Scientist / Manager Scientist/biologist

Yongqing Wang, Ph.D.

***** ******* **. ****: 615-***-****

Potomac, MD 20854 ************@*******.***

SUMMARY OF QUALIFICATIONS

Broad knowledge of animal model, molecular and cell biology, stem cell, histology;

Ability to troubleshoot protocols and analyze data;

Having the ability to work independently and as a member of a team.

SKILLS

DNA: Isolation, purification and endonuclease analysis of chromosome and plasmid from tissue, cell and bacteria, DNA subclone and site-specific mutagenesis. DNA transformation to bacteria, DNA transfection and transient gene expression in mammalian cells. DNA sequencing, Southern blot, in situ hybridization, ultra centrifugation, DNA fragment purification from agarose gel, PCR techniques (amplification of genomic and fragment DNA, RT-PCR, etc), design and synthetic oligonucleotide primers.

RNA: RNA isolation, purification and analysis, reverse transcription, northern blot, in vitro transcription, double-stranded RNA preparation and injection.

Protein: Expression of cloned genes, detection and analysis of proteins expressed from cloned genes, western blot, protein isolation and purification.

Immunological Techniques: Immunohistochemistry, immunofluorescence, tissue microarray, immunocytochemistry, fluorescence microscopy, immunoprecipitation, ELISA.

Cell and Molecular Biology: primary epithelial and fibroblast cell culture from the transgenic mouse tumor. Generate, characterize and maintain stable mammalian cell lines, cell cloning, migration assay, transfection and retrovirus infection, FACS analysis, RNAi, luciferase assay, Microarray.

Stem Cell: mouse embryonic stem cell culture and manipulation, isolated cancer stem cell by sorting cancer cells.

Animal Model: Direct experience with in vivo models of cancer, such as tissue recombination, prostate tissue rescue, xenograft and orthotopic tumor models. Animal surgery and microsurgery, injections (subcutaneous, intraperitoneal, intravenous), maintaining and managing transgenic mouse strains, animal handling, dissection, sample collection. Extensive experience with general animal tissue processing, embedding, sectioning, histology and immunohistochemical staining.

Biostatistics and Bioinformatics: t-test, ANOVA, repeated measure, Pairwise and multiple sequence alignment, phylogenetic tree construction.

Programs: SAS Programming, Word, Excel, PowerPoint, Photoshop.

EDUCATION

Ph.D. Institute of Zoology, Chinese Academy of Sciences (CAS), Beijing, China 2000

M.S. Department of Biology, Shandong University, Jinan, China 1994

B.S. Department of Biology, Anhui Normal University, Wuhu, China 1991

RESEARCH EXPERIENCE

Senior Scientist 2007-present

Washington Biotechnology Inc. Baltimore, MD

Manage cancer-related projects and Pharmacokinetic/Pharmacodynamic projects.

Supervise technicians to do animal Pharmacokinetics, toxicity and compounds testing in various disease models for efficacy.

Manage and conduct xenograft, orthotopic and custom xenograft animal models.

Write Animal Study Proposals, provide recommendations for study design to clients.

Analyze data using SAS program and write reports.

Research Fellow 2001-2007

Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN

Established and characterized three mouse prostate NeoTag epithelial cell lines that are androgen responsive. In tissue recombinants, the three NeoTag strains developed PIN lesions and/or more extensive adenocarcinoma than seen in the original mouse prostate model.

Functional analysis of p120 in prostate tumor progression and possibly metastasis in mouse prostate NeoTag cell lines.

Created a novel model utilizing mouse embryonic stem cells in tissue recombination with embryonic mesenchyme to develop prostate-like tissue

Functional study of androgen independent prostate cancer by cultured neuroendocrine cancer cell disclosed that NE secretions combined with low levels of androgens activated the AR, which can be a mechanism that allows prostate cancer to escape androgen ablation therapy

Analyzed gene expression of HNF-3α, an endoderm developmental factor and essential for androgen receptor (AR)-mediated prostatic gene activation, in over 300 prostate cancer patient samples.

Characterized gene regulation of tumors arising from the prostates of “Lady” LPB-Tag transgenic models using microarray.

Established orthotopic model to study metastatic potential of human prostate cancer.

Research fellow 2000-2001

Department of Microbiology, Meharry Medical College. Nashville, TN

Mapped initiation sites for DNA replication in (in vivo) human chromosomes around a 5 kb region encompassing ARSH1 (a 1.1-kb human DNA fragment) by a nascent strand DNA abundance assay.

Graduate Student 1997-2000

Department of Population Biology, Institute of Zoology, Chinese Academy of Sciences (CAS)

Studied the genetic polymorphism of central and peripheral populations of rat-like hamster (Cricetulus triton).

Graduate Student 1991-1994

Department of Biology, Shandong University

Analyzed chemical components of castoreum of Xinjiang-Mongolia beaver (Castor fiber birulai Serebrennikov).

HONORS AND AWARDS

AACR Scholar-in-Training Award, Innovations in Prostate Cancer Research Conference 2006

Chinese Second Prize in the National Natural Science Award 2003

Chinese Academy of Sciences Second Prize for Scientific Achievement 2001

PROFESSIONAL AFFILIATIONS

China Zoological Society (CZS)

American Society for Basic Urological Research (SBUR)

American Association for Cancer Research (AACR)

American Society of Human Genetics (ASHG)

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