High School University

Curriculum vitae

NAME: Sepulveda, R. Tomas

Address: **** *. *** **. ****** AZ, 85710.

E-mail: ***********@*****.***

Phone: (C) 520-***-****

EDUCATION:

Institution Degree Date Field of Study

University of Arizona Ph.D. 2005 Microbiology & Immunology

(Tucson, Arizona)

National Polytechnic Institute M.Sc. 1994 Immunology

(Mexico City, Mexico)

University of Sonora B.Sc. 1989 Biochemistry

(Sonora, Mexico) in Clinical Lab Sci

Doctoral dissertation title: T-Cell Receptor V 8.1 Peptide Reduces Coxsackievirus Induced Cardiopathology During Murine Acquired Immunodeficiency Syndrome and Aging.

Master of Science dissertation title: Antigenic Protein Analysis of Mycobacterium tuberculosis isolates and M. tuberculosis Ra and Rv with homologous and heterologous serum samples.

Bachelor of Science dissertation title: CA-125 antigen for the diagnosis of breast cancer.

Other Languages: Spanish.

EXPERIENCE:

Administrative Experience: As Department Chair of Education at the Tohono O’odham Community College, duties include: administrative, budget managing, schedule coordinator, hiring, grants project director. Other activities include participation on advisory committees, teaching responsibilities, coordinating departmental efforts in the implementation of goals and objectives as well as policies and procedures, selection of adjunct faculty, coordinating efforts in training and mentoring of new faculty. Coordinating the selection of instructional support equipment, materials, maintenance, services, supplies, facilities planning and staffing needs. Coordinating student recruitment and retention initiatives. Coordinating certification of faculty, scheduling, tutoring. Coordinating departmental curriculum, program development and review. Coordinating department activities in articulating academic programs. Assisting in development of departmental budgets. Also, assisting in department staffing needs and evaluation of candidates for new faculty positions. Assisting in the writing of grant proposals for Department of Education, Department of Defense.

Teaching: Classes and lab included: Leaderships Skills, Study Skills, Biology, Microbiology, Medical Bacteriology, Biochemistry and Immunology. Other activities included advising students on academic goals as well as requirements for schedule and graduation. In charge of the University Clinical Laboratory for medical laboratory tests for the indigent.

Major Research Fields: Drug and alcohol abuse: Immunomodulation and AIDS, (B) Immune and nutritional restoration in a murine retroviral model of AIDS, (C) Immune mechanisms of heart disease in AIDS, (D) Infectious diseases: tuberculosis, retroviral infections, coxsackievirus (heart disease and Type 1 Diabetes).

Lab techniques experience: Western blot, Hybridization; In vitro amplification of DNA by the Polymerase Chain Reaction; Real time Polymerase chain Reaction; Extraction, Purification and analysis of mRNA from eukaryotic cells for Microarray analysis; Immunocytochemistry of frozen and paraffin tissue section; Histochemistry H&E; Cytokine determination w/ELISA; Cytokine array; Cell Proliferation (T and B Lymphocyte mitogen-induced response); Immunomagnetic cell separation; Identification of leukocyte subsets by multiparameter flow cytometry; Gel electrophoresis ; Bacteria cell cultures frequently used in research: Enterobacteriaceae, Mycobacteriaceae, Streptococcaceae, Micrococcaceae and Fungi; Growth and Purification of Murine Leukemia Virus; Growth and Purification of Coxsackievirus; Phlebotomy: human, mouse and rabbit; Production of polyclonal antibodies in rabbits; Purification of Immunoglobulins, Hematology differential, Urinalysis, Blood Chemistry.

Computer training: Excel, Power Point, Flash Animations, Dream Weaver, WebCT, Website and Blog Design, Ovid, Medline, Basic hardware installation, Wireless network installation.

RESEARCH AND PROFESSIONAL EXPERIENCE:

High School Teacher, College Fray Pedro de Gante,

H. Nogales, Sonora, Mexico

(Physics, Mathematics and Biology) August, 1988- Jun, 1990

High School Teacher, College La Salle

H. Caborca, Sonora

(Physics, Mathematics and Biology) January, 1990- April, 1992

Research Assistant, National Polytechnic Institute,

Mexico City, June, 1992 –August, 1994

Professor, University of Sonora,

Sonora, Mexico.

(Immunology, Medical Microbiology, Microbiology) June, 1990-July, 1992

August, 1994 – May, 1997

Research Assistant, University of Arizona,

Tucson, Arizona May, 1997 –July, 1999

Research Specialist, University of Arizona,

Tucson Arizona July, 1999- July, 2004

Volunteer as lab assistant. Various labs: University

of Arizona, Tucson AZ January, 2005-January, 2007.

Certified Neurolinguistic Programing Therapist December, 2009.

Westland Institute.

Certified Clinical Hypnotherapist. Westland Institute March, 2010.

Department Chair of Education,

Tohono O’odham Community College April, 2007-June 2011

Member of the VA Privacy Board June 2010- April 2011

AWARDS

• Graduated with Honors from the University of Sonora, Hermosillo, Sonora, Mexico.

• Advisor for the High School Team of Physics of the Caborca University Center La Salle, winning fist place in the La Salle’s Third week of Youth. May,1991. Caborca, Sonora, México.

• Marshall Foundation Graduate Scholarship from 2000-2002.

• Grant Award from the Graduate College Administration Sponsored Projects Services of the University of Arizona, No. 322A for the project: Cardiopathology in Murine AIDS. April, 2001.

• American Association of Immunologist Minority Scientist Travel Award. May, 2003.

• Award by the Procter and Gamble Research & Technical Careers in Industry Conference (RTCI). Cincinnati, Ohio. June, 2003.

• Research Advisor and Mentor to Randy Vazquez in the research project: “Immune Enhancing Effect of TCR V 8.1 peptide in Activated T cells”. Presented in the 2003 Minority Health Disparities Summer Research Program at the University of Arizona. Awarded Winning Research Project.

Research Training/Mentoring. Responsible for advising and mentoring graduates students with research projects.

Clifford Allan Gross, 2001.

Ross Varga, 2002.

Thomas Eagan, 2002.

Randy Vazquez, summer 2003.

Sherma Shibaldi, 2005.

Project Director for the Emergency Preparedness Grant: collaboration TOCC (Tohono O’odham Community College) and College of Public Health/University of Arizona; 2007-2009.

Project Director for the Summer College Bridge Program/STEM grant (Science Technology and Mathematics); 2009-2011.

Lead faculty for the Summer College Bridge Programs 2007, 2008 and 2009. Programs include: PREP 101 (Reading, Writing and Mathematics) and STEM 101 (Mathematics, Computer Science, Physics and Biology)

TRAINING

Course in: Techniques and Activities for Teaching and Learning. University of Sonora, Mexico.

Course in: Clinical Biochemistry, University of Sonora, Mexico.

Workshop in: Computer Viruses, University of Sonora, Mexico.

Workshop in: Laboratory Diagnosis of Brucelosis, Mazatlan, Sinaloa, Mexico.

Course in: Elements for Design and Analysis of Research Projects

Course in: Human Genetics, National School for Biological Sciences at the National Polytechnic Institute in Mexico City, Mexico.

Course in: Leishmaniasis in the New World, National School for Biological Sciences at the National Polytechnic Institute in Mexico City, Mexico.

Workshop in: Histocompatibility, National Institute of Diagnosis and Epidemiology in Mexico City, Mexico.

Course in: Molecular Genetics, National Institute of Diagnosis and Epidemiology in Mexico City, Mexico.

Course in: Cytokines, Department of Immunology, National School for Biological Sciences at the National Polytechnic Institute in Mexico City, Mexico.

Workshop in: Medical Mycology, Department of Microbiology, National School for Biological Sciences at the National Polytechnic Institute in Mexico City, Mexico.

Workshop in: Environmental Mutagenesis, Carcinogenesis and Teratogenesis, at the Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.

Workshop in: Laboratory Techniques for Mycobacterium tuberculosis and Mycobacterium leprae, National Institute of Diagnosis and Epidemiology in Mexico City, Mexico.

Workshop in: Microsoft Access 2.0 (Basic). North Regional Unit, University of Sonora, Mexico.

Workshop in: Excel 4.0. North Regional Unit, University of Sonora, Mexico.

Blood Born Pathogens Training. University of Arizona, Tucson, AZ, USA

Certification on: Laws and Regulations, Surgery and Anesthesia, Rodents, Hamsters and Guinea Pigs. University of Arizona, Tucson, AZ, USA

Radiation Protection Certification. University of Arizona, Tucson, AZ, USA.

Laboratory Safety Certification. University of Arizona, Tucson, AZ, USA.

Certification program on Rodents, Hamsters and Guinea Pigs, Zoonotic Diseases of Rodents and Rabbits. University of Arizona, Tucson, AZ, USA.

Laboratory Safety Training. University of Arizona, Tucson, AZ, USA.

Workshop: Developing program opportunities between the Department of Homeland Security and the Tribal Colleges and Universities. Arlington, Virginia, USA, 2007.

Workshop for Tribal Colleges and Universities. Awareness, Access and Opportunity. Albuquerque, New Mexico, 2007.

Tohono O’odham Community College liaison for the Foundations of Excellence; 2007-2008.

AIHEC Evaluation Workshop in Tucson Az. November 19-21, 2009.

SPEAKER AT SEMINARS AND LECUTRES

Antigenic characterization of Mycobacterium tuberculosis. Department of Chemical and Biological Sciences. University of Sonora, Mexico, October 5, 1994.

Antigen recognition of Mycobacterium tuberculosis isolates. North Regional Unit, University of Sonora, Mexico. October 27, 1994.

Antigenic characterization of Mycobacterium tuberculosis H37Rv and H37Ra. Veracruz, Veracruz, Mexico. April 6, 1995.

The Immune Response. Caborca, Sonora, Mexico. January 15,

1995 (20 hour course).

Molecular Aspects of the Immune Response. University of Sonora, Mexico. 5 – 16 of December, 1994 (20 hour course).

Acquired Immunodeficiency Syndrome. North Regional Unit, University of Sonora, Mexico. April 19, 1995.

Immunoparasitology. University of Sonora, Mexico. July 3, 1995

Update on HIV. North Regional Unit, University of Sonora, Mexico. October 27, 1995.

Autoimmunity. State Children’s Hospital. Hermosillo, Sonora, Mexico. April 26, 1996.

Flow Cytometry Determination of HLA-DR, CD9 and CD63 in platelets of patients with autoimmune thrombocytopenic purpura. Northwest National Medical Center. Third Meeting of Health Research. Obregon, Sonora, Mexico. April 10, 1996.

Immune Response to Mycobacterial antigens. Northwest National Medical Center. Third Meeting of Health Research. Obregon, Sonora, Mexico. April 10, 1996

Autoimmune Thrombocytopenic Purpura. 1st Symposium of Scientific Research. Hermosillo, Sonora, Mexico. April 11, 1996.

Updates in Immunology. State General Hospital, Hermosillo, Sonora, Mexico. January 26, 1996.

Bovine Spongiform Encephalitis. North Regional Unit, University of Sonora, Mexico. May 16, 1996.

Workshop : Flow Cytometry Applications. University of Sonora, Mexico and University of Arizona, Tucson, Arizona, USA. 20 – 31 May, 1996 (20 hours).

Cells of the Immune Response. University of Sonora, Hermosillo, Sonora, Mexico. June 20, 1996.

Cellular Immunity and Cytokines. University of Sonora, Hermosillo, Sonora, Mexico. June 24, 1996.

Induction of the Immune Response and Antigen Processing. University of Sonora, Hermosillo, Sonora, Mexico. June 21, 1996.

Hypersensitivity. University of Sonora, Hermosillo, Sonora, Mexico. June 25, 1996.

Basic Immunology (20 hours course). State General Hospital. Obregon, Sonora, Mexico. October 12, 1996.

First, Second and Third Generation Vaccines. University of Sonora, Mexico. December 6, 1996.

Antigen Presentation. University of Sonora, Mexico. January 17, 1997.

Delayed Type Hypersensitivity in Guinea Pigs Immunized with Mycobacterial antigens and Challenged with Mycobacterium Tuberculosis Antigenic Fractions. Fourth Meeting of Health Research. CIAD, Hermosillo, Sonora, Mexico. April 10, 1997.

Identification of Mycobacterium avium in Waste-water Using the Polimerase Chain Reaction. Fourth Meeting of Health Research. CIAD, Hermosillo, Sonora, Mexico. April 11, 1997.

Lecturer for the Myasthenia Gravis Support Group. “Understanding Myasthenia Gravis”. Tucson, Arizona. January, 1999. Jan Levecchio, Program Coordinator. Myasthenia Gravis Support Group 520-***-****.

Lecturer for the Kokopelli Rotary International Club. “Aging and the Immune Response”. Tucson, Arizona. August 7, 2003.

Lecture for the Myasthenia Gravis Support Group. “Updates on Myasthenia Gravis”. Tucson, AZ. October, 2005. Jan Levecchio, Program Coordinator of the Myasthenia Gravis Support Group 520-***-****.

Lecture for the Association of Chemical-Biologist. “Immunobiology”. Nogales, Sonora, Mexico. December 2nd, 2005. Ernesto Pacheco, Program Coordinator of the Association. ***************@*******.***

Lectures for the Association of Chemical-Biologist: “Coxsackievirus induced Cardiopathology” and “Avian Influenza”. Nogales, Sonora, Mexico. June 30th, 2006. Ernesto Pacheco, Program coordinator of the Association. ***************@*******.***

Lecture: The Immune System; to Certified Nurses Assistants and Nurses of Archie Hendricks Elder Care Home. Tohono O’odham Nation. May, 2008.

Lecture: How behavior influences biology. Tohono O’odham Community College. March, 2011.

Lecturer at Higher Learning Commission of North America every year from 2007-2011. Topics related to: Education in Tribal Colleges.

PUBLICATIONS:

Lee, J., Sepulveda, R.T., Jiang, S., Zhang, Z., Inserra, P., Zhang, Y., Saeed, H. and Watson, R.R.(1999) Immune Dysfunction During Alcohol Consumption and Murine AIDS: The Protective Role of Dehydroepiandrosterone Sulfate. Alcoholism: Clinical and Experimental Research. 23.5:856-862.

Sepulveda, R.T., Jiang, S., Beischel, J., Bellamy, W.T. and Watson, R.R. (2000) Cocaine Injection and Coxsackievirus B3 Infection Increase Heart Disease During Murine AIDS. Journal of Acquired Immune Deficiency Syndromes. 25:S19-S26.

Marchalonis, J.J., Robey, I.F., Edmundson, A.B., Sepulveda, R.T. and Watson, R.R. (2001) T-Cell Receptor-Derived Peptides in Immunoregulation and Therapy of Retrovirally Induced Immunosuppression. Critical Reviews in Immunology. 21:57-74.

Hosseini, S., Lee, J., Sepulveda, R.T., Rohdewald, P. and Watson, R.R. (2001) A Randomized, Double-blind, Placebo-controlled, Prospective, 16 week Crossover Study to Determine The Role of Pycnogenol In Modifying Blood Pressure In Mildly Hypertensive Patients. Nutrition Research. 21:1251-1260.

Sepulveda, R.T., Jiang, S., Besselsen, D. and Watson, R.R. (2002) Alcohol Consumption During Murine Acquired Immunodeficiency Syndrome Accentuates Heart Pathology due to Coxsackievirus. Alcohol & Alcoholism. 37.2:157-163.

Sepulveda, R.T., Zhang, J. and Watson, R.R. (2002) Selenium Supplementation Decreases Coxsackievirus Heart Disease During Murine AIDS. Cardiovascular Toxicology. 2.1:53-61.

Liu, Y., Montes, S., Zhang, D., Sepulveda, R.T., Yu, Q., Zhang, J., Larson, D.F. and Watson, R.R. (2002) Cocaethylene and Heart Disease During Murine AIDS. International Immunopharmacology. 2:139-150.

Hosseini, S., Sepulveda, R.T., Lee, J. and Watson, R.R. Alcoholism and Immune Function. In Psychoneuroimmunology. Edited by: Ader, R., Felten, D.L. and Cohen, N. 66: 687-700.

Sepulveda, R.T. and Watson, R.R.(2002) Treatment of Antioxidant Deficiencies in AIDS Patients. Nutrition Research. 22:27-37.

Sepulveda, R.T., Watson, R.R. (2002) Alcohol's accentuation of AIDS' nutritional and immune damage, a cofactor in heart disease. In Alcohol and Heart Disease. (Ed. Watson, RR, Myers AK). Taylor & Francis, Chapter 22:250-258.

Sepulveda, R.T., Marchalonis, R.T. and Watson, R.R.(2003) T-Cell Receptor Vb8.1 Peptide Reduces Coxsackievirus Induced Cardiopathology during Murine AIDS. Journal of Cardiovascular Pharmacology. 41(3):489-497.

Sepulveda, R.T., and Watson, R.R. (2003). Immune control of coxsackievirus-induced cardiopathology. Cell Mol Biol. 49(2): 171-80.

Sepulveda, R.T. and Watson, R.R. (2004) Nutrients and Treatment of Heart Disease in AIDS. In AIDS and Heart Disease. ED. Marcel Dekker. (13):187-196.

Beischel, J., Larson, D.F., Yu, Q., Yang, B., Sepulveda, R.T., Kelley, T., Watson, R.R. (2004) Dilated Cardiomyopathy in Retrovirally Infected Mice: A Novel Model for Silent Viral DCM? Cardiovascular Toxicology 4(4):317-326.

Sepulveda, R. T., Marchalonis J.J. and Watson R.R. (2005) T-Cell Receptor V 8.1 Peptide Reduces Coxsackievirus-Induced Cardiopathology in Aged Mice. Cardiovascular Toxicology 5(1):21-28.

Marchalonis, J.J., Schluter, S.F., Sepulveda, R.T., Watson, R.R., and Larson, D. (2005) Immunomodulation by Immunopeptides and Autoantibodies in Aging, Autoimmunity, and Infection. Ann NY Acad Sci . December (1057): 247-259.

Sepulveda, R. T. T-cell receptor Vbeta8.1 peptide reduces coxsackievirus induced cardiopathology during murine acquired immunodeficiency syndrome and aging (Paperback). ProQuest / UMI (March 20, 2006). ISBN-10: 054*******; ISBN-13: 978-0542330957.

ABSTRACTS

(Scientific conferences and meeting)

Sepúlveda, R.T., Estrada-Parra, S., Estrada, I., Escobar-Gutierrez, A., and Amezcua, M.E. Characterization of antigens of the total extract and lipid-free extract of Mycobacterium tuberculosis strain H37Rv developed with sera of patients with active pulmonary tuberculosis.IV Latin American Congress of Immunology and XI Mexican Congress of Immunology. March 3-7, 1996, Zacatecas, Zac, Mexico.

Sepúlveda, R.T., Robles de la Pena, P., Sotelo, N., Carolus, B., and Sanchez-Lucero, M. Determination of HLA-DR and CD9 in platelets of patients with acute and chronic ITP. .IV Latin American Congress of Immunology and XI Mexican Congress of Immunology. March 3-7, 1996, Zacatecas, Zac, Mexico.

Jiang, S.G., Solkoff, D., Adjei, A.A., Inserra, P., Zhang, Z., Sepulveda, R.T., Enriquez, F.J., and Watson, R.R. Effects of Dehydroepiandrosterone Sulfate (DHEAS) on aged mice infected with Cryptosporidium Parvum. FASEB J. 13:483.21, (A640)1999.

Lee, J., Sepúlveda, R.T., Jiang, S., and Watson, R.R. Reduced micronutrient intake accentuates immune dysfunction induced by murine AIDS. FASEB J. 13:457.9 (A590). 1999.

Sepúlveda, R.T., and Watson R.R. Alcohol Consumption During Murine AIDS Accentuates Heart Pathology Due to Coxsackievirus. Experimental Biology, 2001. Orlando Fl. March 31-April 4, 2001. 498.7.

Sepúlveda, R. T., Varga, R., Slayback, D., and Watson, R. R. Host Gene Regulation During Murine AIDS and TCR V 8.1 Peptide Therapy. Submitted to the 2003 Immunology Meeting, Denver Colorado May 6-10. Abstract Number 154.8.

Watson, R.R., Sepúlveda, R.T., Slayback, D., and Marchalonis, J.J. TCR V 8.1 peptide Treatment in Coxsackievirus Induced Cardiopathology In Aged Mice. Submitted to the 2003 Immunology Meeting, Denver Colorado May 6-10. Abstract Number 174.7.

Slayback, D.L., Sepúlveda, R.T., Watson, R.R. Dehydroepiandrosterone sulfate (DHEAS) restores resistance to coxsackievirus in retrovirus infected mice. Submitted to the 2003 Immunology Meeting, Denver Colorado May 6-10. Abstract Number 32.17.

Vazquez, R., Sepulveda, R.T., Watson, R.R. and Marchalonis, J.J. Immune Enhancing Effect of the Novel TCR V 8.1 Peptide in Activated T cells. Minority Health Disparities Summer Research Program. Minority Access to Research Careers and McNair Achievement Program Summer Research Institute. The University or Arizona, Graduate College. Tucson, Arizona. August 4-6, 2003.

Contact this candidate