Quality Control Assistant

MARY ANITHA GUDIPATI

**** *** ******, *******-** -79415. Email: *.**********@*****.*** Phone: 806-***-****.

OBJECTIVE

To seek a challenging position in a reputed healthcare industry which is highly innovative and focused on high complexity testing where my knowledge, skills and experience may contribute to the core values of the organization.

SUMMARY

• Three years of experience in Molecular Genetics, FISH, Urovysion and array-based CGH micro array.

• Masters in Biology and Biochemistry with three years of research experience.

• Two years of teaching experience.

• Excellent writing and presentation skills.

• Diligent, thorough and highly adaptable. Capable of contribution as an individual contributor as well as working on a team.

SKILLS

• Biochemistry: SDS-PAGE, Western Blotting Technique, Enzyme Linked Immuno Sorbent Assay, Immunohistochemistry, HLA typing of blood.

• Cell Biology: Preparation of culture media and Routine animal cell culture, Pre-treatment of amniotic fluids.

• Molecular Biology: Profound experience in genomic DNA isolation from whole blood, PCR, restriction digestion, gel preparation, Gel electrophoresis, Enzyme labeling, Measuring the purity of DNA and labeling efficiency.

• Microbiology: Preparation of bacteriological media, Routine sterilization.

• Analytical Instrumentation: Imaging system for visualizing DNA, use of micro plate reader, Use of fluorescent microscope.

Computational skills:

• Use of programs like Blast and clustalW in Bioinformatics

• Proficient in Microsoft Excel, Word, PowerPoint, Adobe Photoshop.

Database and analysis skills:

• Familiarity with Data modeling, Relational databases, Oracle SQL, and Statistical analysis using SPSS.

WORK EXPERIENCE

Cytogenetic Technologist, Texas Tech University Health Science Center, Lubbock, Texas, United States (July 2009 – Present).

a) Test development: Methodology development, test validations. Interpretation, diagnosis, troubleshooting, problem-solving and quality control.

b) Case review: Maintaining quality and accuracy requirements, resulting, pre-review, preparation of all patients’ reports before medical geneticist sign-out.

c) Professional experience: Prenatal diagnosis by FISH, cell and tissue culture techniques, wet lab and dry lab techniques and chromosome identification by following ISCN 2009 Nomenclature.

d) Clinical experience:

Peripheral blood and bone marrow culture and harvest, analysis, diagnosis and interpretation of micro-deletions, constitutional blood, bone marrow, and hematological malignancies related to molecular and FISH and Paraffin embedded FISH testing using Applied Imaging Cytovision.

Molecular genetics includes working on ALL (adult and child), CLL, CML, MDS, MM, AML panels and COG cases. Individual probes include working on enumeration probes, deletion probes, break-apart probes, translocation dual-fusion probes, inversion probes and fusion-deletion probes and works with CAP challenges every year.

FISH:

Preparation of Samples: Prepares samples for hematological FISH by creating smears, and/or preparing slides from harvested cultures. Prepares samples for Urovysion.

Washing and Handling of FISH slides: Has knowledge of and follows the appropriate protocols for the washing and handling of FISH slides.

Microscopic Analysis: Analyzes interphase nuclei for hematological abnormalities.

Imaging: Uses Applied Imaging Cytovision or other capturing equipment to image and enhance the appropriate cell type and number for each case.

Uses laboratory information system to enter results, look up patient data, make necessary entries, and run any required reports.

Sterile Techniques: Sterile technique and other blood-borne pathogens guidelines are observed in the preparation and handling of all samples. Responsibilities also include the proper cleaning and decontamination of all work areas.

Prepares solutions for FISH washing procedures, equipment set up, slide fixation and slide preparation.

a-CGH Microarray include genomic DNA isolation from whole blood (daily routine), tissues and tissue culture cells, Preparation of Agarose gels and Agarose gel electrophoresis, Restriction digestion of genomic DNA, Enzymatic Labeling of genomic DNA and interpretation of data.

Urovysion by FISH includes detection of aneuploidy for respective chromosomes in urine specimens from persons with hematuria suspected of bladder cancer.

Research Assistant, William Paterson University, NJ, USA

(January 2009-June 2009).

Nitric Oxide as a signaling molecule in Anuran Metamorphosis (2009)

Analyze the role of Reactive Oxygen Species (ROS) [Nitric Oxide as a signaling molecule] in metamorphosis of tadpoles (Xenopus Laevis).

• Immunohistochemistry for localization of NO (Nitric Oxide) and NO (nitric oxide) producing cells.

• Silver staining and Rhodamine 123 staining for localization of mitochondrial rich cells.

• Confocal microscopy for detection of localized cells.

• NADPH-Diapharose histochemistry for detection of mitochondria cells which are the source of NO producing cells in the tail epidermis of whole mounts of Xenopus laevis.

• Responsible for conducting the assays such as Elisa, RT-PCR, Immunohistochemistry, Silver staining and Western Blot techniques independently.

• Statistical analysis of the data obtained.

RESEARCH ASSISTANT (January 2007-December 2007)

Antibody Response in Mice

Evaluation of antibody response to Ectromelia in mice while administering an antiviral drug.

• Elicited immune response in mice model using peptide antigen.

• Analysis of serum for antibody response using ELISA.

Analyst, Sri Venkateshwara Pharmaceuticals, Hyderabad, INDIA

(May 2005 - June 2006).

• Responsible for day-to-day operations including staff supervision.

• Responsible for purchasing of chemicals and chemical inventory management.

Biochemistry Lecturer, Ravindra Degree College, Kurnool, INDIA

(June 2003 - April 2005).

• Taught lecture concepts of Biochemistry (lecture and laboratory) to undergraduate students.

• Preparation of reagents in the qualitative and quantitative analysis of carbohydrates and proteins.

CONFERENCES/PRESNTATIONS:

Presented a poster “Does Nitric Oxide Act as a Signaling Molecule for Morphogenesis in Anuran Intestinal Remodeling?” with Dr.Jaishri Menon in the Annual Symposium 2009 held at William Paterson University, New Jersey.

EDUCATIONAL QUALIFICATIONS

Master of Science in Biology Dec 2008

Saint Louis University, St.Louis, MO, USA GPA 3.70

Masters in Biochemistry May 2002

Nagarjuna University, Andhra Pradesh, India GPA 3.85

Bachelors in Biochemistry May 2000

Sri Krishnadevaraya University, Andhra Pradesh, India GPA 3.89

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