Molecular Genetics, Cell Culture(mammalian and plants), fluorescent im

Jayita Goswami Citizen of United States

**** *. ********** **, *****, ID, 83706, Email: **********@*****.***,

PH-208-***-****

SUMMARY

WORK EXPERIENCE & EMPLOYMENT Highly motivated, quick learner with a distinguished hands on knowledge base encompassing a broad spectrum of Biological research, with special expertise in Cell biology, Molecular biology, Biochemistry, Plant biology and Forensics, committed to excellence, smart and detail oriented by nature. Goal to be a senior scientist in a reputed organization.

Boise State University, Boise, ID, Research Associate, 2007-2011

Cancer drug discovery project: Drug discovery purposes worked with 199 small rare peptides and tested them against several cancer and normal human cell lines; explored cell proliferation assay, cytotoxicity assay, ELISA, apoptosis assay, mitochondrial health assay, super oxide detection assay, confocal microscopy and fluorescent microscopy with broad range of mammalian cell lines, observed peptides activity at subcellular level, design and execute experiments independently.

Forensic Project: Designed a DNA barcode with rare DNA sequences which will help to identify accidental forensic DNA mixture related problems; and validated it with forensic DNA typing (STR/PCR) kits, mitochondrial DNA sequencing kit (ABI and Promega); proficient with ABI’s 310, 3130 genetic analyzer/sequencing instruments and software’s; in a side project extracted DNA from histology slides (self-made protocol) for downstream molecular biology experiments (2010).

NIH/NIDDK, Arizona, Technical Research Fellow, 2004; worked as a NIH technical research fellow at Phoenix Indian Medical Center and investigated Type II Diabetes disease in human; explored several human genes for mutation; experiments were PCR, Taqman and Sequencing based.

Arizona State University, Tempe, 2000-2003; graduate research on photosystem one protein complex of Chlamydomons reinhardtii species; made several mutants with mutagenic PCR technique. The mutagenic plasmids were transfected into Chlamydomonas chloroplast by gene gun (particle bombardment) method; selected mutants were used to study electron transport of photosystem one protein complex; this protein complex’s stability was measured by SDS-PAGE and LDS-PAGE; worked as a teaching assistant and research assistant at Arizona State University.

EDUCATION Masters (I) from Arizona State University, US (12/18/2003), study was interdisciplinary, main focus was on molecular biology.

Relevant subjects studied- Photo-biology, Biochemistry, Plant molecular biology.

Masters (II) in Cytogenetics/Tissue Culture/plant breeding from Visva Bharati University, India (11 Jan/1998).

Relevant subjects studied- Biochemistry, Botany, Immunology and Biophysics.

Bachelors in Botany (major), Zoology (minor) and Chemistry (minor) from Calcutta University, India (25th Feb/1993).

MOLECULAR / CELLULAR / PLANTS/ BACTERIA’S

DNA extracted from different sources such as bacteria’s, human buccal swabs, agricultural plants, rabbit tissues, and cow tissues for genotyping, DNA sequencing, STR/PCR and cloning.

Cloned and followed the expression of algal photosystem I gene and bacterial pTrc-tolC gene on antibiotic containing culture plates.

Proficient with qPCR, Mutagenic PCR, Primer design, Point mutation, Ligation and Agarose Gel electrophoresis; DNA Transfection through Gen gun, Heat shock and Electroporation; DNA hybridization by Southern blot.

Protein quantitation by Shimadzu spectrophotometer, Western blot of membrane proteins, Protein extraction by Affinity chromatography.

Proficient with mammalian cell culture (adherent and suspension), Bacteria culture and bacterial protein extraction, Algae culture, Plant tissue culture (organ and suspension); Bacterial media preparation, Fungal and Algal media preparation, Staining, Reagent preparation.

INSTRUMENTATION

Synergy plate reader, 310/3130 genetic analyzer, PCR instruments, Eppendorf realplex, Bio-Rad Gel imager, Gel electrophoresis, Nanodrop, Centrifuge, French press, Nebulizer, Spectrophotometer, Bacterial growth chamber, Fluorescence inverted scope, Confocal microscope.

COMPUTER SKILLS/MANAGEMENT SKILLS

Proficient with Microsoft word, Excel, Spreadsheet, Nanodrop, Bio-Rad Gel imager, Oligo/primer design, DNA sequencing software’s and Genemapper ID-X (made by ABI) ; used BLAST and SNP detection (SeqScape or Sequencing Analysis) software made by ABI.

I use PowerPoint, Scanner, Adobe PhotoShop, Paint, Photo Editor; Spot advanced imaging software, GIMP photo editor, GraphPad Prism, Zen 510 etc. for presentation.

Search scientific journals on regular basis to design better experiments and help colleagues to set up their experiments; write and present scientific reports periodically, manage order/inventory in a laboratory

ACTIVITIES

88th annual meeting of the AAAS Pacific Division, oral presentation, “Bioactivity of herbal medicine”, 2007, Boise.

Attended International symposium of Human Identification, 2009, Las Vegas.

24th annual symposium of protein society, poster presentation, “Small peptide molecules effect on cancer cells”, 2010, San Diego.

Workshop attended- (a) DNA mixture interpretation, AAAs meeting (8 hrs); (b) Validation of new techniques and assays in the human identification laboratory, Promega meeting.

PUBLICATIONS

Goswami, J. et al, “Detection of Forensic DNA contamination with nullomer barcodes”, this paper is going through submission procedure.

Cancer research paper is getting ready for submission.

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