Quality Assurance Training
Resume:
Curriculum Vitae
Objective Working in an exciting and technology intensive atmosphere, which would give me opportunities to learn new things.
Areas of Interest Polychromatic Flow Cytometry, Medical Microbiology, Molecular Biology,
Education
• 2006-2011 Ph.D., thesis submitted (awaiting viva)
All India Institute of Medical Sciences, New Delhi. (Submitted in C.C.S.University, Meerut, U.P., India).
• 2002-2004 M.Sc. (Microbiology)
C.C.S.University, Meerut, U.P., India.
• 1994-1997 B.Sc. (Zoology, Botany, Chemistry)
C.C.S.University, Meerut, U.P., India.
Honors and Awards
Feb, 2008- till date Research Officer awarded by National AIDS Control Organization (NACO), Govt. of India.
May, 2005-Feb, 2008 Junior research fellow and subsequently as senior research fellow awarded by Indian Council of Medical Research (ICMR), Govt. of India.
March 27-1Apr.,2008 Global Health Travel award (Bill and Melinda gates foundation) to attend and participate in Keystone symposium: “HIV Pathogenesis” Banff Alberta, Canada.
List of Publications
• Chauhan N.K., M.Vajpayee, K.Mojumdar, R.Singh, A.Singh, S.Kurapati. Manuscript entitled “Study of CD4+CD8+ Double Positive T Lymphocyte Activation and Exhaustion in HIV-1 Infected Indian Patients”. (Accepted, J of Medical Virology)
• Chauhan N.K., M.Vajpayee, K.Mojumdar, S.Kurapati, N.Wig, J.C.Samantaray. Manuscript entitled “Usefulness of haemoglobin and albumin as alternate prognostic markers for highly active antiretroviral therapy for HIV-1 Infection”. (Under revision, Indian J Med Res)
• Alpana Singh, Madhu Vajpayee, Sharique A. Ali, Kamalika Mojumdar, Neeraj Kumar Chauhan, Ravinder Singh. Manuscript entitled “HIV-1 disease progression associated with loss of Th17 cells in subtype C infection”. (Under revision, Cytokine)
• Mojumdar K, M.Vajpayee, N.K. Chauhan, A.Singh, R.Singh, S.Kurapati. Manuscript entitled “Altered T- cell differentiation associated with loss of CD27 and CD28, a hallmark of HIV infection”. Cytometry B Clin Cytom. 2011 Jun 21. doi: 10.1002/cyto.b.20610. [Epub ahead of print]
• Mojumdar K, M.Vajpayee N.K. Chauhan , A.Singh, R.Singh, S.Kurapati. Manuscript entitled “Loss of CD127expression and increased immunosenescence of T-cell subsets in HIV infected individuals”. (Accepted, Indian J Med Res)
• Mendiratta S, M.Vajpayee, K.Mojumdar, N.K.Chauhan, V.Sreenivas. Polyfunctional analysis of Gag and Nef specific CD8+ T-cell responses in HIV-1 infected Indian individuals. Vaccine. 2010 Dec 17.
• Mojumdar K, M.Vajpayee, N.K.Chauhan, S.Mendiratta, N.Wig. Defects in Blood Dendritic cells subsets in HIV-1 subtype C infected Indians. Indian J Med Res. 2010 Sep; 132:318-27
• Mojumdar K, M.Vajpayee, N.K.Chauhan, S.Mendiratta. Late presenters to HIV care and treatment, identification of associated risk factors in HIV-1 infected Indian population. BMC Public Health. 2010 Jul 13;10:416.
• Chauhan N.K., S.Mendiratta, K.Mojumdar, M.Vajpayee. Clinical, Hematological, Immunological Characteristics and Therapeutic Intervention in Children Born to HIV Infected Mothers in India. Journal of Pediatric Infectious Diseases. 2010 Jan Vol 5 No. 1
• Mendiratta S, M.Vajpayee, U.Malhotra, S.Kaushik, L.Dar, K.Mojumdar, N.K.Chauhan. Characterization of Gag and Nef specific Elispot Based CTL responses in HIV infected Indian individuals. Med Microbiol Immunol. 2009 Feb;198(1):47-56. Epub 2008 Nov 5.
• Vajpayee M, S.Kaushik, V. Sreenivas, K.Mojumdar, S.Mendiratta, N.K.Chauhan. Role of immune activation in CD4(+) T-cell depletion in HIV-1 infected Indian patients. Eur J Clin Microbiol Infect Dis. 2009 Jan;28(1):69-73. Epub 2008 Jul 9.
• Mathur P, J.C.Samantaray, N.K.Chauhan. Evaluation of a rapid immunochromatographic test for diagnosis of kala-azar & post kala-azar dermal leishmaniasis at a tertiary care centre of north India. Indian J Med Res 122, December 2005, pp 485-490.
Abstracts
• Poster presentation for abstract entitled Manuscript entitled “Immunodynamics of Th17 cells in HIV-1 subtype C infection” Alpana Singh, Madhu Vajpayee, Sharique A. Ali, Neeraj Kumar Chauhan, Ravinder Singh. (Submitted in International Science Symposium on HIV and Infectious Diseases). 20th-22th January 2012, Chennai, India.
• Poster presentation for abstract entitled “Efficacy of Singly Dose Nevirapine Therapy in HIV PMTCT” Madhu Vajpayee, Kamalika Mojumdar, Neeraj K. Chauhan, Alpana Singh, Ravinder Singh. (Accepted in 7th World Congress of the World Society for Pediatric Infectious Disease (WSPID). 16th -19th November 2011, Melbourne, Australia.
• Poster presentation for abstract entitled Manuscript entitled “Study of CD4+CD8+ Double Positive T Lymphocyte Activation and Exhaustion in HIV-1 Infected Indian Patients” M.Vajpayee, N.Chauhan, K.Mojumdar, R.Singh, A.Singh. International AIDS Society (IAS). 17-20th July 2011, Rome, Italy.
• Oral presentation entitled “Differential expression of CD38 on CD8 T-cells in HAART naïve and successful HAART treated HIV-1 infected india individuals” Neeraj Kumar Chauhan, Madhu Vajpayee, Kamalika Mojumdar, Sanjay Mendiratta, Magan Kumar Agarwal. International Conference on Opportunistic Pathogens in AIDS (ICOPA). 27th –30th September 2010, New Delhi, India.
• Poster presentation for abstract entitled “Usefulness of haemoglobin and albumin as alternate prognostic markers for highly active antiretroviral therapy for HIV-1 Infection” Neeraj Kumar Chauhan, Madhu Vajpayee, Kamalika Mojumdar. XVIII International AIDS Conference 18 – 23rd July 2010, Vienna, Austria. Abstract number A-240-****-*****.
• Poster presentation for abstract entitled “Effect of Dendritic Cell Loss on T cell Immune Responses against HIV gag in HIV Infected Indian Individuals” Kamalika Mojumdar, Madhu Vajpayee, Neeraj Kumar Chauhan. XVIII International AIDS Conference 18 – 23rd July 2010, Vienna, Austria. Abstract number A-240-****-*****. (Awarded scholarship to attend the conference)
• Poster presentation for abstract entitled “Analysis of blood dendritic cell subsets in HIV-1 infected and uninfected Indian individuals” Madhu Vajpayee, Kamalika Mojumdar, Neeraj Kumar Chauhan. XVIII International AIDS Conference 18 – 23rd July 2010, Vienna, Austria. Abstract number A-240-****-*****.
• Poster presentation for abstract entitled “Defects in Blood Dendritic cells in HIV-1 Subtype C infected Indian individuals” Kamalika Mojumdar, Madhu Vajpayee, Sanjay Mendiratta, Neeraj K Chauhan. FOCIS 2009 June11-14, San Francisco, USA. Abstract no. S6.
• Poster presentation for abstract entitled “Gag and Nef specific functional analysis of T-Cell responses in HIV-1 infected Indian individuals” Sanjay Mendiratta, Madhu Vajpayee, Neeraj Kumar Chauhan, Kamalika Mojumdar. Keystone Symposia on HIV Immunobiology (X4) March 22–March 27, 2009. Keystone, Colorado, USA
• Oral presentation entitled “Single Cell analysis of IFN-γ ,IL-2, IL-4, IL-10 production by CD4 T-Cells from HIV infected and uninfected individuals” Chauhan NK, Vajpayee M, Dar L, Mojumdar K, Mendiratta S. ICOPA India: International Conference on Opportunistic Pathogens in AIDS. 27th –29th Jan 2008, New Delhi, India.
• Poster presentation for abstract entitled “Analysis of Intracellular Cytokine Profile of CD4+ T-cell and study of Th1 to Th2 shift in HIV-1 infected and uninfected Indian Individuals” Neeraj Kumar Chauhan, Madhu Vajpayee, Sanjay Mendiratta, Kamalika Mojumdar, Lalit Dar. Keystome Symposia on HIV pathogenesis(X8) March 27 - April 1, 2008. Fairmont Banff Springs, Banff.
• Poster presentation for abstract entitled “Hemoglobin and serum albumin as surrogate marker for HIV monitoring in resource-limited settings” Vajpayee M, Mendiratta S, Chauhan NK, Mojumdar K. : AIDS 2008 - XVII International AIDS Conference: Abstract no. CDB0005"
Workshop/Training organized
• Training Workshop organized on “External Quality Assurance Scheme g” 18th April, 2011. Department of Microbiology AIIMS New Delhi, India.
• Training Workshop organized on “External Quality Assurance Scheme for HIV Testing” 26th March, 2010. Department of Microbiology AIIMS New Delhi, India.
• Training Workshop organized on “ICTC Sensitization Training Programme” 13-14th February, 2009. Department of Microbiology AIIMS New Delhi, India.
Workshop/Training
• Training on “Calibration of the instruments” 09 to 10 March, 2010. Department of Microbiology AIIMS New Delhi, India.
• Training on “Estimation of CD4 by FACS Count” 15-16 July, 2009. Department of Microbiology AIIMS New Delhi, India.
• Training on “ELISA for HIV testing” 20 to 25 July, 2009. Department of Microbiology AIIMS New Delhi, India.
• Training course to perform the Cavidi ExaVir Load Version 3, HIV RT viral load assay. Training officer, Kajsa Lowenhielm, CAVIDI AB, SWEDEN.
• Workshop on NACO-EQAS-North Zone Workshop For State Reference Laboratories held at NIB NOIDA From 30 march to 2 April 2009.
• Training workshop for Dried Blood Spot testing Under HIV Sentinel Surveillance (HSS) held at NARI on 19 to 20 March 2009.
• Training workshop on “ICTC Sensitization Training Programme” held at Department of Microbiology, AIIMS, from 13-14 February 2009.
• Workshop on “HIV External Quality Assessment Scheme” held at Safdarjang Hospital 16 December, 2008.
• Workshop on “How to write a paper” March 9 to 10, 2007. AIIMS New Delhi India.
• Basic Flow Cytometry Course from 18th to 20th Jan 2006, on BD FACSCalibur at BD Biosciences Training center, Gurgaon, India.
• Workshop on “External Quality Assurance Scheme For HIV Testing”. December 27 to 29, 2005. Department of Microbiology AIIMS New Delhi, India.
Conference
• International Conference on “Opportunistic Pathogens in AIDS (ICOPA) “. 27th –30th September 2010, New Delhi, India.
• International Conference on “HIV Pathogenesis “March 27 – April 1, 2008 Banff Alberta Canada.
• International Conference on “Opportunistic Pathogen in AIDS “ January 27 to 29,2008 AIIMS New Delhi India
• Conference on “33rd Annual Conference of the Indian Immunology Society” January 28 to 31, 2007 AIIMS New Delhi India.
• Conference on “HIV / AIDS and TB: 2006” January 13-15, 2006.AIIMS New Delhi India.
Research Experience
• August 2010 - onwards: Research Officer in HIV-Immunology laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
• May 2006 – to August 2010: Worked as a Ph.D. student on the thesis entitled “Evaluation of Cytokine Profile and TH1/TH2 shift in HIV-1 (Subtype C) infected Indian patients” under supervision of Dr. M. K. Agarwal (C.C.S.University Meerut) and Co-supervision Dr. Madhu Vajpayee (All India Institute of Medical Sciences, New Delhi).
• Major techniques practiced during Ph.D.:
• Immunophenotyping of various T-cell surface markers and measurement of intracellular cytokines by Flow Cytometry using whole blood and PBMCs.
• Data analysis and interpretation by Flowjo and CELLQuest software on FACS Calibur and BD LSR II instruments.
• IFN- ELISpot assay using 15 mer and 20 mer overlapping HIV-1 peptides.
• Data reporting and analysis by ELISpot reader (Carl Zeiss, CTL Immunospot)
• Determination of CD4+ and CD8+ T-cell counts and their subsets
• Determination of HIV-DNA using Amplicor HIV-1 DNA test kit (Roche Diagnostics)
• Quantitation of plasma HIV-RNA using COBAS TaqMan48, Real Time PCR (Roche Diagnostics)
• HIV diagnosis by Enzyme-linked Immunosorbent assay (ELISA), Rapid tests and Western Blot.
• May 2006 – Nov 2009: Along with the thesis work, I worked with Gynecology Department on “Clinical, Hematological, Immunological Characteristics and Therapeutic Intervention in Children Born to HIV Infected Mothers in India.
• January 2004 – June 2004: M.Sc. Dissertation entitled “Application of newer techniques for diagnosis of leishmaniasis” under Dr J C Samantaray in Department of Microbiology, AIIMS, New Delhi.
Personal Details:
Father name Dayachand Chauhan
Nationality Indian
Date of Birth July 15, 1977
Marital status Married
Sex Male
Address for Correspondence (Official)
Room No. 2062, HIV Immunology Division, Department of Microbiology
All India Institute of Medical Sciences (A.I.I.M.S)
New Delhi, India - 110029
Tel: 91-11-265***** Fax: 91-11-265*****
Address for Correspondence (Residence)
S/0 Dayachand Chauhan, Vill- Dhikoli
Post- Mawana, Distt- Meerut (U.P), India
Tel: 012**-****** Mobile: 91-986*******
E-mail: **************@*****.**.**
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