BIOGRAPHICAL SKETCH

NAME

Yin-Xiong Li, M.D., Ph.D. POSITION TITLE

Assistant Professor

eRA COMMONS USER NAME

li000031

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE

(if applicable) YEAR(s) FIELD OF STUDY

Hunan Medical University (Established by Yale-China Association at Hunan in 1914) M.D. 1979-1985 Medicine

Peking Union Medical College (Established by Rockefeller Foundation at Peking in 1917) Ph.D. 1987-1991 Molecular Biology

A. Positions and Honors

Positions and Employment

1985-1987 Research Associate, Chinese Academy of Medicines Sciences (CAMS) and Peking Union Medical College (PUMC)

1991-1992 Assistant Professor in China at CAMS and PUMC Visiting Assistant Professor at China/Japan Friendship Hospital, Clinic Research Institute

1992-1994 Research Scientist, Vanderbilt University Medical Center

1993-2001 Research Scientist, Medical College of Georgia

2001-2004 Assistant Professor, Dept. of Pediatrics and Cell Biology, Duke University Medical Center

2004-present Assistant Professor, Dept. of Medicine (Gastroenterology and Cardiology), Pediatrics and Cell Biology, Duke University Medical Center

Other Experience and Professional Memberships

2005-2008 American Heart Association, Study Section, Panel of Cellular and Molecular Biology

2009 NIH Study Section, Challenge Grant Editorial Panel 18, Center for Scientific Review Special Emphasis Panel (ZRG1 DKUS A58, Liver stem cell study)

2010 NIH Study Section, NIAAA Special Emphasis Panel (ZAA1, DD 01, Alcohol center grant)

Honors

1985 Highest Honors, Outstanding M.D. Graduated Student Hunan Medical University

1987 Award of Outstanding Young Scientist, Issued by Chinese Academy of Medical Sciences

1991 International Young Scientist Travel Award in June. Issued by the United Nations of Education, Science and Culture Organization (UNESCO).

1992 Chief of the National Molecular Biological Techniques Training Center

1992 Award for “Outstanding Correspondent” of the J Chinese Academy of Medical Sciences issued by the Chinese Academy of Medical Sciences

1992-1994 NIH Fogarty International Young Scientist Fellowship.

1996 National Science & Technology Progress Prize issued by: National Health Ministry, People’s Republic of China

1996 National Excellence Educational Publishing Prize issued by: National Education Ministry, National News and Publication Agency, People’s Republic of China

1999 Designated as an “Alien with Extraordinary Ability in Science and Education” by the US Immigration and Naturalization Service

2006 Duke Stanback Award for environmental factors, stem cell and tumorgenesis research.

Books: Li Yin-Xiong, et al. Editor, Modern Experimental Technology of Molecular Biology. (A textbook for undergraduate and graduate in China). National College Publishing, Inc. Beijing, China (1993). Second Edition (1995), Third Edition (2000).

Gene Accession Number AF093110

Li Yin-Xiong , who firstly discovered the cell death inhibitor gene DAP5. This gene is specific expressed in premigratory cardiac neural crest which support to the heart development. The Gene accession number for its publication in NIH Gene Bank is AF093110 that is under Dr. Li’s name.

Membership

Chinese Oncological Society

Chinese Biochemical Society

Chinese Association of Medicine

American Society for Cell Biology

Society for Developmental Biology of American

Federation of American Societies for Experimental Biology

The American Association for the Advancement of Science

Patents

1. Li Y-X. Nucleic Acid Filter for Tumorigenic Factors. USA Patent Number: 6,117,846, Approved and published date: September 16, 2000

2. Li Y-X, Farrell M, Kirby ML. Double stranded RNA blocks specific gene expression in muticellular settings in vivo and in vitro. USA patent Application No. 200********, 2002

3. Li Y-X and Kirby ML. RNA profiling of laser capture single or multiple cells. USA. Application No. 200********, 2005. International application No.: PCT/US03/32115, 2003

4. Li Y-X Enhanced broad-spectrum UV radiation filters and methods. Application No. 200********, 2008

5. Li YX. Formation and Rejuvenation of Organs and Alcohol Damaged Organ Regeneration through Stem Cell Nutrients. USA. Application No. 200********, 2009

B. Selected peer-reviewed publications (in chronological order)

1. Li Y-X, Li, SO. DNA methylation and gene regulation research of oncogene c-myc and differentiation enzyme genes CPS1. Paper Collection of Young Scientist of Chinese Academy of Medical Sciences 2:211-217, 1988.

2. Li Y-X. Cancer cells possess the potential for speciation. J Exp Clin Immunol 2:46-47, 1990.

3. Hong ZT, Li Y-X. Effects of c-myc antisense RNA on cell growth and biological macromolecular biosynthesis of NIH/3T3 cells and gastric cancer cell lines. J China-Japan Friendship Hospital. 6:235-238, 1992.

4. Robinson-Benion C, Li Y-X, Holt JT. Gene transplantation: combined antisense inhibition and gene replacement strategies. Leukemia. 8:152-155, 1994. PMID: 8152283

5. Li Y-X, Fan M, Zhang J and Liang ZQ. Expression of antisense constructor reverses the tumorgenesis phenotype in HL60 cell line. Chinese Journal of Cancer Biotherapy. 1995, 2(1) 34-38.

6. Li Y-X, Fan M, Zhang J and Liang ZQ. Expression of c-myc gene and biosynthesis of biological macromolecules in antisense transfectant HLR60-9. Chinese National Journal of Cancer research (Chung Hua Chung Liu Tsa Chih) 18:16-19, 1996.

7. Li Y-X, Papkoff J, Sarkar NH. Antisense downregulation of a mouse mammary tumor virus activated protooncogene in mouse mammary tumor cells reverses the malignant phenotype. Virology 255:138-149, 1999. PMID: 10049829

8. Farrell M, Waldo K, Li Y-X, Kirby ML. A novel role for cardiac neural crest in heart development. Trends Cardiovasc Med 9:214-20, 1999. PMID: 10881754

9. Li Y-X, Farrell MJ, Liu RP, Mohanty N, Kirby ML. Double-stranded RNA injection produces null phenotypes in zebrafish. Development Biology. 217:394-405, 2000. PMID: 10625563

10. Chatterjee B., Li Y-X, Zdanowicz M., Sonntag J. M., Chin A. J., Kozlowski D. J., Valdimarsson G., Kirby M.L. and Lo C.W. Analysis of Cx43α1 promoter function in the developing zebrafish embryo. Cell Communication and Adhesion 8:289-92, 2001. PMID: 12064604

11. Kirby ML, Lawson A, Stadt HA, Kumiski DH, Wallis KT, McCraney E, Waldo KL, Li Y-X and Gary C. Schoenwolf. Hensen's node gives rise to the ventral midline of the foregut: implications for organizing head and heart development. Developmental Biology 253:175-188, 2003. PMID: 12645923

12. Li Y-X, Zdanowicz M, Young L, Kumiski D, Leatherbury L and Kirby ML. Cardiac neural crest in zebrafish embryos contributes to myocardial cell lineage and early heart function Development Dynamics. 226:540-550, 2003. PMID: 12619138

13. Li Y-X, Kirby ML. Coordinated and conserved expression of alphoid repeat and alphoid repeat-tagged coding sequences. Developmental Dynamics 228(1):72-81, 2003. PMID: 12619138

14. Wilkbanks AM, Fralish GB, Kirby ML, Barak LS, Li Y-X* and Caron MG.* Arrestin 2 regulates zebrafish development through the sonic hedgehog pathway. Science. 306:2264-2267, 2004. (*co-correspondence authors). PMID: 15618520

15. Sicklick J, Li Y-X, Choi S, Qi Y, Chen W, Bustamante M, Huang J, Zdanowicz M, Camp T, Torbenson MS, Rojkind M and Diehl AM. Role for Hedgehog signaling in hepatic stellate cell activation and viability. Lab Investigation. 85:1368-80, 2005. PMID: 16170335

16. Sicklick JK, Li YX, Jayaraman A, Kannangai R, Qi Y, Vivekanandan P, Ludlow JW, Owzar K, Chen W, Torbenson MS, Diehl AM. Dysregulation of the Hedgehog pathway in human hepatocarcinogenesis. Carcinogenesis. 2005 Dec 8; [Epub ahead of print], 27:748-57, 2006. PMID: 1633918

17. Sicklick JK, Li YX, Melhem A, Schmelzer E, Zdanowicz M, Huang J, Caballero M, Fair JH, Ludlow JW, McClelland RE, Reid LM, Diehl AM. Hedgehog signaling maintains resident hepatic progenitors throughout life. Am J Physiol Gastrointest Liver Physiol. 27:748-57, 2006. PMID: 16322088

18. Hutson ML, Zhang P, Stadt HA, Sato A, Li YX, Burch J, Creazzo TL, Kirby ML, Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx. Developmental Biology, 295(2):486-97. 2006. PMID: 16765936

19. Sicklick JK, Choi SS, Bustamante M McCall SJ, Hernandez-Paz S, Huang J, Li YX, Rojkind M and Diehl AM. Evidence for Epithelial-mesenchymal transitions in adult liver cells. Am J Physiol Gastrointest Liver Physiol. 291(4):G575-83. 2006. PMID: 16710052.

20. Suzuki A, McCall S, Choi SS, Sicklick JK, Huang J, Qi Y, Zdanowicz M, Camp T, Li YX and Diehl AM. Interleukin-15 increases hepatic regenerative activity. J Hepatol. 45(3):410-8. 2006. PMID: 16781000.

21. Choi SS, Sicklick JK, Ma Q, Yang L, Huang J, Qi Y, Chen W, Li YX, Goldschmidt-Clermont PJ and Diehl AM. Sustained activation of Rac1 in hepatic stellate cells promotes liver injury and fibrosis in mice. Hepatology. 44(5):1267-77, 2006. PMID: 1705826.

22. Li YX*, Yang HT, Danowicz M, Sicklick JK, Qi Y, Camp T and Diehl AM. Fetal Alcohol Exposure Impairs Hedgehog Cholesterol Modification and Signaling. Lab Invest. 87(3):231-40. 2007. (Presented as the Journal cover picture and with an Editorial Press Release) (* correspondence authors). PMID: 17237799.

23. Omenetti A*, Yang L*, Li YX, McCall SJ, Sicklick JK, Huang J, Choi S, Suzuki A and Diehl AM. Hedgehog mediated mesenchymal-epithelial interactions modulate hepatic response to bile duct ligation.. Lab Invest. 87(5):499-514. 2007. (* Equally contribution). PMID: 17334411.

24. Jung Y, McCall SJ, Li YX and Diehl AM. Bile Ductules and Stromal Cells Express Hedgehog Ligands and/or Hedgehog Target Genes in Primary Biliary Cirrhosis. Hepatology. 45(5):1091-6. 2007. PMID: 17464985

25. Yamaguchi K, Yang L, McCall S, Huang J, Yu XX, Pandey SK, Bhanot S, Monia BP, Li YX, Diehl AM. Inhibiting triglyceride synthesis improves hepatic steatosis but exacerbates liver damage and fibrosis in obese mice with nonalcoholic steatohepatitis. Hepatology. 45(6):1343-6. 2007. PMID: 17476695.

26. Yamaguchi K, Yang L, McCall S, Huang J, Yu XX, Pandey SK, Bhanot S, Monia BP, Li YX, Diehl AM. Diacylglycerol acyltranferase anti-sense oligonucleotides reduce hepatic fibrosis in mice with nonalcoholic steatohepatitis. Hepatology 47:625-35. 2008. PMID: 18000880.

27. Yang L, Wang Y, Mao H, Fleig S, Omenetti A, Brown KD, Sicklick JK, Li YX, Diehl AM. Sonic hedgehog is an autocrine viability factor for myofibroblastic hepatic stellate cells. Journal of Hepatology 48:98-106. 2008. PMID: 1802272. PMC2196213.

28. Hua Mao H., Diehl AM., Li YX. Sonic Hedgehog Ligand Partners with Caveolin-1 for Intracellular Transportation. Lab Invest. 89:290-300. 2009. PMID: 19139721. PMC2647995

Selected abstracts

1. Holt, J. T. and Li Yin-Xiong, Antisense strategy for gene transplantation: combined gene inhibition and gene replacement. Abs. in Antisense and Gene Therapy. (1993).

2. Li Yin-Xiong, and Sarkar N. Antisense-mediated in Wnt-1 expression retards the growth of a transplattable mouse mammary tumor cell line. Proceeding of the American Association for Cancer Research. 37:506a. (1996).

3. Li, Y.X. & Kirby ML. Differential expression of alphoid repetitive sequence in embryogenesis. Molecular Biology of the Cell 7s:486a. (1996).

4. Kirby, M. L., Wallis, K. T., Rhodes, M. R., Stadt, H. A., Kumiski, D. K., Waldo, K.L. and Li, Y.X. A novel serine protease associated with premigratory neural crest. Molecular Biology of the Cell. 7s:486a. (1996).

5. Li, Y.X. & Kirby ML. Differential expression of alphoid repetitive sequence in embryogenesis. Molecular Biology of the Cell 7s:486a. (1996).

6. Kirby, M. L., Wallis, K. T., Rhodes, M. R., Stadt, H. A., Kumiski, D. K., Waldo, K.L. and Li, Y.X. A novel serine protease associated with premigratory neural crest. Molecular Biology of the Cell. 7s:486a. (1996).

7. Li, Y. X., Kumiski, D. and Kirby M. L. Alpha repeat sequence tags may coordinate expression of certain cranial and cardiac genes. Molecular Biology of the Cell. 8s:212a (1997).

8. Li, Y. X., Kumiski, D. and Kirby M. L. Alpha repeat sequence tags may coordinate expression of certain cranial and cardiac genes. Molecular Biology of the Cell. 8s:212a (1997).

9. Li, Y. -X., Kumiski, D. Young, L., Leatherbury, L. and Kirby M. L. Cardiac neural crest ablation in zebrafish embryo. Abstract of Weinstein Cardiovascular Development Conference P52. (2000)

10. Silva, JE., Li, YX. And Creazzo, TL. Ca++ channel B subunit expression in early heart tube following ablation of the cardiac neural crest. Journal of Molecular and Cellular Cardiology 32(5):A27. (2000)

11. Li Y. -X, Zdanowicz M, Lo CW and Kirby ML. Dissection of connecxin 43 promoter regulatory elements of tissue specificity. Abstract of Weinstein Cardiovascular Development Conference P29. (2001)

12. Yin-Xiong Li and Margaret L. Kirby. Laser microdissection captured cells gene expression profile analysis hunting down ventral axis molecular players in vertebrates involved in three human genetic syndromes. Oral presentation in functional Genomics section in The Seventh International Human Genome Meeting organized by The Human Genome Organization (HUGO) in Shanghai.(April, 2002)

13. Li Y-X., M. Zdanowicz, H. Stadt and M. Kirby. The ventral midline endoderm constitutes a molecularly distinct population of cells. 61st Development Biology Annual Meeting abstract 368. (2002). Oral presentation.

14. Li Y-X., and Kirby ML. Laser microdissection captured cells gene expression profile analysis hunting down ventral axis molecular players in vertebrates involved in three human genetic syndromes. Functional Genomics section in The Seventh International Human Genome Meeting organized by The Human Genome Organization (HUGO). (2002). Oral presentation.

15. Li Y-X and Kirby ML. Optimization of the conditions for micro-dissected single or multiple cells for gene profiling analysis. Symposium on phenotyping mouse cardiovascular function and development. NHLBI/NIH (2002). Dr. Kirby Oral Presentation.

16. Millington T., Zdanowicz M., Zhang P., Kirby ML. and Li Y-X. FGF8-dependent expression of erm and pea3 contributes to neural crest supporting the development of the caudal pharynx and heart. Weinstein Cardiovascular Development Conference Boston (2003). Abstract 153

17. Abu-Issa R., Li Y-X., Kirby ML., Brown D., Martin G. and Meyers E. Fgf8 is required for outflow tract lengthening and development. National Meeting of American Heart Association in sunny Orlando, Florida, (2003). Dr. Abu-Issa Oral Presentation.

18. Margaret L. Kirby, Mary R. Hutson, Cary C. Ward, Y-X Li. Collaboration of Cardiac Neural Crest and Caudal Pharynx Is Needed for Patterning the Preseptation Arterial Pole. Keystone Symposia of Cardiac Development and Congenital Heart Disease, Keystone Resort, Keystone, Colorado. March 7-12, 2004.

19. Sato A, Li Y-X, Zhang P and Kirby ML. Analysis of Fgf8 signaling modulated by cardiac neural crest. The American Society for Cell Biology 44th Annual Meeting. Washington, DC (2004).

20. Sackey FN, Barbokey LA, Zhang P, Stadt HA, Zdanowicz M, Li Y-X and Kirby ML. Sonic hedgehog signaling is upregulated in DiGeorge syndrome model o fchichk embryonic cardiac neural crest-ablation. The American Society for Cell Biology 44th Annual Meeting. Washington, DC (2004).

21. Grimes AC, Zdanowicz M, Li Y-X and Kirby ML. Striated to smooth muscle transition in the developing. zebrafish cardiac outflow tract. The American Society for Cell Biology 44th Annual Meeting. Washington, DC (2004).

22. Meyers E, Abu-Issa R, Illagen R, Kirby M, Brown D, Li YX, Schwartz R. Fgf8 is critical for anterior heart field development. Developmental Biology 2004;271:622-622.

23. Zhang HT, Parker J, Li YX, VanDongen AMJ, Creazzo TL. Function of a two pore domain potassium channel (TASK-1) in developing chick heart. Biophysical Journal 2004;86:550A-550A.

24. Sicklick JK, Li YX, Melhem A, Schmelzer E, Zdanowicz M, Huang JW, Caballero M, Fair JH, Ludlow JW, Reid LM, Diehl AM. Conserved hedgehog pathway regulation of murine and human hepatic progenitors throughout life. Hepatology 2005;42:740A-740A.

25. Sicklick JK, Rojkind M, Qi Y, Chen W, Bustamante M, Choi SS, Huang JW, Camp T, Torbenson MS, Li YX, Diehl AM. Hedgehog signaling in hepatic stellate cells from adult mice and rats. Hepatology 2005;42:733A-733A.

26. Suzuki A, McCall S, Huang JW, Qi Y, Zdanowicz M, Sicklick J, Camp T, Li YX, Diehl AME. IL-15 increases regenerative activity in the livers. Hepatology 2005;42:572A-572A.

27. Zdanowicz M, Sicklick JK, Diehl AM, Li YX. A zebrafish model of Fetal Alcohol Syndrome parallels Hedgehog signaling defects. Alcoholism-Clinical and Experimental Research 2005;29:86A-86A.

28. Omenetti A, Li YX, Chen W, Gainetdinov RR, Yang L, Diehl AM. Cross-talk between hepatic stellate cells and cholangiocytes regulates biliary growth through serotonin. Hepatology 2006;44:392A-393A.

29. Omenetti A, Li YX, Yang L, Sicklick JK, Choi S, Suzuki A, Diehl AM. Mesenchymal-epithelial signalling by the Hedgehog pathway promotes proliferation of bile ductular cells. Hepatology 2006;44:389A-389A.

30. Yamaguchi K, Yang L, McCall S, Huang JW, Yu XX, Pandey SK, Bhanot S, Monia BP, Li YX, Diehl AM. DGAT1 ASO treatment reduces hepatic fibrosis without improving hepatic steatosis in DB/DB mice fed methionine choline deficient diets. Hepatology 2006;44:663A-663A.

31. Yamaguchi K, Yang L, McCall S, Huang JW, Yu XX, Pandey SK, Hanot S, Monia BP, Li YX, Diehl AM. DGAT2 aso treatment improves hepatic steatosis, but not fibrosis in DB/DB mice fed methionine choline deficient diets. Hepatology 2006;44:665A-665A.

32. Yamaguchi K, Yuen BB, Camp T, Zdanowicz M, Sicklick J, Hinton D, Diehl AM, Li YX. A novel model for harvesting adult liver progenitor cells. Hepatology 2006;44:473A-473A.

33. Yang L, Wang Y, Mao H, Fleig S, Omenetti A, Brown K, Sicklick JK, Li YX, Diehl AM. Sonic hedgehog is an autocrine viability factor for myofibroblastic hepatic stellate cells. Hepatology 2007;46:866A-867A.

34. Hua Mao H., Diehl AM., Li YX. Sonic Hedgehog Ligand Partners with Caveolin-1 for Intracellular Transportation. The American Society of Cell Biology, Annual meeting in San Francisco, Molecular Biology of the Cell 2008:L169.

C. Research Projects Ongoing or Completed

AA016001-01 (Principal Investigator, Li), 11/01/07-11/30/10

NIAAA/NIH

Cholesterol, Hedgehog Signaling & Fetal Alcohol Syndrome The aim of this project is to determine whether one teratogenic mechanism of Fetal Alcohol Syndrome is defective Hh signaling via alteration in cholesterol modification of a morphogen, Sonic Hedgehog.

AA016144-01 (Principal Investigator, Li), 06/01/06-05/30/09

NIAAA/NIH

Metabolic Profiling to Identify Alcohol Biomarker Signature

The aim of this project is to determine whether alcohol disturbs the homeostasis of lipid metabolism and generates a molecular-metabolic fingerprint.

Stanback Award (Principal Investigator, Li) 12/01/05-12/31/07

Duke DCCC/NSOE

Environmental regulation of liver progenitors in zebrafish model of hepatocarcinogenesis

To develop a progenitor cell model of HCC in zebrafish and to manipulate the activities of signaling pathways that promotes progenitor cell viability and proliferation and assess the impact on hepatocarcinogenesis.

AA010154 (Diehl), 08/01/97-07/31/09

NIAAA/NIH

Role of TNF Alpha in Recovery from Alcoholic Liver Injury

The aim of this project is to determine mechanisms that regulate TNF alpha induction and the hepatic responses to this cytokine during liver regeneration in normal and alcohol-fed mice. Role on project: design oversight and data analysis of experiments involving molecular biology.

AHA 0430250N (Principal Investigator, Li), 07/01/04-06/30/07

AHA

Characterization of FGF8 regulated Pea3 subfamily function in craniofacial and cardiovascular development

The aim of this project is to study the pea3 subfamily function that plays a critical role to mediate FGF8-dependent neural crest differentiation in cardiovascular and craniofacial development in zebrafish.

HD17063 (Kirby) 04/01/97-03/31/03

NIH/NICHD Yin-Xiong Li, Co-Investigator

Autonomic Interactions in Developing Heart

The aim of this project is to determine the role of Notch family genes in differentiation of cardiac neural crest cells. I developed a method for RNAi in vivo and in vitro. Patent Pending Number: 13027.0014 USA. This patent describes a novel method for attenuating the expression of targeted genes in tissue explants and in multicellular vertebrate embryos using double-stranded RNA.

HL 36059 (Kirby), 04/01/00-03/31/05

NIH/NHLBI

Development of the Heart: Role of Neural Crest

Project 1: Characterization and modification of cardiac neural crest cells. Kirby (PI), Li (Co-PI)

The aim of this project is to explore the role of cardiac neural crest in modulation of FGF family expression for normal myocardial maturation. I discovered the cell death inhibitor gene DAP5. This gene is specifically expressed in pre-migratory cardiac neural crest, which supports to the heart development (NIH Gene Database accession Number: AF093110). More recently I successfully developed the application of Laser Capture Microdissection for isolating few targeted embryonic cells from which RNA is isolated. I demonstrated that RNA from few cells can be successfully amplified and applied on DNA microarray for gene profile analysis. This provides the potential to establish a precise gene expression profile for any cell or tissue during development and differentiation.

HL 39946 (Gourdie) 05/01/02-04/30/06

Patterning by Invasive Mesenchyme in the Embryonic Heart

Project 3 “Cx43 Gap Junctions in Heart Development and the Role of Extracardiac Cells”

Yin-Xiong Li, Co-Investigator for Project 3

The aims of this project are to characterize Cx43 expression in the secondary heart field and the role of wnts in Cx43 expression and myocardial differentiation. I did search the upstream regions and found the enhancers of Cx43 gene response to the expression in neural crest and heart. Transgenic zebrafish line has been established and the targeted tissues expression pattern is confirmed.

HL070140 (Kirby) 04/01/03-03/31/07

“Characterization of Secondary Heart Field”

The goals of this project are twofold: to determine whether the secondary heart field has an origin distinct from the primary heart fields; and to determine whether commitment of the myocardial stem cell population in the secondary heart field is effected by fibroblast growth factor and bone morphogenetic protein signaling pathways.

Supervisor and Mentorship for Ph. D and MD. Students

Tim Milington, MD student research program, 2002-2004.

Alyson MacInnes Wilbanks, Cell Biology Ph.D. program, 2002-2005

Liberty Hodge, Developmental Biology Ph.D. Program, 2003-2004.

Sajid Anwer, Undergraduate student research program, 2003-2005

Hao Tang, Cell Biology Ph.D. Program, 2002-2006

Hai Tao, Ph.D. NIH R32 postdoctoral research fellow, 2004-2006

Megan Diehl, MD. Student Research, 2006

Guanyu Xu, Undergraduate student research program, 2006-2008

Gurram Ananda R., MD. student Research 2007-2009

Sean Ang Lee, Undergraduate student research program, 2009-2010

Mao Hua, Ph. D. Postdoctoral Fellow, 2006-2009

Xia Qing, MD., Ph. D., Postdoctoral Fellow, 2006-2009

Original Scientific Contributions of Major Significance in the Field

1. First person who discovered the cell death inhibitor gene DAP5. This gene is specific expressed in premigratory cardiac neural crest stem cells which support heart and craniofacial development. NIH Gene Database accession Number: AF093110.

2. Developed a induced promoter DNA construct making antisense RNA targeted to wnt-1 oncogene which significantly block the mammary tumor cell growth in mice. This work was praised by Nature Biotechnology in a review on gene therapy for cancer. (Nature Biotechnology April 1999, Volume 17, Number 4, Page 403-404.) In this international highly prestigious journal, Dr. Aris Persidis, the vice president for business development at Argonex Inc., wrote a review on antisense therapeutics research. In this review, the history and current state of antisense therapeutics programs to treat diseases ranging from cancer to AIDS were discussed. Dr. Persidis referred to Dr. Yin-Xiong Li’s work as a significant step toward the application of antisense treatment for virally induced cancer. The work referred to was published in Virology March, 1999, Volume 255, Page 138-149.

3. USA patent: Li Yin-Xiong, Nucleic Acid Filter For Tumorigenic Factors. USA Pending Number: 08939,858 USA. 2000. This patent describes a number of materials and methods to prevent the deleterious effects of DNA damaging agents, which are potent carcinogens. In particular, it presents a revolutionary breakthrough for sunscreen protection. This new sunscreen contains a component which filters out DNA damaging UV light much more efficiently than any previously described product. It also establishes an important new concept and standard for determining UV light induced DNA damage, the GPF (Gene Protection Factor). GPF more accurately reflects the protection efficiency of the sunscreen than does the classical SPF standard (Sunlight Protection Factor), which is determined by how well the sunscreen prevents the change in skin redness induced by UV light.

4. USA patent application: Li Yin-Xiong, Farrell, Michael and Margaret L. Kirby (1999). Double stranded blocks specific gene expression in muticellular settings in vivo and in vitro. This patent describes a novel method for attenuating the expression of targeted genes in tissue explants and in multicellular vertebrate embryos using double-stranded RNA. The ability to attenuate the expression of targeted genes in multicellular vertebrate tissues has broad implications for future medical applications. These may include anti-viral agents, anti-tumor agents, and other therapeutics that are designed to block the expression of specific genes or specific gene alleles, such as the autosomal dominant allele that causes Huntington’s chorea.

5. “Modern Experimental Technology of Molecular Biology”, 1993. Second Edition (1995), Third Edition (2000). National College Publishing Inc., Beijing, China. National Publishing Book Number: ISBN 7-04-004571-0/Q.215. This is a textbook for undergraduate and graduate students in Molecular Biology. Two editions of this book have been published. This textbook has been used at more than 200 Chinese universities including medical colleges, agricultural colleges and in Biology programs at universities.

6. Proposed new standard for cancer cell classification based on molecular oncogenesis pathology. This work was published in Journal of Experimental and Clinical Immunology, 2: 46-49, 1990. Two of the most popular media outlets in China have covered this story. The China Central TV station broadcast a story about this work at 9:00 PM on November 15, 1989. The Peking People’s Radio Station broadcast a story about this work at 3:00 PM on November 15, 1989. News programs at both stations reported that the work of Dr. Li Yin-Xiong and Dr. Shi-Dong Liao on a new molecular pathological classification of cancer cells, which made a significant step toward improving diagnosis and treatment of various tumors.

7. Successfully developed a RNA amplification approach to amplify RNA from Laser Capture. Microdissected few targeted cells and apply to oligonucleitids microarray research. This provides a great potential to establish precise gene expression profile for any cell or tissue which involved interesting biological event or disease. 2001.

8. Invented a way to isolate and purify adult liver stem cells, and ten of these cells can rescue on damaged liver regeneration, 2005

9. The molecular signaling defects result in stem cell developmental abnormalities, 2007. Discovered that alcohol induced birth defects are rooted in small molecular alternation of cholesterol modification of a key protein, Shh. A breakthrough of molecular mechanism of alcohol-induced birth defects was published online in Jan 22, 2007 with a Press Release on Naure.com. In the first week after online publish, journalists from top five scientific magazines and over hundred websites reported my finding. These journals are: Science Dairly, Nature.com, New Scientist (USA), Chemistry and Industry (England), Theheart.com (England), Daily Telegraph, (London). Newsweek, (Russia). This discovery has a profound impact on prevention and diagnosis of alcohol induced birth defects and adult tissue damages.

10. Discovered cell signaling molecules, 2009. Three very important natural components have been found that function as nutrients for Adult Stem Cell; five components have been identified as signaling molecular among gastroenterological track and boy tissues to regulate food intake and energy homeostasis. It holds a great potential for contorting obesity, hypotension and diabetes. Dr. Li’s new concept “Stem Cell Nutrient” and related technology is monumental for leading adult stem cell based health care and clinical practice in the coming years. This technology will have a major impact in anti-aging, organ regeneration, and prevention of alcohol related diseases.

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