Manager Design Engineer
Resume:
Allen Zillmer
Fort Mill, SC **707
Phone: +1-803-***-****
E-mail: s5o195@r.postjobfree.com
Career Summary
Evolved the field of genomics by developing and deploying platforms devoted to the analysis of botanical, microbial, human and other
mammalian organisms. Contributed to the future of medical research and gene therapy by developing vectors for the analysis and
treatment of terminal diseases.
Research Work Experience
Staff Scientist –Genomics Laboratory, April 2009 – May 2011
David H. Murdock Research Institute – Kannapolis, NC
http://www.dhmri.org
As Staff Scientist of the Genomics Laboratory I deployed and optimized numerous platforms devoted to genotyping, gene expression,
copy number variation, methylation analysis and other genomic applications within DHMRI at the North Carolina Research Campus.
Brought together the lab’s critical information by designing and implementing a web based LIMS/ELN system. I collaborated with the
academic community as well as private industry on large and small projects alike. Over the past two years I have been heavily involved
in the experimental design, production and data analysis of multiple $100K+ projects devoted to understanding of the genomic basis of
multiple sclerosis, autism, muscular dystrophy, osteoarthritis, cardiovascular disease, acne, cancer, fatty liver and other human diseases.
Related Techniques/Instruments
• Genotyping, Expression Analysis & Methylation Analysis / Illumina-iScan
• Liquid Handling Robotics / Tecan Freedom Evo, Thermo Matrix, Qiagen Universal, Bio-Mek NX
• Gene Expression & Genotyping / Affymetrix GeneChip Scanner 3000 & Fluidics Station
• Targeted Gene Expression, Genotyping & Methylation Analysis / Sequenom-MassArray Compact, RS1000 Nano-Dispenser &
Bruker MALDI-TOF-MS
• Genotyping, SNP Validation, CNV Analysis, library quantification, Methylation Analysis / ABI 7900HT qPCR
Vector Design Engineer - McColl-Lockwood Muscular Dystrophy Laboratory- December 2003 – November 2007
Carolinas Healthcare System. – Charlotte, NC
http://www.carolinasmedicalcenters.com
As vector design engineer of the McColl-Lockwood Muscular Dystrophy team I designed and constructed vector systems for the study of
and eventual therapy of Becker, Duchenne and Limb-Girdle muscular dystrophies with a focus on the FKRP, Dystrophin and alpha-
Dystroglycan genes. Some of these vectors are still being used to this day to further the knowledge and treatment of Muscular
Dystrophies.
Related Techniques/Equipment
• Plasmid Vector Design & Construction
• Gene synthesis
• Nucleic Acid Isolation & Purification
• Site-Directed Mutagenesis
Research & Development Manager- Membrane Separations Technology- December 1997 – February 2003
Separation Technology Inc – Rock Hill, SC
As Research & Development Manager, I developed separations processes for industry by leveraging thin-film membrane technology
such as reverse osmosis, ultra -, micro-, and nano-filtration systems. I performed more than 100 failure analyses on thin-film composite
membrane modules for the chemical synthesis industry and municipalities. Membrane technology has grown exponentially in recent
years in the development of new and exciting products. In my tenure at Separation Technology I was involved in the development of the
first organic light emitting diodes (OLED), which has burst onto the scene in recent months as a leap forward in display technology.
Related Techniques/Equipment
• Environmental Scanning Electron Microscopy/Philips Electroscan 2020 ESEM
• Fourier Transform Infrared/Thermo FTIR
• Energy Dispersive X-Ray Analysis
• Programmable Logic Controllers
Education
• M.S. Molecular Biology: Winthrop University, Rock Hill, SC. “Microbial Characterization of an Industrial Petroleum Hydrocarbon
Bioremediation System”
• B.S. Biology: Winthrop University, Rock Hill, SC
Data Analysis and Miscellaneous Software
• Proficiency in both Windows-based (Win7) and Mac-based (OS X) environments
• Genotyping & Gene Expression Analysis (Illumina-GenomeStudio, ArrayStar, Agilent-GeneSpring, CLC Genomics
Workbench, Affymetrix-Expression Console)
• Vector Design (MacVector, DNA Star, CLC Genomics Workbench)
• Productivity (Photoshop, Illustrator, Premiere Pro, Vegas 9.0, After Effects, Dreamweaver)
• Microsoft Office Suite (Word, Excel, PowerPoint, Access, Outlook, One Note, Entourage)
Presentations
Screening of Antisense Oligonucleotides for Human Dystrophin Exon Skipping in a GFP Reporter Culture System with
High Sensitivity and Specificity.
Ehsan Benrashid, Saafan Malik, Allen Zillmer, Randy J. Thresher, Jeffrey Rosenfeld, Qi Long Lu.
American Society of Gene Therapy (ASGT) Seattle. WA, 2007
Antisense Oligonucleotide Selection for Exon Skipping by a Readout Reporter System with High Capacity and Specificity
Allen E. Zillmer, Qi L. Lu, Jeffrey Rosenfeld.
American Society of Gene Therapy (ASGT) Seattle. WA, 2007
Publications
Use of Mutant-Assisted Gene Identification and Characterization (MAGIC) to identify novel genetic loci that modify the
maize hypersensitive response.
Chaikam V, Negeri A, Dhawan R, Puchaka B, Ji J, Chintamanani S, Gachomo EW, Zillmer A, Doran T, Weil C, Balint-Kurti P, Johal G.
Theor Appl Genet. 2011 Oct;123(6):985-97. Epub 2011 Jul 27.
Targeted skipping of human dystrophin exons in transgenic mouse model systemically for antisense drug development.
Wu B, Benrashid E, Lu P, Cloer C, Zillmer A, Shaban M, Lu QL.
PLoS One. 2011;6(5):e19906. Epub 2011 May 17.
Guanine analogues enhance antisense oligonucleotide-induced exon skipping in dystrophin gene in vitro and in vivo.
Hu Y, Wu B, Zillmer A, Lu P, Benrashid E, Wang M, Doran T, Shaban M, Wu X, Lu QL.
Mol Ther. 2010 Apr;18(4):812-8. Epub 2010 Jan 19.
Mutations alter secretion of fukutin-related protein.
Lu PJ, Zillmer A, Wu X, Lochmuller H, Vachris J, Blake D, Chan YM, Lu QL.
Biochim Biophys Acta. 2010 Feb;1802(2):253-8. Epub 2009 Nov 10.
Fukutin-related protein localizes to the Golgi apparatus and mutations lead to mislocalization in muscle in vivo.
Keramaris-Vrantsis E, Lu PJ, Doran T, Zillmer A, Ashar J, Esapa CT, Benson MA, Blake DJ, Rosenfeld J, Lu QL.
Muscle Nerve. 2007 Oct;36(4):455-65.
Zillmer, A. Microbial Characterization of an Industrial Petroleum Hydrocarbon Bioremediation System.
Masters Thesis. 2002
Personal / Miscellaneous Information
• Enjoy reading, cooking, wine, photography, International travel & aviation
• Relocation possible anywhere
• US Citizen
References
Dwight Dimaculangan, Ph.D.
Director of Undergraduate Research for Winthrop University
Interim Chair of the Biology Department
Professor of Biology
803-***-****, ext. 6443
s5o195@r.postjobfree.com
Jonathan Salo, MD, FACS.
Surgical Oncologist and faculty member of the Department of General Surgery, Division of Surgical Oncology
Carolinas HealthCare System
s5o195@r.postjobfree.com
Qi Long Lu, MD, Ph.D.
Research Director- McColl-Lockwood Laboratory for Muscular Dystrophy Research at Carolinas Medical Center
Carolinas HealthCare System
s5o195@r.postjobfree.com
Yankai JIA Ph.D.
GENEWIZ Inc.
115 Corporate Blvd
South Plainfield, NJ07080
s5o195@r.postjobfree.com
Tim Stinchcombe Ph.D.
Director-Genomics Analysis
Immune Tolerance Institute
s5o195@r.postjobfree.com
Chad Dow
Field Application Scientist
Affymetrix
s5o195@r.postjobfree.com
Amr Ragab, MS
Manager Metabolomics
David H. Murdock Research Institute
s5o195@r.postjobfree.com
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