Cindy (Xinrong) Wang, Ph.D.

**** ********* ***** ** | Mt Pleasant, SC 29466 | 919-***-**** | w2nhvi@r.postjobfree.com

Experience

Medical University of South Carolina, Department of Surgery – Charleston, South Carolina

Research Technologist III, 2010 to Present

Managed projects focused on molecular therapy in murine models of pancreatic cancer and molecular mechanism by which CaSm overexpression leads to pancreatic oncogenesis. Research Project Highlights:

• Murine models of pancreatic cancer (intrasplenic, intrapancreatic, and subcutaneous routes) in immuno-competent (C57BL6 and diet-induced obese model) and immuno-compromised mice (NSG and nude mice) to generate localized and metastatic pancreatic cancer models, nanoparticle-mediated P53 gene delivery

• In vivo and ex vivo imaging (BLI, fluorescence, ultrasound)

• Performed assays to evaluate endogenous and transgene expression by Western blots, IFA, and qPCR

• Bacteria culture, purification of recombinant proteins, and preparation of plasmid DNA (1-10 mg)

• Cell culture, transfection, cell proliferation and viability assays, siRNA/lentivirus shRNA gene knockdown, development of 5FU and gemcitabine resistant cells

• SPPS of transferrin peptides, Fmoc chemical synthesis techniques, C18 RP-HPLC, and mass spectrometry

• Data analysis, preparation of manuscripts and NIH funding progress reports

• General lab management and training of lab technicians

GenPhar, Inc (a biotech startup company in the R&D of prophylactic and therapeutic vaccines against viral infections) – Mt. Pleasant, South Carolina

Senior Scientist, 03/2010 to 10/2010; 02/2012 to 07/2012

Lead molecular biology group and managed projects, focused on immunoassay of vaccine antigens, construction of viral vectors, and cell line development. Research Project Highlights:

• Screened adenoviral vaccines by immunoassays, DNA sequencing, and PCR. Selected clones for large scale production

• Designed assays to detect and quantitate replication competent viral vectors

• Directed R&D effects by using ProVax and TheraVax vaccine platforms. Constructed and isolated recombinant Ad5 adenoviral and modified vaccinia virus Ankara (MVA) vectors

• Bacteria culture, titer, and propagation/purification of viruses

• Developed and characterized adenovirus E1 transformed human lung primary cell line for the production of RCA-free adenoviral vaccines

Mycobacteriology Research Laboratory, William S. Middleton Memorial Veterans Hospital – Madison, Wisconsin

Research Biologist, 2003 to 2010

• Generated insertion mutant library in Corynebacterium matruchotii by Tn-5 transposon mutagenesis

• Generated knockout mutants in Mycobacterium smegmatis by transduction of wt strain with knockout construct packaged mycobacteriophage

• Isolated and characterized cell wall mycolates and corynomycolates as well as novel glycolipids in mycobacteria and corynebacteria by column chromatography, TLC, and mass spectrometry

• Synthesis of fatty acids and derivatization of fatty acids and mycolic acids; preparation of diazomethane

• Supervised lab technicians and managed routine lab maintenance

Medical University of South Carolina, Department of Biochemistry and Molecular Biology – Charleston, South Carolina

Research Instructor, 1999 to 2003

• Examined the effects of adenovirus-mediated prostasin gene delivery in cardiovascular and renal diseases

• Generated and characterized prostasin transgenic and knockout mouse lines

• Supervised lab technicians and managed routine lab maintenance

Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina

Postdoc Fellow, 1995 to 1999

• Constructed and purified Ad5 recombinant adenovirus expressing human and rat prostasin

• Investigated the effects of adenovirus-mediated gene delivery in hypertensive animal models

• Examined gene expression and regulation of kallikrein-kinin system

• Purified and characterized rat tissue kallikreins (rat prostate kallikrein, tonin, T-kininogenase, and tissue kallikrein)

Education

Medical University of South Carolina – Charleston, South Carolina

PhD in Biochemistry and Molecular Biology, 1995

Peking University – Beijing, P. R. China

MS in Biochemistry, 1989

BS in Biochemistry, 1985

Research Fellowship

Medical University of South Carolina, Health Science Foundation Award, 2000-2001, $10,000

“The role of prostasin in salt sensitive hypertension”

Principal Investigator

Publications

1. Wang C, Tang C, Chao L, Chao J: Biochemical characterization and substrate specificity of rat tissue kallikrein (S3): Comparison with tissue kallikrein, tonin and T-kininogenase. Biochim Biophys Acta 1121:309–316, 1992.

2. Chagas JR, Hirata IY, Juliano MA, Xiong W, Wang C, Chao J, Prado E: Substrate specificities of tissue kallikrein and T-kininogenase: Their possible role in kininogen processing. Biochemistry 131:4969–4974, 1992. PMID: 1599922

3. Wang C, Chao L, Chao J: Direct gene delivery of human tissue kallikrein reduces blood pressure in spontaneously hypertensive rats. J Clin Invest 95:1710–1716, 1995. PMCID: PMC295685.

4. Wang C, Chen YP, Chao L, Chao J: Regulatory elements in the promoter region of renal kallikrein gene in normotensive vs. hypertensive rats. Biochem Biophys Res Commun 217:113–122, 1995.

5. Garrett JR, Chao J, Proctor GB, Wang C, Zhang XS, Chan K-M, Shori DK: Influences of secretory activities in rat submandibular glands on tissue kallikrein circulating in the blood. Exp Physiol 80:429–440, 1995.

6. Chao C, Wang C, Madeddu P, Liang Y, Chao L, Chao J: Differential regulation of kallikrein, kininogen and kallikrein-binding protein in hypertensive rats. Am J Physiol 271:F78–F86, 1996.

7. Madeddu P, Parpaglia PP, Anania V, Glorioso N, Chao C, Wang C, Chao J: Sexual dimorphism of cardiovascular responses to early blockade of bradykinin receptors. Hypertension 27 (Part 2):746–751, 1996.

8. Wang C, Chao C, Chen L-M, Chao L, Chao J: High salt diet upregulates kininogen and downregulates tissue kallikrein expression in Dahl-SS and SHR rats. Am J Physiol 271:F824–F830, 1996.

9. Wang C, Chao C, Chao L, Chao J: Expression of human tissue kallikrein in rat salivary glands and its secretion into circulation following adenovirus-mediated gene transfer. Immunopharmacology 36:221–227, 1997.

10. Wang C, Ford P, Chao C, Chao L, Chao J: Effect of cyclosporin A on the expression of tissue kallikrein, kininogen, and bradykinin receptor in rat. Am J Physiol 273:F783–F789, 1997.

11. Yayama K, Wang C, Chao L, Chao J: Kallikrein gene delivery attenuates hypertension, cardiac hypertrophy and enhances renal function in Goldblatt hypertensive rats. Hypertension 31:1104–1110, 1998.

12. Wang C, Chao C, Madeddu P, Chao L, Chao J: Central delivery of human tissue kallikrein gene reduces blood pressure in hypertensive rats. Biochem Biophy Res Commun 244:449–454, 1998.

13. Chao J, Wang C, Chao L. Gene therapy for hypertension: a review of potential targets. BioDrugs 11:43-53, 1999.

14. Murakami H, Yayama K, Miao RQ, Wang C, Chao L, Chao J: Kallikrein gene transfer inhibits vascular smooth muscle cell growth and neointima formation in the rat artery after balloon angioplasty. Hypertension 34:164–170, 1999.

15. Zhang JJ, Wang C, Lin K-F, Chao L, Chao J: Human tissue kallikrein attenuates hypertension and secretes into circulation and urine after intramuscular gene delivery in hypertensive rats. Clin Expe. Hypertension 21:1145–1160, 1999.

16. Dobrzynski E, Wang C, Chao J, Chao L: Adrenomedullin gene delivery attenuates hypertension, cardiac remodeling and renal injury in deoxycorticosterone acetate-salt hypertensive rats. Hypertension 36:995–1001, 2000.

17. Wang C, Dobrzynski E, Chao J, Chao L: Adrenomedullin gene delivery attenuates renal damage and cardiac hypertrophy in Goldblatt hypertensive rats. Am J Physiol 280:F964–F971, 2001.

18. Chao J, Kato K, Zhang JJ, Dobrzynski E, Wang C, Agata J, Chao L: Human adrenomedullin gene delivery protects against cardiovascular remodeling and renal injury. Peptide 22:1731–1737, 2001.

19. Chao J, Wang C, Chao L: Adenovirus-mediated gene transfer for cardiovascular and renal diseases. Methods Enzymol 346:247–263, 2002.

20. Wang C, Chao J, Chao L: Adenovirus-mediated human prostasin gene delivery stimulates aldosterone production and causes hypertension in rats. Am J Physiol 284:R1031–R1036, 2003.

21. Takayama K, Wang C, Besra GS: Pathway to synthesis and processing of mycolic acids in Mycobacterium tuberculosis. Clin Microbiol Rev 18:81–101, 2005.

22. Wang C, Hayes B, Vestling MM, Takayama K: Transposome mutagenesis of an integral membrane transporter in Corynebacterium matruchotii. Biochem Biophys Res Commun 340:953–960, 2006.

23. Chen LM, Wang C, Chen M, Marcello MR, Chao J, Chao L, Chai KX. Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation. Am J Physiol Renal Physiol 291:F567-F577, 2006.

24. Mishra AK, Alderwick LJ, Rittmann D, Wang C, Bhatt A, Jacobs WR Jr, Takayama K, Eggeling L, Besra GS. Identification of a novel alpha(1-->6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant. Mol Microbiol. 68:1595-1613, 2008.

25. Wang C, Mahrous EA, Lee RE, Vestling MM, Takayama K. Novel Polyoxyethylene-containing glycolipids are synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis cultured in the presence of Tween 80. J Lipids. 2011;2011:676535.

26. Little EC, Wang C, Watson PM, Watson DK, Cole DJ, Camp ER. Novel immunocompetent murine models representing advanced local and metastatic pancreatic cancer. J Surg Res 176(:359-366, 2012.

27. Findlay VJ, Moretz RE, Wang C, Vaena SG, Bandurraga SG, Ashenafi M, Marshall DT, ER. Slug expression inhibits Vitamin D-mediated sensitivity to radiation in colorectal cancer. CBT, submitted.

28. Camp ER, Wang C, Little EC, Watson PM, Pirollo KF, Rait A, Cole DJ, Chang EH, Watson DK. Transferrin receptor targeting nanomedicine delivering wild type p53 gene sensitizes pancreatic cancer to gemcitabine therapy. Manuscript in preparation.

29. Camp ER, Hurst K, Wang C, Watson DK. Optimizing gene silencing strategies for pancreatic cancer. Manuscript in preparation.

Honors

Student Research Day Award, 1996

Medical University of South Carolina, Charleston, SC

Dean’s Award, College of Graduate Studies, 1996

Medical University of South Carolina, Charleston, SC

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