MICROBIOLOGIST

Reena Kuruvilla

*** **** ***** ***** *****, Seffner, FL- 33584. Home Ph: 813-***-****. Cell Ph: 813-***-****. E-mail: ************@*****.***

OBJECTIVE

To obtain a position as a Microbiologist, that will enable me to utilize my

scientific background and training to perform research into biological

systems and contribute to research and development.

EXPERIENCE

Medical Technologist 2009 to present

Brandon Regional Hospital, Brandon, FL

1. Collect and prepare specimens and perform laboratory procedures used in the diagnosis, treatment and prevention of disease and report lab results on all performed tests

2. Accurate, reliable, diligent and focused on the timely, quality completion of all lab procedures.

Microbiology Technician 2006 to 2009

Bausch & Lomb Pharmaceutical, Inc.

1. Practical experience in isolation and detection of metal particles from

pharmaceutical products by membrane filtration method and using accuizer.

2. Preparation of different types of media for the isolation of

Microorganism.

Microbiology Instructor 2007 to 2008

Keiser University, Lakeland, FL

1. Provide individualized instruction to students in microbiology course &

Lab in nursing program.

Microbiology Analyst 2005 to 2006

EMSL Analytical Lab, Orlando, FL

1. Experience on identification of fungal spores (fungal analysis) by

direct microscopy and handling various mycological and microbiological

techniques in aseptic conditions in lab environment.

2. Practical experience for isolation of microbial species by culturing

techniques

3. DNA extraction by PCR technology

Lab Assistant 2004 to 2006

Hillsborough Community College, Tampa, FL

1. Responsible for making common Medias, solutions, buffer, reagents, and

plate stock for the entire lab.

2. Responsible for performing HPLC, Gas chromatography, L.C, titration and

variety of wet chemistry techniques used to analyze different product.

3. Responsible for handling instruments, such as Calorimeter,

Spectrophotometer meter, Gel electrophoresis, Ultra centrifuge, etc.

4. Assist graduate students in lab work and prepare cultures for

microbiology lab classes.

Biology Instructor 2004 to 2005

Sanford Brown Institute, Tampa, FL

1. Provide individualized instruction to students in introductory biology

course in Cardio vascular technology program.

2. Gained experience in teaching topics such as Biodiversity, Heredity,

Genetics, Evolution, and Basic biological principles of living things.

Lecturer 2001 to 2004

Kesari Arts and Science College, Kerala, India

Worked as a lecturer in the Department of microbiology at Kesari Art and

Science College. Kesari Art and Science College is a branch of Mahatma

Gandhi University and specializes in teaching subjects such as Molecular

Biology, General microbiology, Environmental microbiology, Food and

Industrial Microbiology, Immunology, Medical Microbiology, Recombinant DNA

Technology, Biotechnology, Clinical Microbiology, Physiology, and

Parasitology.

1. Conducted research in particular field of knowledge and publishes

findings in professional journals.

2. Served on the faculty committee, providing professional consulting

services to government and industry.

3. Directed, research of other teachers and graduate students working for

advanced academic degrees.

Senior Microbiologist 2001 to 2004

Endocrinology and Immunology Lab. Kerala, India

Gained practical experience in Bacteriology which includes culturing of

bacteria, Biochemical testing, Antibiotic sensitivity test,

Immuno-diagnostic test, Bacterial examination of water by multiple tube

fermentation test, Staining techniques, Handling and use of different

microscopes, Observing bacterial motility, Direct agglutination test RBC,

Direct bacterial agglutination test, Widal test, Weil Felix test, Brucella

agglutination test, Cold agglutination test, VDRL, Isolation of

immunoglobulin, serum electrophoresis, Immunoelectrophoresis, Counter

immunoelectrophoresis, Bacteriophage amplification test, Polymerase chain

reaction for the detection of HIV, HCV, TB, CMV, Dengue viruses, and ELISA.

1. Cut images of chromosomes from photograph and identified and arranged

them in numbered pairs on karyotype chart, using standard practices.

2. Calibrated and maintained equipment used in quantitative and

qualitative analysis, such as spectrophotometers, calorimeters, flame

photometers, and computer-controlled analyzers.

3. Conducted chemical analysis of body fluids, including blood, urine, and

spinal fluid, to determine presence of normal and abnormal components.

4. Selected and prepared specimen and media for cell culture, using

aseptic technique and knowledge of medium components and cell requirements.

Microbiologist 2001 to 2002

Pushpagari Hospital Kerala, India

1. Gained practical experience in preparing culture media, blood grouping,

and Rh typing, Bacterial count of samples containing bacteria, Culturing

techniques, Biochemical tests, and Serological tests.

2. Cut, stains, and mounted biological material on slides for microscopic

study and diagnosis, following standard laboratory procedures.

3. Analyzed, samples of biological material for chemical content and

reaction.

4. Harvested cell culture at optimum time sequence based on knowledge of

cell cycle differences and culture conditions.

Project Assistant 2001

A project was completed in Gram-negative bacteria associated with White

Spot Syndrome in Penaeus indicus. White Spot Syndrome is one of the most

dreaded diseases in Shrimp Aquaculture. The organism responsible for this

disease has caused mass mortalities of shrimp in India, Thailand, China,

and Japan. This virus (WSSV) is a rod shaped enveloped, nonocculated

virion. Due to the infection of this virus there is a rapid reduction in

food consumption, lethargy, a loose cuticle and a pink to reddish brown

discoloration of the hepatopancrease. The course of infection by WSSV leads

to a profound impact of opportunistic pathogens, particularly an invasion

by Vibrio, Alteromonas, Aeromonas, Photo bacterium, Enterobacterialceae,

and Pseudomonas. This indicates that viral infection weakens host defense

mechanism considerably enabling the entry of secondary pathogens.

Food Microbiologist: 2002 to 2002

Naveen Spices Ltd. Kottayam, Kerala, India

1. Specialized, in microbiological quality assurance for food industry.

2. Gained experience in food analysis using chemical and immunological

techniques.

Practical Experience:

Soil & Food Microbiology: Gained valuable experience in Enumeration of

Microbial population of soil, Isolation of Azatobacter, Azospirillium

species from soil, Isolation of Rhizobium species from root nodules of

legumes, cultivation of Rhizobium species, Nodulation of legume

roots-Leonard Jar Experiment, Isolation of Cyano bacteria, Phosphobacteria,

Actinomycetes, and ammonification and Nitrification of soil. Gained

valuable experience in the Bacteriological examination of water

(qualitative test), Standard plate count (Quantitative test), Membrane

filter technique, Detection of Coliform bacteria in water, Membrane filter

technique for Coliform group, Bacterial counts of food samples, Isolation

of Lipolytic organism from butter, Reductase test, Microbiological

examination of soft drinks, and Analysis of Bacterial food borne diseases.

Medical Microbiology and Immunology; Gained valuable experience in

Ouchlerlony's Double Immunodiffusion, Slide and tube agglutination test,

Blood grouping and Rh typing, White blood cells study: Differential blood

count, Isolation and identification of Gram negative and Gram positive

bacteria, Isolation and identification of urinary tract pathogens, Primary

stool analysis, Biochemical test, and Quantitative determination of anti

microbial sensitivity.

Microbial Genetics: Gained valuable experience in the Isolation of

chromosomal DNA from Escherichia coli, Isolation of plasma DNA, Bacterial

conjugation, Ampicillin treatment for isolation of Auxotrophs, Bacterial

mutagenicity and carcinogenesis, Ames test, Polymerase Chain Reaction for

the detection of HIV, HCV, and CMV, Bacteriophage amplification test for

the detection of Mycobacterium tuberculosis.

EDUCATION

M.S., Microbiology 2001

University of Bharathiar/Sri Ramakrishna College of Arts and Science

Coimbatore, India

B.S., Microbiology 1999

University of Madras Madras, India

PROFESSIONAL LICENSES

Certified Clinical Laboratory Technologist by the American Medical Technologists(AMT) Lic.#230025

State of Florida Certified Clinical Laboratory Technologist(Lic.# TN42430)

ACADEMIC ACHIEVEMENTS

Extracurricular Achievements - Received Rajapuraskar Award of Guides and

also received several awards for sports activities and computer knowledge.’

REFERENCE

Upon request

Contact this candidate