Scientist

RONGXIU LI

**** ** ******* ** **** Apt. ***

Parsippany, NJ 07054

Phone: 412-***-****

Cell: 973-***-****

E-Mail: *****@*******.***

Professional Overview

Multi-task SCIENTIST, with 4 years of experience in: Development and performance of reliable ELISA for Pharmacokinetic Study. Evaluation of antibodies anti-tumor effect using cell-based assays. Generation of recombinant antibodies using mammalian cell expression system. Generation of neutralizing monoclonal mouse anti-human antibodies by hybridoma technology. Characterization and chimerization of the neutralizing antibodies. Task-oriented, self trouble-shoot individual with excellent interpersonal / communication skills. Permanent resident of the US.

Core Qualifications

Bacteria, Yeast and Mammalian Cell Cultivation

ELISA

Pharmacokinetic Study

Confocal Microscopy, Fluorescence Microscopy

Colony Assay

Western Blot

Immunofluorescence Cell Staining

Flow Cytometry

Polyacrylamide & Agarose Gel Electrophoresis

PCR / Real-Time PCR

Molecular Cloning

DNA Sequencing

DNA, RNA, Protein extraction and Purification

Transfection

Two-hybrid Assay

Animal Handling

Immunization

Hybridoma Production

Antibody Production and characterization

Antibody Expression

Antibody Chimerization

Chromatography

Experience

2008

Immunomedics. Inc Morris, NJ

Develop and perform reliable ELISA for evaluation of antibodies (to be used in both pre-clinical and clinical) serum stability and clearance.

Generate targeted gene stable expression cell line for evaluation of ADCC and CDC.

Use cell-based assays (e.g.

flow cytometry, western blot, colony assay) to evaluate the anti-tumor effect of antibodies, and investigate the mechanism of the effect.

Generate recombinant antibody producing plasmid DNA construct for mammalian cell expression.

Perform transfection.

Develop reliable assays for screening antibody producing clones and select high producer.

Purify and characterize antibodies.

Design immunization schedule, prepare immunogen and conduct immunization.

Develop reliable assays for screening best immunized animal for fusions and for screening neutralizing antibody producing hybridomas.

Develop reliable assays for characterization of the neutralizing monoclonal antibodies.

Identify VH and VL domains of the mouse antibodies and generate chimeric and humaniz.

2005 to 2008

University of Pittsburgh School of Medicine, Hil Pittsburgh, PA

POSTDOC ASSOCIATE

Investigate novel agents anti-tumor effect.

Develop assays for evaluation synergistic effect of the combination of the novel agents and conventional chemotherapy on head and neck cancers.

Establish reliable methodologies for analyzing the synergistic effect of the combination of novel agents and conventional chemotherapy.

Conduct experiments for investigation the mechanism of the synergism.

Perform experiments for evaluation apoptotic signaling transduction pathways.

August 2003 to October 2004

University of Florida College of Medicine Lab of Gainesville, FL

GRADUATE RESEARCH ASSISTANT

Investigate anti-inflammatory ability of Doxycycline on isolated human endometrial glandular epithelial and.

stromal cells and a human endometrial surface epithelial cell line cultured under defined conditions.

April 2002 to July 2003

Lab of Dr. Gluck University of Florida College o

Investigate the interaction between GAPDH and V-ATPase by using yeast two-hybrid assay and GST precipitation assay.

1995 to 1999

RESIDENCY OF INTERNAL MEDICINE

1994 to 1999

Hainan Medical College Affiliated Hospital

1994 to 1995

CLINICAL INTERNSHIP

Fellowships and Awards

Graduate Research Assistantship--University of Florida College of Medicine

Publications

1.Rongxiu Li, Yan Zang, Changyou Li, Neil S. Patel, Jennifer R. Grandis, and Daniel E. Johnson. ABT-737 synergizes with Chemotherapy to Kill Head and Neck Squamous Cell Carcinoma Cells Via a Noxa-mediated Pathway. Molecular Pharmacology 75:1231-1239, 2009.

2.Changyou Li, Rongxiu Li, Jennifer R. Grandis, and Daniel E. Johnson. Bortezomib induces apoptosis via Bim and Bik upregulation and synergizes with cisplatin in the killing of head and neck squamous cell carcinoma cells. Mol Cancer Ther 2008 June; 7(6): 647-1655

3.Rongxiu Li, Amanda L. Boehm, Michelle B. Miranda, Jennifer R. Grandis, and Daniel E. Johnson. Targeting Bcl-2 Family Members with Cell-Permeable BH3 peptides Induces Apoptosis signaling and Death in Head and Neck Squamous Cell Carcinoma Cells. Neoplasia. 2007 Oct;9(10):801-11.

4.Rongxiu Li, Xiaoping Luo, David F. Archer and Nasser Chegini. Doxycycline alters the expression of matrix metalloproteases in the endometrial cells exposed to ovarian steroids and pro-inflammatory cytokine. J Reprod Immunol. 2007 Apr; 73(2):118-29. Epub 2006 Sep 27.

5.Rongxiu Li, Xiaoping Luo, Qun Pan, Issam Zineh, David F. Archer, R.Stan Williams and Nasser Chegini. Doxycycline alters the expression of inflammatory and immune-related cytokines and chemokines in human endometrial cells: implication in irregular uterine bleeding.

Hum Reprod. 2006 Oct;21(10):2555-63. Epub 2006 Aug 4.

6.Rongxiu Li, Sanjeev Shangary, Amanda L. Boehm, Jennifer R. Grandis, and Daniel E. Johnson. Activation of cell death and apoptosis signaling in head and neck squamous cell carcinoma cells by BH3 peptides. AACR 97th Annaul Meeting 2006 April 1-5, Washington DC, Volume 47. Abstract.

7.Rongxiu Li, Amanda L. Boehm, Jennifer R. Grandis, and Daniel E. Johnson. Targeting Bcl-XL with Cell-Permeable BH3 peptides Promotes Apoptosis in Head and Neck Squamous Cell Carcinoma Cells. ( Abstract, SPORE meeting).

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