Microbiologist/ Molecular Biologist / QA/ QC

________________________________________VAIBHAV R. DESHPANDE

Mobile: 097******** / 076******** Phone: 025*-******* E-Mail: ******************@*****.***

________________________________________

Seeking assignments in the domain of Research & Development/ Quality Assurance & Control with a growth oriented organisation

AN OVERVIEW

Doctor of Philosophy (Ph.D.) in Microbiology from Seth G. S. Medical College & K.E.M. Hospital, Mumbai, Mumbai University (Pursuing – Thesis submitted); Masters in Science - Biotechnology from Department of Biotechnology, Birla College of Arts, Science & Commerce, Kalyan, Mumbai University in 2005.

Abilities in handling microbiology operations as well as implementing various quality management initiatives.

Capable of managing the spectrum of activities spanning testing, quality control and coordination with cross-functional departments.

Quick learner & self-directed; consistent updating self with the emerging trends in the industry.

An efficient key player in challenging & creative environments with excellent capacity to adapt to new technologies and skills.

INTERNSHIP

Ph.D. Scholar Since May’06

Research Supervisor: Dr. M G Karmarkar, Professor, Research laboratory, Department of Microbiology, Seth GS Medical College & KEM Hospital, Parel, Mumbai-12.

Institute: Department of Microbiology, Seth GS Medical College & KEM Hospital, Parel, Mumbai-12.

‘Field Assistant cum Data Entry operator’ on ICMR RF / RHD Registry project Aug’07 to May’10

Job responsibilities involved, to work with Lab Technician in Microbiological & Molecular Biology aspects of Project this includes collection of throat swabs, Isolation, Identification, grouping & preservation of β-haemolytic streptococci; emm typing; Quantitative ASO determination of all suspected cases and Preparation of Active surveillance (School survey) master registry which includes details of School children screened with the microbiological findings, Anthropometric measurements, blood pressure records, clinical findings & 2D ECHO findings (This data was collected by Medical officer & other field assistants); Preparation of master registry of Passive surveillance, which include records of old & new cases of RF/ RHD from our hospital as well as from other sub-registries; Analysis of data.

‘Data Entry Operator & Analyst’ in VD Data Services May’05 to Apr’06

Responsibilities: Dossier preparation of different countries.

EDUCATION & CREDENTIALS

Thesis Doctor of Philosophy (Ph.D.) in Microbiology from Seth G. S. Medical College & K.E.M. Hospital, Mumbai,

Submitted Mumbai University

Title: Study of Enterococcus with Special Emphasis on Characterization of Vancomycin Resistance, Lateral Transfer of Drug Resistance & Plasmid Profiles

2005 Masters in Science (M.Sc.) - Biotechnology from Department of Biotechnology, Birla College of Arts, Science & Commerce, Kalyan, Mumbai University. Secured 1st Division

2003 Bachelor of Science - Microbiology / Biotechnology from Birla College of Arts, Science & Commerce, Kalyan, Mumbai University. Secured 1st Division

Additional Qualifications:

PG Diploma in Clinico-Regulatory & Patents from Pharmaceutical Experts Association (PEXA) in collaboration with S. P. Mandali, Pune at Ruia College, Matunga in 2005.

PG Diploma in Bioinformatics from Rishibiotech, Thane in 2004.

Diploma in Hardware and Networking from Microtech Computer Institute, Thane.

IT Forte:

Operating Systems : Windows (95/98/2000/XP) and DOS

Application Packages : MS Office (Word, Excel & PowerPoint) and Front Page

Languages : C, C++, Perl, Java

Database : SQL

Web : HTML

RESEARCH EXPERIENCE

Doctor of Philosophy Project:

Title of Thesis: Study of Enterococcus with Special Emphasis on Characterization of Vancomycin Resistance, Lateral Transfer of Drug Resistance & Plasmid Profiles

Other Projects: (KEM)

Population structure of Streptococcus dysgalactiae subspecies Equisimilis from Mumbai school children.

Study on the kinetics of human immune response to Streptococcal c5a Peptidase and its correlation with anti -Deoxyribonuclease B (ADNase B) and Anti-Streptolysin O (ASO) titers.

Study of β hemolytic streptococcal isolates from skin infections.

Project: Anti -bacterial Activity of Indian Medicinal Plants against Clinical Isolates of Enterococci.

Indian Council of Medical Research (ICMR) project on “Establishment of a Rheumatic Fever (RF) and Rheumatic Heart Disease (RHD) registry in Mumbai (Satellite Centre).”

In vitro Study on Efficacy of Ceftriaxone in Combination with Two β-lactamase Inhibitors (Tazobactam & Sulbactam) against gram-Negative Clinical Isolates.

Epidemiological study of Group B Streptococci in Mumbai.

Master’s Dissertation Project:

“In vitro Culture Studies of Hibiscus Rosa Sinensis”.

Bachelor’s Dissertation Project:

“Single Cell Protein Production”.

TECHNICAL TRAINING

Shreeji Computerized Path. Lab, Kalyan, Thane: ‘Techniques of Clinical Pathology’ for 20 days.

Dhanvantari Hospital & IC.C.U., Thane: ‘Techniques of Clinical Pathology’ for 20 days.

‘Bombay Veterinary College, Mumbai: ‘Techniques in Biotechnology’ for 15 days.

‘MITCON, Thane’ an Industrial & Technical Consultants Govt. of Maharashtra, India: ‘Medicinal Plants & Processing’ from 25th Feb’07-1st Apr’07.

Anchrom Enterprises (I) P. Ltd., Mumbai (CAMAG Switzerland recognized Applications Research Laboratory in Mumbai) for 3 days on ‘High Performance Thin Layer Chromatography’.

WORKSHOPS

Advanced workshop cum Hands on Training on ‘High Performance Liquid Chromatography (HPLC)’ organized by Dr. P.S. Ramanathan Advanced Instrumentation Centre (PSRAIC), Ruia College, Matunga, Mumbai – 400 019 in colloboration with Shimadzu Analytical (India) Pvt. Ltd. held on 5-6 January 2012.

Advanced workshop cum Hands on Training on ‘Gas Chromatography (GC) an Gas Chromatography- Mass Spectroscopy (GC-MS)’ organized by Dr. P.S. Ramanathan Advanced Instrumentation Centre (PSRAIC), Ruia College, Matunga, Mumbai – 400 019 in colloboration with Shimadzu Analytical (India) Pvt. Ltd. held on 2-4 January 2012.

Workshop cum Training Programme on ‘Spectrophotometry’, conducted by Dr.P.S.Ramanathan Advanced Instrumentation Centre (PSRAIC), Chemistry Dept., Ramnarain Ruia College, Mumbai held on 21-26 November 2011.

Workshop cum Training Programme on ‘Structural Bioinformatics’, organised by Bioinformatics Centre Birla Institute of Scientific Research, Jaipur held on 2-4 September 2004.

‘Modern Techniques in Biotechnology’, organised by Biotechnology Department, Institute of Science, Mumbai- 400032 in collaboration with Bangalore Genei Pvt. Ltd, Bangalore during December 2003.

CONFERENCE / SYMPOSIUM / SEMINARS

Co-author of papers “In Contrast to Pharyngitis, Skin infections are associated with GAS more commonly than SDSE in Mumbai Population” and “Population structure of Streptococcus dysgalactiae subspecies equisimilis from Mumbai school children” presented at XVIIIth Lancefield International Symposium on Streptococci and Streptococcal Diseases at Palermo, Italy on 4th -8th September 2011.

Presented a paper on “Anti -bacterial Activity of Indian Medicinal Plants against Clinical Isolates of Enterococci” & Co-author of “Epidemiological study of Group B Streptococci in Mumbai” at XVIIth Lancefield International Symposium on Streptococci and Streptococcal Diseases at Porto Heli, Greece on 22nd-26th June 2008.

7th National Conference of ‘Society for Indian Human and Animal Mycology (SIHAM)’ organized by Department of Microbiology, Seth G.S. Medical College & K.E.M. Hospital, Mumbai on 4th-6th February 2008.

Seminar on ‘Recent Trends in Diagnostic Techniques’, organised by Department of Microbiology, Jai Hind College, Mumbai- 400020 held on 15 & 16 March 2007.

‘International Seminar on Bioinformatics (INCOB)’ organized by Department of Biotechnology, Govt. of India at New Delhi on 18th-20th December 2006.

Seminar on ‘Animal Cell Culture – Recent Trends & Application’ organized by Vivekananda Education Society’s College, Chembur, Mumbai on 27th November 2004.

National seminar on ‘Advances in Microbiology: A Boon to Mankind’ organized by Birla College, Thane held on 2004.

Presented a poster on ‘Bioterrorism’ in Symbiotek (GeneRations’02)’ organised by Biotechnology Centre, Indian Institute of Technology (IIT), Mumbai.

PUBLICATIONS

Manuscripts Under preparation:

Prevalence of multidrug resistant Enterococci in a tertiary health care centre of Mumbai, India.

Characterization of Glycopeptide Resistant Enterococci from a tertiary health care center of Mumbai, India.

Virulence factors of Enterococcus faecium & Enterococcus faecalis strains isolated from clinical sources.

Anti-bacterial activity of Indian Medicinal Plants against clinical isolates of Enterococci.

NCBI Nucleotide Sequence Accession Numbers:

Accession Numbers Gene name

JN207928-33 vanA gene of Enterococcus spp.

JF411077-81 vanB gene of Enterococcus spp.

OTHER ACHIEVEMENTS

Member of Organizing Committee of a National Seminar on “Advances in Microbiology: A Boon to Mankind” organised by Birla College, Kalyan & “SIHAM 2008” organised by Seth GS Medical College & KEM Hospital, Mumbai.

Amongst the 500 Indian Research Scholars to participate in ‘Interactive Meet with European & Indian Science Icons’ organized by Department of Science & Technology, Government of India at DST, New Delhi during 8th February 2007.

KNOWLEDGE PURVIEW

Microbiological Skills.

Immobilization.

Microbial Enzyme Assays.

Phage Assays.

Microbial Degradation Studies.

Microbial Extracellular Polysaccharides (Biofilms).

Pigment Characterization.

Microscopy.

Media & Biochemical Preparation.

Antibiotic Sensitivity Assay and Culture Preservation.

Restriction Mapping.

Study of Mutation & Mutation Analysis.

Agarose Gel Electrophoresis.

PCR & Multiplex PCR.

SDS- PAGE.

Western Blotting.

Analysis of Bacterial Proteins.

Thin Layer Chromatography.

Column Chromatography.

Gel Filtration Chromatography.

Dialysis.

Ion-exchange Chromatography.

UV-VIS Spectroscopy.

Atomic Absorption Spectroscopy

FTIR

Gas Chromatography

Gas Chromatography-Mass Spectroscopy

High Performance liquid Chromatography

Thin Layer Chromatography.

High Performance Thin Layer Chromatography.

Isolation & Characterization of Bacteria specifically Human Pathogens.

Microbiological & Biochemical Techniques involved in Industrial Microbiology, Aerobiology, Water & Soil Microbiology.

Identification and Culturing of Fungal Isolates, Micrometry, Isolation of Bioactive Compounds from Fungi & Bacteria.

Diagnostic Tests like ELISA (qualitative & quantitative), Widal, Western Blot Assay, etc.

Extraction of Plasmid DNA & Genomic DNA, RNA from Bacterial Cells & Plants.

Transformation, Conjugation and Screening Recombinants.

Documentation of Gels and Troubleshooting, Sequencing, Analysis of Sequenced DNA.

Quantitative & Qualitative Estimation Assay of Bimolecules.

Plant Tissue Culture & Animal Cell Culture.

Herbal Extract Preparation in vitro efficacy testing of Herbal Extracts against Pathogens.

Nucleic Acid & Protein Analysis.

Homology Search Tools.

Bioinformatics Software - ClustalW, ClustalX, Oligo, PHYLIP, Tree-View, Gene Tool and ORF finder.

PERSONAL PROFILE

Date of Birth: 15th July 1983

Permanent Address: 202, Guru Ashsih, Karve X Road, Vishnunagar, Dombivli (West) 421202 Maharashtra

Present Address: Same as above

Passport Details: No. F5315533 (Expiry: 24’10’15)

Linguistic Abilities: English, Marathi, Hindi

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