Development Manager
Resume:
Stacy Carlucci-Derbyshire
Warminster, PA
*************@*****.***
SUMMARY:
Research Scientist with 7 years of drug discovery research experience in the biotechnology field. Skilled in providing assay development and pharmacology support to identify and evaluate the activity of molecules on target of interest.
SKILLS:
• Assay Development
• Automated liquid handling
• ELISA
• [35S]GTP S binding
• FLIPR
• HTS
• Drug discovery
• Radioisotope handling
• Cell Culture
• TR-FRET
• GraphPad Prism, CeuticalSoft, Microsoft Excel and PowerPoint
EDUCATION:
Rutgers University, Bachelor of Science in Biology, 2003
George H. Cook Scholar
EMPLOYMENT HISTORY:
Merck Research Laboratories, North Wales, PA
2009-Present Research Associate (Contract Position)
• Assay development and uHTS of GPR luminescence assay using PathHunter™ enzyme fragment complementation technology.
• Assisted with the pharmacological profiling of several orphan GPCR's using PathHunter™, Lance, and Ca2+ flux assays.
• Miniaturization of cell based assays from 384 well to 1536 and 3456 well format.
• Assisted in the development of an HTRF (InFret) strand transfer assay.
• Received training to operate the Biomek® FX, Beckman BioRAPTR FRD™ Workstation, CyBio CyBi-Well, GNF Pintool and GNF Bottle Valve automated liquid handling systems.
• Performed cell based and biochemical luminescence assays using the Wallac ViewLux microplate imager.
• Provided automated cell culture support for assay development and screening needs. Ability to multitask support for several scientists at once throughout all stages of development of assays and execution of screens.
Redpoint Bio Corporation (formerly Linguagen Corporation), Ewing, NJ
2008-2009 Senior Research Associate
2005-2008 Research Associate
• Whole cell Ca2+ flux and Membrane Potential assays using 384-well FLIPRTetra
• HTS and pharmacological profiling of TRPM5 channel modulators to support SAR effort
• Performed selectivity assay using TRPM4b, TRPV1, and TRPA1 ion channels to evaluate HTS hits and leads
• Investigated zero-efficacy TRPM5 ligands for use as pharmacological tools to study mechanism of action of TRPM5 inhibitors (negative-efficacy ligands).
• Assisted in the training of new Research Associates
• Contributed to the development and management of an electronic compound storage system
• Data analysis using CeuticalSoft and GraphPad Prism
• Provided cell culture support as needed (HEK, CHO)
• Results contributed to the publication of one book chapter and two peer-reviewed journal articles. One manuscript is in progress.
Rutgers University, New Brunswick NJ
2004-2005 Laboratory Researcher
• Performed colorimetric biochemical assays to measure levels of oxidative stress markers such as Lipid Hydroperoxide, Glutathione, Glutathione Peroxidase, and Creatine Kinase using SPECTRAmax® plate reader
• ELISA of 8-Isoprostane levels in human plasma
• Supervision and instruction of student research projects
• As laboratory manager:
Placed orders for instruments, chemicals, and supplies.
Performed instrument maintenance, sample storage, and laboratory organization.
• Assisted in the writing of publications
Linguagen Corporation, Cranbury, NJ
2003-2004 Research Associate
• Participated in HTS effort to identify compounds that effect GPCR signaling pathway.
• Pharmacological characterization of GPCR antagonists using a [35S]GTP S binding assay
• Assay development- converted [35S]GTP S filtration-based assay to a Scintillation Proximity Assay (SPA).
• Monitor usage and disposal of radioactive or biohazard compounds and waste
• Supervision and instruction of laboratory technicians
• Maintenance, ordering, and inventory of lab equipment, instruments, and consumables
• Certification in NRC and OSHA guidelines for proper laboratory conduct and safety, including 40 hours of RSO training at Radiation Safety Academy, Gaithersburg, MD
• HPLC using anion exchange column to evaluate the stability of Adenosine 5’ Monophosphate
Nelson Laboratories and the Environmental and Occupational Health Sciences Institute. Rutgers University, New Brunswick, NJ
2002-2003 Laboratory Assistant
• Analysis of biological samples with atomic absorption spectroscopy
• Collection of shorebird, pine snake, and water snake data, contributing to two peer-reviewed publications
• Digestion of samples for analysis of heavy metals and mercury
• Preparation of analytical standard solutions and various reagents for laboratory research
• Conducted public surveys regarding metal contaminants in fish, and Department of Energy Superfund site cleanup
PUBLICATIONS:
Burger J, Jeitner C, Jensen H, Fitzgerald M, Carlucci S, Shukla S, Burke S, Ramos R, Gochfeld M. Habitat use in basking Northern water (Nerodia sipedon) and Eastern garter (Thamnophis sirtalis) snakes in urban New Jersey Urban Ecosystems, 2004;7(1): 17-27.
Burger J, Carlucci SA, Jeitner CW, Niles L. Habitat choice, disturbance, and management of foraging shorebirds and gulls at a migratory stopover. Journal of Coastal Research, 2007; 23(5): 1159-1166.
Bryant, R.W., Atwal, K.S., Bakaj, I., Buber, M.T., Carlucci, S., Cerne, R., Cortes, R., Devantier, H.R., Hendrix, C.J., Lee, S.P., Palmer, R.K., Wilson, C., Yang, Q., Salemme, F.R. Sweetness Enhancement. In: Sweetness and Sweeteners: Biology, Chemistry and Psychophysics (ed. D.K. Weeasinghe and G.E. Dubois), Vol. 979, pp. 386-399. Oxford University Press, Washington, DC.
Devantier HR, Long DJ, Brennan FX, Carlucci SA, Hendrix C, Bryant RW, Salemme FR, Palmer RK. Quantitative assessment of TRPM5-dependent oral aversiveness of pharmaceuticals using a mouse brief-access taste aversion assay. Behavioral Pharmacology, 2008;19(7):673-82.
Williams CA, Carlucci SA. Oral vitamin E supplementation on oxidative stress and antioxidant status in intensely exercised horses. Equine Veterinary Journal Supplement, 2006; (36):617-21.
Palmer RK, Atwal KS, Bakaj I, Carlucci S, Buber MT, Cerne R, Cortes R, Devantier HR, Hendrix C, Jorgensen V, Lee SP, Pawlyk AC, Sprous DG, Zhang Z, and Bryant R, Triphenylphosphine oxide (TPPO) is a potent and selective inhibitor of the transient receptor potential M5 (hTRPM5) channel. Manuscript in preparation.
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