Suraj Saraswat

**** ******** **., *** # ***, Toledo, OH 43606

Phone: 419-***-****, Email: txvjcd@r.postjobfree.com

SUMMARY Developed methodology for absolute quantification of biomolecules and determined in vivo concentrations using expertise in handling highly sophisticated instruments (Liquid Chromatography (LC), Mass Spectroscopy (MS), Microscopy). Developed methodology for early cancer detection using glycan analysis. Imaged peptides and proteins in brain and spinal cord tissue sections by applying imaging mass spectroscopy approach. Determined efficiency of energy transfer from fluorescent proteins to nanoparticles (FTIR, Fluorescence).

EDUCATION

Ph.D in Chemistry, University of Toledo, 3.6/4.0, (May 2012)

Master of Arts in Biochemistry, University of Scranton, 3.6/4.0, (Aug 2008)

(Thesis: Antioxidant effect of polyphenols and their binding studies with LDL/VLDL)

Master of Science in Chemistry, University of Delhi, India (May-2004)

Bachelor of Science in Analytical Chemistry, University of Delhi, India (May 2001)

EXPERTISE • INSTRUMENTATION

• Quadrupole-Time of flight (Q-TOF) Mass Spectrometer (MS): Waters Synapt G1 HDMS-Set up and operation (Dec 2008-May 2011)

• Ion Trap and MALDI-TOF-TOF Mass Spectrometer: Bruker Daltronics ultrafleXtreme (Dec 2008-May 2011)

• Electrospray Ionization (ESI) MS: Bruker Daltonics Esquire-LC and Waters Synapt G1 HDMS (Dec 2008-May 2011)

• High Performance Liquid Chromatography (HPLC): Shimadzu Prominence Series (Dec 2006-May 2011)

• HPLC-MS (Dec 2006-May 2011)

• IMMUNOAFFINITY TECHNIQUES

• Immunoprecipitation, Immunoaffinity Chromatography, Gel Permeation chromatography, Solid Phase Extraction, Protein Precipitation, Microwave-Assisted Proteolysis.

• BIOINFORMATICS

• Proteome Discover, Mascot, MS-Product, MS-digest, Bio-Tools.

• BIOTECHNOLOGY

• SDS-PAGE, Western Blotting, Cell Culture, Imaging, ELISA

• Experienced with GC-MS, LC-MS, Light Microcopy, Electron Microscopy, FTIR, Fluorescence Spectrophotometer and Ultraviolet and visible (UV-Vis) spectroscopy instrumentation.

• Extensive experience with protein purification and characterization methods, with laboratory safety, with performing chemical analysis and developing and validating analytical methods.

COMPUTER SKILLS

SOFTWARE

PACKAGES

IBM-PC, Microsoft-PC, Apple-PC, Windows 2007, MS Office 2007 (Word, Excel, PowerPoint), Origin, Internet, World Wide Web, email, Netscape or MS Internet Explorer, Scifinder.

Chem Draw (2D, 3D), Scion Image for SEM, Software’s related to analytical instrument operation, Masslynx [for analysis through MS], LC Solution [for analysis through HPLC], NIS-Elements BR 3.0 [for analysis through Light Microscopy].

AWARDS AND FELLOWSHIPS

• Graduate Assistantship Aug 2008 to present, University of Toledo, Toledo, OH.

• Graduate Assistantship Aug 2006 to Aug 2008, University of Scranton, Scranton, PA.

• Received College Crust awards during Graduation.

• Outstanding graduate student award, University of Scranton, Scranton, PA.

WORK HISTORY

ACTIVITES Graduate and Research Assistant, University of Toledo, (Aug 2008- present)

• Designed and developed new strategy and methodologies for absolute quantification of peptides and proteins in real biological samples.

• Develop and validate chromatographic procedures for small to big chemical entities.

• Developed strategy for energy transfer from fluorescent proteins to Nanoparticles.

• Developed methodologies for cancer diagnosis by detecting glycans over expressed in cell surface glycoprotein.

• Modified protocol for imaging of peptides and proteins present in organs via tissue sectioning using imaging mass spectrometry (IMS).

• Led team of undergraduates and high school students to image proteins in sub cellular environment after labeling with dyes using light microscopy.

• Research and development of currently available cell culture and peptide purification protocols.

• Development and use of HPLC and gel electrophoresis based methodologies for sample separation, purification and characterization.

• Prepare samples, acquire MS data and analyze proteins/peptides identification, characterization, and quantification results that demonstrate the uses and benefits of novel protein MS workflows and reagents.

Graduate and Research Assistant, University of Scranton, (Aug 2006- May 2008)

• Determined in vivo concentration of vitamins using liquid chromatography assay

• Determined effect of polyphenols on the oxidation of Low density lipoproteins (LDL) and very low density lipoproteins (VLDL) and also their binding affinities.

• Operate, maintain, and troubleshoot analytical instruments and chemistry processes

• Follow Chemical Hygiene Plan and other safety procedures

Chemist, Shriram Institute for Industrial Research, (January 2004 – July 2006)

• Isolated and purified analytes for detection of sample purities.

• Physical and chemical analysis of metal samples for government certified standards.

• Interpret test results, write reports, and effectively communicate with divisional head and customers.

• In-depth working knowledge of regulatory compliance requirements (GLP/GMP)

• Knowledge of and experience in materials handling (bio and chemical hazardous materials and controlled substances)

• Exhibited Poster in ASMS conference on quantification of peptides and proteins using native fluorescence (May 2011)

• Oral presentation on quantification using native fluorescence of peptides and proteins (March 2011)

• Exhibited Poster in Pittcon on energy transfer from proteins to nanoparticles (March 2010)

• Exhibited Poster in ASMS conference on effect of labeling on ionization efficiency of peptides and proteins (May 2010)

• Participated in Cancer Awareness program in University of Scranton

• Member, University of Toledo, International Club (August 2008- present)

• Member, University of Scranton, International Club (August 2006- August 2008)

• Organized symposium for science students

• Participated in cultural programs conducted by University of Scranton and University of Toledo

Publications • Dragan Isailovic, Yang Xu, Tyler Copus, Suraj Saraswat, and Surya M. Nauli, "Multimodal Spectral Imaging of Cells Using a Transmission Diffraction Grating on a Light Microscope," Appl. Spectrosc. 65, 575-583 (2011)

• Saraswat, Suraj; Desireddy, Anil; Zheng, Desheng; Guo, Lijun; Lu, H. Peter; Bigioni, Terry; Isailovic, Dragan, "Energy Transfer from Fluorescent Proteins to Metal Nanoparticles" J. Phys. Chem. C, 2011, 115 (35), 17587- 17593.

• Nidhi Jaiswal, Suraj Saraswat, Manohar Ratnam, Dragan Isailovic, “Analysis of Folate Binding Protein and Associated N-Glycans by Mass Spectrometry”, J. Proteome Res. Accepted under review.

Profile http://www.linkedin.com/pub/suraj-saraswat/9/586/51

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