Research Scientist, antibody discovery & development, assay developmen
Resume:
Minha Park, Ph.D.
e-mail: *******@*******.***
SUMMARY
Demonstrated ability to develop and optimize novel research approaches and successful laboratory implementation of those ideas while collaborating with different groups. Experienced in optimizing workflow and generating scientific outcomes that have met milestone scientific achievements. Skilled in translating business objectives into action plans and scientific methods necessary to drive a team.
EDUCATION
Ph.D., Molecular Biology & Biochemistry University of California, Irvine, September 2001
M.S., Molecular Biology & Biochemistry University of California, Irvine, June 1997
B.S., Biological Sciences University of California, Los Angeles, June 1990
EXPERIENCE
Trellis Bioscience, Inc.
November 2005-August 2012
Scientist, Antibody Discovery
• Lead scientist responsible for the transition of company’s proprietary CellSpot technology from hybridoma to human B cell screening platform for discovery of completely human therapeutic antibodies.
• This work resulted in the first high affinity antibody, evaluated by industry partner for in-vitro and in vivo potency, and was successfully partnered for advancement to clinical trials for the treatment of respiratory syncytial virus.
• Subsequent projects which include antibodies for the neutralization of cytomegalovirus and influenza are currently being packaged for licensing.
• Responsible for overseeing all the human B cell screening while improving company platform for increased efficiency and providing leadership and supervision of team members towards successful outcomes of research.
Protein Design Labs, Inc.
May 2002-October 2005
Postdoctoral Research Scientist, Department of Protein Engineering
• Generation of various forms of antibodies for testing potency (dimeric IgG’s, dimeric IgA’s, afucosylated antibodies, humanized chicken antibodies, etc)
• Assay development for testing antibody potency for different effector functions such as ADCC (antibody dependent cellular cytotoxicity), CDC (complement dependent cytotoxicity), and apoptosis utilizing chromium release assay, LDH (lactate dehydrogenase) release assay, europium release assay, and flow cytometry.
• Other functions include antibody engineering, generation of antibody producing cell lines, protein engineering, protein expression and purification, and binding characterization.
Plexxikon Inc.
December 2001-April 2002
Special Consultant, Division of Protein Chemistry
Responsible for setting up the baculovirus expression system, generation of expression constructs for bacterial and baculovirus expression, expression and purification of various proteins.
University of California, Irvine
Jan 1997-November 2001
Graduate Student, Molecular Biology & Biochemistry
Professor: Andrea J. Tenner
Dissertation: “Characterization of Structural Parameters Critical for C1qRP”. Identification and functional characterization of the membrane receptor for C1q, the first component of the complement cascade. Demonstrated the role of functional C1qRP, the C1q receptor that enhances phagocytosis; characterization of C1qRP enhancement of phagocytosis by freshly purified human monocytes and macrophage cell lines; analysis of regulation of expression of C1qRP; characterization of glycosylation of C1qRP and the role of glycosylation in C1qRP cell surface stabilization; generated mutant constructs of C1qRP for bacterial and eukaryotic protein expression and purification.
University of California, Irvine September 1992-Jan 1997
Graduate Student, Molecular Biology & Biochemistry, Master’s
Professor: Gale A. Granger
Thesis: “The Role of TNF-Receptor-Releasing Enzyme and Soluble TNF Receptors in the Regulation of TNF Activity”. Developed an assay to detect and quantitate the activity of the TNF receptor releasing enzyme (TRRE), the metallo-protease involved in the generation of soluble TNF receptors, and characterization of its role in the regulation of TNF activity.
Veterans Hospital of West Los Angeles, CA
September 1990-1992
Research Assistant, Endocrinology Lab
Research project: Investigated the mechanism of action of TNF-a on thyroid cells in culture; characterized the effects of the thyroid stimulating hormone (TSH) and TNF-a on rat thyroid FRTL-5 cells by quantitation of various mRNA species and activation of NF-kB by EMSA.
PUBLICATIONS
•Collarini, E.J., Lee, FE., Foord, O., Park, M., Sperinde, G, Harriman, W.D., Carroll, SF, Ellsworth, S.L., Anderson, L.J., Tripp, R.A., Walsh, E.E., Keyt, B.A., and Kauvar, L.M. Potent High-Affinity Antibodies for Treatment and Prophylaxis of Respiratory Syncytial Virus Derived from B cells of Infected Patients. 2009. Journal of Immunology.
•Tsurushita, N., M. Park, K. Pakabunto, K. Ong, A. Avdalovic, H. Fu, A. Jia, M. Vásquez, and S. Kumar. 2004. Humanization of a Chicken anti-IL-12 Monoclonal Antibody. Journal of Immunological Methods, 295:9.
•Ward, M., C. Lin, D. C. Victoria, B. P. Fox, J. A. Fox, D. L. Wong, H. J. Meerman, J. P. Pucci, R. B. Fong, M. H. Heng, N. Tsurushita, C. Gieswein, M. Park, and H. Wang. 2004. Characterization of Humanized Antibodies Secreted by Aspergillus niger, Applied and Environmental Microbiology, 70:2567.
•Park, M. and A.J. Tenner. 2003. Cell Surface Expression of C1qRP/CD93 is Stabilized by O-Glycosylation, J. Cell. Phys. 196:512.
•Fonseca, M.I., P.M. Carpenter, M. Park, G. Palmarini, E.L. Nelson, and A.J. Tenner. 2001. C1qRP, a myeloid Cell Receptor in Blood, is predominantly expressed on Endothelial Cells in Human Tissue, J. Leukoc. Biol. 70:793.
•Kim, T.S., M. Park, R.R. Nepomuceno, G. Palmarini, S. Winokur, C.A. Cotman, U. Bengtsson, and A.J. Tenner. 2001. Characterization of the Murine Homolog of C1qRP: Identical Cellular Expression Pattern, Chromosomal Location and Functional Activity of the Human and Murine C1qRP. Mol. Immunol. 37: 377.
•Webster, S. D., M. Park, M.I. Fonseca, and A.J. Tenner. 2000. Structural and Functional Evidence for Microglial Expression of C1qRP, the C1q Receptor that Enhances Phagocytosis. J. Leukoc. Biol. 67(1):107.
•Nepomuceno, R.R., S. Ruiz, M. Park, and A.J. Tenner. 1999. C1qRP is a Heavily O-Glycosylated Cell Surface Protein Involved in the Regulation of Phagocytic Activity. J. Immunol. 162:3583.
•Katsura, K., M. Park, M. Gatanaga, E.C. Yu, K. Takishima, G.A. Granger, and T. Gatanaga. 1996. Identification of the Proteolytic Enzyme Which Cleaves Human p75 TNF Receptor in vitro. Biochem. Biophy. Res. Comm. 222:298.
•Burger, R.A., E.A. Grosen, G.R. Ioli, M.E. Van Eden, M. Park, M.L. Berman, A. Manetta, P.J. Disaia, G.A. Granger, and T. Gatanaga. 1995. Spontaneous release of interleukin-6 by primary cultures of lymphoid and tumor cell populations purified from human ovarian carcinoma. J Interferon Cytokine Res. 15:255.
•Pang, X.P., M. Park, and J.M. Hershman. 1992. Transforming Growth Factor-beta Blocks Protein Kinase-A-Mediated Iodide Transport and Protein Kinase-C-Mediated DNA Synthesis in FRTL-5 Rat Thyroid Cells. Endocrinology 131:45.
•Pang, X.P., N.S. Ross, M. Park, G.J. Juillard, T.M. Stanley, and J.M. Hershman. 1992. Tumor Necrosis Factor-Alpha Activates Nuclear Factor Kappa B and Induces Manganous Superoxide Dismutase and Phosphodiesterase mRNA in Human Papillary Thyroid Carcinoma Cells. J. Biol. Chem. 267:12826.
PATENTS
•Lawrence M. Kauvar, Stote Ellsworth, William Usinger, Krista Maureen McCutcheon, Ying-Ping Jiang, Fen Zhang, Bo Chen, Gizette Sperinde, Minha Park, Orit Foord, “High Affinity Human Antibodies to Human Cytomegalovirus (CMV) gB Protein,” submitted, patent application number 201********.
•Lawrence M. Kauvar, Stote Ellsworth, William Usinger, Krista M. McCutcheon, Minha Park, Bo Chen, Ying-Ping Jiang, “Antibodies Useful in Passive Influenza Immunization,” submitted, patent application number 201********.
SCIENTIFIC PRESENTATIONS
•Protein Engineering Summit 2012: Minha Park. “B Cell Display: Screening the Natural Human Antibody Repertoire for Broadly Neutralizing mAb Against Influenza A” Boston, MA, April 2012, Invited speaker.
•IBC Antibody Engineering and Therapeutics Meeting: Krista McCutcheon, Minha Park, Julia Gray, Natalie Chen, Keyi Liu, Evelene Lomongsod, Neal DeChene, Stote Ellsworth, Lawrence Kauvar, William Usinger, Scott Johnson, Dan Dlugolenski, Jon Gabbard, Ralph Tripp, Mark Tompkins . “Potent and Broadly Neutralizing Influenza A Human Monoclonal Antibodies Cloned from Memory B Cells in PBMC Culture” San Diego, CA, December 2011, Poster Presentation
•American Society for Virology Meeting: Minha Park, Keyi Liu, Neal DeChene, Michael McVoy, Stuart Adler, Stote Ellsworth, Lawrence Kauvar, William Usinger. “High Affinity Native Human Monoclonal Antibodies to CMV gB Glycoprotein” Minneapolis, Minnesota, July 2011, Poster Presentation.
•American Society for Virology Meeting: Minha Park, Stote L. Ellsworth, Keyi Liu, Krista M. McCutcheon, Neal DeChene, Evelene G. Lomongsod, Natalie Y. Chen, Julia Gray, Lawrence M. Kauvar, William R. Usinger. “Discovery of Broadly Cross Reactive Antibodies Against Influenza A Virus From Circulating Human B Cells” Minneapolis, Minnesota, July 2011, Poster Presentation.
•American Society for Virology Meeting: William R. Usinger, Scott K. Johnson, Shelly J. Samet, Dan A. Dlugolenski, Jon D. Gabbard, Minha Park, Krista McCutcheon, Stote L. Ellsworth, Lawrence M. Kauvar, Ralph A. Tripp, and Stephen M. Tompkins. “Human monoclonal antibody MAB53 shows effective cross-clade neutralization of influenza” Minneapolis, Minnesota, July 2011, Poster Presentation.
•American Society for Virology Meeting: Stephen M. Tompkins, Scott K. Johnson, Shelly J. Samet, Dan A. Dlugolenski, Jon D. Gabbard, Minha Park, Krista M. McCutcheon, Stote L. Ellsworth, Lawrence M. Kauvar, Ralph A. Tripp, and William R. Usinger. “Novel cross-subtype, fully human, influenza hemagglutinin-specific monoclonal antibodies ” Minneapolis, Minnesota, July 2011, Poster Presentation.
•Sixth International RSV Symposium: E. Collarini, F. E.-H. Lee, O. Foord, M. Park, B. Harriman, D. Chen, G. Sperinde, N. DeChene, J. Zhang, L. Jones, I. Sanz, R. Tripp, L. Anderson, E. Walsh, S. Ellsworth, B. Keyt, L. Kauvar. “Analysis of the Human B Cell Repertoire Against Respiratory Syncytial Virus G Protein” Marco Island, Florida, October 2007, Poster Presentation.
•Complement Associated Diseases, Animal Models and Therapeutics Workshop: A.J. Tenner, S. Ruiz, and M. Park, “Characterization of Parameters Influencing the Enhancement of Phagocytosis by C1qRP,” Santorini, Greece, October 2001, poster and oral presentation by A. Tenner.
•Complement Workshop: M. Park and A.J. Tenner, “Glycosylation Stabilizes the Surface Expression of C1qRP,” Snowbird, UT, July 2000, Poster Presentation.
•Midwinter Conference of Immunologists: M. Park, S.D. Webster, and A.J. Tenner, “C1q Interacts with C1qRP in Phagocytic Cells to Enhance FcR- and CR1-mediated Phagocytosis,” Asilomar, CA, January 2000, Poster Presentation.
•FASEB Meeting: M. Park, T.S. Kim, and A.J. Tenner, “The Mouse Homologue of the Human C1qRP Enhances Phagocytosis in Murine Macrophage Cell Lines,” Washington, D.C., April 1999, Oral and Poster Presentation.
•Complement Workshop: R.R. Nepomuceno, S. Ruiz, M. Park, and A.J. Tenner, “Crosslinking of C1qRP, a Heavily Glycosylated Transmembrane Protein, by Monoclonal Antibody, R3, Enhances Phagocytosis in the Absence of its Endogenous Ligands C1q, MBL, or SPA,” Mainz, Germany, October 1997, Poster Presentation.
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