Microbiologist

CV for the post of Research Lab Position

Summary

Highly motivated and innovative molecular biologist with specialization in cell biology, microbiology, and biochemistry. Expertise in development and execution of microbiology and molecular biology-based research methods. Passionate about learning new scientific skills. Enthusiastic team player adept at providing leadership while also learning from fellow team members. Experienced in managing multiple projects simultaneously.

Professional Qualifications:-

Name of Institution: Shadan Degree College –

Name of course: Microbiology, Zoology, Chemistry [MZC]

Duration: 03 Years

Course Date Month & Year: June 1993 - Mar 1996.

Date of Graduation: April – 1996.

Post Graduate Qualifications:-

Name of Institution: University of East London

Name of course: Molecular Medical Microbiology

Duration: 01 Years

Course Date Month & Year: Feb 2009 – Aug 2010.

Date of Graduation: September – 2010

Research Skills: -

• Proteomics techniques like MALDI-TOF, LC-MS-MS, 1D and 2D-PAGE, whole gel electro-elution, multi-elution, Western blotting, N-terminal sequencing.

• Protein purification techniques like gel filtration chromatography etc.

• Handling of pathogenic (M. tuberculosis) and non-pathogenic cultures.

• Immunological techniques- Flow cytometery, ELISPOT, ELISA, western immunoblotting

• Molecular biology techniques – DNA & RNA extraction form animal tissue and cells, Q-PCR etc.

• Microbiology techniques – Phagocytosis assay, microbial cultures, colony forming units (CFU) enumeration etc.

Computer skills:-

1. Flow-cytometry data analysis using Fow-Jo and WIN-MDI software.

2. Statistical software – SPSS and Sigma Plot.

3. PC applications, MS office, MS DOS, Paint, Adobe Photoshop etc.

Employment History

King Saud Medical City Riyadh, Saudi Arabia

Senior Specialist, 17 July 2015 to till date

Performed and interpreted multiple real-time PCR assays in order to detect and characterize potential pathogens and ensure assay quality

Maintained chain of custody and sample integrity from DNA extraction through qPCR using aseptic BSL2 techniques.

RNA and DNA extractions for samples as well as inhibitor removal procedures

Maintained a clean and efficient laboratory setting, including calibration of instruments such as thermocyclers

Maintained scientific supply inventories via computer-based spreadsheets

Conducted supplemental scientific literature research and presented data

King Faisal Specialist Hospital and Research Centre Riyadh, Saudi Arabia

Medical Technologist, 02 February 2014 to April 2015

Working on automated urine analysis machines AX480 and IRIS 200 machines.

Maintain of daily and monthly QC records

Working on Vitek machine for microbiology analysis of bacteria and sensitivity

Extraction of RNA by using Abbot M2000 (sp) and M2000 (rt) real time PCR

Implementing and overseeing of testing programs to make sure all tests are performed properly

Working knowledge of Gene expert for clinical isolates

Isolation of Helicobacter growing microareophilic conditions and Cryptococcus species from CSF specimen using India ink

Maintains competency in computer functions in assigned areas.

Carrying out antigen and antibody reactions using ELISA for HIV, HBsAg, HCV, Toxoplasma

Detection of Corona virus by using Light Cycler PCR using EZ1 machine.

Detection of Epstein Barr Virus (EBV) by PCR using M2000 (sp)

Maintenance of trouble shooting machines.

Gulf Medical Laboratories, Diagnostic Center Saudi Arabia

Microbiologist, December 2012 – December 2013

Inoculation and reading of all types of culture e.g. Blood Culture, CSF culture Fungal culture, .API Index reading.

Perform all manual test like e.g. Helicobacter Pylori AB, C.Diff test, Faceal Occult Blood

Maintenance of required ATTC cultures types

Maintaining accurate log books of all performed procedures

Sampling and analysis of environment and water samples to determine presence of microorganisms

Knowledge of ELISA in HIV, HBsAg, HLTV-1 &2, TOXOPLASMA IgG, IgM, ANA

Dr Labs Laboratories,

Laboratory Supervisor July 2011 to August 2012 Hyderabad, India

Performing and supervising different microbiological test activities

Prepare documents for protocols

Identify the morphological and physiological characteristic of the micro organism

Maintenance of accurate records perform on each test

Maintaining the purchase orders of laboratory equipment

Serology using latex agglutination cards for RPR, H.Pylori, Infectious mono nucleosis along with titration

Carrying out urinalysis with Clinitek 250 for routine urine test and manually microscopy

Semen analysis with BD kit and calculating the sperm count

HCA (Hospital Corporation of America) Laboratories

Laboratory In charge Aug 2010 to Apr 2011 -London, U.K.

Hands on experience NASBA PCR for MRSA, knowledge of flow cytometry and sample preparation

Analysis of virus like RSV, Rota virus and Clostridium Difficille

Detection of Giardia,spp and cryptosporidium parvum in water samples

Validation of documentation, Physical and chemical properties involve in quality control microbiology

Handling of Analyzers, Centrifuges and PCR machines Like RT-PCR and proper maintenance

Familiar with HIV, STI and Viral Hepatitis strategies

Develop education program and provide necessary information for pre and post HIV

Coordinate with healthcare centers and identify HIV positive people

Test method validations, corrective and preventive action

Ability to work with VITEK for sensitivity of pathogen

Feeding of information using LIMS [Laboratory information and management system]

Reporting any interference in the implementation of infection control practices

Making ensure that the staff is free from any infectious disease

Maintenance of category room 3 [Inoculating Sputum’s, Stools]

UK Bio Bank -Microbiologist May 2009 to Aug 2010 -London, U.K.

Performed routine laboratory procedures such as preparation of media and basic preparation of slides and smears.

Carried out non-technical routine laboratory tests and procedures under the direction of professional staff.

Loading the blood cultures in BACTAC automated machine to process blood culture.

Performing microscopic examination of micro-organisms to determine their morphological and physiological characteristics

Setting up and operating laboratory equipment in preparation for specimen examinations.

Opens, separates, numbers and arranges specimens for laboratory examination and trace results.

Maintaining laboratory records and inventory for supplies and reagents.

Discarding of Media and Cultures and conduct sterilization of materials by Autoclaving and operating Dry Heat Sterilizer (DHS).

Preparation of Media for Primary, Secondary test and for Confirmation Tests.

Preparation of specification and testing procedure for finished product, Raw material and in-process sample for Microbial analysis.

Microbiological Monitoring of Environment in Sterile Production Area which includes Plate Exposure, Air Sampling, Contact Plate /

Swab Testing.

Preparing Trend Analysis Reports of Microbiological Monitoring and MLTs

Ibn Sina Hospital -Microbiologist May 2002 to Jul 2007 -Sirte, Libya

Staining of T.B samples

Training of new technicians.

To provide the proper guidance to the patients and all in order to avoid the infection in their place.

Preparation of reports including validation protocols and upgrading of documentation.

Complete isolation, identification and antibiotic susceptibility testing of significant bacteriological organisms.

Supervision of the activities, technicians and overall management of the hospital diagnostic laboratory.

Isolation and identification of pathogenic bacteria, fungi and parasites in water and blood samples.

Carried out serological tests using ELISA.

Carried out finger stick testing for detecting HIV antibodies

Uniqare Hospital -Microbiologist Mar 2000 to Jan 2002- Hyderabad, India

Conduct periodic micro surveillance of facilities. Locate and solve contamination issues when necessary.

Staining and culturing of TB sputum samples in culture media.

Responsible for the efficient operation of micro laboratory and maintenance of all lab equipment.

Overlook the isolation and purification of human pathogenic bacteria from hospital samples for onward referral to a pharmaceutical company.

Sanitation of Sterility & Microbiological Department with different disinfectant at regular intervals.

Validation of LAF, Autoclave, Incubator as per SOP

Railway Hospital -Trainee Microbiologist Jun 1997 to Dec 1999 - Hyderabad, India

Assisting in all sections of the diagnostic microbiology laboratory of a tertiary-level hospital.

Good working knowledge of methods and procedures used through Quality Systems are fundamental to Laboratory operations.

Overlook the isolation and purification of human pathogenic bacteria from hospital samples for onward referral to a pharmaceutical company and responsible for the efficient operation of the micro laboratory and maintenance of all lab equipment.

Personnel Information

Name [as per passport] : MOHAMMED KARIMULLAH MULTANI PASHA

Gender : Male

Nationality [Present& Past]: Indian

Marital Status & No of

Dependents : Married, Wife & Two children

Residential Address : Plot # 52 H.No. 1-9-485/13/1

Lalithanagar colony, Jamia Osmania

Adikmet, Hyderabad 500 044

Andhra Pradesh, India

PH: +91-402-***-****

MO: 009***********

Email: rhupfz@r.postjobfree.com

Date of Birth : 09/04/1976’ Age # 40

Languages : English, Hindi, Telugu, Urdu, Arabic

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