Experienced molecular biologist/neuroscientist

Richard S. Stewart, Ph. D.

**** ******** ***. *** ** St. Louis, MO 63108 

Ph:314-***-**** E-mail:***********@*****.***

SUMMARY OF QUALIFICATIONS:

• Ph. D. biologist with over 10 years expertise in biochemistry, cell biology, molecular biology, and neuroscience

• Productive researcher, fast learner, versatile, good organizational skills

WORK EXPERIENCE:

Instructor 2005-2008

Postdoctoral fellow 1998-2005

Dept. of Cell Biology and Physiology

Washington University School of Medicine (St. Louis, MO).

• Initiated and advanced a research project investigating the role of alternate topologies of the prion protein

• Utilized multiple experimental systems to advance the project, beginning with in vitro translation assays, to cell culture models, to a transgenic mouse model for a neurodegenerative illness

• Developed expertise in cell biology and neuroscience techniques, including cell culture and transfection, primary neuron culture, apoptosis, histopathology, confocal microscopy, and microarray analysis

• Supervised graduate students and undergraduates on research projects

• Strong record of publication in high-quality journals

• Advisor: David Harris, M.D. Ph. D.

Research Associate, Dept. of Endocrinology 1989-1992

Northwestern University School of Medicine (Chicago, IL)

• Conducted experiments and prepared all reagents in a small laboratory

• Developed expertise in developmental biology and molecular biology techniques

• Trained colleagues in molecular biology methods

EDUCATION:

Ph.D. in Cellular and Molecular Biology 1992-1998

University of Wisconsin (Madison, WI)

• Studied the enzymology of mRNA degradation in mammalian cells

• Gained extensive experience in protein purification, RNA manipulation, cell culture, and virology

• Thesis Advisor: Jeff Ross, M.D.

B. A. in Biochemistry 1985-1989

Northwestern University (Evanston, IL)

• National Merit Scholar

• Earned certificate in History and Philosophy of Science

• Earned two varsity letters as a student trainer

HONORS AND AWARDS

• Ruth L. Kirschstein National Research Service Award, NIH, 2001-2003.

• Department of Defense National Prion Research Program (NPRP), Career Transition Award, 2003-2008

PROFESSIONAL SOCIETIES

• American Society for Cell Biology 1998

• Society for Neuroscience 2004-2008

SPECIFIC EXPERTISE:

• Molecular biology/cloning

• Cell-free translation/microsome isolation

• RNA purification and analysis

• Protein purification (HPLC)

• Large-scale (10L) cell growth

• Cell culture/stable cell line creation

• Immunocytochemistry/Confocal microscopy

• ELISA/Monoclonal Antibody screening

• Transgenic mouse characterization

• Neurodegenerative disease phenotyping

• Histopathology

• Primary neuronal cell culture

• Neuronal cell death assays

• Assay development/high-throughput screening

• Microarray sample prep and bioinformatics analysis

• Virology (Herpesvirus and Lentivirus)

REFERENCES: Available upon request.

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