Ruduan Wang

Mystic, CT ***** | 860-***-**** | w3cklv@r.postjobfree.com

SUMMARY

Accomplished biologist with extensive research experience in pharmaceutical industry and academia. Significant skills in cell culture, cell-based and biochemical assay development, receptor binding assays, immunoassays, RT-qPCR, gene cloning and transfection, protein expression and analysis, target evaluation and validation, biomarker identification and quantification. Familiar with diverse assay formats and detection modes. Self-motivated, energetic, and detail-orientated with strong problem-solving capability; good team player with significant experience working in matrix team environments.

TECHNICAL SKILLS

• Cell culture: primary mammalian cells (PBMC, lymphocytes, macrophages, neuron, glial cells, enterocytes) and cell lines

• Cell based assays (proliferation, apoptosis assay, cytotoxic assays, cytokine bioassays, cAMP HTRF assay)

• Radioligand and fluorescent ligand receptor binding assays

• Protein isolation and quantification

• SDS-PAGE and Western blotting

• Flow cytometry

• ELISA & Luminex assays

• Immunohistochemistry & Cellomics analysis

• DNA and RNA extraction and purification

• PCR & RT-qPCR

• Gene cloning and transfection

• QuantiGene assay & QuantiGene ViewRNA assay

• Animal handling, dosing, blood harvest and tissue collection

• Data analysis (Excel, Graphpad Prism, Sights & Spotfire)

PROFESSIONAL EXPERIENCE

BRISTOL-MYERS SQUIBB, WALLINGFORD, CT

Research Scientist (temp) Jan. 2012 - present

• Neuroscience: Executed primary neuronal culture, gene transfection, shRNA lentivirus transduction, RT-qPCR, QuantiGene 2.0 assay, Western blotting, immunocytochemistry staining and Cellomics analysis

• Virology: Developed and validated a 384-well virus capsid assembly assay; Generated Gag-YFP and Gag-CFP gene constructs; established RepRluc/Gag clones; performed transfection and virus titration

PFIZER INC., GROTON, CT 2001-2011

Senior Scientist, Diabetes, Department of Cardiovascular, Metabolic and Endocrine Diseases 2008-2011

• Developed a 384-well scintillation proximity binding assay for high-throughput screening against GPCR119; developed and executed an ex vivo receptor occupancy assay for analyzing GHSR1a antagonists; executed compound screening and characterization using ELISA, receptor binding assays, and cAMP HTRF assay

• Generated gene constructs and stable transfected cell lines; isolated and characterized proteins from tranfected cell lines and virus tranduced cells; profiled the gene expression of GPCR119, GLP1R, GIP, insulin, and glucagon in mouse, rat and human tissues using RT-qPCR; mapped the cellular distribution of multiple genes human islets using QuantiGene ViewRNA plate-based assay

• Biomarker support: analyzed plasma samples to support diabetes programs using C-peptide EIA, insulin EIA, glucagon luminex assay, SPE and Total Amide GLP-1 MSD assay, Active GLP-1 EIA, and Lincoplex PYY and GIP luminex assays

Scientist, Obesity, Department of Cardiovascular, Metabolic and Endocrine Diseases 2001-2008

• Developed a 384-well fluorescence polarization binding assay for 5HT2C receptor agonists screening; developed and executed a luciferase reporter assay to characterize leptinmimetics; implemented compound characterization using flow cytometry, ELISA, immunohistochemistry, Western blotting, and receptor binding assays

• Generated gene constructs and OBRb/IL6RE/luciferase, PYY, MTP transfected cell lines; performed OBRb-siRNA transfection; determined multiple genes expression using RT-qPCR

• Investigated leptin/insulin receptor signaling through the JAK/STAT3, MAPK, PI3K, AMPK, and PKC pathways, resulting two manuscripts

• Conducted in vivo studies of leptinmimetics in ob/ob and db/db mice, two lead structures identified

WASHINGTON UNIVERSITY IN ST. LOUIS, ST. LOUIS, MO

Research Scientist, Immunology Program, Department of Molecular Biology and Pharmacology 1991-2001

• Studied the mechanism and significance of antigen-stimulated apoptosis in the regulation of inflammation, and the mechanism of experimental autoimmune encephalomyelitis using in vivo and in vitro techniques including animal immunization, sampling and histological analysis, flow cytometry, cytotoxic assay, proliferation assay, apoptosis assays, cytokine bioassays, ELISA, RT-PCR, Western blotting, and immunohistochemistry

• Played a key role in the discovery of the role of Fas and Fas ligand in activation-induced death in lymphocytes

• Generated Th1 and Th2 cell lines and clones using helper T cell polarization and limiting dilution

EDUCATION

• M.S., Molecular Biology, Tongji Medical University, Wuhan, China

• M.B., Medicine, Tongji Medical School, Wuhan, China

PUBLICATIONS

1. McClure, K.F., Darout, E., Guimarães, C.R.W., DeNinno, M.P., Mascitti, V., Munchhof, M.J., Robinson, R.P., Kohrt, J., Harris, A.R., Moore, D.E., Li, B., Samp, L, Lefker, B.A., Futatsugi, K., Kung, D., Bonin, P.D., Cornelius, P., Wang, R., Salter, E., Hornby, S., Kalgutkar, A.S., Chen, Y. 2011 Activation of the G-protein coupled receptor 119: a conformation-based hypothesis for understanding agonist response; J. Med. Chem, 54: 1948-1952

2. Ruduan Wang and Andrew. G. Swick. 2009. Identification and characterization of a leptin-responsive neuroblastoma cell line. Biochem. Biophys. Res. Commun. 379: 835-839

3. Peter Cornelius, Eunsun Lee, Wen Lin, Ruduan Wang, Wendy Werner, Janice A. Brown, James G. Boyd and Kim McClure. 2009. Design, synthesis and pharmacology of fluorescently labeled analogs of serotonin: application to screening of the 5-HT2c receptor. J Biomol Screen 14 (4): 360-370

4. Shenoy, S., T.Mohanakumar, T.Chatila, J.Tersak, B.Duffy, R.Wang, A.R.B.Thilenius, and J.H.Russell.2001. Defective apoptosis in lymphocytes and the role of IL-2 in autoimmune Hematologic Cytopenias. Clinical Immunology. 99(2): 266

5. Thang Nguyen, R. Wang and J.H. Russell. 2000. IL-12 enhances IL-2 function by inducing CD25 expression through a p38 mitogen-activated protein kinase passway. Eur.J. Immunol. 30:1445

6. Wang, R., T. L. Ciardelli, and J. H. Russell. 1997. Partial signaling by cytokines: cytokine regulation of cell cycle and Fas-dependent, activation-induced death in CD4+ subsets. Cell. Immunol. 182:152

7. Wang, R., A. Rogers, Y. L. Ratliff and J. H. Russell. 1996. CD95-dependent bystander lysis caused by CD4+ T helper 1 effectors. J. Immunol. 157:2961.

8. Wang, R., A. Rogers, B. J. Rush and J. H. Russell. 1996. Induction of sensitivity to activation-induced death in primary CD4+ cells: a role for interleukin-2 in the negative regulation of responses by mature CD4+ T cells. Eur. J. Immunol. 26:2263

9. Wang, R., K. M. Murphy, D. Y. Loh, C. Weaver, and J.H. Russell. 1993 Differential activation of antigen stimulated-suicide and cytokine production pathways in CD4+ T cells is regulated by the antigen-presenting cell. J.Immunol.150:3832.

10. Russell, J. H., B. J. Rush, C. Weaver, and R. Wang. 1993. Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide. Proc. Nat. Acad. Sci. USA 90:4409.

11. Wang, R., S. I. Abrams, D. Y. Loh, C-S. Hsieh, K. M. Murphy, and J. H.Russell. 1993. Separation of CD4+ functional responses by peptide dose in Th1 and Th2 subsets expressing the same transgenic antigen receptor. Cell. Immunol. 148:357.

12. Russell, J. H., and R. Wang. 1993. Mutation uncouples suicide and cytokine pathways in mature T cells. Eur. J. Immunol. 23:2379.

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