analytical chemist

ANALYTICAL SKILLS

Analytical Procedures and Equipment: Electrophoresis, RT-PCR, UV-vis, SDS-Page, DNA Sequencer(1998), Capillary Gel Electrophoresis, Conductivity, pH, amino acid analyzer, Reverse Phase-HPLC, Tryptic peptide map, Methionine Oxidation Peptide map, Size Exclusion, Glycan, AccQ-tag

Regulatory: Experience working in the following lab settings: cGMP, GLP, ISO9001, ICH and EPA

Software: Empower II, 32 karat, Microsoft Office, Notes, Atlas and ChemStation , LIMS

Certificates: HPLC and GC/MS Certificate Dr. Marvin McMasters at University Missouri St. Louis, HPLC Methods Development Certificate from Training Masters by Dr. Raymond Wong, Introduction to Molecular Cloning sponsored conducted by Dr. Talbot at Duke University

Experience:

Associate Scientist/Analytical Chemist March 2009-September 2009 Pfizer, Global Biologics Research and Development, Chesterfield, MO Contract completed

Perform Peptide Mapping, Glycan, Size Exclusion, and UV-vis Analysis of Proteins/MAbs. Assisted in Methods Validations. Wrote a BLA(Method summary). Performed a SEC Comparability Study. Revised SEC method. Prepared slides on Powerpoint for project review. Training of Colleagues from Belgium, HPLC, SOPs, Capillary Gel Electrophoresis training, balance, pH meter, and Troubleshooting HPLC system. Maintain a research notebook with detailed notes on the experiments for repeatability. GMP and GLP notebook and data documentation. In charge of the SEC analysis for project I was working on.

Laboratory Technician Cascade Lab November 2008-December 2008 Proctor &

Gamble, St. Louis, MO Contract completed Analyzed powder for pH. Prepared samples for analysis,Titrations. Maintained GLP documentation records for sample testing. Retrieved samples from manufacturing.

Biochemist I Protein Analytics Department June 2008-September 2008 Monsanto,

St. Louis, MO Contract completed Analyzed proteins for amino acid content by using Waters AccQ-Tag method of HPLC Analysis. Analyzed proteins for amino acid content by using a Hitachi

Amino Acid Analyzer. Analyzed results via Microsoft Excel. Maintained GLP and GMP documentation for samples in research notebook. LIMS

QC Laboratory Technician, March 2008-May 2008, Evonik Stockhausen, Greensboro,

NC Contract completed Wet bench chemistry: Performed Gel bed analysis on superabsorber. Analyzed superabsorber for Absorbance Under Load (AULs) and Teabag analysis of absorbance

ability, ball mill, Density, metal detection, and sieve. Responsible for ensuring

manufacturing gets transmitted the results by SAP transfer. Retesting every hour if

results do not meet specifications to help manufacturing find the problem in the lines. GLP Documentation of data.

Chemistry Lab Associate, November 2007-January 2008, Catalent, Morrisville, NC lay-off. Prepared Validation Standards and samples for HPLC. Assisted with Stent analysis by HPLC. Prepared mobile phase prep for HPLC analysis. Performed Appearance testing, Reviewed data. Review notebooks of colleagues and maintain GLP/GMP documentation of tests.

Sample Prep Technician (Funded) (May, 2007 to June, 2007) CompuChem Cary, NC

Funded. Project dependant.Extracted Pesticides and herbicide from waste water

samples for GC analysis. Use of separatory funnels for extraction. Performed

Biochemical Oxygen demand analysis. Assisted with Cyanide analysis. GLP data

documentation protocols.

Laboratory/Biochemist (March 2004 to October 2004) North Carolina State University,

Raleigh, NC (Grant Funded)Troubleshot SDS-PAGE procedures for protein analysis of soil samples, Setup standardized forms for analysis. Tutored students in Biochemistry, graded quizzes and tests. GLP Data Documentation.

QC Lot release Specialist (October 2002 to May 2003) Diosynth-RTP, RTP, NC

Permanent position—Lay-off. Documented data results into Excel Spreadsheet. Filed

Batch records, and QC Sheets as data was received. Audited Batches to ensure that all

documentation was present. Submit the lot to Quality Assurance for final disposition for

release. GMP Data documentation

QC Associate (January 2002 to October 2002) Diosynth-RTP, RTP, NC. Permanent

Promoted to QC Lot release Specialist. Preformed RP-HPLC analysis of fermentation

product. Analyzed protein samples for concentration by UV-vis. Invented a way to save

a batch. GMP documentation of analysis on appropriate forms. Initiated a gravimetric

UV-vis analysis of protein samples. LIMS.

Manufacturing Associate (February 2000 to January 2002) Diosynth-RTP/Covance,

RTP, NC. Permanent Audit documentation for lots and corrected errors. Run Centrifuges, filtration using Pall filtration apparatus. GMP documentation of extraction of the drug product from fermentation product. Purchase and assembled mobile lab bench for recovery 1 for placement of UV-vis Spectrophotometer. Assisted Management with Cleaning

Validiation and Stability testing. Performed sample Loggin.

• Career started in 1993, after 1996 career history was contract laboratory positions in Missouri. Most of the positions were wet bench analysis with one HPLC position and one DNA sequencing position. I worked in Nuclear Medicine in the manufacturing area where one had to assemble the file lines.

EDUCATION:

University Of Missouri,Saint, Louis, MO, Bachelor of Science in Biology 1997

Lewis and Clark Community College, Godfrey, IL , Associate of Applied Science 1989

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