Engineering Design

VIDYA SESHADRI

***, **** ** ****** ****,

#***, *********,

CA-94087.

*******@*****.*** Phone No: 408-***-****

OBJECTIVE

Seeking full time job opportunity as a Research Associate.

ACADEMIC BACKGROUND

Master of Science, Bio Engineering, Clemson University, Clemson, SC, May 2011, GPA: 3.88/4.0

Bachelor of Technology, Bio Technology, S.R.M University, Tamilnadu, India, May 2008, GPA:9.10/10.0

TECHNICAL SKILLS

Laboratory Skills

Aseptic Cell Culture Techniques, DNA/ RNA Extraction, primer designing, RT-PCR, Immunohistochemistry, ELISA, gel electrophoresis, Western Blotting, Cell based assays, Protein based Assays, Histology techniques (Paraffin embedding and sectioning, Cryosectioning and Staining), Transfection, UV/Vis spectrophotometry, Gel Permeation Chromatography, , Standard and Fluorescence Microscopy

Software skills

SolidWorks, Photoshop, AutoCAD

PROJECTS

• Master’s Thesis: Exploring the potential of inkjet printer for the fabrication of tissue test systems. Clemson University, SC, August 2009- May 2011

Standardized the cell output from bioprinter as a function of time and cell concentration using mammalian cells and analyzed cartridge performance

Fabricated bio-polymeric scaffolds and performed biocompatibility analysis by proliferation and metabolic activity studies on mesenchymal cells

Studied the effect of spatial co-location of 2 cell types on adipogenesis

lipid expression using triglyceride assay and Oil-Red-O staining

gene expression using polymerase chain reaction

• Development of a chitosan based bio ink. Clemson University, SC, May 2010-August 2010

Modified chitosan using ultrasound, making it suitable for inkjet printing

Fabricated chitosan based scaffold and chitosan-gelatin scaffold using bioprinting

Performed biocompatibility analysis using proliferation and metabolic assays with mesenchymal cells

• A study on the rheological properties of bio inks, Clemson University, SC, September 2008 – July 2009

Trained in mammalian cell culture, molecular biology techniques and bioprinting

Measurement of viscosity and surface tension of commercial inks

Studied the effect of fibroblast cell concentration on the viscosity and surface tension of bio-inks

Analyzed the effect of different viscosity modifiers on the bio-inks.

PRESENTATIONS AND TERM PAPERS

• Paper submitted to 33rd Annual International IEEE EMBS Conference entitled “ Cell settling effects on a thermal inkjet bioprinter”, April 2011

• Poster presentation at the Society For Biomaterials entitled “Bio-fabrication and cell- by-cell deposition”, April 2010

• Involved in the conception and design of a telescopic stent to eliminate the need for multiple stents in case of long lesions. Proposed design alternatives and thorough assessment of design objectives and criteria and presented sketches made with SolidWorks and AutoCAD, Spring 2009.

RELEVANT COURSES

Biomaterials Polymeric Biomaterials

Compatibility of Biomaterials Vascular Engineering and Pathology

Cell and Molecular Tissue Engineering Biomechanics

Cell Biology Tissue Engineering

PROFESSIONAL ORGANIZATIONS

• Society For Biomaterials

• Clemson Bioengineering Society ,Clemson

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