Cover letter

DIPANWITA DUTTA

908-***-**** *** Easton Avenue, Apt 15N, Somerset, NJ 08873 *********.**@*****.***

Results-focused professional with 6 years of industrial research experience in nutraceutical products, cosmetics, and drug discovery. Research experience includes genotoxicity assays, cancer signaling pathways, cardiovascular health, and immunomodulation. Demonstrated ability to manage major international projects, utilizing expertise in R&D methodology to ensure high product quality and timely delivery. Fully authorized to work in the United States.

EDUCATION

NATIONAL ENVIRONMENTAL ENGINEERING RESEARCH INSTITUTE, India 2006

PhD in Genetic Toxicology

Thesis: “Study on the ameliorative effect of natural products against genotoxicity causing environmental and industrial chemicals and wastes.”

Established toxic levels of environmental genotoxic chemicals to human life.

Researched the ameliorating effect of natural product against genotoxic compounds.

Identified the molecular mechanisms involved in ameliorating effect.

Elucidated the molecular mechanism involved in anticarcinogenic potential of natural products targeting leukemia cell.

JIWAJI UNIVERSITY, Gwalior, India 2001

Master of Science in Environmental Science

PROFESSIONAL EXPERIENCE

SUAN FARMA INC., Hackensack, NJ 07601 2011 – Present

QA-QC Technical Director, Product Development and Regulatory Affairs

Develop technical tools for product validation, qualification, and regulatory affairs prior to market release.

Coordinated activities to obtain company-wide cGMP certification. (Waiting to obtain cGMP).

Prepared SOPs for cGMP certification.

AVESTHAGEN LTD., Bangalore, India 2007 – 2011

Functional Head, Cell Based Assay Group

Led a 3-member team to identify the drug effects on hepatocytes for Strand life sciences.

Prepared roadmap and coordinated 5-member project team: nutritional bio-actives for cardiovascular disease for DANONE France.

Developed, optimized, and validated cell-based genotoxicity assay for nutritional bio-actives.

Shortlisted by Department of Bio-technology, Govt. of India as the principal investigator for a research proposal on “A cohesive model to screen for female reproductive failure caused by commonly encountered chemicals” (May 2011).

Profiled cytokine and chemokine assays): Inflammatory markers, including PGE2, TXB2, LTB4, iNOS, IL-1ß, and histamine release.

Performed assay standardization and validation for nutritional bio-actives, including cardiovascular health markers (Triglyderide, Cholesterol, HDL, ApoA1, ApoB100, TNF, IL-6, ENOS, CRP, ACE); immunomodulatory markers (Lymphocyte Proliferation, Cytokines Profiling); and mRNA expression studies for ApoA1 and ApoB100.

Isolated nutritional bio-actives for hepatoprotection, including rat primary hepatocytes with 70-80% viable cells and microsomal and cytosolic fraction for CYP450 and Aldehyde Oxidase activity.

Oversaw assay standardization for hepatoprotective activity.

Standardized and validated phototoxic and photo protective assays for cosmetics bio-actives.

Isolated mechanistic assays for collagen and elastin release.

DIPANWITA DUTTA PAGE TWO

PROFESSIONAL EXPERIENCE (continued)

NATURAL REMEDIES PVT. LTD., Bangalore, India 2006 – 2007

Senior Research Officer

Performed in vitro pre-clinical safety evaluation of phyto extracts, including chromosomal aberration and micronucleus assay.

Researched phyto extracts for cosmetic usage: collagen and elastin release assays and lipid peroxidation assays.

NATIONAL ENVIRONMENTAL ENG. RESEARCH INSTITUTE (NEERI), India 2001 – 2006

CSIR Senior Research Fellow (2004-2006)

Research Fellow (2001-2003)

TECHNICAL SKILLS

Cell culture: Primary culture, adherent / suspension culture cell-lines.

Molecular Biological Techniques: ELISA, DNA isolation, RNA isolation, m-RNA expression, c-DNA preparation, PCR and RT-PCR, transfection, western blotting.

Signaling pathways for cancer: Apoptotic and necrotic assays, cytotoxicity assays, Phosphatidylserine externalization, caspase dependent assays, down regulation of Bcl-2 expression, activation of caspase 9 and 3.

In-vitro genotoxicity assay: Ames test, Comet assay, chromosomal aberration and micronucleus study in mammalian cells (human whole blood culture and Chinese hamster cell line).

Cell based biochemical assays: Lipid per-oxidation in human serum, HepG2 cells and human dermal fibroblast, DPPH activity, nitric oxide activity antioxidant assay, collagenase and elastase activity, COX and LOX inhibition assay, ROS activity, Mitochondrial membrane potential activity, SOD, GPx, GST activity.

Instrumentation: Fluorescence spectrophotometer, Supercritical-cal fluid extractor (SFE), Gas chromatography Mass spectroscopy (GC-MS), High performance liquid chromatogram (HPLC), Flow Cytometry, Magnetic cell-sorter (MCS), Thermal Cycler (PCR).

JOURNAL PUBLICATIONS

Dipanwita Dutta, et al. “Effect of thymol on peripheral blood mononuclear cell PBMC and acute promyelotic cancer cell line HL-60.” Chemico-Biological Interactions, vol. 193, pp 97-106, 2011. DOI- 10.1016/j.cbi.2011.05.009.

Dipanwita Dutta, et al. “Role of Carum copticum seeds in modulating chromium-induced toxicity on human bronchial epithelial cells and human peripheral blood lymphocytes.” Experimental and Toxicologic Pathology. Accepted 23rd March. DOI:10.1016/j.etp.2011.03.012.

Dipanwita Dutta, et al, “In vitro safety evaluation of BacoMindTM on human lymphocytes.” Biomedical and Environmental Sciences, vol. 21(1), pp 7-23, 2008.

DIPANWITA DUTTA PAGE THREE

JOURNAL PUBLICATIONS (continued)

K. Krishnamurthi, Dipanwita Dutta, et al. “Genotoxicity of sludges, wastewater and effluents from three different industries.” Archives of Toxicology, vol. 82(12), pp 965-971, 2008.

Dipanwita Dutta, et al. “Anticlastogenic effect of redistilled cows urine distillate in human peripheral lymphocytes challenged with manganese dioxide and hexavalent chromium.” Biomedical and Environmental Sciences, vol. 19, 2006.

K. Kumar, S. Saravana Devi, K. Krishnamurti, Dipanwita Dutta, T. Chakraborti. “Decolorisation and detoxi-fication of Direct Blue-15 by a bacterial consortium.” Bioresource Technology, vol. 98, pp. 3168-3171, 2006.

Dipanwita Dutta, et al. “Modulatory effect of distillate of Ocimum sanctum leaf extract (Tulsi) on human lymphocytes against genotoxicants.” Biomedical and Environmental Sciences, vol. 20, pp 226-234, 2006.

Dipanwita Dutta, et al. “Protective effect of distillate and redis-tillate of cow urine in human polymorphonuclear leukocytes challenged with established genotoxic chemicals.” Biomedical and Environmental Sciences, vol. 17, pp 86-93, 2004.

Dipanwita Dutta, et al. “Antigenotoxic/Ameliorative effect of Kamdhenu Ark and Redistilled Kamdhenu Ark in human polymorphonuclear leukocytes against established geno-toxic chemicals.” Journal of Ecophysiology and Occupational Health, vol. 4, pp 27-36, 2004.

PATENTS

International patent granted by US/PCT countries: “A composition (RCUD) for protecting and/or repairing DNA from oxidative damages and a method thereof.”

International patent filed in PCT countries: “An extract of the punica species and a process for obtaining an extract thereof.”

AWARDS / ACHIEVEMENTS

Ranked First in the University in the Master of Environmental Science Programme.

CSIR Senior Research Fellow (CSIR-SRF Scheme), 2004

Gold Medal, Poster presentation at Society of Toxicology, India: “Genotoxicity of Manganese and protection of the damage using Garlic extract.”

Gold Medal, Poster presentation at The Academy of Environmental Biology: “Genotoxicity Assessment Of Direct Dye Stimulated Waste Water Extracts And Treated Water Extracts In Human Leukocyte Cells.”

Best Poster Prize at Biotalk, a symposium on CANCER: “Anticlastrogenic Apoptotis inducing property of Kamdhenu Ark against cancer cell line.”

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