Dr. H Jerome Coyne III

**** ********* **. **

Southport, North Carolina 28461

email: u4b9pz@r.postjobfree.com

phone: 910-***-****

Objective: Perform biological research that will utilize my technical skills and further my scientific and professional development.

Professional Experience:

08/2011 – 08/2012 Post Doctoral Fellow, Biochemistry, Indiana University

Structural characterization of prokaryotic, transcriptional regulators involved in copper sensing.

Solution state NMR spectroscopy utilized to assign protein backbone of structural candidates.

Acquire and interpret data from ESI-LC/MS, MALDI-TOF, AUC, AA, and gel filtration.

Serve as an in lab technical expert to assist graduate student in their research projects.

Research new technologies and participate in the peer review process.

05/2007 – 07/2011 Post Doctoral Fellow, Biochemistry, University of British Columbia

Structural characterization of transcription factor proteins implicated in the development of cancer.

Dynamic and structural studies accomplished utilizing 2D and 3D NMR spectroscopy.

Project involvement included gene cloning, protein purification, data acquisition, and data analysis.

Structure calculations performed utilizing ARIA and CYANA programs.

Technical expertise in liquid chromatography: Ni+2 affinity, ion exchange, and gel filtration.

Proficient with recombinant DNA techniques: insert/overlap PCR, mutagenesis and cloning.

Routine use of instrumentation such as: MALDI-TOF, Circular Dichroism, HPLC and SDS-PAGE.

01/2001 – 06/2001 Teaching Assistant, Chemistry, University of Iowa

Prepared and directed problem solving discussion groups for premed chemistry students.

06/1994 – 12/1997 Research Scientist, Reckitt & Colman

Created analytical methods, researched new technology, and performed competitor analysis.

Introduced new methods to manufacturing sites and served as a technical analysis representative.

Accumulated operating and theoretical knowledge in HPLC, GC, GC/MS, FTIR, IC & AA.

01/1993 – 05/1994 Quality Control Chemist, Finished Products, Schein Pharmaceutical

Education:

06/2001 – 04/2007 University of Utah, PhD Biochemistry.

Research focused on the structural and functional characterization of metalloproteins involved in cytochrome c oxidase assembly.

Developed and optimized recombinant expression systems for metalloproteins.

Characterization achieved through spectroscopic techniques such as NMR, UV-VIS, & AA.

Functional significance of proteins assayed by monitoring respiration both in vitro and in vivo.

Plasmid construction, mutagenesis, Western Blot, mitochondrial fractionation, yeast biology

Significant portion of thesis data collected while at the University of Firenze, Italy.

Additional PhD Accomplishments

Teaching Assistant for Structural Methods Course with emphasis on MS, NMR, & X-ray.

Developed critical thinking on current literature through departmental and group Journal Clubs.

Involvement with a diverse research group supplemented my knowledge of molecular biology.

01/1998 – 12/2000 University of Iowa, Masters in Chemistry.

Studies were conducted on the activity and mechanism of lactoperoxidase.

Paramagnetic NMR and EPR were utilized to study potential mechanisms and intermediates.

HPLC and Column Chromatography routinely used in sample preparation.

09/1988 – 12/1992 University of Delaware, B.S. Chemistry, Minor Economics

Grants & Awards:

University of Utah Hematology Training Grant 2002 – 2003

University of Utah Biological Training Grant 2003 – 2004

University of Firenze Fellowship 2005 – 2006

Publications:

Coyne HJ 3rd, Giedroc DP Backbone resonance assignments of the homotetrameric (48 kDa) copper sensor CsoR from Geobacillus thermodenitrificans in the apo- and Cu(I)-bound states: Insights into copper-mediated allostery. BIOMOL NMR ASSIGN 2012 (BNMR-D-12-00034R1 accepted as of 09/14/12)

Coyne HJ 3rd, Soumya D, Okon M, Green SM, Bhachech N, Graves BJ, McIntosh LP. Auto-inhibition of ETV6 (TEL) DNA-binding: appended helices sterically block the ETS domain. J. Mol. Biol. 2012: 421,1, 67-84.

Green SM, Coyne HJ 3rd, McIntosh LP, Graves BJ. DNA binding by the ETS protein tel (ETV6) is regulated by autoinhibition and self-association. J Biol Chem. 2010; 285, 24, 18496-504.

Coyne HJ 3rd, Ciofi-Baffoni S, Banci L, Bertini I, Zhang L, George GN, Winge DR. The characterization and role of zinc binding in yeast Cox4. J Biol Chem. 2007; 282,12, 8296-8934.

Structures: available at www.rcsb.org

2LF7.pdb

2LF8.pdb

20DX.pdb

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