey brain: Evidence for the co-existence of high mannose and complex oligosaccharides in one form. Biochem. Biophys. Res. Commun. (1984) 123, 1185-1194.

5. K. Alvares, K. Pannerselvam and A.S.Balasubramanian: The binding requirements of monkey brain lysosomal enzymes to their immobilized receptor protein. J. Biosci. (1986) 10, 215-225.

6. J. K. Reddy, M. S. Rao, N. D. Lalwani, M. K. Reddy, M. R. Nemali, and K. Alvares: Induction of peroxisome proliferation by xenobiotics. In "Peroxisomes in Biology and Medicine" Ed. by H. Fahimi and H. Sies. Springer-Verlag Berlin Heidelberg. (1987) 225 - 266.

7. N. D. Lalwani, K. Alvares, M. K. Reddy, M. N. Reddy, I. Parikh and J. K. Reddy: Peroxisome proliferators

binding protein: Identification and partial characterization of a nafenopin- clofibric acid- and ciprofibrate

binding proteins from rat liver. Proc. Natl. Acad. Sci. (USA), (1987) 84, 5342-5347.

8. K. Alvares and A. S. Balasubramanian: The lysosomal enzyme binding receptor from brain and its

characteristics. Indian J. Biochem. Biophys. (1988) 25, 150-151

9. R. S. Dwivedi, K. Alvares, M. R. Nemali, V. Subbarao, M. I. Usman, A.W. Rademaker, J. K Reddy and M.

S. Rao: Comparison of the peroxisome proliferator-induced pleiotropic response in the liver of nine strains of

mice. Toxicologic Pathology. (1989) 17(l), 16- 26.

10. K. Alvares, M.R. Nemali, P.G. Reddy, M. S. Rao and J. K. Reddy: The nucleotide sequence of a full-length

cDNA clone encoding rat liver urate oxidase. Biochem. Biophys. Res. Commun. (I989) 158, 991-995.

11. S. Thangada, K. Alvares, M. Mangino, M.I. Usman, M.S. Rao, and J.K. Reddy: An in vitro demonstration of

peroxisome proliferation and increase in -oxidation system mRNAs in cultured rat hepatocytes treated with

ciprofibrate. FEBS Letters. (1989) 250(2), 205-210

12. K. Alvares, A. Carrillo, P. M. Yaun, H. Kawano, R. I. Morimoto, and J. K. Reddy: Identification of cytosolic

peroxisome proliferator binding protein as a member of the heat shock protein HSP70 family. Proc. Natl.

Acad. Sci. (USA), (1990), 87, 5293-5297.

13. A. V. Yeldandi, X. Wang, K. Alvares, S. Kumar, M. S. Rao and J. K. Reddy: Human urate oxidase gene:

Cloning and partial sequence analysis reveals a stop codon within the fifth exon. Biochem. Biophys. Res.

Commun. (1990) 171, 641-646.

14. X. Wang, H. Kawano, K. Alvares, P. G. Reddy, H. Getto, M. S. Rao and J. K. Reddy: Rat urate oxidase:

Cloning and structural analysis of the gene and 5' flanking region. Gene. (1991) 97, 223 -229.

15. A. V. Yeldandi, V. Yeldandi, S. Kumar, C.V. N. Murthy, X. Wang, K. Alvares, M. S. Rao, and J. K. Reddy:

Molecular evolution of urate oxidase encoding gene in hominoid primates: nonsense mutations. Gene (1991)

109, 281-284.

16. K. Alvares, R. J. Widrow, G. Abu-Jawdeh, J. V. Schmidt, A. V. Yeldandi, M. S. Rao and J. K. Reddy: Rat

urate oxidase produced by recombinant baculovirus expression: Formation of peroxisome crystalloid core

like structures. Proc. Natl. Acad Sci. (USA), (1992) 89, 4908-4912.

17. B. Zhang, S. L. Marcus, F. G. Sajjadi, K. Alvares, J. K. Reddy, S. Subramani, R. A. Rachubinski and J.

Capone: Identification of a peroxisome proliferator-responsive element upstream of the gene encoding rat

peroxisomal enoyl-CoA hydratase / 3-hydroxyacyl CoA dehydrogenase. Proc. Natl. Acad. Sci. (1992) 89,

7541-7545.

18. M.S. Rao, H. Ide, K. Alvares, V. Subbarao, J.K. Reddy, O. Hechter and A.V. Yeldandi: Comparative effects

of dehydroepiandrosterone and related steroids on peroxisome proliferation in rat liver. Life Sciences. (1993)

52, 1709-1716.

19. Z. Z. Liu, J. Wada, K. Alvares, A. Kumar, E. I. Wallner,. and Y.S. Kanwar,.: Distribution and relevance of

IGF-I receptor in metanephric development. Kidney International. (1993) 44, 1242-1250.

20. J. Wada, Z. Z. Liu, K. Alvares, A. Kumar, E. I. Wallner, H. Makino, and Y. S. Kanwar,.: cDNA cloning of

-subunit of mouse IGF-I receptor and its role in metanepheric development. Pros. Natl. Acad Sci. (USA).

(I993), 90, 103**-*****.

21. Y. Zhu, K. Alvares, Q. Huang , M. S. Rao, and J. K. Reddy : Cloning of a new member of the peroxisome

proliferator activated receptor gene family from mouse liver. J. Biol. Chem. (1993) 268, 268**-*****.

22. Q. Huang , K. Alvares, C. A. Bradfield, R. Chu and J. K. Reddy : Association of peroxisome proliferators

activated receptor with HSP72. J . Biol. Chem. (I 994), 269, 8493-8497.

23. S. Hayashi, S. Jain , R. Chu, K. Alvares, B. Xu, F. Erfurth, N. Usuda, M. S. Rao, S. K. Reddy, Y. Noguchi ,

J.K. Reddy and A. V. Yeldandi : Amphibian Allantoinase; Molecular cloning, tissue distribution and

functional expression. J Biol Chem. (1994) 269, 122**-*****.

24. K. Alvares, C. Fan, S. S. Dadras, A. V. Yeldandi, R. A. Rachubinski, J. P. Capone, S. Subramani, P. M.

lannaccone, M. S. Rao and J. K. Reddy : An Upstream Region of the Enoyl-CoA hydratase / 3-hydroxyacyl

CoA dehydrogenase gene directs luciferase expression in liver in response to peroxisome proliferators in

transgenic mice. Cancer Research, (1994) 54, 2303-2306.

25. R. Chu, N. Usuda, M. K. Reddy, C. Liu, Y. Hashimoto, K. Alvares, M. S. Rao and J. K. Reddy Functional

Expression of rat peroxisomal Acyl-CoA oxidase in Spodoptera frugiperda Cells. Biochem. Biophys. Res.

Commun. (1994), 200, 178-186.

26. N. Usuda, S. Hayashi, S. Fugiwara, T. Noguchi, T. Nagata, M. S. Rao, K. Alvares, J. K. Reddy and A. V.

Yeldandi: Uric acid degrading Enzymes Urate Oxidase and Allantoinase are associated with different

subcellular organelles in frog liver and kidney. J. Cell Science. (1994) 107,1073-1081.

27. J. K. Reddy and K. Alvares: Perspective on Hepatic Peroxisomes and Pancreatic Hepatocytes:

Immunocytochemical Approaches. Med. Electron Microscopy. (1994) 27 (3-4), 179-188

28. Q.Huang, A. V. Yeldandi, K. Alvares, H. Ide, J. K. Reddy and M. S. Rao: Localization of peroxisome

proliferator activated receptor in mouse and rat tissues and demonstration of its nuclear translocation in

transfected CV-1 cells. Int. J. Oncology (1995) 6, 307-312.

29. S. Chu, Q. Huang, K. Alvares, A. V. Yeldandi, M. S. Rao, and J. K. Reddy.: Transformation of

mammalian cells by overexpressing H2O2-generating peroxisomal fatty acyl-CoA oxidase. Proc. Natl.

Acad. Sci. (USA). (1995) 92, 7080-7084.

30. K. Alvares, V. Subarao, M. S. Rao and J. K. Reddy: Ciprofibrate represses  2u-globulin expression in liver

and inhibits D-limonene nephrotoxicity. Carcinogenesis, (1996) 17(2), 311-316

31. A Veis, J. Malone and K. Alvares. Type 1 procollagen heterotrimer assembly is linked to subtle

differences in the structures of the pro1(1) and pro-2(1) carboxyl-propeptides. Proc. Indian

Acad Sci, (1999), 111, 115-120

32. K. Alvares, F. Siddiqui, J. Malone and A. Veis: Assembly of the type I procollagen molecule:

Selectivity of the interactions between the 1(1) and 2(1)-carboxyl propeptides. Biochemistry

(1999), 38(17), 5401-5411

33. A.Veis, K. Tompkins, K. Alvares, A. G. Brownell, K. Wei, L.Wang, X.Wang, S.M. Jengh, and Kevin E.

Healy: Specific Amelogenin Gene Splice Products have Signaling Effects on Cells in Culture and in

Implants In Vivo. J. Biol. Chem. (2000) 275, 412**-*****.

34. K. Tompkins, K. Alvares, A. George and A. Veis : Two related low molecular mass polypeptide

isoforms of amelogenin have distinct activities in mouse tooth germ differentiation, in Vitro. J. Bone

and. Mineral Research (2005) 20, 341-349

35. J. Malone., K. Alvares and A. Veis: Structure and Assembly of the heterotrimeric and Homotrimeric C-

propeptides of Type I collagen. Significance of the 2(1) chain. Biochemistry (2005) 44(46); 152**-*****

36. K. Alvares, Y. S. Kanwar, and A. Veis. Expression and Potential Role of Dentin Phosphophoryn (DPP) in

Mouse Embryonic Tissues Involved in Epithelial- Mesenchymal Interactions and Branching Morphogenesis.

Dev. Dynam. (2006), 235, 2980–2990,

37. J. Khoshnoodi, J.P. Cartailler, K. Alvares, A. Veis, and B.G. Hudson: Molecular Recognition in the

Assembly of Collagens: Terminal Noncollagenous Domains are Key Recognition Modules in the Formation

of Triple-Helical Protomers: J. Biol. Chem. (2006), 286, 38117 – 38121.

38. Veis, A., Dixit, S.N., Barss, J.T., Robach, J.S., Alvares, K., Malone, J.P., Lux, E., and Stock, S.R: Mineral

Matrix Relationships in the Developing Tooth of the Sea Urchin, Lytechinus variegatus. In:

Biomineralization: From Paleontology to Materials Science, J.L. Arias, M.S. Fernández eds., Editorial

Universitaria, Santiago, Chile (2006)

39. K. Alvares, S.N. Dixit, E. Lux, J. Barss and A. Veis: The Proteome of the Developing Tooth of the Sea

Urchin, Lytechinus Variegatus: Mortalin is a Constituent of the Developing Cell Syncytium: Journal of

Experimental Zoology. Part B. Molecular and Developmental Evolution (2007) 308B, 357-370.

40. A. Veis, K. Alvares, S.N.Dixit, J.S. Robach and S.R. Stock.Characterization of two distinctly different

mineral-related proteins from the teeth of the Camarodont sea urchin Lytechinus variegatus: Specificity of

function with relation to mineralization. Front.Mater. Sci. (2009) 3(2) 163-168

41. Keith Alvares, Saryu N. Dixit, Elizabeth Lux, and Arthur Veis.: Echinoderm Phosphorylated Matrix Proteins

UTMP16 and UTMP19 Have Different Functions in Sea Urchin Tooth Mineralization. J. Biol Chem. (2009)

284, 26149- 16160

.

Contact this candidate