Biotechnology

Viral Shah - CV

OBJECTIVE: To obtain full time challenging position for the advancement of acquired knowledge, skills and experience

TECHNICAL SKILL:

•In vitro experiments on rat ileum, Guiney pig trachea etc.

•Cell culture

•Lab techniques like streak plate technique for isolation, plate count, endotoxin testing, preparation of media, aseptic technique when culturing, handling and isolating microorganisms, positive and negative gram staining technique etc

•Hand on experience with PCR, RT-PCR, SDS PAGE, Western blot analysis, Gel Filtration chromatography, RFLP based DNA fingerprinting, micro array, cloning, incubator, autoclave, UV - VIS spectrophotometer, high throughput screening assay

•MS Office

MOLECULAR BIOLOGY PROJECTS, NYU POLY, NY: (Fall 2010)

•Gel filtration chromatography for DNA and RNA separation

•Restriction enzymes use as applied to RFLP-based DNA fingerprinting

•Microarrays as applied to functional human genomics

•Bacterial restriction-modification systems and site-specific restriction-methylation of DNA

•Mutations in the p53 tumor suppressor gene and its role in cancer

•RT-PCR and its relation with HIV replication

•Bacterial transformation by pGFP plasmid DNA

•Purification and characterization of the recombinant protein

CELL BIOLOGY PROJECTS, NYU POLY, NY: (Spring 2010)

•Isolation of human mitochondrial DNA and amplification using PCR

•Application of biotechnology to biomedical diagnosis related to sickle cell anemia

•Isolation of human DNA and comparison of DNA polymorphisms between individual

•To clone DNA fragment, extract and map resulting fragment from the recombinant plasmid

ACADEMIC PROJECTS, NYU POLY, NY:

•Hypothesis for HIV vaccine discovery (Spring 2011)

oFocus: HIV RNA Fragments

oHIV RNA sequencing and Profiling

oIdentify similar and different fragments belonging to different HIV varieties in one region (For instance one fragment exists in 687 varieties) and use them for trials

oLess work for Human Immune system against a fragment in compare with whole HIV RNA, low risk, Patient repetition is possible for different fragments in clinical trial

oManufacturing and launch

•Oral presentation on Microarray and its applications (Fall 2010)

•Developed Biosensor Theory to detect VZV virus (Fall 2009)

•Teaching Assistant: Developed demonstrations as well improved lab manual for chemistry courses (Spring 2011 – Fall 2009)

•Research Assistant: Evaluation for Mechanism of Action of an aqueous extract of Ficus glomerata leaf, as an Anti-Ulcer drug belonging to Moraceae Family using rat ileum (Spring 2009)

•Poster presentation on Evaluation of the effect of Brassica oleraceae on bronchial hyper reactivity (Fall 2008)

EDUCATION:

MS Biotechnology, Polytechnic Institute of NYU, Brooklyn, NY, USA

Graduated: May 2011, Current GPA: 3.4

Honors: Semifinalist in inno/vention competition presented by Time Warner Cable during spring 2011 at NYU Poly, Research Scholarship at NYU Poly

REFERENCES: (Available on request)

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