Biochemist (Enzyme)/Enzyme Kinetics/ Food biochemistry/Fermantation

Mr. DEVENDRA KUMAR

Chemist, Alliance Enzyme Pvt. Ltd.

No. 13, Olpad, G. I. D. C., Opposite Surbhi Dairy, Surat Road Surat - Gujarat, India

Mb: +91-998*******(home), +91-966*******(office)

Tel.: +91-522-******* (Lab), Fax : +91-522-*******

E mail: w32hzw@r.postjobfree.com

Passport No: J2680938

http://www.researchgate.net/profile/Devendra_Kumar2/

Career Goal

A motivated research scholar with team spirit looking forward for a responsible and challenging position in any esteemed research and development group in corporate and industrial sector in order to utilize the knowledge, wisdom and expertise gained in my research career for human welfare.

Professional Qualification

Pursuing Ph.D (Pre-submitted) since 2009 in Botany with specialization in Biochemistry at Lucknow University, Lucknow and worked on thesis entitled ‘Biochemical characterization of cellulases by mango peel using microbial system (Aspergillus niger NAIMCC-F-02958). Registration number-923529 under guideship of Dr. Munna Singh (Professor), Div of Botany, Lucknow University, Lucknow and co guide Dr. (Mrs) Neelima Garg, Principal Scientist and Head, CISH, Lucknow.

M.Sc 2005 (Biochemistry) Biochemistry, Bundelkhand University, Jhansi.(Main Campus) SUB: Cell Biology, Genetics, Molecular Biology, Microbiology, Biotechnology, Animal biochemistry, Plant Biochemistry, Environmental biochemistry, Enzymology, Computer & Maths,

B.Sc 1999 (Biology) Zoology, Botany, Chemistry, DDU Gorakhpur University, Gorakhpur (Main Campus)

Research/Professional Experience: (25000/- + TA+ Accommodation)

i) Chemist, Alliance Enzyme Pvt. Ltd.(15 July-Till date) Working as chemist and in quality control under IP and USP reference standard and optimized R&D protocol and condition r of Pharmaceuticals Enzyme, Food Enzyme and Industrial Enzyme at commercial scale. Sampling of Raw materials, Testing of Raw/Packaging materials, Testing of Purified Water, In process quality testing of products, Preparation of Quality Control SOPs, Protocols etc, Preparation of QC reports, Qualification/Calibration of instruments/equipments, Able to Operate HPLC, UV-VIS Spectrophotometer, Refractometer, pH meter, Conductivity meter etc and Chemical analysis, Experience in Microbiological analysis or microbial load . SOPs preparation of chemicals/protocols for production and biochemical activities and maintain appropriate records of the daily activities.

ii) Senior Research Fellow, Food microbiology Div, CISH, (4August 2007-15July date 2012) worked as Senior Research Fellow in project entitled “Utilization of fruit processing waste for producing value added products” under “Application of microbiology for agriculture and allied sector” mega project funded by Indian Council of Agriculture Research (ICAR) in Central Institute for Subtropical Horticulture(CISH), Lucnow. I am working the following areas under this project: Screening of microorganism under GRASS status; Optimization of fermentation condition for maximum enzyme (Extracellular Amylase, Carboxymethyl cellulase (CMCase), β-glucosidase, Filterpaperase (FPase), Xylase, Polygalacturonase(PG), Pectinmethyl esterase (PME) production; Purification of enzyme protein by Ion-exchange chromatography, Size exclusion chromatography, Affinity chromatography, Biochemical characterization of desired enzyme; Immobilization by organic and inorganic matter and their application for juice clarification.

iii) Project Assistant-II,Plant Physiology & Biochemistry Div, NBRI, (8 April 2006-30 July2007) Council of Scientifc & Industrail Research, New Millennium Indian Technology Leadership Initiative (NMITLI) project titled “Genetic improvement of Jatropha curcus for Adaptation and oil yield” from National Botanical Research Institute (NBRI), Lucknow; Job profile included : Isolation and measurement of Metabolite, enzyme and Antioxidant; Photosynthesis and water relations in jatropha curcus plants. I have gained experience for handling of equipments like, UV spectrophotometer (96well) TECAN infinite-200, LICOR-6200 photosynthesis system and pressure chamber; Optimized condition of water use efficency

iii) Graduate trainee, Plant tissue Culture Div, CIMAP,(6 January2005-10 Jun 2005) M.Sc dissertation student in Central Institute of Medical & Aromatic Plant (CIMAP), Lucknow on Project entitled “Biotechnological studies for Micro propagation, Artificial seed production, Agrobacterium rhizogenes mediated Genetic Transformation & Photochemical analysis of some important MAP plant.

Technical Exterprise

Molecular biology and biochemical technique

Protein (Enzyme) metabolite & antioxidant isolation and purification, characterization, quantification.

Genetic transformation by Agrobacterium rhizogenes in some medicinal aromatic plant.

Isolation and screening of microorganism for maximum enzyme production, Pore plate technique, estimation of microbial lode in different food product.

Isolation of microorganism by pore-plate method and screened by enzyme assay method,

Carbohydrate including reducing sugar, total sugar, starch and fat quantification acidity from different food material like biscuit and fruit sample, dietary fiber extraction, moisture, ash, total soluble solid (TSS)

Quantitative measurement of chlorophyll, β-carotene, phaeophytin & anthosynin

Quantification of Vitamin C (Ascorbic acid) and β-carotenoid

Juice clarification by different enzyme treatment

Micro propagation& Artificial seed production.

Manually measurement of different soil parameter as VWC, GWC, Soil water holding capacity, permittivity (bo) and bulk density

Instrumentation expertise

Electrophoresis techniques: SDS-PAGE, 2-D PAGE, Isolelectric Foccusing, Silver staining;

Chromatographic techniques: Gel filtration, Gas chromatography, HPLC, HPTLC, BAS-CV, Mass Spectrometric techniques: LC-MS/MS, GC-MS;

Refractometer UV spectrophotometer (TECAN) Infinite-200(96well), Spectroquant NOVA 60, Double beam UV-VIS spectrophotometer (UV5704SS) Viscometer (BROOKFIELD)

Freeze drier (IISHIN)

WET sensor (HH-2)

WESCOR (Psyprometer)

Presser chamber (Soil moisture)

Porometer (Delta-T)

LICOR-6200

LICOR-1800

Publications:

Articles:

Devendra Kumar, Mohd. Ashfaque, Muthukumar. M, Munna Singh and Neelima Garg. Production and characterization of carboxymethyl cellulase from Paenibacillus polymyxa using mango peel as substrate. Journal Environmental Biology, 2012, 33(1): 81-84 http://www.jeb.co.in/journal_issues/201201_jan12/paper_13.pdf

Devendra Kumar, Kaushlesh K. Yadav and Munna Singh, Hydrolysis of wood saw dust by combined chemical pretreatment and enzymatic methods for lignocellulosic saccharification GERF Bulletin of Biosciences, 2011, 2(2):29-31 http://www.gerfbb.com/archivedetails.php?id=25

Devendra Kumar, Kaushlesh K. Yadav Munna Singh, Neelima Garg. Biochemical utilization of agro-industrial lignocelluloses rich waste for cellulase production. Research Journal of Agricultural Science: . 2012, 44 (2): 184-191 http://rjas.ro/index.php/rjas/article/viewFile/1466/1436

Devendra Kumar, Neelima Garg, M. Muthukumar and Kaushlesh, K. Yadav, Aonla pomace as substrate for endoglucanase production using Trichoderma harzianum, International Journal of Innovative Horticulture, 2012, 1(1):41-46. http://chai.org.in/2012/CHAI%20Journal%20Final%20(16-8-12).pdf

Devendra Kumar, M. Muthukumar, Neelima Garg. Utilization of Mahua Cake for cellulase production by using Trichoderma harzianum NAIMCC-F-02957 under Submerged Fermentation. Academic Journal of Plant Sciences, 5 (1) 01-06, 2012 doi:10.5829/idosi.ajps.2012.5.1.12, http://www.idosi.org/ajps/5(1)12/1.pdf

Devendra Kumar, Muthukumar M and Neelima Garg. Kinetics of extracellular α-amylase from Fusarium solani immobilized in calcium alginate beads. Journal Environmental Biology, 2012, MRN/648 Vol. 33(5/6) (Accepted). http://www.jeb.co.in/

Abstracts/Extended summary/Short papers:

Neelima Garg, Mohammad Ashfaque and Devendra Kumar, Production of α-amylase using mango kernel as carbon source by different amylolytic microbial isolates. Paper presented at 3rd Ind. Hort. Congress held at OUAT, Bhubaneshwar from 6-9 November 2008, pp.395

Neelima Garg, Devendra Kumar and Mohammad Ashfaque, Production and characterization of extracellular cellulases from a cellulolytic Bacillus sp. using mango peel as substrate. Paper presented at National Conference on Eco- friendly approaches in sustainable agriculture and Horticulture Production held at Amity University, Lucknow from 28-30 November 2008, pp.184

Neelima Garg, Devendra Kumar, Mohammad Ashfaque and Muthukumar.M., Paper presented on “Production of cellulase from mango peel residue left after pectin extraction using Aspergillus niger” National seminar on Production post harvest technology and marketing of mango organized Horticulture college and research institute, Periyakulam, 23-25September 2009, pp.215-217

Devendra kumar, Mohd. Ashfaque, Abhishek Shukla, Preeti Yadav, Jyoti Bajpai, Muthukumar. M and Neelima Garg, Poster presented on Optimization of condition for carboxymethyl cellulase production from mahua pomace using Trichoderma herzianum. National seminar on Food security and economic prosperity through processing & Preservation of food organized by Central Institute of Agriculture engineering (CIAE) Nabi Bagh, Berasia road, Bhopal(MP) 4-5, March 2010, pp.22

Devendra kumar, Mohd. Ashfaque, Abhishek Shukla, Muthukumar. M and Neelima Garg, Production,purification and characterization of α-amylase by Fusarium solani. using mango kernal as potential substrate National symposium on conservation Horticulture Organized by Indian society of horticulture research and development, Chaubatta, Almora, Uttarakhand, G.B Pant University of Agriculture & Technology, Pantnagar and Deportment of Horticulture and food Processing,Uttarakhand, Dehradun 21-23March 2010, pp.265

Neelima Garg, Preeti yadav, Devendra kumar and Mohd. Ashfaque Screening of bael varities for wine reparation National seminar on Food security and economic prosperity through processing & Preservation of food organized by Central Institute of Agriculture engineering (CIAE) Nabi Bagh, Berasia road, Bhopal(MP) 4-5 March 2010, pp.32

Neelima Garg, Preeti yadav, D.K Sukla, Sanjay Kumar, Mohd. Ashfaque, Devendra kumar and Jyoti Bajpai., Improved protocol for development of mango wine., Paper presented on National seminar on Biodiversity in mango for Sustainable Livelihood. Rehmankhera,P.O Kakori, Lucknow(P-9.1) 25-28 June, 2010, pp.98

Devendra Kumar, Kaushlesh K. Yadav, M. Muthukumar and Neelima Garg “ Property of calcium alginate immobilized corboxymethyl cellulase and β glucosidase produce by Aspergillus niger using mango peel”, paper presented at National conference on horti business linking farmer with market held a Swadesh prem Jagriti sangosthi 2011, Dehradum, Uttarakhand, from 28-31 May, 2011, pp.130.

Devendra Kumar, Kaushlesh K. Yadav, M. Muthukumar, Sanjay Kumar and Neelima garg “ mango juice clarification using purified fungal cellulase obtained from mango peel residue fermentation” paper presented at Global conference on Augmentation Production and Utilization of mango ; Biotic and Abiotic Stresses held a Central Institute For Subtropical Horticulture, lucknow, from 21- 24 June, 2011, pp.151.

Seminars/ symposia/conferences participation/presentations:

Participated in “International Conference on Advances in biomedical & Therapeutic Science”13-15th Jan 2005 Jhansi, Utter Pradesh.

Participated and presented poster entitled “Production and characterization of extracellular cellulases from a cellulolytic Bacillus sp using mango peel as substrate” in National Conference on Eco- friendly approaches in sustainable agriculture and Horticulture Production held at Amity University, Lucknow from 28-30 November 2008. pp.184

Participated and made oral presentation on paper entitled Production of Cellulase from mango peel residue left after pectin extraction using Aspergillus niger” National seminar on Production post harvest technology and marketing of mango organized Horticulture college and research institute, Periyakulam, September 2009. pp.215-217

Participated and presented poster on “Production, purification and characterization of β-amylase by Fusarium solani. using mango kernal as potential substrate” in National symposium on conservation Horticulture. Organized by Indian society of horticulture research and development, Chaubatta, Almora, Uttarakhand, G.B Pant University of Agriculture & Technology, Pantnagar and Dept of Horticulture and food Processing, Uttarakhand, 4-5 March Dehradun 2010. pp-265

Participated and presented poster on “Improved protocol for development of mango wine” in National seminar on Biodiversity in mango for Sustainable Livelihood. Rehmankhera,P.O Kakori, Lucknow(P-9.1) 25-28 June, 2010. pp.98

Participated and presented poster on entitled Property of calcium alginate immobilized corboxymethyl cellulase and βglucosidase produce by Aspergillus niger using mango peel in National conference on horti business linking farmer with market held a Swadesh prem Jagriti sangosthi 2011, Dehradum,Uttarakhand, from 28-31 may, 2011, pp.130.

Participated and presented poster on entitled “mango juice clarification using purified fungal cellulase obtained from mango peel residue fermentation” in Global conference on Augmentation Production and Utilization of mango ; Biotic and Abiotic Stresses held a Central Institute For Subtropical Horticulture, lucknow, from 21- 24 june, 2011, pp.151.

Awards

Awarded 2nd prize for poster paper entitled “Production and characterization of extracellular cellulases from a cellulolytic Bacillus sp. using mango peel as substrate” at National Conference on Eco- friendly approaches in sustainable agriculture and Horticulture Production held at Amity University, Lucknow from 28-30 November 20

Awarded 3rd prized for the best oral presentation entitled “Production of cellulase from mango peel residue left after pectin extraction using Aspergillus niger” in National symposium on production,post harvest technology and marketing of mango organized by the deportment of fruit crops,Horticulture college and research institute, Periyakulam 23-25 September 2009

Awarded 2nd prize for best poster paper entitled ‘Optimization of condition for carboxymethyl cellulase production from mahua pomace using Trichoderma herzianum’ at National Conference on “Foods” security and economic prosperity through processing & Preservation of food organized by Central Institute of Agriculture engineering (CIAE) Nabi Bagh, Berasia road, Bhopal(MP) 4-5 Feb 2010

Awarded the best poster paper entitled “Production, purification and characterization of β- amylase by Fusarium solani. using mango kernel” as potential substrate’ at National Conference on “National symposium on conservation Horticulture Organized by G.B Pant University of Agriculture & Technology, Pantnagar and Dept of Horticulture and food Processing, Uttarakhand 21-23 March Dehradun

Awarded the best poster paper on ‘Improved protocol for development of mango wine’, Paper presented on National seminar on Biodiversity in mango for Sustainable Livelihood. Rehmankhera, P.O.Kakori, Lucknow (P-9.1) 25-28 June, 2010, pp-98

Patents

Two entitled has been submitted in the patent office New Delhi.

Neelima Garg, Abhishek Shukla, Preeti Yadav, Mohd. Ashfaque, Devendra Kumar and Jyoti Bajpai. A biscuit composition enriched with dietary fibre extracted from mango processing waste of finisher stage and a process for preparation of the same. Application no. 510/ DEL/2011.

Neelima Garg, Mohd. Ashfaque, Abhay Dixshit, Devendra Kumar, Sanjay Kumar, Preeti Yadav, Abhishek Shukla and Jyoti Bajpai. A low energy process for extraction of fibre from mango processing waste of finisher stage. Application no. 511/DEL/2011.

Additional Information:

Computer Skills

Working knowledge and expertise in Windows, MS-WORD, MS-EXCEL, MS Power Point, Photo Adobe Shop, Corel Draw 12 Ability to independently learn and use any software for solving biological problems.

Statistical skills

(i) DOE++: Software Tool for Experiment Design and Analysis (DOE) of experiment design types, including factorial designs, fractional factorial designs, Taguchi robust designs, response surface method designs and reliability DOE.

(ii) SigmaPlot and SYSTAT enterprise level data management solutions for application areas such as genomics, proteomics, chemical databases

Teaching Experience

Helped two MSc. (Biotech) students for summer training under AMAAS project (2007-2008) and two more B. Tech students students (2009-20010) under the same project to enhance their technical skills and scientific knowledge.

Personal Profile

Father’s name Sh. Udai Bhanu Choudhary

Date of birth 06th August 1979

National Indian

Language Proficiency English, Hindi

Interpersonal Skills: Hardworking, Determination, Dedicated, Initiator, Confident.

Postal Address 3/76 Vijyant Khand, Gomati Nagar, Lucknow -226010

Detail of References

Dr. Neelima Garg

Principal scientist & Head of deportment

Food Microbiology Lab,

Post Harvest Management division

Central Institute for Subtropical Horticulture (CISH), Lucknow

Telephone -+91-941*******

email w32hzw@r.postjobfree.com,

Shri. Muthukumar, M.

Scientist

Biotechnology Lab,

Crop Improvement division,

Central Institute for Subtropical Horticulture (CISH), Lucknow

e-mail w32hzw@r.postjobfree.com

Dr. U.V Pathra

Scientist –F

Plant physiology & Biochemistry

National Botanical Research Institute (NBRI)

Lucknow -226001

e-mail w32hzw@r.postjobfree.com, Telephone: +91-941*******

Dr Suchitra Banarjee

Scientist E-II

Plant tissue culture division (PTC)

Central Institute of Medical & Aromatic Plant (CIMAP)

Lucknow -226001

e-mail w32hzw@r.postjobfree.com, Telephone: 206-***-****

Dr. Munna Singh

Reader

Deportment of Botany

Lucknow University, Lucknow

e-mail w32hzw@r.postjobfree.com, Telephone: +91-945*******

Declaration

I hereby declare that the above information is true to best of my knowledge and there is no discrepancy.

Date:

Place: DEVENDRA KUMAR

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