Research Scientist,

Research&Development,

Indian Immunologicals limited,

Gachibowli, Hyderabad. 500032.

India.

092******** (Mobile)

qmbhxq@r.postjobfree.com (E-Mail)

Complete Home Address

DD1-306, Aarambh township,

Rajeev Swagruha,

Bapi reddy colony, Chandanagar,

Hyderabad. 50019.

CAREER OBJECTIVE:

To learn, grow and contribute in an environment where I can employ my knowledge and research skills for development of novel agents or procedures that could be used for therapeutic and vaccine purposes for the treatment and prevention of diseases affecting human and animal health and also invention of new methods for diagnosis and early detection of causative agents of such diseases.

PROFESSIONAL QUALIFICATIONS

The Jawaharlal Nehru Technological University, Hyderabad. (2005-2010)

Ph.D. Thesis Research Conducted at Indian Immunologicals Limited

Ph.D., Institute for Science and Technology, The Jawaharlal Nehru Technological University, Hyderabad 500082.

Specialization: Molecular Biology, Biotechnology, Protein chemistry, Protein purification and Immunology.

Field of Study (Ph.D.,): Development of recombinant vaccine for animal and human diseases

Ph.D., Thesis Topic: “ Development of a recombinant tick vaccine”

Advisor: Prof. M.Lakshmi Narasu

University of Madras - Chennai, India

1 Master of Science, Field of Study (M.Sc.,): Biochemistry (1999-2001)

M.Sc., Thesis Topic: Effect of various ulcerated drugs on the activity of Adenosine De aminase.

Advisor: Prof. K.A. Balasubramaniam, M.Sc., PhD.,

Specialization: Biochemistry, Molecular biology, Enzyme kinetics, Clinical Biochemistry, Analytical Biochemistry, Hormonal regulations, Immunology and Biotechnology.

2 Bachelor of Science (B.Sc.,) : Biochemistry (1995-1998)

Specialization: Biochemistry, Molecular biology, Enzyme kinetics, Clinical Biochemistry, Analytical Biochemistry

RESEARCH EXPERIENCE

Position held Department / Institute Period

Research Executive 2 Molecular Biology lab,

Research and Development Department,

Indian Immunologicals Limited,

Hyderabad. 01.04.2010 to date

Research Executive 3 Molecular Biology lab,

Research and Development Department,

Indian Immunologicals Limited,

Hyderabad. 01.04.2007 to 31.03.2010

Research Officer 1 Molecular Biology lab,

Research and Development Department,

Indian Immunologicals Limited,

Hyderabad. 18.09.2004 to 31.03.2007

Senior Research Fellow Prof. P N Rangarajan’s lab,

Department of Biochemistry,

Indian Institute of Science,

Bangalore. 01.06.2003 to 14.09.2004

Junior Research fellow Prof. P N Rangarajan’s lab,

Department of Biochemistry,

Indian Institute of Science,

Bangalore. 01.06.2002 to 31.05.2003

Junior Research fellow Prof. Ram Rajasekharan’s lab,

Department of Biochemistry,

Indian Institute of Science,

Bangalore. 04.02.2002 to 31.05.2002

RESEARCH SKILLS

1 Over 10 years of experience in the field of Yeast Molecular biology (Pichia pastoris and saccharomyces cerevisiae), cloning, expression and purification of different recombinant heterlogous proteins from Yeast Pichia pastoris, Saccharomyces and E.coli

2 Protein purification, characterization and downstream processing.

3 Extremely talented in conventional protein purification through different chromatographic methods.

4 Experienced in recombinant protein purification from E.coli and Yeast.

5 Extensive hands-on experience in performing various molecular biology techniques such as DNA and RNA isolation, molecular cloning, reverse transcription and polymerase chain reaction (RT-PCR), Real time RT-PCR, genome sequencing and sequence analysis

6 Proficient in immunological and techniques such as enzyme linked immunosorbent assay, western blotting and confocal microscopy.

7 Experienced in preparation of primary cell cultures and propagation of cell lines both monolayer and suspension cultures

8 Adept in expression of protein in prokaryotic system

9 Experienced in operating software applicable to the field of molecular biology like Clustal, GeneDoc, Recombination event detection softwares.

SKILLS IN DOCUMENTATION IN QUALITY CONTROL AND QUALITY ASSURANCE :

1. Well experienced in making the documentation of technology transfer to production, Quality control and Quality assurance department.

2. Experienced in preparing SOPs and STP’s to other departments in the projects and products worked in the lab.

3. Interaction between QC and QA departments in the issues related to technology transfer of products that worked out in the lab.

MANAGEMENT SKILLS

1 Strong organizational skills.

2 Managed a team of 6 people for 5 years in different scientific projects.

3 Proven ability to handle multiple projects simultaneously.

4 Solid ability of Conceptual thinking, and proficiency in group management.

5 Strong knowledge and practice of project management.

6 Guided more than 10 project students in their Master’s.

EXTRA RESPONSIBILITIES:

1 Develop and implement project plans.

2 Manage project timelines and responsible for project completion.

3 Prepare and present results in reports update Management or Project Team members.

4 Maintain awareness of relevant areas of research and competitive activity through reading the literature and occasional attendance of appropriate scientific meetings.

5 Perform a broad range of biological tests and procedures for viruses and yeast in the BSL-2 and BSL-3 labs applying in vitro techniques.

6 Managed a team successfully for different scientific projects.

7 Interaction between other departments like purchase and stores related to our lab activities.

PROJECTS GUIDED FOR STUDENTS UNDERGOING MASTERS PROGRAM WITH VARIOUS UNIVERSITIES IN INDIA.

1 Cloning , expression and purification of interferon gamma in E.coli,

2 Optimization of Bm95 expression in different yeast media.

3 Cloning , expression and purification of PPRV N protein in E.coli

4 Conventional purification of clostiridal epsilon toxin B

5 Recombinant expression and purification of Mycobacterial ESAT6 protein

6 Recombinant expression and purification of Mycobacterial 35kDa protein

7 Recombinant expression and purification truncated PPRV N protein

8 Recombinant expression of Ag85a of Mycobacterium.

9 Recombinant expression and purification of mycobacterium tuberculosis fusion proteins.

10 Development of recombinant HSP65 protein of mycobacterium bovis.

PROJECTS INVOLVED:

1. Development of Recombinant Tick vaccine in Yeast. (Ph.D. Project).

This Project involved in the cloning, expression and conventional purification of recombinant Bm95 antigen of R.microplus tick parasite. The recombinant vaccine was developed in Yeast pichia pastoris and saccharomyces cerevisiae .

2. Development of recombinant vaccine in Yeast and diagnostic tools for bovine Tuberculosis in cattle.

This Project involved in the cloning, expression and purification of different recombinant proteins for vaccine target and diagnostic tool for Mycobacterium tuberculosis in E.coli and Pichia pastoris

3. Construction of chimeric full length cDNA of a specific virus. (Novel vaccine stratergy).

This Project involved in the construction of full length viral cDNA in to expression vector. The Full length viral RNA was made by in vitro transcription and infected to cell line to recover a complete live virus.

4. Development of Yeast expressed recombinant vaccine against porcine cysticercosis in porcine and human.

This Project involved in the cloning, expression and conventional purification of recombinant TSOL18 antigen of Taenia solium parasite. This recombinant protein was expressed and secreted in to medium. The recombinant vaccine was developed in Yeast pichia pastoris.

5. Development of Yeast expressed recombinant vaccine against hookworm infections in human.

This Project involved in the cloning, expression and conventional purification of recombinant Aspartic protease antigen of human hookworm Necator Americanus. This recombinant protein was expressed and secreted in to medium. The recombinant vaccine was developed in Yeast pichia pastoris.

6. Development of VLP (Viral coat protein) based canine vaccines and designing of diagnostic kit.

This Project involved in the cloning, expression and conventional purification of recombinant VLPs exposing the immunodominant epitopes of 2 different canine viruses. The purified recombinant protein used for development of diagnostic kit.

7. Development of recombinant vaccine against enterotoxaemia in cattle.

This project involved in the development of recombinant vaccine against enterotoxaemia in E.coli for sheep.

8. Development of recombinant viral vectors.

The development of different Poxviruses as recombinant viral vectors, which carry the antigen targets and their efficiency, was analyzed in animals.

9. Analysis of Cis and Trans acting elements of AOX promoter in pichia pastoris

Investigated the regulatory mechanism of alcohol oxidase promoter in methylotrophic yeast Pichia pastoris. The project involved in the analysis of different cis acting and trans acting elements that involved in the regulation of AOX promoter in Yeast Pichia pastoris. A specific protein that binds to the AOX promoter during methanol induction was identified. The same protein was not bound to the promoter during glycerol as the carbon source. The protein was furthur charactgerized biochemically in detail.

On going project for combination Hepatitis B and Pneumococcal vaccine :

The development of combinatorial recombinant vaccine (Hepatitis B+ S.Pneumococcus) for human. The Pneumococcal epitopes will be displayed in the Hepatitis B surface antigen and the purified Hepatiits B S antigen will be analyzed for immunogenicity and efficacy against Hepatitis B infection as well as S.Pneumococcal infection.

PRODUCTS CONTRIBUTED

1. Recombinant tick vaccine:

Individually contributed to the development of recombinant tick vaccine and is ready to launch. This is first vaccine against specific tick species in the world.

2. Recombinant vaccine against cysticercosis.

Individually contributed to the development of recombinant vaccine for cysticercosis in porcine and human. The technology was already transferred to Production. All the QC and QA related documentation work has been completed.

3. Recombinant vaccine against Hookworm infections in human.

Individually contributed to the development of recombinant vaccine against hookworm infections. The project is in the final stages and technology will be transferred soon.

4. Recombinant vaccine for entero toxaemia in sheep.

Contributed and guided a group in the development of recombinant vaccine for enterotoxaemia in sheep.

EXPERTISE IN THE FIELD OF YEAST MOLECULAR BIOLOGY, E.COLI, RECOMBINANT PROTEIN EXPRESSION, PROTEIN PURIFICATION AND CHARACTERIZATION :

1. Yeast expressed recombinant TB (Mycobacterium Bovis) proteins.

More than 20 recombinant proteins of Mycobacterium bovis (E.coli expressed proteins) have been purified by affinity chromatography and the standardization at laboratory, downstream levels was completed. The purified proteins were supplied for the purpose of standardizing diagnostic kit for tuberculosis.

Around 5 recombinant proteins of Mycobacterium bovis (Pichia pastoris expressed proteins) have been purified by affinity chromatography and the standardization at laboratory, downstream levels was completed.

2. Yeast expressed recombinant tick antigen.

The recombinant tick antigen was purified from Yeast Pichia pastoris through conventional methods. The standardization at laboratory and downstream levels was completed and the technology was transferred to production unit. The vaccine has been approved by RCGM and DCGI of Govt. of India

3. Yeast pichia Pastoris expressed veterinary virus VLPs.

The purification of viral like particles from pichia pastoris, has been completed through conventional purification methods. This technology has been applied for patenting.

4. Purification of E.coli expressed plant VLPs

The conventional purification of Plant VLPs which exposes many antigenic epitopes was optimized at downstream levels and the proteins are transferred to specific labs.

5. Expression and Purification of recombinant cysticercosis antigen from pichia pastoris.

The recombinant vaccine candidate of cysticercosis was expressed as secretory form in yeast and the downstream process of purification was standardized. The technology is ready to transfer to production unit. The vaccine has been approved by RCGM of Govt. of India

6. Purification of recombinant epsilon toxin from E.coli.

The conventional purification recombinant toxin of (C.perfringens) expressed from E.coli was standardized and technology was transferred to production unit.

7. Expression and Purification of recombinant antigen for Hookworm infection from yeast.

The conventional purification of recombinant vaccine candidate for hookworm infections is partially completed. The optimization at downstream level is in progress.

8. Purification of Hepatitis B suface antigen from yeast.

The purification of Hepatits B surface antigen which displays the epitopes of S.Pneumococcus has been completed. The immunogenicity and safety studies will be initiated soon.

EXPERIENCE IN PROTEIN CHARACTERIZATION:

1. Characterization of Tick antigen.

The recombinant protein was characterized for its physical and chemical properties.

2. Characterization of recombinant protein of Taenia Solium.

The recombinant protein was characterized for its physical and chemical properties.

3. Characterization of recombinant epsilon toxin

The recombinant protein was characterized for its physical and chemical properties.

4. Characterization of Plant VLPs.

The recombinant protein was characterized for its physical and chemical properties.

TEACHING EXPERIENCE :

Worked as a part time lecturer in Acharya Nagarjuna distance education degree college in Hyderabad.

Time period 2 years

Specialization : Industrial Biotechnology, Microbiology, Microbial genetics, Biochemistry, Food and industrial microbiology, Analytical chemistry , Fermentation technology.

PUBLICATIONS

National Journals International Journals Products contributed Journals communicated Conference and Seminars Patents Authored

1 8 4 2 2 1

INTERNATIONAL PUBLICATIONS

1. Recombinant mid gut antigen (Bm95) as a vaccine against Indian Rhiphicephalus haemaphysaloides in Bos indicus cattle

Parthasarathy Sugumar, Dev Chandran , Gudavalli Sudha Rani , Pallichera Vijayan Shahana ,Dasarath Keshavrao Maske , Pundi Narasimhan Rangarajan , Lakshmi Narasu Mangamoori ,Villuppanoor Alwar Srinivasan , Research in Veterinary Science. 2011. 90 262-268.

2. Assessment of immunoprophylactic efficacy of recombinant midgut antigen (Bm95) of Rhiphicephalus microplus in Bos indicus.

Parthasarathy Sugumar, Jadhav Vishwanath Tukaram, Mukhulesh Gatne, DevChandran, Lakshmi Narasu Mangamoori, Pundi Narasimhan Rangarajan and Villuppanoor Alwar Srinivasan. Current Trends in Biotechnology and Pharmacy 2010. 4(4) 855-861.

3. Assessment of critical parameters in blood processing for bovine interferon-gamma ELISpot assay on detection of Mycobacterium bovis infected cattle.

Maroudam Veerasami, Loganathan Moorthy, Dipankar Das, Sugumar Parthasarathy, Dev Chandran, Rajendra Lingala and Villuppanoor Alwar Srinivasan. Veterinaria Italiana 2011. 47(1) 25-34.

4. MVA recombinants expressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge.

Dev Chandran • Kolli Bhaktavatsala Reddy • Shahana Pallichera Vijayan, Parthasarthy Sugumar • Gudavalli Sudha Rani, Ponsekaran Santha Kumar, Lingala Rajendra, Villuppanoor Alwar Srinivasan. Indian Journal of Microbiology. 2010 , 50 : 266-274.

5. Development of a Recombinant Epsilon Toxoid Vaccine against Enterotoxemia and Its Use as a Combination Vaccine with Live Attenuated Sheep Pox Virus against Enterotoxemia and Sheep Pox

Dev Chandran, Sureddi Satyam Naidu, Parthasarathy Sugumar, Gudavalli Sudha Rani, Shahana Pallichera Vijayan, Deepika Mathur, Lalit C. Garg, and Villuppanoor Alwar Srinivasan. CLINICAL AND VACCINE IMMUNOLOGY, 2010, 17, 1013–1016

6. Immune responses against recombinant tick antigen, Bm95, for the control of Rhipicephalus (Boophilus) microplus ticks in cattle.

Kumar A, Garg R, Yadav CL, Vatsya S, Kumar RR, Sugumar P, Chandran D, Mangamoori LN, Bedarkar SN. Veterinary Parasitology. 2009. 28;165(1-2):119-24.

7. Display of neutralizing epitopes of Canine parvovirus and a

T-cell epitope of the fusion protein of Canine distemper virus on chimeric tymovirus-like particles and its use as a vaccine candidate both against Canine parvo and Canine distemper.

Chandran D, Shahana PV, Rani GS, Sugumar P, Shankar CR, Srinivasan VA. Vaccine. 2009. Dec 10; 28(1):132-9.

8. Identification of Japanese encephalitis virusinducible genes in mouse brain and characterization of GARG39/IFIT2 as a microtubule-associated protein.

Sougata Saha, Parthsarathy Sugumar, Prakash Bhandari and Pundi N. Rangarajan. Journal of General Virology(2006) 87, 3285-3289.

NATIONAL PUBLICATIONS

9. Detection of IFN-γ responses in cattle using Recombinant TB antigens, ESAT-6 and CFP-10.

Jaya Bhavani, Dev Chandran, Maroudam Veerasami, Parthasarathy sugumar, Pallichera Shahana, Dipankar Das, Mangamoori lakshmi Narasu & Villuppanoor Alwar Srinivasan. Biotechnology, Bioinformatics and Bioengineering 2011, 1 (2):255-264.

AWARDS AND HONOURS

1 Received best poster presentation award in XXIV Annual Convention of Indian Association of Veterinary Microbiologists and Immunologists. An international conference on protecting animal health, held at Raipur, Chattisgarh, India on January 27-29, 2010.

CONFERENCES AND SEMINARS:

1 Participated in oral presentation at XXIV Annual Convention of Indian Association of Veterinary Microbiologists and Immunologists. An international conference on protecting animal health, held at Raipur, Chattisgarh, India on January 27-29, 2010.

2 Participated in the Indo American international conference on Avian Influenzae held at University of Hyderabad on September 2008,

PATENT AUTHORSHIPS:

1 Dev Chandran, Parthasarathy Sugumar, Singanallur Balasubramanian Nagendrakumar and Villuppanoor Alwar Srinivasan. Production of FMDV virus like particles (VLPs) in Yeast Pichia pastoris (IPA 216/CHE/2010; Application date 10.02.2010; Filing date 10.02.2010).

EXTRAMURAL FUNDING:

1 The project involved in the development of recombinant vaccine against porcine cysticercosis will be collaborated with GalvMed for funding.

Declaration

This is to certify that the information provided is true to the best of my knowledge and belief.

Yours faithfully,

(P.Sugumar)

Place: Hyderabad

Date:

LIST OF REFERENCES:

1. Dr. S.B.Nagendra kumar , M.V.Sc, Ph.D.

Research scientist,

FMDV Risk management project,

Australian animal health laboratories,

CSIRO livestock industries,

Melbourne,

Australia.

E-mail : qmbhxq@r.postjobfree.com

2. Dr. Thirunavukkarasu angappan , M.V.Sc,

Deputy General manager,

Panacea biotech,

Chandigarh. India.

E-Mail : qmbhxq@r.postjobfree.com

Ph : 089********.

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