Quality Assurance Project

RAMANJANEYA REDDY. Y

Plot No: ***, *th Cross, R.K.Lake View,

Hebbagodi, Bangalore - 500100, Karnataka, India

Email: *******@*****.*** w Mob: +91-968*******

Enthusiastic & diligent Senior Research Associate with expertise in High throughput Biochemical and Cellular assay development with insightful experience in the field of Drug Discovery & Development,

Profile at a Glance:

Æ High throughput cellular and biochemical assay development to screen NCE’s for pre clinical drug discovery in collaborative projects.

Æ Expertise in Enzyme assays, Cell based assays, radio nucleotides, Estimation of cytokines, ELISA, SPA assay, determination of cAMP, cytotoxicity and cell viability assays, Graph Pad Prism software.

Æ Strong abilities in managing operations with key focus on bottom line profitability by ensuring optimal utilization of resources.

Æ A motivated and diligent team leader utilizing excellent communication, interpersonal skills with strong analytical mind-set.

Æ Currently associated with Syngene International Ltd, Bangalore as Senior Research Associate II

PROFESSIONAL EXPERIENCE

Syngene International Ltd., (BIOCON Company) Bangalore, India

Senior Research Associate II (Jul 2009 – Till date)

Key Responsibilities:

Validation and Standardisation of the assays, and screening of compounds in collaborative projects.

Screening of the compounds to identify PARP-1 inhibitors that selectively kill BRCA -/- cells and also with purified PARP enzymes in SPA assay using 3H NAD radioligand.

Established a panel of off target assays such as Tankyrase-1, vPARP, PARP-2 in cancer project.

Developed cAMP functional assay for overactive bladder in human beta3 adrenoreceptor using TR-FRET technology.

Successfully established a panel of off target assays to determine the cAMP in Human beta1 and Human beta2 adrenoreceptor using TR-FRET technology.

Analysis of Data, Uploading the data at the Client site, communicating with the client and presenting the same on a weekly basis.

Key Accomplishments

Lead and trained junior level in cell based and biochemical assay.

Coordinated with Chemistry team with data related issues.

Involved in preparation of study reports.

Strong ability to effectively plan and organize work activities and prioritise task completion to meet deadlines.

Screened around 600 compounds to identify PARP-1 inhibitors.

Played a major role in the successfully development of a PCC candidate for the cancer project in a slim time line. Currently this back up molecule is headed for safety evaluation.

GVK BIOSCIENCE Ltd. Hyderabad, India

Research Associate (Dec 2005 – Jun 2009)

Key Responsibilities:

High throughput cellular and biochemical assay development to screen NCE’s for pre clinical drug discovery

Developed biochemical assays and screened the compounds for various targets in proteases such as MMP’s, Caspases, Cathepsins, ACE, and TACE using FRET Peptide substrates.

Developed kinase assays such as JNK, P38 alpha MAPK, Cdk1, ERK2 using Radioactive Gamma P32ATP.

Developed assays for cytotoxicity and cell viability (MT, Alamar blue) using regular cell culture format (96 and 384 well formats)

Quantitative determination of Nitric oxide Concentrations using RAW 246.7 cell line

Developed alpha Glucosidase assay using BHK cell line

Estimation of TNF-alpha, IL6 and IL10 from Human Blood Cells (PBMC)

Estimation of IL10 from RAW 246.7 cell line

Determination of caspase-3 (Apoptosis assay) from HeLa and HepG2 cell lines

Developed Platelet aggregation assay from Human blood cells

ELISA (Sandwich and bridging to capture antigen/antibody)

Basic Molecular techniques like preparations of competent cells, Plasmid Isolation (Mini,Midi) Transformation of Plasmid DNA, Cloning, Protein expression and optimisation.

Ensured that all work is in compliance with all pertinent SOP, and Safe Work Practices

Key Accomplishments:

Successfully screened nearly 800 compounds for Nitric oxide and Glucosidase assays.

Successfully screened nearly 50 compounds for the estimation of cytokines.

Efficiently initiated project meeting before starting any Project and actively participated in coordination of the projects.

Efficiently assisted in the development & revision of laboratory policies & Standard Operating Procedures.

Sven Gene Tech Ltd. (Jupiter Bioscience Ltd) Hyderabad, India

Senior Biochemist (Sep 2003- Nov 2005)

VIMTA Labs Ltd. Hyderabad, India

Biochemist (Jun 2002 – Aug 2003)

PROFESSIONAL DEVELOPMENTS

Trainings, Seminars, Workshops & Awards:

Attended two days seminar for Quality Assurance in GVK BIOSCIENCE.

Good Lab Practice (GLP) certificate: Internal Auditor training on GLP: A hands on experience, held in Ahmedabad. Conducted by Confederation of Indian industry (CII) Institute of Quality, Bangalore.

Advanced Diploma course in computer programming. CDAC, GIST (A Scientific society of Department of electronics, Govt. of India.

Received GVK BIO Value star awarded in performing outstanding contribution specially customer focus, commit to excel, respect, and Integrity.

Received Reward and Recognisation (R&R) award in GVKBIO.

Received the best scientific team in the BIOCON science achievement awards 2009-2010.

Technical Expertise

Liquid Scintillation counters (Top count NXT, Perkin Elmer)

Multidetector Micro plate reader (POLAR star optima, BMG Labtech)

Envision Multidetector reader (Perkin Elmer)

Spectramax (Molecular device)

Tecan Freedom evo150 –Liquid Handling system

Graph pad prism software for calculations in High throughput assays

Research Publications

Biochemical and PCR- RAPD characterization of Pseudomonas fluorescens produced antifungal compounds inhibit the rice fungal pathogens invitro, Prasanna Kumar Reddy.B, Ramanjaneya Reddy.Y, and M.S.Reddy. Journal of Pure and Applied Microbiology; volume 3 No.1 page No.347-350 (2009).

Determination of Pantoprazole sodium and Lansoprazole in individual dosage form tablets by RP-HPLC using single mobile phase, Prasanna Kumar Reddy.B, Ramanjaneya Reddy.Y and Ramanchandran. D, E-Journal of Chemistry, Volume-6, issue2, page No.489-494 (2009)

EDUCATIONAL QUALIFICATIONS

M.Phil (Biochemistry), Acharya Nagarjuna University, A Grade (2005-2006)

M.Sc (Biochemistry), Bharatidasan University, 79.5% (1999-2001)

B.Sc (Botany, Zoology, Chemistry), Sri Venkateswara University, 72%(1996-1999)

PERSONAL DETAILS

Date of Birth: 4 June 1977

Languages Known: English, Hindi and Telugu

Permanent Address: S/O.Y.Malla Reddy, Near Ramalayam, Chilamakur, Yerraguntla (Md), Kadapah (Dt), Andhra Pradesh, India-516310

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