Tim D. Brown

**** *******’s Crossing Dr Grimesland, NC 27837, U.S.A.

Home: 252-***-****

E-Mail: q2jnkn@r.postjobfree.com

Education

B.S./Microbiology

Kansas State University Manhattan, KS

Degree: 5/1999; GPA: 3.364

M.S./Molecular Biology and Biotechnology

Thesis : The glutamate decarboxylase genes, gadBC, are not required for Brucella abortus resistance to low pH or virulence in the mouse model.

East Carolina University Greenville, NC

Degree: 05/2007

Work Experience

Lab Analyst

Greenville Utilities Corporation

Greenville, NC 27834

(7/2010-present)

• Lab analyst in the water treatment plant laboratory

• Responsible for physical chemistry testing of raw, in-process, and finished water

• Responsible for microbiological testing of raw, in-process, and finished water

• Developed assay for rapid identification of bacteria in water using qPCR techniques

Instructor

Miller-Motte College

Greenville, NC 27834

(7/2010-10/2010)

• Microbiology instructor, teaching two Intro to Microbiology courses

Lab Analyst I

DSM Pharmaceuticals, Inc.

Greenville, NC 27834

(9/2009-3/2010)

• Performed testing for microbial contamination of Water For Injection (WFI) and Purified Water (PFW) systems daily.

• Performed Bacterial Endotoxin Tests by the gel-clot method on pharmaceutical in-process and finished product, excipients, and water.

• Performed total organic carbon tests using Siever’s analyzers for cleaning validations.

• Various other duties as assigned, complied with all cGMP requirements and maintained accurate and complete records of my work.

Microbiologist

Metrics, Inc.

Greenville, NC 27834

(2/2008-3/3009)

• Performed water testing of USP quality water systems, microbial limits testing, microbial preparatory testing, antimicrobial effectiveness testing, particulate matter analysis by both microscopic and light obscuration method.

• Maintained records of environmental monitoring, autoclave biological indicator challenges, monthly lab maintainance, etc.

Science Teacher

North Pitt High School

Pitt County Schools

Greenville, NC 27834

• Taught three Earth Science classes daily. Gained invaluable experience working with a diverse population and resolving conflict.

Research Specialist

East Carolina University

Brody School of Medicine (12/00-6/2007)

Dept. of Microbiology and Immunology

• Responsible for overseeing day-to-day operation of both a Biosafety level 2 and Biosafety level 3 laboratory.

• Duties include ordering supplies and maintaining inventory, maintaining records of all bacterial strains, and conducting research.

• Responsible for training all new members of the laboratory.

• Responsible for maintaining compliance with all Federal, State, and Local regulations regarding work with Select Biological Agents.

• Liaison between different departments and groups to ensure successful application and registration to posses and conduct research with select agents

• Responsible for writing scientific research papers and preparing presentations for local, regional, and international meetings

Graduate Assistant

LSU Health Science Center (7/99-9/00)

Shreveport, LA

• Responsible for conducting research into the immunology of malaria infection. Gained experience with a variety of techniques in molecular biology, including RT-PCR.

Student Lab Assistant

Food Animal Health Management Center (5/98-5/99)

Manhattan, KS

• Responsible for general laboratory duties, cleaning glassware, making media, bacterial isolation and cultivation. Gained familiarity with Good Laboratory Practice, aseptic technique, and bacterial cultivation techniques.

Presentations

A Genetic Analysis of the Role of The Brucella abortus HdeA in Acid Resistance and Virulence. Michelle Wright Valderas, John E. Baumgartner, Tim D. Brown*, and R. M. Roop II at the 46th annual Wind River conference on Prokaryotic Biology, Estes Park, CO June 2002.

Do the gadAB and gadC Gene Products Contribute to Stationary Phase Acid Resistance of Brucella abortus? Tim Brown* and R. Martin Roop II at the 46th annual Wind River conference on Prokaryotic Biology, Estes Park, CO June 2002.

Do the gadB and gadC Gene Products Contribute to Stationary Phase Acid Resistance of Brucella abortus? Tim D. Brown, Michelle Wright-Valderas, and R. Martin Roop II. at the Fall 2002 Meeting North Carolina Branch American Society for Microbiology, Shaw University, Raleigh, NC Nov. 2002.

Do the gadB and gadC Gene Products Contribute to Stationary Phase Acid Resistance of Brucella abortus? Tim D. Brown, Michelle Wright-Valderas, and R. Martin Roop II. at the 55th Annual Brucellosis Research Conference, St. Louis, MO November 9-10 2002.

The gadB and gadC gene products do not play a role in acid resistance of Brucella abortus. Tim D. Brown, Michelle Wright-Valderas, and R. Martin Roop II. at the 1st annual Mid-Atlantic Microbial Pathogenesis Meeting, Wintergreen, VA February 3-5 2003.

GABA, GABA, Hey ! : The Glutamate Decarboxylase System in Brucella abortus. Tim Brown, Michelle Wright-Valderas, and R. Martin Roop II, at the 47th annual Wind River conference on Prokaryotic Biology, Estes Park, CO June 2003.

The Glutamate Decarboxylase System in Brucella abortus is not Required for Virulence in the Mouse Model. Tim Brown, Michelle Wright-Valderas, and R. Martin Roop II. at Brucellosis 2003 International Research Conference including the 56th Brucellosis Research Conference September 15-17, 2003 University of Navarra, Pamplona (Spain)

Phenotypic analysis of the alternative sigma factors of Brucella abortus.

Michael Hornback, John Baumgartner, Timothy Brown, Kendra Hitz, and R. Martin Roop II. at 2005 Mid-Atlantic Microbial Pathogenesis Meeting, Wintergreen, VA February 6-8, 2005.

Publications

Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infect Immun. 2004 Aug;72(8):4911-7.

Anderson ES, Paulley JT, Gaines JM, Valderas MW, Martin DW, Menscher E, Brown TD, Burns CS, Roop RM 2nd. The manganese transporter MntH is a critical virulence determinant for Brucella abortus 2308 in experimentally infected mice. Infect Immun. 2009 Aug;77(8):3466-74

Dola Sengupta, Alicia Koblansky, Jennifer Gaines, Tim Brown, A. Phillip West, Dekai Zhang, Tak Nishikawa, Sung-Gyoo Park, R. Martin Roop, II, and Sankar Ghosh.

Subversion of Innate Immune Responses by Brucella through the Targeted Degradation of the TLR Signaling Adapter, MAL. J. Immunol., Jan 2010; 184: 956 - 964.

Honors and awards

Golden Key National Honor Society member

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