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analytical chemist

United States
May 30, 2010

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Tao Zhang (permanent resident)

**** ** ***** **, #***,

houston, tx, 77054

Tel: 215-***-****(cell), 713-***-****(home)



A challenging research and development chemist position that utilizes my experience in analytical chemistry and biochemistry studies, and cGMP experience in companies in small molecular and proteins, siRNAs, polymers and liposome

Summary of Qualification

· cGMP experience in pharmaceutical industry research and development, QC

· Analytical testing of drug products and raw material, method transfer/validations, instrument validations, writing protocols, initiate and perform investigations, document work, tests accurately in according with cGMP requirements, data interpret, ADME, DM/PK knowledge

· 10-Year experiences of analytical chemistry research on both small molecules and proteins, siRNAs, writing protocols, method development and validation of HPLC experience, and technical transfer

· 3-Year physical Solid characterization (XPRD, TGA/GSC, NMR,SEM, and VTI, Karl-fisher, KF)

· 7-Year experience in method development in protein expression and purification, characterization, protein assays, protein interaction with ligands, ELISA, gel electrophoresis, and Western blot

· Liposome and polymers, siRNA and nature compounds purification and characterization

Technique and Skills

· Method development and validation on UPLC, UPLC-MS, HPLC, LC-MS, GPC, Ion exchange, LC/MS/MS and technical transfer, familiar with Empower system methods transfer, and chemstation, familiar Waters, Agilent, Shimadzu and Beckman HPLC machines

· GC, GC-MS, Liquid NMR, microscopy, TLC, FT-IR, UV-Vis, fluorescence spectroscopy, Karl-Fisher(KF)

· TGA/DSC, capillary DSC (VPDSC), SEM, XRD (XRPD), particle size, zeta potential, light scanner, ITC titration,

· Raw materials (APIs, small molecules and polymers, liposome) test of stability, solubility, dissolution, related substance and force degradation, content uniformity

· Proteins assays and siRNA assay, light scattering, fluorescence, circular dichroism(CD), formulations, Evaluation of enzyme activity and inhibitor actions (ELISA), Solid-phase synthesis of peptides

· SDS-Page, Nu-Page gel electrophoresis, Western blot

· Over-expression and purification (FPLC, HPLC, LC/MS) of recombinant proteins, solid phase extraction (SPE)

· Proficiency in Microsoft Office applications, power point, Data processing, report and evaluation, SGI work station with UNIX or LINUX operation system, BASIC language

Professional Experience

2009/05-2010/04 Analytical chemist(contract) in Wyeth, Pearl River, NY

· Method development, validation and transfer on API, in-process, finished products and impurities test by KF, LCMS, UPLC and UPLC-MS with Empower system and Chemstation with cGMP regulated, support formulation and clinical trail materials.

2008/03-2008/10 Analytical chemist (Contract) in Merck, West Point, PA

· Supported the formulation by doing siRNA analytical chemistry research and development, assays development, release, particle size, stability and encapsulation test, lipids analysis by SAX-HPLC, RPHPLC, GPC, and capillary DSC within GLP environments

2007/07-2008/03 Analytical Chemist (Contract) with URL-Mutual Pharm. Inc.

· Evaluated the raw material and finished products of stability (under FDA ICH regulation), related substances, force degradation by HPLC, LCMS (ESI), GC, GC-MS and DSC, KF, particle sizing, dosage dissolution testing, microscopy and FTIR to help research and development under cGMP regulation, and support the formulation of generic drugs

2007/01-2007/07 Analyst (Contract) with GSK (PP&D), Collegeville, PA

· Worked on the HPLC, LC-MS method, development and validation of preclinical compounds

· Evaluated the stability, solubility, force degradation, by HPLC, LC-MS (ESI, APCI), TGA, DSC and VTI to help the development and formulation under GLP regulation

2002/07-2006/12 Research Associate, University of Pennsylvania

· Completed an NIH sponsored study by executing key technical steps, the general inhaled anesthetics interaction with proteins

· Synthesized and characterized four 4-helix bundle proteins

· Method Development of proteins purification, purified and characterization

· Quantitative analysis of inhaled anesthetics, and proteins

· Elucidated the mechanism the action of anesthetics in these proteins using NMR and crystallography methods (2.7A resolution)

· Carried out extensive biophysics study on these proteins and inhaled anesthetics

· HPLC and Gel Permeation Chromatograph (GPC) method development, upgrade

· Collaborated, at the same time, with other departments by attending group meetings, Biophysics Society Annual Conferences, effectively communicating progresses to management

2000-2002 Visiting Scientist, Protein chemistry in LBC, NHLBI, NIH, Bethesda, MD

· Succeeded in the expression and purification of NKx2.5 homeo-domain protein and its mutant N51K protein, and carried out further characterization with protein quantitative analysis, CD, 1D, 2D, 3D NMR methods

· HPLC Method development on NKx2.5 protein purification and protein assay

· First in literature link to the homeo-domain NKx2.5/DNA complex binding affinity different with mutant N51K/DNA complex, the cardiologic development default caused by gene mutations

1997-2000 Research Assistant in liquid NMR, structural analytical Chemistry, the Chinese Academy of Sciences

· Developed geometry average method to measure water-hydroxyl exchange rates by 2D NMR method

· Used magnetic fields method to determine the proton-proton correlation times and distance of some peptides

· Structural analysis and bio-analysis of some nature extracts compounds

1994-1997 Research Assistant in analytical chemistry, Wuhan University, China

· Quantitative determine some trace ions by synthesized fluorescence reagents

· Provided analytical chemistry experiment training to the undergraduate students of Wuhan University

1991-1994 Chemical Engineer, Xintai Chemical Company, Xintai, China

· Quality control chemist on agriculture residues and fertilize, pesticides analysis. QC lab management


1997, M. S., Analytical Chemistry, Wuhan University, Wuhan, China

1991, B.S., Analytical Chemistry, Wuhan University, Wuhan, China

Publication:( selected from over 20 publication)

1. Tao Zhang and Jonas S. Johansson. “A Calorimetry Study on the Binding of six General Anesthetics to the Hydrophobic Core of a model Protein”. Biophys. Chem. 2005, 113,169~174

2. Tao Zhang and Jonas S. Johansson. “An Isothermal Titration Calorimetry Study on the Binding of four Volatile General Anesthetics to the Hydrophobic Core of a Four-a-helix Bundle Protein”. Biophys J. 2003, 85, 3279.

RREFERENCES (Available upon request)

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