Polymerize chain reaction, elisa, care stream imaging, gels...

Sridevi Vempati

**** * **** ******, *** ***, Norfolk, VA 23508

ntld0y@r.postjobfree.com / ntld0y@r.postjobfree.com

Phone number # 270-***-****

Summary:

. Willing to relocate to Virginia and nearby states, since husband

recently moved to Virginia.

. Over 4 years of experience as a laboratory technician in

Molecular biology/ Microbiology labs.

. Experience of working in BSLII and BSL III level labs.

. Expert in PCR based detection of DNA/ RNA of the bacteria or

viruses causing diseases.

. Good working knowledge of isolation and identification of

bacteria, viruses and fungi using laboratory methods.

. Completed advanced education in Molecular Biology, Medical

Microbiology, Immunology, Biochemistry and Biotechnology.

. Willing to learn new methods and techniques in the field of

Molecular Biology.

. Active member of the audit team and quality & safety committee

member.

. Data entry of results using specific software programs.

. Team player, excellent communication and administrative skills,

self-motivated to work hard.

Education:

. Medical microbiology course in summer semester 2008 at

Kentucky Community And Technical College System,

Hopkinsville, KY

. Eastern Kentucky University, Richmond, KY, USA, Master of

Public health, July 2006

. Kakatiya University, Warangal, India. M.Sc (Biotechnology),

April 2002

Relevant courses: Advanced Molecular Biology, Biotechnology,

Genetics, Immunology,

Biochemistry,

. Kakatiya University, India. B.Sc. (Industrial Microbiology),

April 2000.

Relevant courses: Industrial Microbiology, Biology, Botany,

Chemistry, Zoology,

Genetics.

Experience:

A. 02/2010 - present Molecular Diagnostics Laboratory Technician

II, at Breathitt veterinary center (BVC), Murray

state university, Hopkinsville, KY.

The BVC is fully accredited for all animal species by the

American association of veterinary laboratory diagnosticians

(AAVLD). The scientific disciplines of Pathology, Microbiology,

Molecular Diagnostics, Toxicology, Serology, and Virology are

provided in the facility to provide rapid, accurate diagnostic

data.

Responsibilities:

. Different extraction methods include spin column method by using

Qiagen extraction, Tetra core, Rneasy mini kits, Heat block and

Magnetic bead methods (Ambion).

. Running PCR by using different types of Thermo cyclers

(Conventional & Real time & High Through put), by using

different types of PCR's include single, multiplex, nested, qRT

pcr's,, gel electrophoresis,

. Running PCR's for detection of E.coli toxins and adhesion

factors, Clostridium toxin profiling Salmonella, abortion cases

for Listeria, Leptospira, Neospora, and Toxoplasma, Trichmonas,

chronic wasting Disease (MAP), Johne's testing, Step. equi,

equine herpes virus 1& 4 PCR's, bovine viral diarrheal virus PCR,

west nile virus, bulk tank milk testing for Mycoplasma and

Mycoplasma panels for bovine, avian, and pigs.

. Running high thorough put 7500 fast Applied Bio systems RT

Thermocyler for avian influenza virus surveillance, classical

swine fever surveillance, foot and mouth disease surveillance,

avian paramyxo and New castle disease surveillance PCR'S

. Detection of conventional Thermo cycler products by using gel

electrophoresis and visualizing and analyzing by Carestream

molecular Imaging software bye 212PRO gel logic imager.

. Aseptic techniques and accurate detailed data entering results

into specific software.

. In addition to the above would able to handle Western blot,

Elisa, Agarose gel Immuno diffusion(AGID), Immuno Fluorescent

Antibody testing(IFA), Agglutination and Micro agglutination,

Spectrophotometer, Biologs, Sensititer, compound, electron and

Dark field fluorescent microscopes.

. Perform mastitis cultures, bulk tank milk testing, and a variety

of special cultures including Johne's disease

. Bacterial and fungal Isolation and Identification by using

biochemical test, Id panels, Bio logs.

B. 08/2006-03/2007: Regional Epidemiologist, Purchase district

health department, Mayfield, KY.

C. 1/2000 - 8/2003: Research assistant at Discovery Biotechnology

lab, Indian Institute of Chemical Technology, (IICT), Hyderabad,

India

Responsibilities:

. Bacterial and fungal Isolation and Identification by using

biochemical test, Detection of bacterial, viral and fungal

diseases using PCR, and other techniques.

. Assist project manager to set up isolate, identify, read and

run sample of Aspergillus Terrus cultures to estimate the

lovastatin production from the specific fungal cultures, by

using Shake Flask Fermenter, UV Spectrophotometer to read

results.

. To handle day-to-day cell culturing protocols and maintenance of

cells.

. Use of immunoblotting, immunofluorescence, and tissue culture

cloning for various projects.

Awards:

. Kakatiya University Gold medalist, M.Sc. (Biotechnology) -

March/April, 2002.

. Best Outgoing Student - S.R. & B.G.N.R Government Degree College

- April 2000.

. Gopal Reddy Gold Medal (for standing first in Sciences) - S.R. &

B.G.N.R. degree College - April 2000.

References:

Dr. Shri Singh, DVM,

Head of the department for microbiology and molecular Diagnostics,

Murray state university, Breathitt veterinary center,

715 north drive,

Hopkinsville, KY 42240

Tel: 270-***-****

Email: ntld0y@r.postjobfree.com.

Dr. Louis Pittman, DVM

Head of pathology department,

Murray state university, Breathitt veterinary center,

715 north drive

Hopkinsville, KY 42240

Tel: 270-***-****

Email: ntld0y@r.postjobfree.com

Dr. Ramesh Gupta, DVM

Head of the department Toxicology,

Murray State University, Breathitt Veterinary Center,

715 North Drive

Hopkinsville, KY 42240

Tel: 270-***-****

Email: ntld0y@r.postjobfree.com

Dr. Cheryl Guyer, DVM

Pathologist,

Murray State University, Breathitt Veterinary Center,

715 North Drive

Hopkinsville, KY 42240

Tel: 270-***-****

Email: ntld0y@r.postjobfree.com

Contact this candidate