Biologist

LIN YAN, Ph.D.

**** **** *** **, **** • Seattle • WA 98105 • 206-***-****• q9ijr3@r.postjobfree.com

OBJECTIVE

To contribute my strong biomedical research experience to the biotech or pharmaceutical company

SUMMARY OF QUALIFICATIONS

• Nine years of research experience in immunology, biochemistry, cell and molecular biology

• Productive scientific achievements proven by high-quality publication and grant awards

• Proficient in project planning & organizing, and data analysis & reporting

• Proven solid presentation, communication, teamwork and leadership skills

EDUCATION

• Ph.D. in Molecular Biology, China 2005

• B.S. in Biotechnology, China 2001

PROFESSIONAL EXPERIENCES

Senior Fellow 11/2009 – present

Dept. of Bioengineering, College of Medicine, University of Washington, Seattle, WA

• Initiated an NIH R01 project on the protein, cDNA and mRNA vaccine development against healthcare-associated bacterial biofilm infections

• Took charge of isolation/maturation of macrophage and dendritic cells, and tissue culture functional assays.

• Mentored postdocs and graduate students on the fundamental laboratory techniques and skills in molecular biology, microbiology and immunology

Postdoctoral Research Associate 08/2005 – 11/2009

Dept. of Microbiology & Immunology, College of Medicine, University of Illinois, Chicago, IL

• Led a team of three members to develop two bio-defense vaccine vector platforms, and evaluated vaccine immunogenicity and protection against MTB, vaccinia or HIV

• Investigated immune modulation ability of bacterial flagellin, the Toll-like receptor 5 ligand protein, in the in-vitro and in-vivo assays

• Supervised two graduate students, three summer interns and two technicians through basic lab work to project progress

Research Assistant 07/2001 – 08/2005

National Laboratory of Microbiology, Huazhong Agriculture University, Wuhan, China

• Engineered expression and purification of recombinant cytokine interleukin -2 and -6 in Bacterium (E. coli), Yeast (P. pastoris) and mammalian cells

• Characterized bioactivity of recombinant cytokines

• Evaluated immune stimulation effects of recombinant cytokines in mice, rabbit and pigs

SKILLS

Cell Biology and Immunology

• BSL-2 cell and tissue culture

• Enzyme-linked immunosorbent spot (ELISPOT)

• Enzyme-linked immunosorbent assays (ELISA)

• Flow cytometry assays

• Immuno-florescence protocols

Animal Assays

• Various routes of vaccine/drug administration: intraperitoneal, subcutaneous, intramuscular, intranasal or intravenous injection

• Retro-orbital& tail bleeding at various time points

• Aseptic surgery, euthanasia, necropsy, gross physical examinations

Molecular Biology & Biochemistry

• Polymerase chain reaction (PCR), RT-PCR, Real-time PCR

• Recombinant plasmid development

• Recombinant protein expression (in E. coli, yeast and mammalian cells) and purification

• Protein detection assays: SDS-PAGE and Western blot

Other Skills

• Computer applications: MS Word, Excel, PowerPoint, PhotoShop, etc.

• Statistical software use: student t-test, One-way ANOVA, GraphPad Prism

• Excellent written and verbal communication skills and strong presentation skills

• Fluent in English and Mandarin

AWARDS

• Travel grant for the National Summit on Gender and the Postdocorate, Philadelphia, PA. 03/2010

• Outstanding Ph.D. Dissertation Award from over 1000 nominees of all universities in China, Department of Education, China 07/2007

• An International grant “Expression of Porcine Interleukin-2 and Interleukin-6 in Pichia Pastoris and Their Adjuvant Effects on PRV Vaccines” (NO B/3528-1), honored by International Foundation for Science (IFS), Sweden 04/2006

PROFESSIONAL SOCIETY MEMBERSHIPS

• Sigma Xi, the Scientific Research Society

• American Society for Microbiology (ASM)

• Society for Mucosal Immunology (SMI)

• Association for Woman in Science (AWIS)

• National Postdoctoral Association (NPA)

PUBLICATIONS

• Yan, L., He, Q., Chen, H., Xiao, S., Wu, M., Lu, J. and Han, L. Expression of Porcine Interleukin-2 and Porcine Interleukin-6 and their Adjuvant Effects on Gene Deleted Vaccine of Pseudorabies Virus (TK-/gG-/LacZ+). Agriculture Sciences in China. 2003, 36 (10): 1213-1218. (English version, in same journal, 2003, 2 (8): 924-929)

• Yan, L., Qin, Y., Bei, W., Yu, X., Huang, H., and Chen, H. Expression of an Interleukin-6 - Interleukin-2 Fusion Protein (pIL-6-IL-2) in P. pastoris. European Cytokine Network, 2004. 15(3): p. 240-246.

• Yan, L., Qing, L., Bei, W, Huang, H., Chen, H. Expression of a Fusion Protein pIL6-IL2 in E. coli and Bio-activity Assay. Chinese Jounral of Biotechnology. 2004, 24(11): 38-42.

• Yan, L., Mu, F., and Chen, H.. The Adjuvant Effects of Recombinant pIL-2 on Inactivated Pseudoeabies Virus Vaccine in a Murine Model. Animal Biotechnology Bulletin. 2004, 9 (1): 401-406.

• Yan, L., Chen, H., Qin, Y., He, Q., Xiao, S. The Expression of Porcine Interleukin-2 in Pichia pastoris. Acta Veterinaria et Zootechnica Sinica. 2004, 35(2):208-212.

• Yan, L., He, Q., Yu, X., Bei W., and Chen, H.. The Co-administrating of Recombinant Porcine IL-2 Could Enhance Protective Immune Responses to PRV Inactivated Vaccine in Pigs. Vaccine. 2005, 23(35): 4436-4441.

• Yan, L, Gu, P., and Chen, H.. Expression of Porcine Interleukin-6 in Pichia pastoris. Acta Microbiologica Sinica. 2005, 45 (3): 387-391.

• Bei, WC., He, Q., Yan, L., and Chen, HC.. Construction and Characterization of A Live, Attenuated Apxiica Inactivation Mutant of Actinobacillus Pleuropneumoniae Lacking A Drug Resistance Marker. FEMS Microbiology Letters. 2005, 243(1): 21-27.

• Yu, X., Xiao, S., Fang, L., Yan, L., Dong, X., Chen, H.. High Expression of The Foot-and-Mouth Disease’S Structural Protein P1 in Escherichia Coli and Analysis of its Biology Activity. Chinese Jounral of Biotechnology. 2005, 21(1): 163-166.

• Zhao, W., Xiao, S., Fang, L., Jiang, Y., Song, Y., Yan, L., Yu, X., Chen, H. Immunogenicity of DNA Vaccine Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus Fused With VP22 of Bovine Herpesvirus 1. Chinese Jounral of Biotechnology. 2005, 21(5):725-730.

• Zhao, W., Xiao, S., Fang, L., Jiang, Y., Song, Y., Yan, L., Yu, X., Chen, H. Construction of the Recombinant Pseudorabies Virus TK-/gE-/VP22GP5+ Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus Fused with VP22 of Bovine Herpesvirus 1. Chinese Journal of Virology. 2006, 22(1):62-65.

• Bei, W., Yan, L., He, Q., Xiao, S., and Chen, H.. Eukaryotic Expression and DNA Immunization of apx II A Gene of Porcine Actinobacillus pleuropneuromoniae. Journal of Agricultural Biotechnology. 2005, 13(5): 616-619.

• Huang, H., Zhou, R., Fan, H., Dan, H., Chen, M., Yan, L., Bei, W., and Chen, H.. Generation of Monoclonal Antibodies and Epitope Mapping of Apxiva of Actinobacillus Pleuropneumoniae. Molecular Immunology. 2006, 43(13): 2130-2134.

• Zhao, W., Xiao, S., Fang, L., Jiang, Y., Song, Y., Yan, L., Yu, X., Chen, H.. Construction of Recombinant Pseudorabies Virus Coexpressing E and M Protein of Porcine Reproductive and Respiratory Syndrome Virus Fused with VP22 Protein of Bovine Herpesvirus 1 and Its Immunogenicity in Mice. Acta Veterinaria Et Zootechnica Sinica. 2006, 37(4): 401-407.

• Bei, W., He, Q., Zhou, R., Yan, L., Huang, H., and Chen, H.. Evaluation of Immunogenicity and Protective Efficacy of Actinobacillus Pleuropneumoniae HB04C(-) Mutant Lacking a Drug Resistance Marker in The Pigs. Veterinary Microbiology. 2007, 125(1-2): 120-127.

• Yan, L., Qiu, J., Chen, J., Ryan-Payseur, B., Huang, D., Wang, Y., Rong, Melton-Watt, L. , Freitag, N., and Chen, Z.. Selected Prfa* Mutations in Recombinant Attenuated Listeria Monocytogenes Augment Expression of Foreign Immunogens and Enhance Vaccine-Elicited Humoral and Cellular Immune Responses. Infection and Immunity. 2008, 76(8): 3439-3450.

• Zeng, G., Chen, J., Zhong, L., Wang, R., Jiang, L., Cai, J., Yan, L., Huang, D, Chen, C., and Chen, Z.. NSOM- and AFM-based Nanotechnology Elucidates Nano-Structural and Atomic-Force Features of a Y. pestis V Immunogen-Containing Particle Vaccine Capable of Eliciting Robust Response. Proteomics. 2009, 9(6): 1538-47

• Zhong, L., Zeng, G., Lu, X., Wang, R., Gong, G., Yan, L., Huang, D., and Chen, Z. NSOM/QD-based direct visualization of CD3-induced and CD28-enhanced nanospatial co-clustering of TCR and co-receptor in nanodomains during T-cell activation. PloS ONE. 2009, 4(6): e5945

• Ali, Z., Yan, L., Plagman, N., Villinger, F., & Chen, Z. (2009). T-cell immune manipulation during chronic phase of simian HIV infection confers immunological benefits. Journal of Immunology, 183, 5407-5417.

• Qiu, J., Yan, L. (co-first author), Huang, D., Leenhouts, K. and Chen, Z. (2011) Intranasal vaccination with recombinant Listeria ΔactA prfA* elicited robust systemic and pulmonary cellular responses, and secretory mucosal IgA. Clinical and Vaccine Immunology. (In publish, CVI. 00254-10V1)

• Bridgett Ryan-Payseur, Zahida Ali, Dan Huang, Lin Yan, Richard C. Wang, William E. Collins, Sakina Petiwala, Yunqi Wang, and Zheng W. Chen. Virus infection stages and distinct Th1 or Th17/Th22 T-cell responses in malaria/SHIV coinfection contribute to different outcomes of disease. Plos Pathogen. 2011 (In Review)

• Yan, L., Qiu, J., and Chen, Z. Comparative Evaluation of Immunization Route-Related Immune Response to Listeria monocytogenes-Based Vaccines in Mice. Vaccine. 2011 (In Review)

CONFERENCE PRESENTATION:

• Yan, L., Chen, H.. Research Proceeding on Porcine Interleukin-2 and Interleukin-6 as vaccine adjuvant. New Advances on Prevention and Control of Swine Severe Infectious diseases - the 5th National Chinese Veterinary Medical Association Annual Conference. 2002.

• Yan, L., Mou, F., and Chen, H.. The Adjuvant Effects of Recombinant pIL-2 on Inactivated Pseudoeabies Virus Vaccine in a Murine Model. The 10th Annual Conference of the Animal Infectious Disease Division, Chinese Veterinary Medical Association. 2003.

• Yan, L., Chen, H.. The Application of Cytokines in Veterinarian. Proceeding on Disease Prevention and Control in National Large-Scale Veterinary Farms - the 6th National Chinese Veterinary Association Annual Conference. 2003.

• Yan, L., He, Q., Xiao, S., Lu, J. and Chen, H.. Expression of Porcine Interleukin-2 and The Adjuvant Effects on The Gene Deleted Vaccine of Pseudorabies Virus ((TK-/gG-/LacZ+). The 1st Asian Veterinarian Society Congress. KyoYukMunHwaHoeKwan, Seoul, South Korea. 2003.

• Yan, L., Mou, F., and Chen, H.. The Adjuvant Effects of Recombinant pIL-2 on Attenuated Pseudoeabies Virus Vaccine in a Murine Model. The 3rd Annual Conference of Animal Microbiology Division, Chinese Veterinary Medical Association. 2004.

• Yan, L., He, Q., Chen, H., Xiao, S., Wu, M., Lv, J., Han, L. Expression of Porcine Iinterleukin-2 and Porcine Interleukin-6 and their Adjuvant Effects on Gene Deleted Vaccine of Pseudorabies Virus (TK-/gG-/LacZ+). 2004 China Doctoral Candidates’ Academic Forum. 2004.

• Yan, L. Selected prfA* Mutations in Recombinant Attenuated Listeria monocytogenes Strains Augment Expression of Foreign Immunogens and Enhance Vaccine-Elicited Humoral and Cellular Immune Responses. The 12th Annual Conference on Vaccine Research. Apr. 27-29, 2009. Baltimore, MD

REFERENCES: AVAILABLE UPON REQUEST

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