Biomedical Scientist
Resume:
ANA CRISTINA CAMARA SANTIAGO LEANDRO
San Antonio, TX 78251
e-mail:*************@*****.***
SUMMARY:
*- *********** ********* ******* ** managing a work scientific laboratory environment;
2- Nine years experience with biology laboratory operations;
3- Effective problem-solving, interpretive, multitasking, and organizational skills;
4- Excellent academic and professional background in biotechnology;
5- Hands-on experience and extensive knowledge in various techniques of Molecular Biology, Microbiology and Immunology;
6- Strong leadership qualities and interpersonal skills.
7- Personal dedication to excellence.
8- A versatile, innovative, creative, hard working, productive/goal-oriented professional with research experience in immunology and genetics in host- parasite/pathogen interactions.
EDUCATION
Doctor of Philosophy - Clinical Epidemiology of Infectious Diseases – Major: Immunogenetics - Infectious diseases, March, 2009
Oswaldo Cruz Foundation, Rio de Janeiro – Brazil – (FCSA certified)
Master of Science - Parasitology / Biology- Major: Immunology – Infectious disease- December, 2002
Oswaldo Cruz Foundation, Rio de Janeiro, Brazil - (FCSA certified)
Bachelor of Science - Biomedical Science - Infectious disease- Immunology, December, 1999
Rio de Janeiro State Federal University, Rio de Janeiro, Brazil - (FCSA certified)
LANGUAGES
Fluent in English.
Fluent in Portuguese
Good spoken and writing skills in English and Portuguese.
TEACHING EXPERIENCE
Assistant Professor – Undergraduate Biology Course, Bioethics and Biosafety.
Technical Educational Souza Marques Foundation
June 2004 – December 2005 (3 semesters)
Professor - Post Graduate Infectious Disease Course - Immunology course for Master Degree Students
Oswaldo Cruz Foundation
March 2004-December 2005
RESEARCH EXPERIENCE
Laboratory Assistant / Laboratory manager
Texas Biomedical Research Institute – Tx Biomed, San Antonio, TX
February 2006- 2009
Project title: “TB vaccine development in non-human primate model”
Summary of duties and responsibilities: Plan, assign, direct and execute work in the laboratory; Oversee operational and safety aspects of the laboratory; Manage all safety data; Conduct periodic inspection of the laboratory; Manage chemical and biological waste disposal; Coordinate and assign test requests for laboratory personnel; Review laboratory quality, productivity, cost, and reporting time lines data; Oversee assignment of inventory control of products and supplies; Coordinate all maintenance and repair of laboratory instrumentation, equipment and facility; Maintain and coordinate the quality management system documents, including standard operating procedures and test methods protocols; Revise department orientation goals as needed and ensures employee completion of department orientation; Demonstrate ability to develop and train personnel in new laboratory methods; Ability to understand, operate and troubleshoot laboratory instrumentation; Ability to effectively communicate with laboratory leadership, laboratory technicians and others as needed; Sufficient math skills to perform calculations associated with lab testing; Supervise subordinates employees.
Post Doctorate Scientist- PhD
Texas Biomedical Research Institute – Tx Biomed, San Antonio, TX
February 2009- present
Project title: “TB vaccine development in non-human primate model”
Summary of duties and responsibilities: Plan, assign, and execute work in the laboratory; Oversee operational and safety aspects of the laboratory; Manage all safety data; Coordinate and assign test requests for laboratory personnel; Maintain and coordinate quality management system documents; Review laboratory quality, productivity, cost, and reporting time lines data; Oversee assignment of inventory control of products and supplies; Revise department orientation goals as needed and ensures employee completion of department orientation; Ability to develop and train personnel in new laboratory methods; Analysis of all project results; Writing reports and publications; Supervise subordinates employees; Flow Cytometry, ELISA and ELISPOT expert; Outstanding Immunological and Mycobacteriological knowledge and skills.
Co- Investigator
Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
May 2005- Present
Project title: “Immunogenicity of new vaccines against tuberculosis in NHP: pre-clinical analysis of hsp65 DNA vaccine”
Summary of duties and responsibilities: Oversee operational and safety aspects of the project; Coordinate and execute the necessary tests for the project outcome; Ability to train new students in new laboratory methods; Ability to understand, operate and troubleshoot laboratory instrumentation; Academic laboratory experience; Proven ability to manage multiple tasks simultaneously with good analytical and decision making skills; Optical microscopy, bacterial staining, enumeration and identification of bacteria, counting techniques, sterile techniques, media and buffer preparation; Preparation of reagents for Research work with GLP guidelines, Quality control check, Labeling and Packing; maintenance of laboratory record book; Manage all safety data.
Principal Investigator
Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
June 2003- January 2006
Project title: “Identificação de polimorfismos em genes associados à imunopatologia da infecção pelo Mycobacterium tuberculosis” – Polymorphisms Identification on genes associated with the immunopathology of Mycobacterium tuberculosis”
Summary of duties and responsibilities: Responsible for the development, standardization and implementation of protocols to meet the project hypothesis; Partnered with biology colleagues in obtaining and presenting appropriate data package to gain governance committee approval to bring new targets into the project; Preparation of technical reports and recommendations based upon research project outcomes; Proven ability to manage multiple tasks simultaneously with good analytical and decision making skills; Optical microscopy, bacterial staining, enumeration and identification of bacteria, counting techniques, sterile techniques, media and buffer preparation; Preparation of reagents for research work with GLP guidelines, Quality control check, Labeling and Packing; Maintenance of laboratory record book; Managed all safety data; Performed all necessary data analysis as well as presentations.
Principal Investigator
Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
March 2001- December 2002
Project title: “Correlação entre a colonização pelos isoladoas clínicos de Mycobacterium avium e a imunidade celular específica a antígenos micobacterianos em pacientes HIV-1 positivos” – “Correlation between the colonization of mycobacterium avium clinical isolates and the cellular immune response specific to mycobacterium antigens in HIV-1 positive patients.”
Summary of duties and responsibilities: Worked on the co-infection disease immunology (TB / HIV-project for research; Optical microscopy, bacterial staining, enumeration and identification of bacteria, counting techniques, sterile technique, media and buffer preparation; Preparation of reagents for Research work; maintenance of laboratory record book; Performed all necessary data analysis as well as presentations.
SUPERVISORY EXPERIENCE
Supervisor/Mentor – Post graduation in Microbiology – Miriam Beatriz Goulard
Project Title: Identification of virulence factors in clinical Mycobacterium avium clinical isolates. 2003
Supervisor/Mentor – Post graduation in Microbiology – Marcia Andrade da Rocha
Project Title: Identification of DPB gene SNP in tuberculosis patients at Rio de janeiro state. 2005
Co- supervisor/Mentor – M.S. – Marcia Andrade da Rocha
Project Title: Identification of DPB and vitamin D gene SNP in tuberculosis patients. 2006-2007
Co-supervisor/Mentor – undergraduate student – Milena Rosalba
Project Title: Identification of DPB gene SNP in tuberculosis patients at Rio de Janeiro state. 2007
Co- supervisor/Mentor – P.h.D. – Marcia Andrade da Rocha
Project Title: Screening host and M. tuberculosis genes expression on dendritic cells upon vitamin D regulation.
2008-2012
Laboratory Supervisor – TxBiomed - Assistant Researcher – Magdalena Cepeda de la Garza -2008-2010.
Laboratory Supervisor – TxBiomed - Assistant Researcher – Kruti Murthy -2006-2008.
Laboratory Supervisor – TxBiomed - Assistant Researcher – Monique Molina -2006-2006.
PRESENTATIONS AND POSTERS
SANTIAGO-LEANDRO ACC, Rocha MA, Jomori K, VandeBerg JL, Rolla VC, Bonecini-Almeida. Lack of association in vitamin D receptor polymorphisms in tuberculosis patients. In: XXXIV Brazilian Immunology Society Meeting and X Allergy and Clinical Immunology International Symposium - Salvador, Bahia; 2009.
Lima, MR; Costa, LC; SANTIAGO-LEANDRO, ACC; Barradas, MM; Souza, AL; Bruck, MA; Bonecini-Almeida MG. Evaluation of the phenotype and cellular activation status basis in captive non-human primates (Macaca fascicularis and Macaca Mulatta) for preclinical vaccine studies. In: XXXIV Brazilian Immunology Society Meeting and X Allergy and Clinical Immunology International Symposium - Salvador, Bahia; 2009.
SANTIAGO-LEANDRO ACC, Murthy K, Dick E, Hubard G, Hodara VL, Giavedoni L, Brasky KM, De la Garza M, Silva CL, Bonecini-Almeida MG, VandeBerg JL. A single dose of prime-booster DNA-hsp65 and DNA-encoding antigen TDM-loaded microspheres vaccine elicited both CD4 and CD8 activated T cells in non-human primate model. In: The third International Conference on TB vaccines for the world, 2008, Atlanta, GE. The third International Conference on TB vaccines for the world/CDC, 2008.
SANTIAGO-LEANDRO ACC, Murthy K, Dick E, Hubard G, Hodara VL, Giavedoni L, Brasky KM, De la Garza M, Silva CL, Bonecini-Almeida MG, VandeBerg JL. Natural history of virulent Mycobacterium tuberculosis Erdman strain infection in rhesus monkeys.. In: The third International Conference on TB vaccines for the world, 2008, Atlanta, GE. The third International Conference on TB vaccines for the world/CDC, 2008
SANTIAGO-LEANDRO ACC. Correlation between Mycobacterium avium complex colonization and the specific cellular immune response to mycobacterium antigens in HIV-1 patients. In: XXVII Brazilian Immunology Society Meeting, 2002, Salvador. XXVII Brazilian Immunology Society Meeting.
SANTIAGO-LEANDRO ACC, Bonecini-Almeida MG. Control of Mycobacterium bovis BCG Growth in HIV-1 monocytic cell line. In: XX Brazilian Microbiology Meeting, 1999, Salvador. Annals of XX Brazilian Microbiology Meeting, 1999. p. 425.
SANTIAGO-LEANDRO ACC, Bonecini-Almeida MG. Control of Mycobacterium bovis BCG Growth in HIV-1 monocytic cell line. In: II Brazilian HIV/Aids Basic Research Symposium, 1999, Ilha de Comandatuba. Revista do Instituto de Medicina Tropical de São Paulo, 1999. v. 41. p. p.S1-S72.
PUBLICATIONS
Martineau AR*, SANTIAGO-LEANDRO ACC*, Anderson ST, Newton SM, Wilkinson KA, Mark P, Path N, Pienaar SM, Skolimowska KH, Rocha MA, Rolla VC, Levin M, Davidson RN, Bremner SA, Griffiths CJ, Eley BS, Bonecini-Almeida MG, Wilkinson RJ. Association between Gc genotype and susceptibility to TB is dependent on vitamin D stat. The European Respiratory Journal, v. 10, p. 10, 2010. * equally contributed to the work.
SANTIAGO-LEANDRO ACC, Rocha MA, Bonecini-Almeida MG. Genetic polymorphisms in vitamin D receptor, vitamin D-binding protein, Toll-like receptor 2, nitric oxide synthase 2, and interferon-gamma genes and its association with susceptibility to tuberculosis. Brazilian Journal of Medical and Biological Research, v. 42, p. 312-322, 2009
Albuquerque MC, Aleixo AL ; Benchimol EI, SANTIAGO-LEANDRO ACC, Neves LB, Vicente RT, Bonecini-Almeida MG, Amendoeira MRR. The IFN-g +874T/A gene polymorphism is associated with retinochoroiditis toxoplasmosis susceptibility. Memórias do Instituto Oswaldo Cruz, v. 104, p. 451-455, 2009.
COURSES
1) BSL-3 training – How to work in a BSL-3 environment. – 2007 and 2010.
2) Good Laboratory Practice – Texas Biomedical Research Institute – 2006, 2008 and 2010.
3) Working with the IACUC on the AALS Learning library – 2007 and 2010.
4) Laboratory Biosafety Courses
5) Flow Cytometry Course- 2002 and 2011
6) Tuberculosis Intensive – Heartland National TB Center – San Antonio – Texas - 2010
TECHNICAL EXPERIENCE:
1) Immunology Techniques (ELISA, ELISPOT, Flow Cytometry (intracellular and surface cell receptors, Proliferation assay, apoptosis, etc);
2) Molecular Biology techniques (DNA/RNA extraction, PCRs, DNA/RNA electrophoresis, SNPs, RT-PCR)
3) Cellular Biology (Cell Isolation and Culture, Fluorescence and optical microscopy);
4) Microbiology Techniques (Bacterial culturing, Mycobacteria growth and isolation, CFU counting, etc);
5) Non- human primates (Rhesus monkeys – Macaca Mulatta and cynomolgus – Macaca Fascicularis) and human blood- handling and processing biological samples;
6) Work in a sterile environment;
7) Biosafety specialist.
SOFTWARE EXPERIENCE:
1) Windows XP, Vista and 7.0
2) Apple - Mac
3) Microsoft office: Word, Excel, PowerPoint, Outlook and Access
4) Statistical Programs (Epi-info, SPSS, SNPstat)
5) Image programs: COREL DRAW and PHOTOSHOP
6) Internet Explorer or Google Chrome
7) PUBMED, SciELO (scientific search sites)
WORK REFERENCES:
Dr John VandeBerg, PhD – Scientific Director of Texas Biomedical Research Institute/ SNPRC director
Phone: 210-***-****
***@****************.***
Edward J. Dick, Jr., D.V.M., diplomate ACVP
Phone: 210-***-****
*****@********.***
Melissa de la Garza, Assistant Veterinarian / ABSL-3 Facility Scientific Manager
Phone: 21-258-9574
********@********.***
Magdalena Cepeda de la Garza
Virology Research Assistant - Texas Biomedical Research Institute
Phone: 210-***-****
*******@********.***
If you need recommendation letters fell free to contact me at anytime or contact directly the phone numbers or e-mails above.
Ana Cristina Leandro
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