Technician Engineer
Resume:
FRANK (FEIYUE) LI
(US Citizen)
Germantown, MD 20874
Phone: 301-***-****
Email: ********@*****.***
Summary
Over 20 years of extensive experience in biochemistry and chemistry techniques. Have lead teams of technicians and works well with teammates. A self-motivated, hard working, master new skills quickly, and have good communication skills person. Have computer skills in database and Microsoft Word, Excel and corporate intranets.
Skills and Expertise
Expert in biochemistry and chemistry techniques, experiences at protein/DNA,
RNA, antibody analysis as well as QC
Strong skills with analysis methods ( HPLC, MS, CE, ELISA, GC/FID, AAS, AFS etc) especially in the chromatography field
Extensive working with DNA/RNA, protein, manufacturing, synthesis, and purification
Experience in manufacturing processing in GMP/GLP environment ensured production timing and quality
Experience in managing production flow with excellent problem shooting skills
Technical documentation / presentation
writing and update procedures
3 years of supervising and mentoring experience
Professional Practice
• Protein, antibody extraction, purification, and analysis by IEX, HIC, SEC column, with AKTA Purifier 10, GE Cross-Flow Filtration, Agilent Affinity LC as well as QC operation with MS, CE
• DNA/RNA products assay using HPLC Agilent 1100, Waters HPLC, PAGE,COP Cartridge,, UV/VIS spectroscopy
• Automated PE/ABI 377 sequencer for human DNA sequences detection
• Western Blot, Genotyping, PCR, Cell culture and bacterial fermentation
Experience
Life Technologies (formerly Invitrogen) Frederick, MD
Sr. Manufacturing Technician Mar ’02 – Dec ‘11
• Worked in the molecular biology lab under the GMP environment to perform QC sample analysis with MS (MALDI-TOF, Triple Quads) and CE (Combisep).
• Responsible in the team to purify RNA, DNA/protein including large and small scale by using HPLC Waters 2695, Agilent LC, PAGE, ELISA and COP Cartridge methods. As the main force of RNA purification, ensured the all RNA purification timing and quality. DNA/RNA 96-well plate based quantity assay. Distinguished the results of mass spectrometry and CE. Dealt with all paper work and database to ensure the on-time delivery of products. Especially kept high passed rate for the team quarterly goal.
• As a magnetic protein, purified Dynabeads products with SEC column, and pre-coupled it with a biomolecule such as an antibody, protein or antigen, DNA/RNA probe etc.
• Responsible in the team to synthesize DNA and RNA primers. Operated Jurassic Synthesizer, Oligo Pilot, ABI 3900 and Mer made12 to produce various DNA, RNA sequences. Some of them were called semiconductor Sequencing that would be need more carefully. Modified or labeled oligos with special molecules and dyes (Phosphate, Biotin, HEX, TET, Alexa488 etc.).
• Standardized and reviewed laboratory documentation to help the company that gained ISO 9001:2008 certifications.
• Improved on the procedure of RNA purification. As a result, the RNA purification cost was reduced 30% and accomplished in 2 hours less time.
• GMP production documentation, including rewrote and devised SOPs & batch records.
• Trained the new group of people to help the company when it relocated production to CA.
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FMC Corporation Baltimore, MD
QC Lab Technician Nov ’00 – Mar ‘02
• Performed HPLC, Orbitrap LC/MS, and AAS to determine intermediates of insecticides and final products at the Quality Control Lab. Focused on resolutely implemented SOP to ensure high-quality results to be achieved rapidly.
• Environmental monitoring that included water and soil sample testing.
• Calculated analytical results and transferred to data base.
• Maintained and prepared the analytical equipments weekly.
Georgetown University / Navy Medical Research Center – Biomedical Research Rockville, MD
Lab Technician Apr ’00 – Oct ‘00
• Performed SEC column and Affinity HPLC for monoclonal antibody analysis from human serum, including AFM1 identification.
• Responsible genotyping for HLA-DR, extract DNA from human blood cells.
• Performed full length genes PCR-Taqman, genotyping and PAGE, autoradiography. Hybridized dot blots with nonradioactive detected labeled (antibody-Lumiphos complex) probes and ELISA for antibody assay.
• Experienced in using automated PE/ABI 377 sequencer for marrow DNA sequencing analysis and registry.
Food Research Institute /Central Lab Tianjin, China
Laboratory Engineer/ supervisor Apr ’84 – May ‘98
• Gained proficiency in using various analysis machines such as HPLC, UV-visible Absorption, Atomic Absorption, Atomic Fluorescence Spectroscopy, IR, GC/MS, Scanning Electron Microscopy.
• Developed the new type of beer yeast with suspension cell culture and bacterial fermentation methods.
• Extraction and analyzing protein with Affinity/LC, PAGE.
• Environmental monitoring that included water and soil sample testing.
• 3 years of supervising and food chemistry analysis mentoring experience.
• Written laboratory SOPs and working flow procedure to gain ISO9000 international certification.
• Devised valid graphite oven to improve absorbance stability in AAS machine.
• New type of application of science in food industry and commerce technique developing.
Education
B.S. degree in Analytical Chemistry, Tianjin University, Tianjin, China (1983)
Notable Projects
• Participated in the setting of China’s National Standard for the determination of zinc contents in food using AFS analysis.
• Improved on the protocol of RNA purification to reduce production cost.
Honors and Awards
• Recognition for dedication in training new employees 2011
• Excellence Award for zero error ratio 2005-2007
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