LC-MS specialist

Jun Feng Xiao

Green card holder

Email: *************@*****.***

Phone: 520-***-****

Summary of Qualifications

• A strong knowledge in small molecules and proteins based biomarker discovery, ID verification and method validation.

• Extensive training in analytical techniques with specialty in utilizing LC/MS/MS (HPLC and UPLC) for both quantitative and qualitative analyses in biological matrices.

• Independent problem solving skills, responsible and good team player.

Education

Research associate Sep.2011-present

Lombard Cancer Center, Department of Oncology

Postdoc research fellow Jul.2010 - Sep.2011

Lombard Cancer Center, Department of Oncology

Postdoc research fellow Aug.2009 - Jul.2010

University of Pennsylvania, Department of Pharmacology

Ph.D in Analytical Chemistry (GPA: 3.7/4.0) Aug.2005- Aug.2009

Rensselaer Polytechnic Institute, Troy, NY

M.S. in Analytical Chemistry Aug.2001- May 2004

Dalian Institute of Chemical Physics, Chinese Academy of Science, China

BE in Chemical Engineering & BA in English Aug.1996 - Aug.2001

Dalian University of Technology, China

Technical Skills

• Performing heat map, PCA, and OPLS-DA analysis of LC-MS data for metabolic biomarker discovery using Random Forests (RF) and SIMCA-P .

• Developing LC/ /MS/MS method for metabolic profiling, identification and quantitation in urinary, sera, tissue and cellular matrices.

• Developing label-free assay for affinity binding study using Biacore and macromolecule structure characterization by Circular Dichroism (CD).

• Biological sample preparation techniques: 96-well plates based sample preparation; liquid-liquid extraction (LLE); protein precipitation; protein enzymatic digestion; solid phase extraction (SPE) and solid phase microextraction (SPME).

• Instrumental operation and maintenance of mass spectrometers, HPLC and optical spectrometers: Waters G2 QTOF, Waters QTOF-MS Premier, Waters XEVO TQ MS; AB Sciex Qtrap 4000, AB Sciex QSTAR Elite and AB Sciex API 4000 Triple Quad; Bruker TOF/TOF-MS; Thermo LTQ-XL and TSQ Quantum AM; Waters nano-Acquity UPLC and Acquity UPLC; Waters Alliance 2690; Agilent 1100 series; Eksigent microflow HPLC; Thermo Finnigan; GE Health Biacore 3000, Bruker FTIR, PerkinElmer UV-Vis, Shimadzu CD.

Research Experiences

Lombardi Cancer Center Jul. 2010-present

• Untargeted metabolomics: metabolic profiling, feature detection, database search based identification, structural elucidation and ID verification in sera, tissue and cellular matrices using UPLC-MS.

• Targeted metabolomics: absolute quantitation of metabolites in sera, tissue and cellular matrices using isotope dilution based selected reaction monitoring (SRM) detection.

University of Pennsylvania Aug.2009-Jul.2010

• Quantification of DNA adducts in human urine using LC-SRM-MS after liquid-liquid extraction, SPE clean up and reagent derivatization.

• Purification of synthesized DNA adducts product using LC-UV.

Rensselaer Polytechnic Institute Aug.2005-Aug.2009

• Identification of DNA binding sites in proteins: micro LC/MS/MS analysis of enzymatic digested peptides and Mascot based database search for protein ID.

• Characterization of peptide sequence using LC/MS/MS and MALDI-TOF/TOF-MS/MS.

• Development of label-free assays for real-time monitoring DNA-protein binding interaction using Surface Plasmon Resonance (SPR).

Dalian Institute of Chemical Physics, CAS Aug.2001-May 2004

• Detection of chlorinated pesticides in seawater by SPME-GC-ECD and GC-MS.

• Quantification of medicinal component-melatonin from Chinese herbs by LC-Fluorescence Detection after SPE pretreatment.

Awards

• Student Fellowship, Rensselaer Polytechnic Institute, Aug.2007- May 2009

• ASMS Student Travel Award, May 2009

• ASMS Student Travel Award, May 2008

• First Prize in English Competition, Dalian Institute of Chemical Physics, Nov.2003

• Excellent Student Scholarship, Dalian University of Technology, May 1998

Membership and Activity

2012-present Member of Staff/APP Advisory Council, GUMC

2011-present Member of American Association for Cancer Research (AACR)

2010-present Consulting Member for Gerson Lehrman Group, Inc

2010-present Journal reviewer: Trends in Analytical Chemistry; Analytical Methods; Analytical and Bioanalytical Chemistry; Analytical Biochemistry.

2007-present Member of The American Society for Mass Spectrometry (ASMS)

2009-2010 Member of CSSAP (Chinese Student and Scholar Association at Penn)

2005-2009 Member of CSSA (Chinese Student and Scholar Association at RPI)

Journal Publications

• Bin Zhou, Jun Feng Xiao, Habtom Ressom. A Computational Pipeline for LC-MS/MS

Based Metabolite Identification. Proteomics, 2nd submission.

• Jun Feng Xiao, Rency S. Varghese, Bin Zhou, Mohammad R. Nezami Ranjbar, Yi Zhao, Tsung-Heng Tsai, Cristina Di Poto, Jinlian Wang, David Goerlitz, Yue Luo, Amrita Cheema, Mahlet G. Tadesse, Naglaa Sarhan, Hanah Soliman, Dina Hazem Ziada, Habtom W. Ressom. LC-MS Based Serum Metabolomic Study for Hepatocellular Carcinoma Biomarker Discovery in Egyptian Patient Cohort. Journal of Proteomics Research, accepted.

• Habtom W. Ressom, Jun Feng Xiao, Leepika Tuli, Rency S. Varghese, Bin Zhou, Tsung-Heng Tsai, Mohammad R. Nezami Ranjbar, Jinlian Wang, Cristina Di Poto, Amrita K. Cheema, Mahlet G. Tadesse, Radoslav Goldman, Kirti Shetty. Utilization of Metabolomics to Identify Serum Biomarkers for Hepatocellular Carcinoma in Patients with Liver Cirrhosis. Analytica Chimica Acta, in press.

• Leepika Tuli, Tsung-Heng Tsai, Rency S. Varghese, Jun Feng Xiao, Amrita Cheema, Habtom W. Ressom. Using a Spike-In Experiment to Evaluate Analysis of LC-MS Data. Proteome Science, 2012, 10:13.

• Jun Feng Xiao, Bin Zhou, Habtom Ressom. Metabolite Identification and Quantitation in LC-MS/MS Based Metabolomics. Trend in Analytical Chemistry, 2012, 32, 1-14.

• Bin Zhou, Jun Feng Xiao, Leepika Tuli, Habtom Ressom. LC-MS Based Metabolomics. Molecular BioSystems (shared first authorship), 2012, 8, 470-481.

• Bin Zhou, Jun Feng Xiao, Habtom Ressom. A Computational Pipeline for LC-MS/MS Based Metabolite Identification. IEEE BIBM 2011, accepted as short conference paper.

• Linda B.McGown, Jun Feng Xiao. DNA-modified ITO surfaces for affinity MALDI-MS, Analytical Methods, 2010, 2, 1160-1165.

• Jun Feng Xiao, Linda B.McGown. Mass Spectrometric Determination of ILPR G-Quadruplex Binding Sites in Insulin and IGF-2. Journal of the American Society for Mass Spectrometry, 2009, 20, 1974-82.

• Jun Feng Xiao, Jennifer A. Carter, Kimberley A. Frederick and Linda B. McGown. A Genome-Inspired DNA Ligand for Affinity Capture of Insulin and Insulin-like Growth Factor-2. Journal of Separation Science, 2009, 32, 1654-1664.

• Jun Feng Xiao, Rongbo Guo, Jiping Chen, Xinmiao Liang. Rapid Determination of Organic Chlorinated Pesticides in Seawater by SPME-GC– ECD. Journal of Instrumental Analysis (chinese edition), 2005, 21, 86-93.

Conference Presentations

• Jun Feng Xiao, Lihua Zhang, Yue Luo, Amrita Cheema, Habtom W. Ressom. A Targeted Serum Lipidomics Study by Ultra Performance Liquid Chromatography Coupled with Quadruple Linear Ion Trap Mass Spectrometer. 8th Annual International Meeting of the Metabolomics Society, Washington DC, 2012.

• Jun Feng Xiao, Lihua Zhang, Yue Luo, Amrita Cheema, Habtom W. Ressom. Target

Lipidomics in Serum Study by Ultra Performance Liquid Chromatography Coupled with

Tandem Mass Spectrometer. 60th ASMS Conference on Mass Spectrometry, Vancouver,

2012.

• Jun Feng Xiao, Lihua Zhang, Yue Luo, Amrita Cheema, Habtom W. Ressom. Target

Lipidomics in Serum Study by Ultra Performance Liquid Chromatography Coupled with tandem Mass Spectrometer. LIPID MAPS Annual Meeting 2012.

• H. W. Ressom, J. F. Xiao, L. Tuli, R. Varghese, B. Zhou, T.-H. Tsai, M. R. Nezami Ranjbar, Y. Zhao, J. L. Wang, C.DiPoto, A. K. Cheema, M.G. Tadesse, R. Goldman, K. Shetty. Metabolic Biomarkers of Hepatocellular Carcinoma. AACR Annual Meeting, Chicago 2012.

• Jun Feng Xiao and Habtom W. Ressom. A fast screening method for identification of multiple classes of metabolites in serum using UPLC-ESI-MS/ MS. International Symposium on the Separation of Proteins, Peptides & Polynucleotides (ISPPP), Alexandra, VA, 2011.

• Jun Feng Xiao and Habtom W. Ressom. Simultaneous identification and quantification of multiple classes of metabolites in serum by LC-ESI-MS/MS. 27th Asilomar Conference on Mass Spectrometry -METABOLOMICS, Pacific Grove, CA, 2011.

• Habtom W. Ressom, Jun Feng Xiao, Leepika Tuli, Rency Varghese, Bin Zhou, Tsung-Heng Tsai, Mohammad R. Nezami Ranjbar, Amrita Cheema, Mahlet G. Tadesse, Radoslav Goldman, Kirti Shetty. Metabolic Biomarkers of Hepatocellular Carcinoma. 7th Early Detection Research Network (EDRN), Bethesda, MD, 2011.

• Habtom W. Ressom, Jun Feng Xiao, Leepika Tuli, Rency Varghese, Bin Zhou, Tsung-Heng Tsai, Nezami Ranjbar, Amrita Cheema, Radoslav Goldman, Kirti Shetty. Utilization of Metabolomics to Identify Serum Biomarkers for Hepatocellular Carcinoma (HCC) in Patients with Liver Cirrhosis (LC). American Association for the Study of Liver Diseases (AASLD) Meeting, San Francisco, 2011.

• Bin Zhou; Jun Feng Xiao ; Habtom Ressom. A Computational Pipeline for LC-MS/MS Based Metabolite Identification. IEEE International Conference on Bioinformatics and Biomedicine (BIBM), Atlanta, 2011.

• Bin Zhou; Jun Feng Xiao ; Habtom Ressom. A Workflow for Metabolite Identification from LC-MS/MS data. 59th ASMS Conference on Mass Spectrometry, Denver, 2011.

• Jun Feng Xiao, Linda B.McGown. Characterization of binding sites of insulin and IGF-2 for a genome-inspired DNA binding ligand by MALDI-TOF-Mass Spectrometry. 57th ASMS Conference on Mass Spectrometry, Philadelphia, 2009.

• Jun Feng Xiao, Linda B.McGown. Affinity Binding of Insulin and IGF-2 with Genomic G-quadruplex Forming Oligonucleotides. 2nd International Meeting on Quadruplex DNA, Louisville, KY, 2009.

• Jun Feng Xiao, Linda B.McGown. Binding Studies of Genome-inspired DNA Binding Ligands for Insulin and IGF-2 by MALDI-TOF-MS. PITTCON, Chicago, 2009.

• Jun Feng Xiao, Linda B.McGown. Genome-inspired DNA binding ligands for Affinity MALDI-TOF-MS. 56th ASMS Conference on Mass Spectrometry, Denver, Colorado, 2008.

• Jun Feng Xiao, Jennifer Carter, Linda B.McGown. Genome-inspired DNA Binding Ligands for Insulin and IGF Proteins. PITTCON, New Orleans, Louisiana, 2008.

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