Resume
Resume:
Dr. Anna Reeves
Northumberland, PA 17857
H: 310-***-****
W: 570-***-****
****.******@*****.***
http://www.biologyofcancer.org/Anna.html
Objective
A responsible leadership position based on my experience in both research and teaching.
Experience
International Scientific Director and Board Member
NIET (North Indian Educational Trust) Uttar Pradesh, India Nov '08 - Present
Establishing the Laboratory for Molecular Diagnostics of Cancer in Women and Children in Northern India; obtaining Indian government grants to fund the activities of the NIET. This laboratory will be established as a charity to benefit women and children in the rural northern Indian state of Uttar Pradesh.
Research Scientist I
Geisinger Clinic Danville, PA Aug '04 - Oct '08
Planning and conducting biomedical cancer-related research, teaching in the undergraduate college and residents, planning and analyzing experiments, writing grants and scientific papers.
Senior Research Associate
USUHS Bethesda, MD Sep '99 - Aug '04
Project 1: Transcriptional regulation of human glucocorticoid receptor gene (hGR) expression is accomplished by alternative promoter utilization.
5'-heterogeneity in the hGR mRNA was identified by cDNA library screening, and the physical organization of the promoter regions has been determined using a genomic BAC clone. Luciferase reporter constructs containing each of the identified promoter regions have been tested for promoter activity in transient transfected tumor cell lines. The functional analysis of each of the major 5'-mRNA isoform has been investigated by using siRNAs specifically targeting the individual isoforms.
Project 2 (in collaboration with the Department of Biochemistry, USUHS): Localization, topology and function of the LCB1 subunit of Serine Palmitoyl Transferase (SPT)-a key enzyme in sphingolipid metabolism. The mutations in the LCB1 subunit of SPT cause hereditary sensory neuropathy. The RNA interference (RNAi) approach has been employed for the analysis of the lcb1 gene function in transiently and stably transfected cultured cells.
Postdoctoral Fellow
American Red Cross Rockville, MD Feb '98 - Sep '99
Project: Biotech industry-sponsored construction of transgenic vectors for targeted expression of fibrinogen blood proteins in mouse and pig mammary glands. Work involved multistep cloning of fibrinogen alpha-, beta-, and gamma- into pKUN vector under a-S1-casein promoter; zygosity and expression testing (Southern-, slot-blotting, Northern-blotting, RT-PCR, S1 nuclease protection mapping), inbreeding and interbreeding of transgenics, postmortem identification of pathologies.
Postdoctoral Fellow
USUHS Bethesda, MD Feb '97 - Feb '98
Project: Regulation of cell cycle in tumor cell lines, in particular, non-S-phase tumor cell sensitivity to anti-tumor DNA topoisomerase 1 (Top1) -targeting drugs. Demonstrated that replication-independent cytotoxicity of Top1 inhibitor camptothecin could be a potential determinant of time/course drug treatment in the clinical setting.
Postdoctoral Fellow
NIH Bethesda, MD Mar '92 - Feb '97
Project: Isolation and characterization of IA-2 and IA-2 beta genes- major autoantigens in IDDM (insulin-dependent diabetes mellitus).
A radioimmunoprecipitation assay was developed to measure antibodies recognizing IA-2 and IA-2â in the sera of patients with IDDM. The presence of these antibodies in the human sera was shown to be highly predictive in identifying individuals at high risk for IDDM.
Project: Molecular mechanisms of radiation-induced apoptosis in fetal rat brain. Microscopic examination of histological sections with conventional and fluorescent stains revealed an extensive cell death morphologically consistent with apoptosis, accompanied by genomic DNA fragmentation and changes in levels of mRNAs relevant to apoptosis. Subtractive hybridization identified several other modestly induced transcripts.
Education
Doctor of Philosophy (Radiobiology and Molecular Biology)
Research Institute for Roentgenology and Radiobiology St. Petersburg, Russian Federation Jul '91
Master of Science, Bachelor of Science (Biology and Biochemistry)
Leningrad State University St. Petersburg, Russian Federation Jul '78
LANGUAGES: English, Russian
PERSONAL: Maiden name - Borovitskaya
SOCIETIES: American Association for Cancer Research
European Association for Cancer Research
Radiation Research Society
Russian Biochemical Society
Russian Radiobiological Society
AWARDS: 2006 - Concept Award for the proposal "Curcumin-Combretastatin Nanocells as Breast Cancer Cytotoxic and Antiangiogenic Agent" from the Department of Defense Breast Cancer Research Program (BCRP) of the Office of the Congressionally Directed Medical Research Programs (CDMRP).
2007 - Travel award for the presentation entitled "Role of the WW binding motif of the EGR-1 in its binding and transactivation function" at the 13th International Congress of Radiation Research, San Francisco, July 8-12, 2007.
COURSES AND WORKSHOPS: 1. Bioinformatics Tools for Comparative Genomics - Workshop, July 15-19, 2002, Berkeley, CA;
2. A Field Guide to GenBank and NCBI Resources, NCBI, March 27, 2003, Bethesda, MD.
3. Nanotechnology workshop, NCI, July 13, 2006, Frederick, MD
PROFESSIONAL SKILLS:
Technical:
- Molecular Biological Techniques: all basic and contemporary molecular and cellular biology techniques, generation of transgenic vectors, generation of nanovectors for anticancer drugs;
- Histology: sectioning of embedded and frozen tissues for light microscopy, immunohistochemistry and in situ- hybridization;
- Other: tissue culture, transient and stable transfection of the cultured cells, animal husbandry and surgery
Informatics:
Variety of computer software programs for genomic analyses, gene and protein sequence design, data analyses and presentation including Web-based programs, etc.
Managerial:
Supervised a project team at the RIRR; supervised the research of summer students and undergraduate students at NIH;
Put together and taught the course "Mammalian Radiation Biology" for the Residents of Radiation Oncology at the Fox Chase Cancer Center;
As an Adjunct Professor at the Department of Biology of the Susquehanna University currently teach the lecture course and the Lab "Issues in Human Biology" for the students in the Continuing Education Program, and the Organismal Biology Laboratory for the undergraduate students.
Publications and Presentations
SCIENTIFIC PUBLICATIONS
1. Flerov M.A., Tolstuhina T.I., Borovitskaya A.E., The influence of picrotoxin on the ATPase activity of subcellular fractions from rat brain cortex. In: Nervous System (ed. Leningrad University), 1980:20, 13-17.
2. Borovitskaya A.E., Tolstuhina T.I., Flerov M.A. The effect of phospholipase A on ATPase systems of subcellular fractions of brain. In: Nervous System (ed. Leningrad University), 1980: 21, 20-24.
3. Prokudina E.A., Bazanova N.V., Borovitskaya A.E., Vershinina S.F. Purine derivatives used to normalize the hypothalamus function of rats exposed to gamma-neutron radiation. Radiobiologia, 1984: 24, 534-536.
4. Prokudina E.A., Basanova N.V., Borovitskaya A.E. Meradine used to normalize the catecholamine metabolism in rat hypothalamus at later times after irradiation. Radiobiologia, 1984: 25, 103-105.
5. Borovitskaya A.E., Prokudina E.A., Kadyrova N.O., Rzhonsnitskaya L.P. Therapeutic effect of purine derivatives on the life span of irradiated animals. Radiobiologia, 1987: 27, 653-656.
6. Evtushenko V.I., Borovitskaya A.E., Hanson K.P. The influence of irradiation on gene expression in rat thymus and brain. Radiobiologia, 1990: 22, 164.
7. Borovitskaya A.E., Hanson K.P., Evtushenko V.I. The effect of prenatal neutron irradiation on the expression of the genes coding for myelin proteins and neuronal surface glycoproteins in developing rat brain. Proceedings of the Russian Academy of Science, 1990: 315, 1482-1484.
8. Borovitskaya A.E., Evtushenko V.I., Hanson K.P. Effects of prenatal neutron irradiation on the gene expression in developing rat brain. Voprosi Medizinskoj Chimii, 1991: 37, 25-30.
9. Vekovischeva O.I., Blagova O.E. Borovitskaya A.E., Evtushenko V.I., Hanson K.P. Behavioral changes in adult rats irradiated in utero. Proccedings of the Russian Academy of Science, 1992: 324, 6, 1323-1326.
10. Borovitskaya A.E., Evtushenko V.I., Tokalov S.V., Yagunov A.S., Hanson K.P. Fluorocytometric analysis of cell population in prenatally irradiated rat brain. Proceedings of the Russian Academy of Science, 1994: 334, 5, 646-648.
11. Borovitskaya A.E., Evtushenko V.I., Hanson K.P. Effect of prenatal neutron irradiation on gene expression in developing rat brain. In: Radiobiological Disasters: Consequences of Accidents at Nuclear Power Stations (ed. E.B.Burlakova), Nova Science Publishers, Inc., New York, 1995: 103-114.
12. Borovitskaya A.E., Evtushenko V.I., Steven L.Sabol. Gamma-radiation-induced cell death in the fetal brain possesses molecular characteristics of apoptosis and is associated with specific messenger RNA elevations. Molecular Brain Research, 1996: 35, 1-2, 19-30.
13. Jia Lu, Qing Li, Hong Xie, Zhi-Jian Chen, A.E. Borovitskaya, Noel K. MacLaren, Abner L.Notkins and Michael S.Lan. Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in IDDM: Relationship to IA-2 and the 37/40 kDa tryptic fragments. Proc. Natl.Acad.Sci. USA, 1996: 90, 2307-2311.
14. Quing Li, Anna Borovitskaya, Mark DeSilva, Clive Wasserfall, Noel K. MacLaren, Abner L.Notkins and Michael S.Lan. Autoantigens in Insulin-dependent Diabetes Mellitus: Molecular Cloning and characterization of Human IA-2 beta.
Proceedings of the Association of American Physicians. 1997: 109, 4, 429-439.
15. Anna E. Borovitskaya and Peter D'Arpa. Replication-dependent and -independent camptothecin cytotoxicity of seven human colon tumor cell lines. Oncology Research, 1998:V.10, pp.271-276.
16. G. Han, K. Gable, L. Yan, M. Natarajan, J. Krishnamurthy, S. Gupte, A. Borovitskaya, J. Harmon, T. Dunn. The Topology of the Lcb1p Subunit of Yeast Serine Palmitoyltransferase. The Journal of Biol. Chem. 2004: 279, 51, 537**-*****.
17. Anna Reeves, Marianna Sultanov, Seema Gupta, Mohammed M. Shareef, Mohammed Mohiuddin and Mansoor M. Ahmed. Inhibition of TGF-β signaling in normal lung epithelial cells confers resistance to ionizing radiation. International Journal of Radiation Oncology, Biology, Physics, 2007: 68, 1, 187-195.
18. Anna E. Reeves, Sabapathi Sathishkumar, Mansoor M. Ahmed. Radiation-induced apoptosis in pancreatic adenocarcinoma is augmented via Smad-4-mediated induction of Bax (in preparation).
19. Anna E. Reeves, Serguei Vinogradov, Eric Wickstrom, Balaji Panchapakesan, Mansoor M. Ahmed. Curcumin-nanogels and curcumin-carbon nanotubes as more efficient formulation of curcumin, a popular anticancer dietary spice (in preparation).
20. Sigrid A. Rajasekaran , Thu P. Huynh, Daniel Wolle, Cromwell Espineda, Anna Kvyatkovskaya, Landon Inge, Jeffrey F. Harper, James M Leatherman, James Mullin, Anna Reeves, Mansoor M. Ahmed and Ayyappan K. Rajasekaran NA+, K+-ATPase is a target of TGF-beta-induced epithelial to mesenchymal transition (in revision, Experimental Cell Research).
21. Sita D. Gupta, Kenneth Gable, Gongshe Han, Anna Borovitskaya, Luke Selby, Teresa M. Dunn, and Jeffrey M. Harmon. TSC10p and FVT1: Topologically distinct short chain reductases required for long-chain base synthesis in yeast and mammals. J. Lipid Res., Jan 2009; doi:10.1194/jlr.M800580-JLR200
22. EGR-1 forms a complex with YAP-1 and upregulates Bax expression in irradiated prostate carcinoma cells. Zagurovskaya M, Shareef MM, Das A, Reeves A, Gupta S, Sudol M, Bedford MT, Prichard J, Mohiuddin M, Ahmed MM. Oncogene. 2009 Jan 12. [Epub ahead of print]
PRESENTATION AT MEETINGS
1986 - Meeting of European Society on Environmental Carcinogenesis, Varna, Bulgaria
(oral presentation)
1986 - International Agency for Research on Cancer, Conference on Perinatal and Multigeneration Carcinogenesis, St. Petersburg, Russia (oral presentation)
1989 - First All-Union Radiobiological Congress (Symposium Lecture), Moscow, Russia
(oral presentation)
1990 - First International Conference on Genetic Consequences of Chernobyl Nuclear Disaster, Green Cape, Chernobyl, Russia (Symposium Lecture),
1991 - International Conference on Long-Term Effects of Nuclear Plant Disasters, Moscow, Russia (Symposium Lecture)
1992 - Gordon Research Conference on Cancer, Newport, RI, USA (poster)
1993 - AACR Special Conference "Cell Death in Cancer and Development", Chatham (Cape Cod), MA, USA (poster)
1993 - 23rd Annual Meeting of Society for Neuroscience, Washington, D.C., USA (poster)
1994 - ASBMB Fall Symposium "Genetic and Biochemical Approaches for Studying Cell Death", Lake Tahoe, Calif., USA (poster)
2004 - Experimental Biology "Translating the Genome", Washington, DC (poster)
2005 - Radiation Research Society Annual Meeting, Denver, CO (poster)
2005 - American Association for Cancer Research Annual Meeting, Washington, DC (2 posters).
2006 - Radiation Research Society, 53rd Annual Meeting, Philadelphia, PA (poster)
2007 - American Association for Cancer Research Annual Meeting, Los Angeles, CA, (poster)
2007 - International Congress of Cancer Research, San Francisco, CA, (poster)
2008 - "Era-of-Hope" Breast Cancer Program (DOD) meeting, Baltimore, MD (poster)
References
Mansoor M. Ahmed, Associate Professor
University of Miami
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Eric Wickstrom, Professor of Biochemistry & Molecular Biology
Thomas Jefferson University
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Jeffrey M. Harmon, Professor and Chair, Department of Pharmacology
USUHS
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Teresa Dunn, Professor and Chair, Department of Biochemistry
USUHS
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