Molecular biology, Microbiology, Virology and Cell culture.

CURRICULUM VITAE

BALAJI KASA

C/O - G.RAVIDRA REDDY

D.NO-2/31/1/1/B/3/1

SHIVAJI NAGAR

TARANAGAR E-mail: ******.****@*****.***

SHERILINGAMPALLY

HYDERABAD - 500019 Mobile: +91-949*******

CAREER OBJECTIVE

To work as a professional in an organization that offers opportunities to implement my present knowledge while playing an active role in the growth of the organization and also offers avenues for further learning. Enthusiastic to play a key role in challenging works in creative way.

PROFESSIONAL SUMMARY

I have substantial 4 years of biotech industry (R&D) in the field of Molecular biology, Microbiology, Virology and Cell culture. I have contributed actively in the process of expression and purification of various heterologous protein in Bacterial and yeast expression Platform. Expertise in the maintenance, propagation and characterization of Bacterial and yeast cell cultures. Hands own experience in adaptation, propagation and characterization of animal viruses.

EXPERIENCE 4 YEARS (JULY 2008 –TILL DATE)

Organization Designation Duration

Indian Immunologicals Limited, Hyderabad Research officer August 2009 - Till to date

Sandor proteomics Pvt. Ltd., Hyderabad Research officer July 2008 - August 2009

EDUCATIONAL QUALIFICATIONS

2008 M.Sc. Biochemistry from Andhra University -67%

2006 B.Sc. Microbiology, Biochemistry, Medical lab technology from Andhra University-75%

2003 Intermediate Microbiology, Biochemistry and Medical lab technology from Board of

Intermediate, A.P -80%

JOB PROFILE

Molecular Biology: Cloning and expression of heterologous proteins in Bacteria, Yeast and Mammalian expression systems. Standardization of PCR and RT-PCR, Assembly of genes using SOE PCR method, DNA sequencing, Genomic DNA isolation (From different sources), Total RNA isolation and Plasmid DNA isolation. Downstream process purification of recombinant proteins using chromatographic techniques.

HPV prophylactic vaccine:

• Cloning and expression of HPV L1 (16L1,18L1,31L1,45L1 and 52L1) capsid proteins in

Pichia pastoris. Assembled VLPs purified by affinity chromatography. Immuno efficacy trails with bi and pentavalent VLP formulation in mice.

• Tandem cloning of HPV16 L1 and L2 in pichia pastoris.

• Cloning and expression of HPV L1 (16L1 and 18L1) in Salmonella Typhi Ty21a.

Transnasal immunization followed by CMI response in mice was evaluated by cell based

assays.

• Cloning of HPV genes in mammalian expression systems.

• Periplasmic expression of HPV proteins in Bacteria.

• Preparation of HPV Pseudovirions for determining the sera titer in vaccine efficacy studies.

Coccidial Vaccine: Expression of Coccidial proteins (EtMIC-1 and EtMIC-2) in tobacco by

Agro infiltration.

Microbiolgy: Bacterial strains like DH5α, BL21 and Salmonella isolation propagation and maintenance, biochemical characterization and pure culture development.

cGMP Seed Banks: Preparation of Recombinant HPV salmonella 16L1 and 18L1 master and working banks and played a vital role in technology transfer to production department.

Virology : Isolation of viruses (Bovine herpesvirus -1) from clinical samples, adaptation of virus isolates to in vitro mammalian cell culture, propagation, plaque assay, purification, TCID50, filtration/concentration, characterization of viruses (BHV-1) for vaccine development, , virus bank preparation.

IBR Vaccine: Evaluation of inactivated oil and Al-gel BHV-1 vaccine’s against Bovine herpesvirus-1 (BHV-1) in cattle. Studies on Humoral and Cell mediated immune response in IBR vaccinated cattle by ELISA, MNT, FACS and ELISPOT.

Cell culture : Maintenance and characterization of mammalian cell lines (BHK-21, VERO, MDBK, MDCK, MRC5,293TT etc.,) Isolation and cryo-preservation of PBMC’s and Splenocytes from vaccinated and natural infected animal for the study of cell mediated immune response (Cytokine Profile) against different antigen stimulants. Transfection in mammalian cells by lipofectamine.

TECHNICAL SKILLS

Purification of recombinant proteins using

IMAC

Ion exchange chromatography

Affinity Chromatography

Gel filtration chromatography

HPV proteins (VLPs ) characterization by using pseudovirion neutralization assay

Characterization of proteins using SDS-PAGE, Western blotting and ELISA

Quantification of Cytokines using Real time PCR and ELISPOT analysis

Sorting of CD4 and CD8 cells using Magnetic Associated Cell Sorter (MACS)

Maintenance , Propagation and Characterization of microbial cultures

Agro infiltration and transient expression of heterologous genes in tobacco.

HANDLING EXPERIMENTAL ANIMAL MODELS

Experience on maintenance and bleeding of laboratory animals’ viz. Rabbits, mice and chicks.

PUBLICATIONS

1. Kota Sathish, Rajan Sriraman, B. Mohana Subramanian, N. Hanumantha Rao, Balaji Kasa, Jagan Donikeni, M. Lakshmi Narasu, and VA Srinivasan (2012). Plant expressed coccidial antigens as potential vaccine candidates in protecting chicken against coccidiosis. Vaccine (Published Online).

2. K. Sathish, R. Sriraman, B. Mohana Subramanian, N. Hanumantha Rao, K. Balaji, M. Lakshmi Narasu, and V.A. Srinivasan (2011). Plant expressed EtMIC2 is an effective immunogen in conferring protection against chicken Coccidiosis. Vaccine, 29: 9201– 9208.

PAPER PRESENTATIONS

1. Cell mediated immune response in mice to human papilloma virus genotype 16 E7 delivered using Salmonella enterica Serovar typhi strain Ty21a. Ponnanna N.M, Rajan Sriraman, Ravi Ranjan Verma, Swapna E, K. Balaji, K.Sathish, V. Maroudam, R.K. Sisodiya and V.A Srinivasan.(AOGIN-India National Conference November 5-6, 2011 Bandra)

2. Binding and neutralization efficiencies of monoclonal antibody (18E5.G3) for L1 capsid of HPV-18VLP. Pitta Vidyasagar, Rajan Sriraman, Rajalakshmi. S, Lakshmipriya. V, Balaji. K, Sivakumar. V, Hanumantha Rao. N, Ravi Ranjan Verma, Abhinay. G, & Srinivasan. V.A.

(Souvenir International Symposium on Innovations in Free Radical Research and Experimental Therapeutics, December 7-9, 2011, Coimbatore.)

3. Cytokine profile of Human Papilloma Virus 16 (HPV 16) E7 oncoprotein specific T-cell response on immunization of mice with recombinant Salmonella enterica serovar Typhi. Ponnanna N.M, Rajan Sriraman, Ravi Ranjan Verma, K. Balaji, and V.A Srinivasan.

(Virocon-2011 National Research Centre on Equines, December 29-31, 2011, Hisar)

PERSONAL PROFILE

Name : Balaji Kasa

Father’s name : K. V. V. Satyanarayana

Date of Birth : 10- 06- 1984

Languages known : English, Hindi and Telugu

Contact Address : Balaji Kasa

C/O -G. Ravindra Reddy

D.NO-2/31/1/1/B/3/1

Shivajinagar, Taranagar

Serilingampally

Hyderabad – 500019

DECLARATION

I do hereby declare that the above furnished information is true to the best of my knowledge. Yours Sincerely,

(Balaji Kasa)

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