Project Quality Control
Resume:
Malini Shankar
Lake Villa, IL 60046
***************@*****.***
SUMMARY
Multilingual professional with eighteen years in various scientific and medical research areas looking for a position utilizing education, training and experience in research, quality control and purchasing.
WORK EXPERIENCE
August 2008 – Present University of Iowa, Iowa City, IA
Research Associate
I have been working at the University of Iowa’s Department of Pediatrics since August of 2008 to the present. My research work is with the Department of Ophthalmology & Visual Sciences. I have been involved in Vision Research where the major focus has been to work on Retinal Degenerative diseases. My major role has been in management, analysis & interpretation of all the data generated in the lab using various software tools. My work so far in this lab has resulted in a publication in the Journal of Neurophysiology in March 2011. Currently have another manuscript ready for submission by July 22nd 2012.
The list of analysis tools & software that I work with are as follows:
PowerNAP 0.7- Raw data analysis
Offlinesorter V3- Raw data analysis
MATLAB- Computation of data post analysis
Origin 8- Statistical calculations & figure generation
Duties & Responsibilities: I Mentor & train Post Docs, graduate students & undergraduate students in use of the data analysis software. I am also the Website Manager & Virtual Server Manager. Additionally, I handle all the Purchasing for the lab. I am well versed with the use of the P-Card (Procurement Card) & also the Pro-Trav system for all travel purchases for the lab. I regularly interact with Vendors and manage the relationship for purchase of various software tools required for our projects. I am involved in preparing all the documents needed for the various Environmental Health & Safety Audits. I prepare PowerPoint Presentations for all the data at the Department Meetings.
November 2006 – May 2008 Reddy US Therapeutics, Atlanta, GA
Scientist
Reddy US Therapeutics is dedicated to discovering novel therapeutics that addresses unmet medical needs in diabetes, cardiovascular disease, anti-infective, inflammation & cancer.
My major area of focus was Reverse Cholesterol Transport.
The following is a list of techniques I worked on a regular basis for this project:
High Throughput screening of potential drug development candidates using following techniques:
In Vitro Studies.
Real Time PCR; Transfections; Tissue culture; Luciferase Assays; Protein Assays &Cell Free based assays.
InVivo Studies
Tissue collection & preps’, Histo staining of Tissue sections & Immunostaining of tissue sections.
Mechanism of Action Studies
Plasmid cloning, Coimmunoprecipitation, Western blots & siRNA knockdown.
I coordinated the projects, project management, data analysis & data interpretation & generation of reports for this project to make sure that we kept up with the timelines.
Duties & Responsibilities: I carried out general Lab Management duties, in addition to being a Buyer & Purchasing Agent for the company. In this role I have gained experience in identifying vendors, managing relationships, identifying alternate sources and interacting with them constantly which have translated into cost saving for the company. This has given me experience in generating Purchase Orders & handling Logistics issues. Additionally, I also played a role in Budget Management for the company.
November 1999 – September 2006 Oklahoma Medical Research Foundation (OMRF)
Oklahoma City, OK
OMRF is a nonprofit biomedical research institute dedicated to understanding & curing human disease
Senior Research Assistant- Immunobiology & Cancer
I worked with Dr. Carol Webb from March 2004 to September 2006. I had my own independent project. My major project involved studying a Transgenic Mouse Model which was generated by our lab. I used all the below mentioned techniques to characterize this mouse model which eventually lead my first author publication in the Journal of Immunology.
Another project that I was involved was the Role of Acute Phase Proteins in macrophage survival in response to Lethal Toxins (specifically Anthrax) published in Journal of Cellular Immunology October 2011.
Molecular Biology
PCR (designing PCR primers, cloning PCR products, RT/PCR), Generating radioactive probes (nick translation, kinasing), Plasmid cloning (digests, ligations, electroporation, colony screening, DNA preps), Southern blots (digests, gel, blot, probing) & Tissue Culture
Mice Work
I have experience in managing large mouse colony (husbandry, genotyping, weaning, timed mating, and supplying experimental animals at defined postnatal time points).
Collection of mouse organs, Bone marrow cells, Spleen cells, Peritoneal cells, Thymus cells & Blood .
Other Techniques in which I gained vast experience are:
Western Blots, Protein extracts, nuclear extracts, Electron Mobility Shift Assay, ELISA, Cell Staining, Perfusions, Immunization, and Flow Cytometry.
Have good experience with data analysis & data interpretation.
Duties & Responsibilities: I was involved in mentoring programs for graduate students, undergraduate students & high school students. I also performed management duties for the group of Junior Research Assistants working directly under my supervision. I was in charge of Purchasing for the lab which involved identifying & interacting with Vendors on a regular basis, I also played a key role in Budget Management for the lab.
Research Assistant - Developmental Biology
I worked with Dr. Ute Hochgeschwender from December 2002 until February 2004. My major project in this lab was to create a Knock Out Mouse Model. I gained significant experience in the following techniques while working on this project:
Molecular Biology
PCR (designing PCR primers, cloning PCR products, RT/PCR), Generating radioactive probes (nick translation, kinasing), Plasmid cloning (digests, ligations, electroporation, colony screening, DNA preps), Screening genomic phage library (lifts, plaque purifications, phage DNA prep), Northern blots (RNA preparation, gel, blot, probing), Southern blots (digests, gel, blot, probing), Homologous recombination in E. coli (E/T cloning), Tissue Culture, Embryonic fibroblasts (maintenance, passage, freezing) & Embryonic stem cells (maintenance, passage, freezing)
Mice Work
Managing large mouse colony (husbandry, genotyping, weaning, timed mating, supplying experimental animals at defined postnatal time points)
Collection of mouse organs by surgery & perfusion techniques.
Associate Research Assistant- Molecular & Cell biology
I worked with Dr. Robert Barstead in the Molecular & Cell Biology Department for 3 years. I was part of the HighThroughput C.Elegans Gene Knockout Project. My role in this project was to screen the worm DNA by PCR.
Results helped in locating the mutant worms from the mixed population of mutant & wild type worms.
My job description involved data analysis & data interpretation.
Duties & Responsibilities: In addition to my lab duties, I was involved in various administrative aspects, which included data management; project coordination; inventory control, training new employees. I gained valuable management experience since I had a group of Junior Research Assistants under my direct supervision.
February 1999 - December 2000 Rose State College, Midwest City, OK
Adjunct Faculty Biology
Rose State College is a two-year, public comprehensive community college, which provides local and wide-area commuter students with an array of learning opportunities. The college has approximately 12,000 credit students and more than 30,000 continuing students annually.
My appointment involved teaching introductory and general college level biology classes and labs, in addition to tutoring students in Biology; Zoology; Chemistry and Biochemistry.
June 1995 - February 1999. Big D Industries, Inc., Oklahoma City, OK
Chemist & Quality Control Manager
Big D Industries is a leader in the manufacture of odor control products.
My function was to conduct research, to develop new products, using my experience and academic training in the area of bioenzymatic processes and the ability to identify and specify strains of bacteria (nonpathogenic) that can be successfully incorporated in a bacterial cleaning compound. I also developed and implemented Q.C. procedures to test the concentration of bacterial spores in the product. I carried out routine quality control of incoming and outgoing products by gas chromatography & Karl Fisher moisture titration.
May 1994- June 1995. Raptakos & Brett, Mumbai, India
Biochemist
Raptakos & Brett is a diagnostic center in Mumbai, India, specializing in bio-chemical analysis. My duties were to perform human biochemical profile analysis and generate reports to document those tests and results.
COMPUTER & WRITING SKILLS
Proficient with use of Microsoft Office, Microsoft Access, Adobe Dreamweaver, Endnotes, Adobe Photoshop & the Web. Have experience in using Mac also for data analysis. I have extensive experience with writing portions of my first author paper.
PUBLICATIONS
1. Developmental time course distinguishes changes in spontaneous & light evoked retinal ganglion cell in rd1 & rd10 mice. Published by Journal of Neurophysiology, March 2011; 105: 2560-2571 - Stasheff Steven; Shankar Malini and Andrews MP.
2. Serum Amyloid A Protects Murine Macrophages from Lethal Toxin-Mediated Death. Published by Journal of Cellular Immunology on October 29, 2011; CIMM-11-151R2- Kira Rose; Paul Long; Malini Shankar; Jimmy D Ballard and Carol F. Webb.
3. Anti-nuclear Antibody Production and Autoimmunity in Transgenic Mice that Over-express the Transcription Factor Bright. Published by The Journal of Immunology, March 2007 178:2996-3006 - Malini Shankar; Jamee C. Nixon; Shannon Maier; Jennifer Workman; A. Darise Farris and Carol F. Webb
4. Development, Maintenance, and Function of the Adrenal Gland in Early Postnatal Pro-opiomelanocortin Null Mutant Mice. Published by Journal of Endocrinology on February 24, 2005 as doi:10.1210/en.2004-1290 - Jason Karpac; Dirk Ostwald; Stephanie Bui; Peggy Hunnewell; Malini Shankar, and. Ute Hochgeschwender.
POSTER
Anti-nuclear Antibody Production and Autoimmunity in Transgenic Mice that Over-express the Transcription Factor Bright - Malini Shankar, Jamee C. Nixon, Shannon Maier, Jennifer Workman, A. Darise Farris and Carol F. Webb. Oklahoma Medical Research Foundation, Oklahoma City, OK 73104
EDUCATION
2010 Biomedical Professional Development Series University of Iowa, Iowa
1994 M.Sc. Biochemistry University of Mumbai, India
1992 B.Sc. Botany and Biochemistry University of Mumbai, India
STRENGTHS
I am capable to work on independent projects. I have excellent organizational and communicative skills; I am self-motivated and willing to make strong commitment to goals, conscientious and reliable with a good problem solving aptitude.
REFERENCES
Provided on request.
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