senior histologist

CURRICULUM VITAE

Ma, Minhui

**** ******** **.

Lilburn, GA 30047

Tel: 770-***-****

Email: *******@*****.***

Citizen : U.S.

EDUCATION and CERTIFICATION

HTL (ASCP), 2010

Certificate of DAKO Immuno-Autostainer training DAKO, USA 2005

M. Sc. Cellular and Molecular Biology, Henan Medical University, China, 1990

Certificate of Histology & Embryology, Shanghai Medical University China, 1983

M. D. Henan Medical University, China, 1982

TECHNIQUES & SKILLS

HistoPathology & Imaging:

Perform histopathological techniques including patients and animal biopsy/tissue grossing, fixation, processing, paraffin embedding, sectioning as well as frozen tissue preparing and cryotomy. Cytospin and blood smear preparation. H.E. and special stains, Immunocyto/histochemical and Immunofluorescent staining. In situ hybridization. HE and IHC/ Immunofluorescent staining with Leica and Dako Autostainers. Troubleshooting, optimizing histology and immunohistochemistry procedures.

Maintain laboratory equipments as tissue processor, microtome, cryostat and repository of slides; prepare solutions, oversee technical processes and train new user; hazardous materials procedures; implement new methods.

Knowledge of tissue morphology and experience of teaching Histology & Embryology for medical students. Image analysis (e.g., ImageJ, Image Pro Plus) of light and electron microscopy and confocal microscopy. Responsible for keeping data records, primary data analysis, and assisting manuscript preparation, including photo imaging and figure plate editing.

Cell biology & Molecular biology:

Cell culture and primary cell isolation from animal and human tissue. DNA, RNA and protein isolation, PCR and RT-PCR. SDS-PAGE and Western Blotting. Flow Cytometry. BSL3 lab experiences with HIV and VSV-G virus products. TCID50 assay and ELISA.

Monoclonal antibody techniques including immunization of animal, preparation of spleen cell suspension, fusion of myeloma cells with immune spleen cells, cloning of hybridoma cell lines. Establishment of experimental models of transplanted carcinoma in nude mice and animal surgery. Genotyping.

Management:

Order supplies, equipment, and lab maintenance & organization. Monitors quality control of reagents and cleaning procedures and addresses any problems. Keeps abreast of safety procedures and monitors the handling, storage and disposal of hazardous substances. Train and oversee other laboratory postdoctoral fellows, graduate students and research staffs and supervise laboratory daily running.

WORK EXPERIENCE

2011 – 2012, GSU, CIII Senior Histopathologist

1997 - 2011, Emory Univ. School of Medicine

Dept. of Medicine, Research Specialist, lead

Dept. of Pediatrics Infectious Diseases, Supervisor Research Specialist, lab manager

Dept. of Biomedical Engineering and Vascular Surgery, Associate Research Professor

Dept. of Pharmacology, Postdoctoral fellow

* 2005 - 2006, Emory Univ. School of Public Health

Dept. of Epidemiology, Histotechnologist II, Lab manager

1982 - 1996, Lecturer, Assistant, Associate Professor of Histology & Embryology

Dept. of Histology and Embryology, Henan Medical University, China

AWARDS

1995 - 1998: Excellent Young Teacher (Professor) Award, Henan Province Educational

Committee (an honor given for three years; includes special support & funding)

1998: Excellent Book Award, Henan Province Educational Committee

1996: Award for Achievement in Science & Technology (Medicine & Health),

Henan Province Health Department

1996: Five Publications Selected for Excellent Academic Publication

Awards, Henan Province Scientific Committee

1996: University Audio-Visual Teaching Accomplishment Award of Excellence,

Henan Province Educational Committee

1996: Award of Young Professors Teaching Competition of Henan Medical University.

SELECTED PUBLICATIONS

1. Shiqin Xiong, Gloria Salazar, Alejandra San Martin, Mushtaq Ahmad, Nikolay

Patrushev, Lula Hilenski, Rafal Robert Nazarewicz, Minhui Ma, Masuko Ushio-Fukai and R. Wayne Alexander. PGC-1α serine570 phosphorylation and GCN5-mediated acetylation by Angiotensin II drive catalase downregulation and vascular hypertrophy JBC.M109.065235

2. Monica T. Hinds1, Min-hui Ma, Ann E. Ensley, Stephanie M. Kladakis, Keri Vartanian1, Robert M. Nerem, and Steve Hanson. Potential of Baboon Endothelial Progenitor Cells for Tissue Engineered Vascular Grafts, Journal of Biomedical Materials Research Part A 804-812, 27, 2007

3. Ma, Min-hui and Hanson, S.R. Circulating endothelial progenitor cells from non-human primates. Submit and present at International Society for Stem Cell Research 1st Annual Meeting. Washington, D.C. June 8-11, 2003

4. Ma, Min-hui and Hanson, S.R. Circulating endothelial progenitor cells from baboon blood. Submitted and presented at 42nd The American Society for Cell Biology Annual Meeting. San Francisco, U.S.A. Dec. 14-18, 2002

5. Kladakis, Stephanie M., Ma, MH, Duvall, C.L., Bronnenberg, D., Nerem, R.M. and Hanson, S.R. The effect of shear stress on the cell shape and F-actin alignment of circulating endothelial progenitor cells from baboon blood. Submitted & presented at TE 2002: International Workshop on Tissue Engineering. St. Gallen, Swetzerland. Feb. 24-27, 2002

6. Iuvone, P.Michael, Brown A.D., Haque, R., Weller, J., Zawilska, J.B., Chaurasia, S.S., Ma, MH.and Klein, D.C. Retinal melatonin production: Role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase. Investigative Ophthalmology & Visual Science 43(2): 564-572, 2002

7. Chen, Changyi, Surowiec, S.M., Morsy, A.H., Ma, MH. Intraperitoneal infusion of homocysteine increases intimal hyperplasia in balloon-injured rat carotid arteries.

Atherosclerosis 160, 103-114, 2002

8. Ma, Min-hui and Hanson, Stephen R. Outgrowth of circulating endothelial progenitor cells from baboon blood. Submitted & presented at the 6th Japan-China joint seminar on Histochemistry & Cytochemistry. Dec. 5-8, 2001

9. Grève, Pierre, Alouso-Gómez, A., Bernard M., Ma, MH, Haque, R., Klein, D.C. and Iuvone, P.M. Serotonin N-Acetyltransferase mRNA levels in photoreceptor-enriched chicken retinal cell culture: elevation by cyclic AMP. J. Neurochem.73(5): 1894-1900, 1999

10. Ma, Min-hui, Ji YS, Hu MR and Shen BF. Molecular cloning of E-selectin from human umbilical vein endothelial cells. J. Henan Medical University. 34(2): 45, 1999

11. Ma, Min-hui, Li JG, Zhang LH and Li RY. Study of the inhibition effects on influenza virus proliferation in chicken embryos by Yan Hou Jing. Henan Chinese Medicine. 18(10): 57, 1998

12. Li, Jianguo, Ma MH, Wang J and Ma MR. Comparative Atlas of Histology and Histopathology. Shandong Science and Technology, Jinan.1996

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