Iris Fontaine-Glover

*** ********** ****

Upper Chichester, PA 19014

484-***-****

sxss0n@r.postjobfree.com

OBJECTIVE

To use my years of laboratory experience to find a permanent position within a cellular biology, molecular biology, biochemistry or related scientific laboratory. I have a well developed track record in various molecular techniques, strong work ethic, dedication to quality and a motivated team player; which would make me an asset to any organization.

EMPLOYMENT

Delaware Public Health Laboratory Smyrna, Delaware

(Contract Position with Lab Support) November 2010- May 2011

Microbiologist 2

Performed Real Time PCR for Influenza Virus, Norovirus, Mumps, and West Nile Viruses. Certified Multiple Loci VNTR Analysis (MLVA) sequencing protocols for two serotypes of Salmonella, E.coli and Listeria bacteria. Certified to run Pulse Field Gel Electrophoresis (PFGE) for Salmonella, Shigella, E.coli and Listeria bacteria. Operated, maintained, calibrated and assisted with troubleshooting computerized and non-computerized laboratory equipment such as automated extraction systems, amplification platforms, (2) types Real Time PCR platforms, centrifuges, incubators, etc. Prepared, standardized and evaluated media and reagents. Developed, validated and implemented plans, methods, standard operating procedures, and quality control and quality assurance procedures. Maintained accurate manual and computerized data systems and laboratory record keeping.

Pennsylvania Department of Health Lionville, Pennsylvania

(Contract Position with PMHC) October 2009 – July 2010

Microbiologist 2

Performed microscopic examinations of laboratory specimens to determine the presence, identity and quantity of bacteria, fungi, protozoa or other microorganisms in any phase of their development. Work required application of scientific techniques to a variety of microbiological tests and examinations in fields such as bacteriology, mycology, virology, serology or parasitology. Technical work was performed with a high degree of independence and responsibility. Work of a Microbiologist 2 is differentiated from the lower level by the independent responsibility for conducting research to develop and modify standard operating procedures, and by performing advanced microbiological analysis through the use of advanced testing procedures; Nucleic Acid Extraction, RT-PCR, Respiratory Viral Screening using Luminex and Direct Fluorescent antibody testing, CD4/CD8 count using B&D Flow Cytrometry FACS Count, Biotage Pyro Sequencing and Viral Cell Culture.

Diagnocure Oncology Laboratories West Chester, Pennsylvania

Sr. Research Technologist February 2008 – August 2008

Used state-of-the art technologies to analyze patient samples and provided test results that helped physicians and patients better understand the risk of recurrence of the patient’s colorectal cancer, and hence make better decisions with regard to potential adjuvant treatment. Primary responsibilities for the Sr. Research Technologist was to set up the molecular biology laboratory, modified existing Standard Operating Procedures to make them more efficient; to manage and train six new and existing laboratory staff; maintaining laboratory supplies and instrumentation at an optimal functional status. The lab consisted of four stations that included: 1.) A station that logged the specimens in the LIMS system; 2.) A histology station that embedded the lymph node tissue, made microtome sections and H&E stained the slides; 3.) A molecular station that performed various molecular biology experiments (RNA extraction, RT-PCR, real-time PCR, mRNA micro-array profiling;4.) Final station that was designed to analyze data produced.

Iris Fontaine-Glover

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Xenogen Biosciences Cranbury, New Jersey

Phenotype Department, Metabolism Group September 2006 - February 2008

Research Technician III, Metabolic Group Team Leader

This group evaluated metabolism in mice pre and post high fat diet. Data from several diagnostic levels, that affect metabolism, are gathered to compare knockout versus wild type groups of mice. The following tests were performed bi-monthly: Adiponectin RIA, Aldosterone EIA, ELISA for: Angiotensin, Free Fatty Acids, Phospholipids, Leptin, Glycogen. Urinalysis (electrolytes, creatinine, and calcium) is done via Wassermann ACE Machine. Other tests and procedures: PIXI-mus X-ray unit for DEXA analysis, X-Rays, Oral Glucose Tolerance Test, Cardiac Bleeds, Retro Orbital Bleeds, IP and subcutaneous injections, Water Retention Study, Oral Gavages and Tissue Collection. The group leader’s responsibilities included: SSOP data analysis, loading data in the client database and making the schedule for testing of the mouse knockout strains.

Xenogen Biosciences Cranbury, New Jersey

Molecular Biology Group October 2003 - August 2006

Research Technician II

Screened genomic DNA of using Polymerase Chain Reaction (PCR), Southern Blot and/or Slot Blot, Taqman real time PCR, and entered results in the database. Extracted genomic DNA from mouse-tail for various projects; Purified RNA from cell cultures samples; Perform basic molecular cloning; Made solutions and ordered supplies; Interpreted and performed new protocols as assigned. Use of Tecan Liquid Handling System.

American Red Cross Blood Services Philadelphia, Pennsylvania

Senior Technologist l April 2001- March 2002

Human Leukocyte Antigen/Molecular Genetics Laboratory

Participated in assuring the smooth daily operation of the laboratory. Performed PCR testing, Southern slot blot SOP, DNA sequencing and antibody screening to cross match HLA alleles of donors and patients. Analyzed southern blot films and assigned molecular HLA Class l and/or Class ll allele /allele codes. Implemented new procedures and became familiar with the various computer software related to data analysis; Assisted in the training of new lab personnel; Participated in providing HLA services for various transplant programs.

University of Pennsylvania School of Medicine Philadelphia, Pennsylvania

Department of Cell and Developmental Biology March 1998 - April 2001

Research Technician

Worked as primary technician in a laboratory that conducted research to study the role a family of winged helix genes have on development of nervous tissue in mice. Worked independently to performed standard molecular laboratory techniques including; gene targeting and cloning for the production of knockouts and transgenic mice; DNA and RNA extraction; RNA expression assays; Protein purification; Library construction and planning; Maintained mouse colony; Genotyped mice via PCR amplification and southern blot analysis; Conducted animal surgery and dissection; Gathered tissue for sectioning and/or slide staining; Performed hormonal injections; Prepared laboratory reagents; Performed behavioral testing (swim, maze, and hot plate tests,) Working as the primary technician involved extra duties such as: input and analyze data, organized and maintained general lab DNA and glycerol stocks; maintained and ordered supplies; communicated with researchers & other staff regarding ordering and shipment of mice; solely responsible for Radioactive handling and safety, maintaining lab safety; training seven new hires and students volunteers.

Iris Fontaine-Glover

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QUALIFICATIONS/ PRIMARY LABORATORY SKILLS

Mouse embryo micro-dissection; Mouse handling and husbandry (including maintenance and organization of mating colonies, genotyping, injections.) Standard molecular biology techniques including: gene targeting and expression, PCR, RT-PCR, southern and northern blotting, capillary array sequencing, use of visible and UV spectrophotometer. Comfortable working on in-vivo studies; Basic Tissue Culture techniques; Histological techniques including whole mount in situ hybridization, x-gal staining, immounohistochemistry, tissue embedding, and sectioning; Standard microbiology techniques including isolation, culture and media preparation. Radioactive handling, maintaining lab safety. Demonstrated record of consistency and accuracy working in a technical environment; excellent organizational, record-keeping skills; Proficient verbal and written communication skills and presentation skills; Proficient Computer skills.

Instruments and Platforms:

Applied Bio Systems: PCR Machine; 7900, 7700, 7500 Real-Time PCR Machines ; ABI PRISM 373 DNA Sequencer

Beckman Coulter: CEQ Sequencer 8000

Bio Rad: Bio Rad My Cycler thermal cycler CFX96

Cepheid: Smart Cycler

Eppendorf: epMotion Nucleic Acid Extraction Station; PCR Machine; Master Cycler Gradient

Qiagen: Qiagen Bio Robot MDx Workstation; Pyro Mark Q24

Tecan: Liquid Handling & Robotics

Roche: Magna Pure Extraction Workstation; Light Cycler 2.0; 480 Real-Time PCR System Light Cycler

Xenogen: IVIS-200 Optical In Vivo Imaging System

EDUCATION

University of Pittsburgh Pittsburgh, Pennsylvania

B.S. Biology, Minor in Chemistry April 1994

PUBLICATIONS

Kloetzli, J.M., Fontaine, I.A., Brown, E.R., Kuo, M. and P.A. Labosky. (2000).

The winged helix gene, Foxb1, controls development of both mammary glands and regions of the CNS that regulate the milk-ejection reflex.

http://www3.interscience.wiley.com/cgi-bin/abstract/76510947/START Genesis 29:60-71.

Hanna, L.A., Foreman, R.K., Fontaine, I.A., Tarasenko, I.A., Kessler, D.S. and P.A. Labosky. (2002). Requirement for Foxd3 in establishing pluripotent stem cells of the early mouse

embryo. http://www.genesdev.org/cgi/content/full/16/20/2650 Genes and Dev. 16:2650-2661.

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