Research Assistant/ Analyst

MEGHA KAMATH

***N **th Street, Philadelphia, PA *9104. Ph 267-***-****

*****.******@*****.***

Objective: Research Assistant/Analyst

Laboratory Skills

• Flowcytometry and Hemocytometry

• UV-visible spectrophotometery

• Affinity, size exclusion and ion exchange chromatography

• Gel electrophoresis and western blotting

• Genomic mapping, RT-PCR, shot-gun cloning and gene transformation

• Cell plating

• Making Agar plates

• SDS-PAGE, RT-PCR, ELISA

• Material testing

• Biomaterials

• ECG, EKG, EOG

• Conductivity and pH meters

• Fabrication of micro and nanoparticles

• Google Sketchup

• MATLAB

• LaTeXe

• Endnote X5

• Engauge Digitizer

• Mimics

• Cortex Motion Analysis

• Microsoft Office (Word, Excel, PowerPoint)

• Computer programming (C++)

Education

Drexel University, Philadelphia, PA

GPA: 3.26

Bachelor of Science in Biomedical Engineering, Tissue Engineering

June, 2011

Research Project Experience

Quantification of the mRNA and functional protein levels of IL-8 chemokines

• Stimulated human peripheral mononuclear blood cells (PMBCs) using lipopolysacchrides (LPS)

• Utilized the ELISA assay and RT-PCR screening to obtain mRNA and functional protein levels of IL-8 chemokines

Performed research and development on alternative approaches to present day organ transplantation treatment

• Constructed National Institute of Health (NIH) proposals for liver, heart and pancreas regeneration in vitro employing molecular signaling pathways, stem cells and bio-reactors

Designed a non-invasive research instrument for the early detection of atherosclerosis by testing for the best prototype

• This tool uses infra-red technology to determine the pulse wave velocity of the radial artery.

• The resultant data, along with the Moens’ Korteweg equation, was used to obtain the elasticity of the radial artery

Research Experience

Drexel University November 2011- May 2012

Researcher under Dr. Balasubramanian

• Contribute to the quantification of the spine and thoracic cage structure and its correlation to pulmonary function in scoliosis patients with the help of Engauge Digitizer.

• Use Mimics to quantify vertebral sections of the spinal cord for cross comparison of scoliosis between species

• Use Cortex Motion Analysis to analyze the various effects of the seat-belt system on children seated in the car

BARD Holding, Inc March-September 2011

Research and development-Technical Assistant

• Analyzed algal cultures using the Millipore Guava easyCyte FlowCytometer for lipids and chlorophyll identification

• Engineered the process flow for optimum algal growth and high lipid content of algae

• Constructed quality protocols and standard operating procedures for the proper application of enzymatic reagents, algal media, instrumentation and maintenance of system components

National Center for Biological Sciences, TIFR Bangalore, India

December 2009-March 2010

Research trainee under Dr. Obaid Siddiqi in Behavioral and Neurobiology

• Researched navigation methods of Drosophila melanogaster larvae, for comparison when presented with ethyl acetate

• Developed tracks with MATLAB to study parameters among every larva for comparison in different odor concentrations

• Modeled a real time system for the behavior of larvae in their natural environment.

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