Quality Control Development

Sandra Lynn Nelson

***** ****** ***** ****

Loveland, Ohio 45140

513-***-**** (cell)

513-***-**** (home)

pvbt8z@r.postjobfree.com

EMPLOYMENT HISTORY

University of Cincinnati 9 / 2007 – 6 / 2012

Research Scientist

Head of High Throughput and High Content Screening core lab for Drug Discovery Center at the University of Cincinnati.

Evotec Technologies / PerkinElmer 2006 – 2007

Director, High Throughput Screening

Established and run High Throughput and High Content Screening core lab for Drug Discovery group at the University of Cincinnati.

Procter & Gamble 1988 – 2006

1998 – 2006 P&G Pharmaceuticals Discovery, High Throughput Screening (HTS) Section

10 – 19 people

9/2006 closed P&GP Discovery Research, retired from P&GP

1/2004 added responsibility for Compound Repository for P&GP

12/2002 added responsibility for hit-to-lead screening and assay development for P&GP Discovery

4/1999 became Section Head of HTS, responsible for all P&GP HTS

Also leader for drug discovery therapeutic project

in collaboration with a biotech

1993 – 1998 P&G OTC Health Care Product Development

Responsible for biochemistry aspects of bitterness inhibition flavor projects and projects to decrease flavor negatives of metallic compounds in products.

Responsible for breath malodor research project for dentifrice products

Responsible for model development of oral care product interactions with oral cavity to understand and manipulate all aspects of flavor

Member of teams that produced several new toothpaste products

1988 – 1993 P&G Health Care Discovery, Analytical Section

Responsibility for immuno-analytical and analytical biochemistry measurements for the Division doing research for pharmaceutical and OTC products

Conducted in vitro assays supporting Discovery projects and supporting clinical

trials

National Animal Disease Center, USDA 1984 - 1988

Research Biochemist

Determined Vitamin D steroid receptor relationship to milk cattle metabolism

Studied bovine respiratory disease, focusing on response to Pasteurella haemolytica. Developed subunit vaccine.

Ames Laboratory, USDOE 1980 - 1984

Staff Chemist

Developed biochemical assays to detect environmental carcinogens using protein chemistry, fluorescence, cell culture, chromatography and spectroscopy.

EDUCATION

B.S. May 1975 Biochemistry Iowa State University

Ph.D. March 1980 Biochemistry University of North Carolina

SCIENTIFIC ACTIVITIES

2007 – 2010 elected member Board of Directors for the Society for Biomolecular Screening

2000 - present reviewer for the Journal of Biomolecular Screening

2009 - present ad hoc reviewer for Drug Discovery Today

2003 – 2008 editorial board member for the Journal of Biomolecular Screening

2009 – present member NIH grant review study section for Roadmap Assay Development for High Throughput Molecular Screening (R21)

February 19-20, 2009

June 11 -12, 2009

January 28 – 29, 2010

November 4 – 5, 2010

March 2 – 3, 2011

2009 – present member NIH grant review study section for Roadmap HTS Assay for MLPCN (R03)

July 30, 2009

March 18, 2010

August 9, 2010

November 12, 2010

March 15, 2011

July 20 – 21, 2011

2008 - 2010 Expert reviewer for North Carolina Biotechnology Center Grants

2005 reviewer for Drug Discovery

2001 – 2006 member Molecular Biotechnology HTS Graduate Program Oversight Committee at Western Michigan University

2003 Organized first Midwest Chapter Laboratory Robotics Information Group (LRIG) meeting in Cincinnati in March

April 10 - 12, 2000 member of Special Emphasis Panel to review 12 grant applications in response to RFA 99-010, “National Cooperative Drug Discovery Groups” for the National Cancer Institute

PATENTS

Sandra L. Nelson. Inhibiting Undesirable Taste in Oral Compositions. United States Patent No. 5,766,622. Filed August 14, 1996. Awarded June 16, 1998.

Sandra L. Nelson. Inhibiting Undesirable Taste in Oral Compositions. European Patent No. 97937072.3-2107. Filed July 28, 1997. Awarded June 8, 1999.

J.C. Hayes, G.L. Walden and S.L. Nelson. Use of phosphatase inhibitors, e.g. Vanillylmonophophate, In Taste Cells to Inhibit Undesirable Taste - Used in Foods, Drinks, Pharmaceuticals, Toiletries and Dental Compositions. European Patent # 845217. Filed November 11, 1997. Published June 3, 1998.

PUBLICATIONS

S.N. Walters and P. Morell. Effects of Altered Thyroid States on Myelinogenesis. J. Neurochem. 36:1792-1801. (1981)

S.N. Walters, A.P. D'Silva and V.A. Fassel. Immobilized Enzyme System for the Conversion of Benzo[a]pyrene to Fluorescent Metabolites. Anal. Chem. 54:2571-2576. (1983)

G.D. Renkes, S.N. Walters, S.S. Woo, M.K. Iles, A.P. D'Silva and V.A. Fassel. Determination of Aromatic Hydrocarbons in Particulate Samples by High-Temperature Extraction and Shpol'skii Spectroscopy. Anal. Chem. 55:2229-2231. (1983)

S.N. Walters, T.A. Reinhardt, M.A. Dominick, R.L. Horst and E.T. Littledyke. Intracellular Location of Unoccupied 1.25-Dihydroxyvitamin D Receptors: A Nuclear-Cytoplasmic Equilibrium. Archiv. Biochem. Biophys. 246:366-373. (1986)

S.L. Nelson and G.H. Frank. Bovine Serum and Nasal Secretion Immunoglobulins Against Pasteurella haemolytica Serotype 1 Antigens. Am. J. Vet. Res. 50:1244-1249. (1989)

S.L. Nelson and G.H. Frank. Purification and Characterization of a 94 kD Pasteurella haemolytica Antigen. Vet. Microbiol. 21:57-66. (1989)

G.H. Frank, S.L. Nelson and R.E. Briggs. Infection of the Middle Nasal Meatus of Calves with Pasteurella haemolytica, Serotype 1. Am. J. Vet. Res. 21:1297-1301. (1989)

S.L. Nelson, B.A. Hynd and H.M. Pickrum. Automated Enzyme Immunoassay to Measure Prostaglandin E2 in Gingival Crevicular Fluid. J. Periodont. Res. 27: 143-148. (1992)

S.L. Nelson and B.A. Hynd. ELISA Kits Measuring Human IgG Subclasses - Cross-Reactivity Against IgG3 in an IgG1 ELISA. Clinical Chem. 39: 1919. (1993)

S.L. Nelson, B.A. Hynd and M.W. Seibel. An Automated Sensitive ELISA to Measure IgE Produced by Cultured Human Lymphocytes. Laboratory Robotics and Automation. 7: 25-33. (1995)

M.K. Jeffcoat, M.S. Reddy, S. Haigh, W. Buchanan, M.J. Doyle, M.P. Meredith, S.L. Nelson, M.B. Goodale and K.R. Wehmeyer. Comparison of Topical Ketorolac, Systemic Flurbiprofen and Placebo for the Inhibition of Bone Loss in Adult Periodontitis. J. Periodont. Res. 66: 329-338. (1995)

S.L. Nelson and J.D. Sanregret. Response of Pigs to Bitter-Tasting Compounds. Chem. Senses 22: 129 - 132 (1997).

G.F. Davis, T.R. Downs, J.A. Farmer, C.R. Pierson, J.T. Roesgen, E.J. Cabrera, and S.L. Nelson. Comparison of High Throughput Screening Technologies for Luminescence Cell-Based Reporter Screens. J. Biomolecular Screening 7: 67 – 78. (2002)

S. Roychoudhury, K.M. Makin, T.L. Twinem, D.T. Stanton, S.L. Nelson, and C.E. Catrenich. Development and Use of a High-Throughput Bacterial DNA Gyrase Assay to Identify Mammalian Topoisomerase II Inhibitors with Whole-Cell Anticancer Activity. J. Biomolecular Screening 8 (2): 157 – 163. (2003)

B.A. Kozikowski, T.M. Burt, D.A. Tirey, L.E. Williams, B.R. Kuzmak, D.T. Stanton, K.L. Morand, and S.L. Nelson. The Effect of Room-Temperature Storage on the Stability of Compounds in DMSO. J. Biomolecular Screening 8 (2): 205 – 209. (2003)

B.A. Kozikowski, T.M. Burt, D.A. Tirey, L.E. Williams, B.R. Kuzmak, D.T. Stanton, K.L. Morand, and S.L. Nelson. The Effect of Freeze/Thaw Cycles on the Stability of Compounds in DMSO. J. Biomolecular Screening 8 (2): 210 – 215. (2003)

S.L. Nelson. Academic HTS: diverse portraits. Drug Discov. Today: Technol. doi:10.1016/j.ddtec.2009.01.001 (2009)

Jennifer F. Barger, Catherine A. Gallo, Kyle A. Torni, Lisa Merk, William L. Seibel, Sandra Nelson and David R. Plas. Identification of Akt-selective cytotoxic compounds that enhance cytotoxic responses to rapamycin. Cancer Biology & Therapy 10(12): 1256 – 1261. (2010)

Matthew Wood, Melissa Rawe, Gunnar Johansson, Shu Pang, Ryan Soderquist, Ami Patel, Sandra Nelson, William Seibel, Nancy Ratner, and Yolanda Sanchez. Discovery of a small molecule targeting IRA2 deletion in budding yeast and neurofobromin loss in malignant peripheral nerve sheath tumor cells. Molecular Cancer Therapeutics, 10:1740-1750. (2011)

Automated Analysis and Classification of Infected Macrophages using Bright-field Amplitude contrast Data. Umesh Adiga, Brian L Bell, Larissa Ponomareva, Debbie Taylor, Sandra Nelson, Thomas J. Lamkin Jounal of Biomolecular Screening, in press. (2012)

Automatic Mapping of Cell Phenotype to Infection Stages. Umesh Adiga, Brian L Bell, Larissa Ponomareva, Debbie Taylor, Roland Saldanha, Sandra Nelson, Thomas J. Lamkin. IEEE Trans. on Biomedical Engineering, in press (2012)

INVITED PRESENTATIONS

"The Steroid Receptor for Vitamin D." Chemistry Department Seminar. University of Northern Iowa. October 25, 1984.

"Antibody-Based Techniques for Analytical Chemistry." Seventeenth Annual Analytical Symposium. Cincinnati, Ohio. April, 1989.

"Use of in vivo Saliva Enzymes to Deliver Unique Flavor Sensations." Procter & Gamble Life Sciences & Biotechnology Symposium. Cincinnati, Ohio. February, 1996.

“Changing Compound Storage Conditions to Improve Compound Integrity.” MipTec 2002. Basel, Switzerland. May, 2002

“Finding the Optimum Level of Quality Control in the Practical World of HTS,” Keynote Presentation. High-Throughput Screening for Drug Discovery. Boston, Massachusetts. March, 2004.

“Most Efficient Use of High Content Screening to Find Anti-Microbial Agents.” PerkinElmer Users Group. High-Content Analysis East. Boston, Massachusetts. September 2009.

CONTRIBUTED ORAL PRESENTATIONS

S.N. Walters, A.P. D'Silva and V.A. Fassel. Use of Immobilized Aryl Hydrocarbon Hydroxylase to Assay for Benzo[a]pyrene in Environmental Samples. Sixth International Symposium on Polynuclear Aromatic Hydrocarbons. Columbus, Ohio. (October, 1981)

S.L. Nelson and G.H. Frank. Purification of an Antigenic Glycoprotein of Pasteurella haemolytica. Annual Meeting of the North Central Branch of the American Society of Microbiology. Iowa City, Iowa. (November, 1987)

S.L. Nelson, Kenneth Morand, Barbara Kozikowski, Debra Tirey, Tom Burt, Lisa Williams, Barbara Kuzmak, and David Stanton. Stability of Compounds Stored in DMSO. Seventh Annual Conference and Exhibition of the Society for Biomolecular Screening. Baltimore, Maryland. (September, 2001)

CONTRIBUTED POSTER PRESENTATIONS

S.N. Walters and P. Morell. Effect of Altered Thyroid States on Myelin Proteins during Development. Trans. Am. Soc. Neurochem. 10:180. Charleston, South Carolina. (March, 1979)

S.N. Walters, A.P. D'Silva and V.A. Fassel. Immobilized Aryl Hydrocarbon Hydroxylase for Determination of Benzo[a]pyrene in Environmental Samples. Fed. Proc. 41:1296. New Orleans, Louisiana. (April, 1982)

S.N. Walters, M. Dominick, T.A. Reinhardt, R.L. Horst and E.T. Littledyke. Localization of Unoccupied 1,25-(OH)2D3 Receptors in Both the Cytoplasm and Nucleus of Two Kidney Cell Lines. Department of Biochemistry and Biophysics Silver Jubilee Symposium. Ames, Iowa. (June, 1985)

R.E. Briggs, M.E. Kehrli, S.N. Walters and G.H. Frank. In Vivo Effects of IBR Virus and PI-3 Virus on Bovine PMN Function. Proc. First International Veterinary Immunology Symposium. 1:154. Guelph, Ontario, Canada. (July, 1986)

R.E. Briggs, S.N. Walters and G.H. Frank. Effect of Pasteurella haemolytica Leukotoxin on Functions of Polymorphonuclear Neutrophils from Healthy and Infectious Bovine Rhinotracheitis Virus-Infected Calves. The 67th Conference of Research Workers in Animal Disease. Chicago, Illinois. (November, 1986)

S.N. Walters and G.H. Frank. Antibody Responses to Antigenic Components of Pasteurella haemolytica Serotype 1 in Cattle. Proc. International Symposium on Virulence Mechanisms of Veterinary Bacterial Pathogens. 1:38. Ames, Iowa. (June, 1987)

G.H. Frank, R.E. Briggs and S.L. Nelson. Colonization of the Nasal Passages of Calves with Pasteurella haemolytica Serotype 1. The 68th Conference of Research Workers in Animal Disease. Chicago, Illinois. (November, 1987)

S.L. Nelson and G.H. Frank. Bovine Antibody Against a Pasteurella haemolytica Glycoprotein Associated with Resistance to Nasal Colonization. FASEB J. 2:A1255. Las Vegas, Nevada. (May, 1988)

R.E. Briggs, S.L. Nelson and G.H. Frank. Bovine Leukocyte Elastase and N-acetyl-glucosaminidase in Nasal Secretions of Calves Undergoing Respiratory IBR Virus Infection. The 69th Conference of Research Workers in Animal Disease. Chicago, Illinois. (November, 1988)

S.L. Nelson, J.K. Carter and B.A. Hynd. Purification of Ferret Albumin. Ninth International Symposium on HPLC of Proteins, Peptides and Polynucleotides. Philadelphia, Pennsylvania. (November, 1989)

S.L. Nelson. ELISA to Measure Antibody Against Campylobacter pylori in Human Sera or Plasma. Procter & Gamble's Seventeenth Annual Analytical Symposium. Cincinnati, Ohio. (April, 1989)

S.L. Nelson and B.A. Hynd. ELISA Automation: Immunochemistry Untouched by Human Hands. Procter & Gamble's Eighteenth Annual Analytical Symposium. Cincinnati, Ohio (April, 1990)

S.L. Nelson and B.A. Hynd. Biochemical Techniques to Characterize and Purify Proteins. Procter & Gamble's Nineteenth Annual Analytical Symposium. Cincinnati, Ohio. (May, 1991)

J.R. Kaczvinsky, K.G. Kronholm, C.L. MacBrair, C.A. Marcott, A.E. Dowrey, D.E. Burton, D.L. Bailey, LC. Perkins, M. Mikolajewske, S.L. Nelson, B.A. Hynd. An Example of the Analytical Approach: The SK-II Story. Procter & Gamble's Twentieth Annual Analytical Symposium. Cincinnati, Ohio. (May, 1992).

M.K. Jeffcoat, M.S. Reddy, W. Buchanan, M.B. Goodale, M.J. Doyle, M.P. Meredith, S.L. Nelson, and K.R. Wehmeyer. Ketorolac Tromethamine Rinse Inhibits the Progression of Bone Loss in Adult Periodontitis. The 1993 International Association for Dental Research Meeting. Chicago, Illinois. (March, 1993).

M.A. Smith, W. Buchanan, M.J. Doyle, G.R. Kelm, M.P. Meredith, S.L. Nelson, T.T. Charles, K.R. Wehmeyer, J.G. Collins, D.L. Boyd and S. Offenbacher. Inhibition of PGE2 with Ketorolac Rinses. The 1993 International Association for Dental Research Meeting. Chicago, Illinois. (March, 1993).

S.L. Nelson, M.W. Seibel and B.A. Hynd. Sensitive ELISA to Measure IgE Produced by Cultured Human Lymphocytes. Procter & Gamble's Twenty-first Annual Analytical Symposium. Cincinnati, Ohio. (May 1993).

M.W. Seibel, S.L. Nelson and B.A. Hynd. ELISA Assays to Measure Total IgE in Murine Cell Culture and Serum Matrixes. Procter & Gamble's Twenty-first Annual Analytical Symposium. Cincinnati, Ohio. (May 1993).

B.A. Hynd and S.L. Nelson. Robotic Assay Quantifying Microgram Amounts of Protein. Procter & Gamble's Twenty-first Annual Analytical Symposium. Cincinnati, Ohio. (May 1993).

S.L. Nelson, M.W. Seibel and B.A. Hynd. Sensitive Enzyme Immunoassay to Measure IgE Produced by Cultured Human Lymphocytes. Journal of Immunology 150: 150A. Denver, Colorado. (May 1993)

J.D. Sanregret and S. L. Nelson. Automated Enzyme Kinetics Assay of Tyrosine Phosphatase to Understand Flavor Phosphates' Mechanisms to Decrease Negative Flavor Attributes. Procter & Gamble Life Sciences & Biotechnology Symposium. Cincinnati, Ohio. (February 1996).

G.E. Ficken-Clarke and S.L. Nelson. Consumer Response to Crest Gum Care: Saliva Protein Measurements and Sensory Determination of Astringency. Procter & Gamble Life Sciences & Biotechnology Symposium. Cincinnati, Ohio. (February 1996).

G.E. Ficken-Clarke and S.L. Nelson. Consumer Response to Crest Gum Care: Saliva Protein Measurements and Sensory Determination of Astringency. Procter & Gamble 1996 Sensory Symposium. Cincinnati, Ohio. (March 1996)

S.L. Nelson, G.E. Ficken-Clarke and J.D. Sanregret. Oral Astringency Caused by Stannous Ions: Sensory and Biochemical Correlations. International Symposium on Olfaction and Taste XII. San Diego, California. (July 1997)

T. Ogura, S.L. Nelson and S.C. Kinnamon. Intracellular Ca2+ responses to dextromethorphan in isolated taste cells of mudpuppy. International Symposium on Olfaction and Taste XII. San Diego, California. (July 1997)

S.L. Nelson, J.D. Sanregret, and G.E. Ficken-Clarke. Sensory and Biochemical Correlations of Astringency: Application to Crest. Procter & Gamble R&D Week Flavor Symposium. (March 1998)

S.L. Nelson, C. Sheets, C. Davis, L. Whiteley, J.D. Sanregret and D. McMillan. Model Development to Predict Oral Irritation Caused by Dentifrice. Procter & Gamble R&D Week Life Sciences Symposium. (March 1998)

C. Sheets, C. Davis, J. Stapler, G.E. Ficken-Clarke, S.L. Nelson, D. McMillan and B. Olin. Novel Dentifrice Formulation Effects on Oral Sensory Discomfort. Procter & Gamble R&D Week Life Sciences Symposium. (March 1998)

G.F. Davis, J.A. Farmer and S.L. Nelson. Cell-based Luciferase Reporter Gene Assay on a Beckman-Sagian Robotic Platform Designed for High Efficiency and Throughput. IBC’s Second International Symposium: Real Case Studies on Developing Effective Assays. Philadelphia, Pennsylvania. (October 1998)

Gregory F. Davis, Julie A. Farmer, Lee Garbutt, Thomas R. Downs, Edelberto J. Cabrera and Sandra L. Nelson. Cell-Based Luciferase Reporter Gene Assay on a Beckman-Sagian Robotic Platform Designed for High Efficiency and Throughput. P&G’s 2nd Annual Fall Life Sciences/Biotechnology Poster Symposium. Cincinnati, Ohio. (October 1998)

G.F. Davis, J.A. Farmer, C.R. Hicks, N.J. Izzo, T.L. Carrick and S.L. Nelson. Comparison of Transcription and Translation HTS Screens for the Same Bioactive Protein. Society for Biomolecular Screening 5th Annual Conference and Exhibition. Edinburgh, Scotland, UK. (September 1999)

J.R. Davis, J.W. O’Neill, M.J. Gopsill, D.L. Garbutt and S.L. Nelson. Utilizing Beckman-Sagian SAMI NT for Throughput Improvement in a Receptor Binding Assay. Society for Biomolecular Screening 5th Annual Conference and Exhibition. Edinburgh, Scotland, UK. (September 1999)

Sandra L. Nelson, Nancy Ratner, Gunnar Johansson, Yolanda Sanchez, Markus Wolf, Lisa Merk, Bill Seibel, Stacey Frazier. High Throughput Screening to Seek New Therapeutics and New Research Tools for Neurofibromatosis. Ohio Valley Affiliates for Life Sciences Conference. Cincinnati Ohio. (March 2007)

Sandra L. Nelson, Nancy Ratner, Gunnar Johansson, Yolanda Sanchez, Markus Wolf, Lisa Merk, William Seibel, Stacey Frazier. Seeking New Therapeutic Agents and New Chemical Probes for the Genetic Disease Neurofibromatosis using HTS. Society for Biomolecular Sciences 13th Annual Conference. Montreal, Canada, (April 2007)

Sandra L. Nelson, Shadi Abu-Baker, Mustapha Bahassi, Lisa Merk, Markus Wolf, D.J. Ferguson, Yaqin Li, Maria Diaz-Meco, Jorge Moscat, Peter Stambrook. Three Kinase Screening Assays Using a Generic ADP Detection Method. Society for Biomolecular Sciences 14th Annual Conference. St. Louis, Missouri, (April 2008)

Sandra Nelson, Lisa Merk, Jennifer F. Barger and David Plas. Academic Drug Discovery: Targeting Cancer Apoptosis Resistance with a Cell-Based Screen. Society for Biomolecular Sciences 15th Annual Conference. Lille, France, (April 2009)

Hong Tang, Sandra Nelson, Lisa Merk, William Seibel, I-Chang Wang, Tatiana Kalin, Vladimir Kalinichenko. High-Content Screening for Small Molecule Inhibitors of Forkhead Box M1 (FoxM1). Society for Laboratory Automation and Screening Annual Conference. San Diego, California, (February 2012)

RECENT ACCOMPLISHMENTS

The University of Cincinnati HTS lab completed 15 screens in 2011. During the 6 years of my leadership the HTS lab completed over 70 screens. The HTS lab provides screening for a fee and has collaborated with scientists from the University of Cincinnati Faculty, Cincinnati Children’s Hospital Medical Center, other US Universities (3 in other states), 4 Companies (1 in Europe), and the US Military. The lab has included one to three scientists reporting to Dr. Nelson over this period.

AWARDS

2005 P&G award for management support of safety

2000 Special Award to HTS team member from Beckman Coulter and Perkin-Elmer for contributions to HTS and the Society for Biomolecular Screening

2000, 2001 SBS / IGEN Poster Award

to HTS team poster, for one of ten best posters, based on scientific content, innovative approaches and new information, at Society of Biomolecular Screening meeting

1999 SBS / IGEN Poster Award

author of one of ten best posters at SBS meeting

1999 1st place poster award at LabAutomation 1999 to HTS team

2000, 1995 Marquis Who's Who in the World

1997, 1996 Marquis Who's Who in Science and Engineering

1994, 1993 Marquis Who's Who in Science and Engineering

1990, 1991 P&G awards for team achievement

1975 B.S. with Honors, Honors Program, Phi Beta Kappa, Phi Lambda Upsilon,

Iota Sigma Pi

1971-1975 National Merit Scholar

1975 ISU Department of Biochemistry and Biophysics Scholarship Award

1974 ISU Undergraduate Honors Program Research Grant Award

1971 Iowa State Scholar

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